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The map label for this gene is ispB [H]

Identifier: 145300249

GI number: 145300249

Start: 3638974

End: 3639945

Strand: Direct

Name: ispB [H]

Synonym: ASA_3364

Alternate gene names: 145300249

Gene position: 3638974-3639945 (Clockwise)

Preceding gene: 145300243

Following gene: 145300259

Centisome position: 77.39

GC content: 60.08

Gene sequence:

>972_bases
ATGGACCAACAGACTATCCGTGCGCTCTGTGCTGCCGACATGACGGCGGTGAATGAACTGATCCTGGCGAGACTTCAGTC
CGATGTCAGCCTGATCAACCAGCTGGGTTTCTATATTGTCAGTGCAGGCGGGAAACGGATGCGCCCCATGCTGACAGTGA
TGGCCGCCCGCGCACTGGGATACGAAGGTCAGGATCATCTCAAACTCGCCGCCATCATCGAATTCATTCATACCTCTACC
CTGCTGCACGACGACGTGGTCGACGAATCCGATCTGCGCCGTGGCCGGGAGACCGCCAATGCGCTGTTTGGCAATGCTGC
CAGCGTGCTGGTCGGCGACTACCTCTACAGCCGCTCCTTCCAGATGATGAGCGAGCTTTCCAACCTGCGAGTGATGGAGA
TCCTGTCGGATGCCACCAATACCATCGCAGAGGGGGAAGTGTTGCAGCTGATGAACTGCAACGATCCGGACACCACAGAA
CAGAGCTACATGACGGTCATCTACTGCAAGACCGCCAAACTGTTCGAGGCCGCCACCCGCCTCGCGGCCGTGCTGACCCA
TCAGCCCGAGCCCGTTGAGCAGGCTATGACCGACTACGGCAAGTATCTGGGCACAGCCTTCCAGATCATCGATGACGTCA
TGGACTACTGCTCCCAGAGCGATGAGATGGGCAAGAACGTCGGTGACGATCTGGCCGAAGGCAAGCCGACCCTGCCGCTG
CTACGGGCCATGGAAGTGGGCTCACCCGAAGAGCGCCAGCTGGTGCGTGATGCCATCGAGCATCGCAATGGCATGGAACA
TCTCGACCAGATTCTCGGGATTCTCGACCGCACCGGTGCGCTGGAGTACTCCCGCATGCGTGCCAGGGAAGAGGCTGACA
AGGCCATTGCCGCCCTCGCCATCCTGCCGGACTCCCAGCACAAGCAGGCGCTGGAAACCCTGGCCCAGATGGCGGTACAA
CGCAGCGCCTGA

Upstream 100 bases:

>100_bases
CGCCGTGAATAAACGCTTAACTGTAACCCATTGTGAAATCGTGTAGAATCCCCGCCATTACTAAAACCGCAGTTATTACT
GCCGTCACGTTACGAGACTT

Downstream 100 bases:

>100_bases
GTCAGCGATATCTGCTGTTGCATAAAAAAAGAGGCCTCGGGGCCTCTTTTTTATTGTCTGTGCTGCAATGGAACTCAGAG
CCAGCCACATGATTCAGTGA

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MDQQTIRALCAADMTAVNELILARLQSDVSLINQLGFYIVSAGGKRMRPMLTVMAARALGYEGQDHLKLAAIIEFIHTST
LLHDDVVDESDLRRGRETANALFGNAASVLVGDYLYSRSFQMMSELSNLRVMEILSDATNTIAEGEVLQLMNCNDPDTTE
QSYMTVIYCKTAKLFEAATRLAAVLTHQPEPVEQAMTDYGKYLGTAFQIIDDVMDYCSQSDEMGKNVGDDLAEGKPTLPL
LRAMEVGSPEERQLVRDAIEHRNGMEHLDQILGILDRTGALEYSRMRAREEADKAIAALAILPDSQHKQALETLAQMAVQ
RSA

Sequences:

>Translated_323_residues
MDQQTIRALCAADMTAVNELILARLQSDVSLINQLGFYIVSAGGKRMRPMLTVMAARALGYEGQDHLKLAAIIEFIHTST
LLHDDVVDESDLRRGRETANALFGNAASVLVGDYLYSRSFQMMSELSNLRVMEILSDATNTIAEGEVLQLMNCNDPDTTE
QSYMTVIYCKTAKLFEAATRLAAVLTHQPEPVEQAMTDYGKYLGTAFQIIDDVMDYCSQSDEMGKNVGDDLAEGKPTLPL
LRAMEVGSPEERQLVRDAIEHRNGMEHLDQILGILDRTGALEYSRMRAREEADKAIAALAILPDSQHKQALETLAQMAVQ
RSA
>Mature_323_residues
MDQQTIRALCAADMTAVNELILARLQSDVSLINQLGFYIVSAGGKRMRPMLTVMAARALGYEGQDHLKLAAIIEFIHTST
LLHDDVVDESDLRRGRETANALFGNAASVLVGDYLYSRSFQMMSELSNLRVMEILSDATNTIAEGEVLQLMNCNDPDTTE
QSYMTVIYCKTAKLFEAATRLAAVLTHQPEPVEQAMTDYGKYLGTAFQIIDDVMDYCSQSDEMGKNVGDDLAEGKPTLPL
LRAMEVGSPEERQLVRDAIEHRNGMEHLDQILGILDRTGALEYSRMRAREEADKAIAALAILPDSQHKQALETLAQMAVQ
RSA

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=241, Percent_Identity=33.6099585062241, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI4758430, Length=265, Percent_Identity=26.4150943396226, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI83700220, Length=265, Percent_Identity=26.4150943396226, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=61.3707165109034, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1786623, Length=232, Percent_Identity=30.1724137931034, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=330, Percent_Identity=29.0909090909091, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319475, Length=299, Percent_Identity=31.438127090301, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325188, Length=227, Percent_Identity=27.3127753303965, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24651612, Length=298, Percent_Identity=29.5302013422819, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI281365769, Length=256, Percent_Identity=25, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24660002, Length=256, Percent_Identity=25, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35723; Mature: 35723

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQQTIRALCAADMTAVNELILARLQSDVSLINQLGFYIVSAGGKRMRPMLTVMAARALG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHC
YEGQDHLKLAAIIEFIHTSTLLHDDVVDESDLRRGRETANALFGNAASVLVGDYLYSRSF
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QMMSELSNLRVMEILSDATNTIAEGEVLQLMNCNDPDTTEQSYMTVIYCKTAKLFEAATR
HHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCHHEEEEEEECHHHHHHHHHH
LAAVLTHQPEPVEQAMTDYGKYLGTAFQIIDDVMDYCSQSDEMGKNVGDDLAEGKPTLPL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHCCCCCHHH
LRAMEVGSPEERQLVRDAIEHRNGMEHLDQILGILDRTGALEYSRMRAREEADKAIAALA
HHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ILPDSQHKQALETLAQMAVQRSA
CCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDQQTIRALCAADMTAVNELILARLQSDVSLINQLGFYIVSAGGKRMRPMLTVMAARALG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHC
YEGQDHLKLAAIIEFIHTSTLLHDDVVDESDLRRGRETANALFGNAASVLVGDYLYSRSF
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QMMSELSNLRVMEILSDATNTIAEGEVLQLMNCNDPDTTEQSYMTVIYCKTAKLFEAATR
HHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCHHEEEEEEECHHHHHHHHHH
LAAVLTHQPEPVEQAMTDYGKYLGTAFQIIDDVMDYCSQSDEMGKNVGDDLAEGKPTLPL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHCCCCCHHH
LRAMEVGSPEERQLVRDAIEHRNGMEHLDQILGILDRTGALEYSRMRAREEADKAIAALA
HHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ILPDSQHKQALETLAQMAVQRSA
CCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]