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The map label for this gene is rrmA [H]

Identifier: 145300208

GI number: 145300208

Start: 3585900

End: 3586700

Strand: Direct

Name: rrmA [H]

Synonym: ASA_3319

Alternate gene names: 145300208

Gene position: 3585900-3586700 (Clockwise)

Preceding gene: 145300207

Following gene: 145300211

Centisome position: 76.26

GC content: 63.42

Gene sequence:

>801_bases
ATGCATCTTCAATGTCCCGTTTGCCGCAGCCCCCTGCATCTTCATGAAGCATCCCGCGGGGTCTATTGCGACAACAAGCA
CCATTTCGATCCGGCCCCGGAAGGGTATCTGGATCTCATTCCCGGCAAGAAGAATCCCAAGGACAGCGACAGCCGCGCCC
TGATGCGCGCCAAGCGCCACTTCCTGGAACAGGGCCATCAGGCGCCGCTGGTAGACGCCATGGCTGCCCTGCTCGCCCCG
CTCGGCCCCCGCGAGCTGATCCACCTCGGCTGCGGCGAAGGTTACTACTGCCGTGCCCTGGCCGAGCGCCTGCCCGAATG
GCAATTTGGCGGATTCGATCTGGCCAAGAACGCCATCTTCGCCGCCAACAAGGTACAGCCTGAAGCTCAGTTCATCATCG
CCGATCCGGCTCGTGCCCCGCTGCAACCGGGCACGGCCCAGGTGCTGCTGGTCAATGATCTCAAGGTCAAGACAGAGCTG
CTGGTCAGCTGGCTGGCACCGGGTGGTTACCTGCTTTACCTGCAGCCAGGCCCGCGCCACCAGTGGCAGCTGAGAGTCAG
CCTCAACCCTGTCAGCATGGAGCACCCCCTCAGCTGGCCAGTCCTCGGCGGCCTCAACCCCGTGGGTCAGGAGCGTTGTC
AGTTCACCATGACCATGGATCAGAGCCTGCGCAGCTTCATTCTGGAAACCAGCCGACTCGGCTGGCGGGCCAGCGCGGAG
CAGCGTCACGCCTTTACCCAGCAGGGACCGGATGAGCTGGAGCAGGATCTGCTGCTGAGCCTGTGGCAAAAGCCGCTCTG
A

Upstream 100 bases:

>100_bases
CTGACGGCTCAGGATAAAAAAGGCTGCCACGGCAGCCTTTTCTGATCGTCTCCCCTATAATCGCGAACGCCCTTCCTCAG
CCTCAATCAAGCGAGTGTGT

Downstream 100 bases:

>100_bases
TTGCGCTCATTCCCATGAAAAAAGGCCCTGACGGGCCTTTTTTATTGAGCGAATACAGACGTCACAGTTTGATCCAGGCA
TCTCCCCAGGCCGTGCCCTT

Product: ribosomal RNA large subunit methyltransferase A

Products: NA

Alternate protein names: 23S rRNA m1G745 methyltransferase; rRNA (guanine-N(1)-)-methyltransferase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MHLQCPVCRSPLHLHEASRGVYCDNKHHFDPAPEGYLDLIPGKKNPKDSDSRALMRAKRHFLEQGHQAPLVDAMAALLAP
LGPRELIHLGCGEGYYCRALAERLPEWQFGGFDLAKNAIFAANKVQPEAQFIIADPARAPLQPGTAQVLLVNDLKVKTEL
LVSWLAPGGYLLYLQPGPRHQWQLRVSLNPVSMEHPLSWPVLGGLNPVGQERCQFTMTMDQSLRSFILETSRLGWRASAE
QRHAFTQQGPDELEQDLLLSLWQKPL

Sequences:

>Translated_266_residues
MHLQCPVCRSPLHLHEASRGVYCDNKHHFDPAPEGYLDLIPGKKNPKDSDSRALMRAKRHFLEQGHQAPLVDAMAALLAP
LGPRELIHLGCGEGYYCRALAERLPEWQFGGFDLAKNAIFAANKVQPEAQFIIADPARAPLQPGTAQVLLVNDLKVKTEL
LVSWLAPGGYLLYLQPGPRHQWQLRVSLNPVSMEHPLSWPVLGGLNPVGQERCQFTMTMDQSLRSFILETSRLGWRASAE
QRHAFTQQGPDELEQDLLLSLWQKPL
>Mature_266_residues
MHLQCPVCRSPLHLHEASRGVYCDNKHHFDPAPEGYLDLIPGKKNPKDSDSRALMRAKRHFLEQGHQAPLVDAMAALLAP
LGPRELIHLGCGEGYYCRALAERLPEWQFGGFDLAKNAIFAANKVQPEAQFIIADPARAPLQPGTAQVLLVNDLKVKTEL
LVSWLAPGGYLLYLQPGPRHQWQLRVSLNPVSMEHPLSWPVLGGLNPVGQERCQFTMTMDQSLRSFILETSRLGWRASAE
QRHAFTQQGPDELEQDLLLSLWQKPL

Specific function: Specifically methylates the guanosine in position 745 of 23S rRNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=270, Percent_Identity=31.8518518518519, Blast_Score=133, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.187 [H]

Molecular weight: Translated: 29843; Mature: 29843

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLQCPVCRSPLHLHEASRGVYCDNKHHFDPAPEGYLDLIPGKKNPKDSDSRALMRAKRH
CCCCCCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHH
FLEQGHQAPLVDAMAALLAPLGPRELIHLGCGEGYYCRALAERLPEWQFGGFDLAKNAIF
HHHCCCCCCHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHEE
AANKVQPEAQFIIADPARAPLQPGTAQVLLVNDLKVKTELLVSWLAPGGYLLYLQPGPRH
ECCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCC
QWQLRVSLNPVSMEHPLSWPVLGGLNPVGQERCQFTMTMDQSLRSFILETSRLGWRASAE
EEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHEEEHHHHHHHHHHHHHHHCCCCCCCHH
QRHAFTQQGPDELEQDLLLSLWQKPL
HHHHHHCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHLQCPVCRSPLHLHEASRGVYCDNKHHFDPAPEGYLDLIPGKKNPKDSDSRALMRAKRH
CCCCCCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHH
FLEQGHQAPLVDAMAALLAPLGPRELIHLGCGEGYYCRALAERLPEWQFGGFDLAKNAIF
HHHCCCCCCHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHEE
AANKVQPEAQFIIADPARAPLQPGTAQVLLVNDLKVKTELLVSWLAPGGYLLYLQPGPRH
ECCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCC
QWQLRVSLNPVSMEHPLSWPVLGGLNPVGQERCQFTMTMDQSLRSFILETSRLGWRASAE
EEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHEEEHHHHHHHHHHHHHHHCCCCCCCHH
QRHAFTQQGPDELEQDLLLSLWQKPL
HHHHHHCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984109; 9097040; 9278503; 9440525 [H]