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The map label for this gene is nplT [H]

Identifier: 145300569

GI number: 145300569

Start: 3989656

End: 3991272

Strand: Direct

Name: nplT [H]

Synonym: ASA_3701

Alternate gene names: 145300569

Gene position: 3989656-3991272 (Clockwise)

Preceding gene: 145300568

Following gene: 145300572

Centisome position: 84.84

GC content: 63.2

Gene sequence:

>1617_bases
ATGAAACAAGGACGTTTGCGCTATGGCGGCCTGCTCGGGGCCGCCTTGTTTCTGACGGCGTGCGGCAGCGGGGGCGGTGG
CTCCAGCAGCGAGGCTCAGCCACCCGTGGTCAATCCACCGGTGACCTCTGCCAGCCGCGCCTGTTATGGCAATGACCAGG
CCGCCTGCAACCTGAGAACCTATCAGGTGATGGTGGAGTCTTTCGTCGATGGCGACAGCGCCGCCAACTACGGCGTCGGT
TATGGCCCCTCCGGGCACAACGGCGATCTGCAAGGGATCATCGACAGCCTGGATCACATCAAGAGTCTCAACGTCAACGC
CATCTGGCTCACCCCTGTATTTGACTCCTGCGCGGGTCAGGGGGGGGATGACAAGCTGGATGCTACCGGCTACTTCGCCT
GTGATTTTTTCACCGTGGATCCCAACTTTGGCAGCAATGCCCAGCTCAAACAGCTGGTGGATGCGGCGCACCAGCGCGGC
CTTTACGTCTTCCTCGATGGCGTGTTCGGTCACGTCAACAAGGTGGGAGTGAGCAAGCCGTCGCCAGAGGGGCGCCAGCC
TGCGCTCAAGAGCGGGGGGGCCGGTTATCCCGGTCAGCTGGTGGATTACAGCCAACCCGAGAGCCTCGCCTACTTCAAGG
AGGTGGCGCGTTACTGGGTCGAACAGTACGGCATCGACGGCTGGCGACTGGATCAGGCCTATCAGCTGGGGCTGGACGAA
TGGCGTGCCATTCGCGGCGAAGTCGAACAGGCCAGTGCGGCACGCAAGGCGGCGGGCCAACAGTGGGGCACCCTGGGCTA
CATGGTGGGTGAGGTATGGAAGAGTGCTGACGAGATCCGGGCCCAGGCCTATGGCCCGAGCGACAAGCCGGCGCTCTCCT
CGGCATTCGACTTCCCGCTGCGCTACGGCATAGTACAGGCGCTGGCGGTGGAGGAGTCCGGCAAGGGCGGGCAGGGGGCC
AGCGTGCTGGATGCCAGCTGGAACAAGGTGGAGAGCTATCCGGACCACGCCATGCCCAATCTGATGCTGGGCAACCACGA
TTTGGTACGCTTCGGTGACCTGCTGGAGCGGGGAAATTTCAATCCGGCTGACTACTGGCAGCGCCACAAGGCGGCCTTCA
GCTTCCTCGCGGCTCGCTCAGGCCCCATCACCTTCTATTACGGCGAGGAGTTTGGCGACGAGGTGCCTGGCTTTGCCAAG
CAGGTGAGTGGCGACTGTGTGGCGCAGGGACTCTGTGACGATCACGTGGCCCGCAGCGATGGCAAGGTGCCCGGTGTCAC
CGGTTTCGTGCCGAGCAGCGATCAGGCCGAGCTCAAGCAGTGGCTCAGCCAGTTGCTGGCGATGCGCGCCACGCATCCCG
CCCTCTATCAGGGGGAGCGAGTCAAGCTGGTGGCCGATGGCAGCGTTTATGGCGACATCAAACAGACGGCGGCAGAGCAG
ATTGTCTACCTGCTCAACGTCTCCACCACGCCTCAAAGCTATGCCCTGCCGGTCGGCAAACTGCGCAGTGGCAGTGCCCT
GGTGGATCTGCAAAGCGGCGAGAGTCTGGCTCTGGGTGGCAGCAGCGTGACGGTGGACTTGCCACCGCTGAGCGGTCGCT
TCCTGCTGTTGCAATAA

Upstream 100 bases:

>100_bases
GGGTAACGTTTTCAGTCACAGACCCGCAGTGGTGAGCTGGGTATCATGGCTTCAAGGGTGGCGCGCTCTGCGCCAGCACC
ACCACAGCGGGAAGATAACA

Downstream 100 bases:

>100_bases
GGCTGGCGAGTAACAACAAGGGCTCCAGTTTGGAGCCCTTGTTCATTGTGGTGGGTTACTTCTCGATACCGCTGCCAAGC
TCGTCCTGATGGGTATTGGC

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MKQGRLRYGGLLGAALFLTACGSGGGGSSSEAQPPVVNPPVTSASRACYGNDQAACNLRTYQVMVESFVDGDSAANYGVG
YGPSGHNGDLQGIIDSLDHIKSLNVNAIWLTPVFDSCAGQGGDDKLDATGYFACDFFTVDPNFGSNAQLKQLVDAAHQRG
LYVFLDGVFGHVNKVGVSKPSPEGRQPALKSGGAGYPGQLVDYSQPESLAYFKEVARYWVEQYGIDGWRLDQAYQLGLDE
WRAIRGEVEQASAARKAAGQQWGTLGYMVGEVWKSADEIRAQAYGPSDKPALSSAFDFPLRYGIVQALAVEESGKGGQGA
SVLDASWNKVESYPDHAMPNLMLGNHDLVRFGDLLERGNFNPADYWQRHKAAFSFLAARSGPITFYYGEEFGDEVPGFAK
QVSGDCVAQGLCDDHVARSDGKVPGVTGFVPSSDQAELKQWLSQLLAMRATHPALYQGERVKLVADGSVYGDIKQTAAEQ
IVYLLNVSTTPQSYALPVGKLRSGSALVDLQSGESLALGGSSVTVDLPPLSGRFLLLQ

Sequences:

>Translated_538_residues
MKQGRLRYGGLLGAALFLTACGSGGGGSSSEAQPPVVNPPVTSASRACYGNDQAACNLRTYQVMVESFVDGDSAANYGVG
YGPSGHNGDLQGIIDSLDHIKSLNVNAIWLTPVFDSCAGQGGDDKLDATGYFACDFFTVDPNFGSNAQLKQLVDAAHQRG
LYVFLDGVFGHVNKVGVSKPSPEGRQPALKSGGAGYPGQLVDYSQPESLAYFKEVARYWVEQYGIDGWRLDQAYQLGLDE
WRAIRGEVEQASAARKAAGQQWGTLGYMVGEVWKSADEIRAQAYGPSDKPALSSAFDFPLRYGIVQALAVEESGKGGQGA
SVLDASWNKVESYPDHAMPNLMLGNHDLVRFGDLLERGNFNPADYWQRHKAAFSFLAARSGPITFYYGEEFGDEVPGFAK
QVSGDCVAQGLCDDHVARSDGKVPGVTGFVPSSDQAELKQWLSQLLAMRATHPALYQGERVKLVADGSVYGDIKQTAAEQ
IVYLLNVSTTPQSYALPVGKLRSGSALVDLQSGESLALGGSSVTVDLPPLSGRFLLLQ
>Mature_538_residues
MKQGRLRYGGLLGAALFLTACGSGGGGSSSEAQPPVVNPPVTSASRACYGNDQAACNLRTYQVMVESFVDGDSAANYGVG
YGPSGHNGDLQGIIDSLDHIKSLNVNAIWLTPVFDSCAGQGGDDKLDATGYFACDFFTVDPNFGSNAQLKQLVDAAHQRG
LYVFLDGVFGHVNKVGVSKPSPEGRQPALKSGGAGYPGQLVDYSQPESLAYFKEVARYWVEQYGIDGWRLDQAYQLGLDE
WRAIRGEVEQASAARKAAGQQWGTLGYMVGEVWKSADEIRAQAYGPSDKPALSSAFDFPLRYGIVQALAVEESGKGGQGA
SVLDASWNKVESYPDHAMPNLMLGNHDLVRFGDLLERGNFNPADYWQRHKAAFSFLAARSGPITFYYGEEFGDEVPGFAK
QVSGDCVAQGLCDDHVARSDGKVPGVTGFVPSSDQAELKQWLSQLLAMRATHPALYQGERVKLVADGSVYGDIKQTAAEQ
IVYLLNVSTTPQSYALPVGKLRSGSALVDLQSGESLALGGSSVTVDLPPLSGRFLLLQ

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=129, Percent_Identity=34.8837209302326, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790687, Length=207, Percent_Identity=28.0193236714976, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 57489; Mature: 57489

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQGRLRYGGLLGAALFLTACGSGGGGSSSEAQPPVVNPPVTSASRACYGNDQAACNLRT
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHH
YQVMVESFVDGDSAANYGVGYGPSGHNGDLQGIIDSLDHIKSLNVNAIWLTPVFDSCAGQ
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHCCC
GGDDKLDATGYFACDFFTVDPNFGSNAQLKQLVDAAHQRGLYVFLDGVFGHVNKVGVSKP
CCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEECHHHHHHHCCCCCC
SPEGRQPALKSGGAGYPGQLVDYSQPESLAYFKEVARYWVEQYGIDGWRLDQAYQLGLDE
CCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECHHHHHCHHH
WRAIRGEVEQASAARKAAGQQWGTLGYMVGEVWKSADEIRAQAYGPSDKPALSSAFDFPL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHCCHH
RYGIVQALAVEESGKGGQGASVLDASWNKVESYPDHAMPNLMLGNHDLVRFGDLLERGNF
HHHHHHHHHHHCCCCCCCCCEEECCCHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCC
NPADYWQRHKAAFSFLAARSGPITFYYGEEFGDEVPGFAKQVSGDCVAQGLCDDHVARSD
CHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCCHHHHHCCHHHHHCCCHHHHHCCC
GKVPGVTGFVPSSDQAELKQWLSQLLAMRATHPALYQGERVKLVADGSVYGDIKQTAAEQ
CCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCEEEEEECCCCHHHHHHHHHHH
IVYLLNVSTTPQSYALPVGKLRSGSALVDLQSGESLALGGSSVTVDLPPLSGRFLLLQ
EEEEEECCCCCCHHCCCCCCCCCCCEEEEECCCCEEEECCCEEEEECCCCCCCEEEEC
>Mature Secondary Structure
MKQGRLRYGGLLGAALFLTACGSGGGGSSSEAQPPVVNPPVTSASRACYGNDQAACNLRT
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHH
YQVMVESFVDGDSAANYGVGYGPSGHNGDLQGIIDSLDHIKSLNVNAIWLTPVFDSCAGQ
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHCCC
GGDDKLDATGYFACDFFTVDPNFGSNAQLKQLVDAAHQRGLYVFLDGVFGHVNKVGVSKP
CCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEECHHHHHHHCCCCCC
SPEGRQPALKSGGAGYPGQLVDYSQPESLAYFKEVARYWVEQYGIDGWRLDQAYQLGLDE
CCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECHHHHHCHHH
WRAIRGEVEQASAARKAAGQQWGTLGYMVGEVWKSADEIRAQAYGPSDKPALSSAFDFPL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHCCHH
RYGIVQALAVEESGKGGQGASVLDASWNKVESYPDHAMPNLMLGNHDLVRFGDLLERGNF
HHHHHHHHHHHCCCCCCCCCEEECCCHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCC
NPADYWQRHKAAFSFLAARSGPITFYYGEEFGDEVPGFAKQVSGDCVAQGLCDDHVARSD
CHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCCCCHHHHHCCHHHHHCCCHHHHHCCC
GKVPGVTGFVPSSDQAELKQWLSQLLAMRATHPALYQGERVKLVADGSVYGDIKQTAAEQ
CCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCEEEEEECCCCHHHHHHHHHHH
IVYLLNVSTTPQSYALPVGKLRSGSALVDLQSGESLALGGSSVTVDLPPLSGRFLLLQ
EEEEEECCCCCCHHCCCCCCCCCCCEEEEECCCCEEEECCCEEEEECCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]