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The map label for this gene is yegQ [H]

Identifier: 145300174

GI number: 145300174

Start: 3552233

End: 3553594

Strand: Direct

Name: yegQ [H]

Synonym: ASA_3284

Alternate gene names: 145300174

Gene position: 3552233-3553594 (Clockwise)

Preceding gene: 145300173

Following gene: 145300175

Centisome position: 75.54

GC content: 60.5

Gene sequence:

>1362_bases
ATGTTCAGACCAGAATTGCTCTCCCCCGCCGGGACCCTCAAGAATATGCGTTACGCCTATGCCTATGGCGCCGACGCCGT
CTATGCGGGTCAGCCACGCTACAGCCTGCGGGTGCGTAACAACGAATTCGATCACGAGAATCTGCAGCTCGGCATCAACG
AGGCCCACGCGTTGGGCAAGCAGTTCTATGTGGTGGCCAACATCCAGCCCCACAACTCCAAGCTCAAGACCTTTATCCGC
GACATGCGTCCCGTGGTGGAGATGAAGCCGGATGCGCTGATCATGTCAGACCCGGGCCTTATCATGATGATGCGCGAAGC
CTTCCCGGACATGCCCATTCACCTCTCGGTGCAGGCCAACGCGGTCAACTGGGCGACGGTGAAGTTTTGGCATCAGATGG
GCCTGACCCGCGCCATCCTGTCGCGCGAGCTGTCACTTGACGAGATCGAAGAGATCCGCATGCAGGTGCCGGAGATGGAG
CTGGAAGTCTTCGTCCACGGCGCCCTGTGCATGGCCTACTCGGGCCGCTGCCTGCTGTCCGGTTACATCAACAAGCGCGA
CCCCAACCAGGGTACCTGCACTAACTCCTGTCGCTGGGAGTACAAGGTGCACGAAGGCAAGGAAGATGACGTGGGCCAGA
TAGTCCATCGCCAGGAGCCCATCGCCGTGCGTCCGGACAACACTTTGGGCCTTGGCAAACCGAGCGATGCGCTGGTGCTG
CTGGAAGAGTCCAACCGTCCCGGTGAATACATGACGGCGTTCGAGGATGAGCACGGCACCTACATCATGAACTCCAAGGA
CTTGCGCGCCGTCGAGCACGTGGAGCGCCTGACCAAGATGGGGGTGCATTCGCTCAAAATTGAAGGCCGTACCAAGTCCT
TCTACTACTGCGCCCGCACCGCCCAGGTCTATCGCAAGGCCATCGACGATGCAGTCGCCGGCCGTCCGTTCGATCCGACC
CTGATGGGCACCCTCGAAAATCTGGCCCACCGTGGCTACACCGAGGGCTTCCTGCGCCGTCACAACCACAGCGAGTACCA
GAACTACGATTACGGCTACTCGGTCTCCGACACCCAGCAGTTCGTCGGCGAGATCACCGGTCGCCAAGGCGACATGGTGG
AAGTGGAAGTGAAGAACAAGTTCCTGGTGGGCAACAGCCTGGAGCTGATGACGCCGCAGGGCAACGTCAGCTTCAATCTG
GAGCAGATGCAGAACCGCAAGGGCGAGCTCATCGACACGGCGCCGGGCAACGGCCACGTTGTCTATGTGCCCGTTCCCAA
AGAGATCGACATCAACTACGGCATATTGCTGCGCAACCTCGGCGATCGCCAGGACACCCGTCAGCCGCACTCGGCGGCCT
GA

Upstream 100 bases:

>100_bases
GGTAGTTGTGACGCGAGCGCCGTCTCGCACCACGGCTCAACCAATCCGGCGCCATCAGGTGGCGTCGTCCTGATTTGCAA
TCAGATACTAAGAGTCCAAC

Downstream 100 bases:

>100_bases
TATGTCCCTGCTCATCACCGACAAGTGCATCAACTGCGACATGTGCGATCCCGAGTGTCCGAACCAGGCAATCAGCCTGG
GGGAGGAGATCTACGAGATA

Product: U32 family peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MFRPELLSPAGTLKNMRYAYAYGADAVYAGQPRYSLRVRNNEFDHENLQLGINEAHALGKQFYVVANIQPHNSKLKTFIR
DMRPVVEMKPDALIMSDPGLIMMMREAFPDMPIHLSVQANAVNWATVKFWHQMGLTRAILSRELSLDEIEEIRMQVPEME
LEVFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNSCRWEYKVHEGKEDDVGQIVHRQEPIAVRPDNTLGLGKPSDALVL
LEESNRPGEYMTAFEDEHGTYIMNSKDLRAVEHVERLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAVAGRPFDPT
LMGTLENLAHRGYTEGFLRRHNHSEYQNYDYGYSVSDTQQFVGEITGRQGDMVEVEVKNKFLVGNSLELMTPQGNVSFNL
EQMQNRKGELIDTAPGNGHVVYVPVPKEIDINYGILLRNLGDRQDTRQPHSAA

Sequences:

>Translated_453_residues
MFRPELLSPAGTLKNMRYAYAYGADAVYAGQPRYSLRVRNNEFDHENLQLGINEAHALGKQFYVVANIQPHNSKLKTFIR
DMRPVVEMKPDALIMSDPGLIMMMREAFPDMPIHLSVQANAVNWATVKFWHQMGLTRAILSRELSLDEIEEIRMQVPEME
LEVFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNSCRWEYKVHEGKEDDVGQIVHRQEPIAVRPDNTLGLGKPSDALVL
LEESNRPGEYMTAFEDEHGTYIMNSKDLRAVEHVERLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAVAGRPFDPT
LMGTLENLAHRGYTEGFLRRHNHSEYQNYDYGYSVSDTQQFVGEITGRQGDMVEVEVKNKFLVGNSLELMTPQGNVSFNL
EQMQNRKGELIDTAPGNGHVVYVPVPKEIDINYGILLRNLGDRQDTRQPHSAA
>Mature_453_residues
MFRPELLSPAGTLKNMRYAYAYGADAVYAGQPRYSLRVRNNEFDHENLQLGINEAHALGKQFYVVANIQPHNSKLKTFIR
DMRPVVEMKPDALIMSDPGLIMMMREAFPDMPIHLSVQANAVNWATVKFWHQMGLTRAILSRELSLDEIEEIRMQVPEME
LEVFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNSCRWEYKVHEGKEDDVGQIVHRQEPIAVRPDNTLGLGKPSDALVL
LEESNRPGEYMTAFEDEHGTYIMNSKDLRAVEHVERLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAVAGRPFDPT
LMGTLENLAHRGYTEGFLRRHNHSEYQNYDYGYSVSDTQQFVGEITGRQGDMVEVEVKNKFLVGNSLELMTPQGNVSFNL
EQMQNRKGELIDTAPGNGHVVYVPVPKEIDINYGILLRNLGDRQDTRQPHSAA

Specific function: Unknown

COG id: COG0826

COG function: function code O; Collagenase and related proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U32 family [H]

Homologues:

Organism=Escherichia coli, GI1788397, Length=452, Percent_Identity=78.7610619469027, Blast_Score=745, Evalue=0.0,
Organism=Escherichia coli, GI87081910, Length=410, Percent_Identity=27.5609756097561, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789548, Length=320, Percent_Identity=28.75, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001539 [H]

Pfam domain/function: PF01136 Peptidase_U32 [H]

EC number: 3.4.-.- [C]

Molecular weight: Translated: 51493; Mature: 51493

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS01276 PEPTIDASE_U32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRPELLSPAGTLKNMRYAYAYGADAVYAGQPRYSLRVRNNEFDHENLQLGINEAHALGK
CCCCCCCCCCHHHHCCEEEEEECCCEEEECCCCEEEEECCCCCCCCCEEECCHHHHHCCC
QFYVVANIQPHNSKLKTFIRDMRPVVEMKPDALIMSDPGLIMMMREAFPDMPIHLSVQAN
EEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEHHHCCCCCEEEEEEEC
AVNWATVKFWHQMGLTRAILSRELSLDEIEEIRMQVPEMELEVFVHGALCMAYSGRCLLS
CEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEEHEEEEHCCCCEEEE
GYINKRDPNQGTCTNSCRWEYKVHEGKEDDVGQIVHRQEPIAVRPDNTLGLGKPSDALVL
ECCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHCCCCEEECCCCCCCCCCCCCEEEE
LEESNRPGEYMTAFEDEHGTYIMNSKDLRAVEHVERLTKMGVHSLKIEGRTKSFYYCART
EECCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHHH
AQVYRKAIDDAVAGRPFDPTLMGTLENLAHRGYTEGFLRRHNHSEYQNYDYGYSVSDTQQ
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHH
FVGEITGRQGDMVEVEVKNKFLVGNSLELMTPQGNVSFNLEQMQNRKGELIDTAPGNGHV
HHHHHCCCCCCEEEEEECCEEEECCCEEEECCCCCEEEEHHHHHCCCCCEEECCCCCCEE
VYVPVPKEIDINYGILLRNLGDRQDTRQPHSAA
EEEECCCEECCCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MFRPELLSPAGTLKNMRYAYAYGADAVYAGQPRYSLRVRNNEFDHENLQLGINEAHALGK
CCCCCCCCCCHHHHCCEEEEEECCCEEEECCCCEEEEECCCCCCCCCEEECCHHHHHCCC
QFYVVANIQPHNSKLKTFIRDMRPVVEMKPDALIMSDPGLIMMMREAFPDMPIHLSVQAN
EEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEHHHCCCCCEEEEEEEC
AVNWATVKFWHQMGLTRAILSRELSLDEIEEIRMQVPEMELEVFVHGALCMAYSGRCLLS
CEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEEHEEEEHCCCCEEEE
GYINKRDPNQGTCTNSCRWEYKVHEGKEDDVGQIVHRQEPIAVRPDNTLGLGKPSDALVL
ECCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHCCCCEEECCCCCCCCCCCCCEEEE
LEESNRPGEYMTAFEDEHGTYIMNSKDLRAVEHVERLTKMGVHSLKIEGRTKSFYYCART
EECCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHHH
AQVYRKAIDDAVAGRPFDPTLMGTLENLAHRGYTEGFLRRHNHSEYQNYDYGYSVSDTQQ
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHH
FVGEITGRQGDMVEVEVKNKFLVGNSLELMTPQGNVSFNLEQMQNRKGELIDTAPGNGHV
HHHHHCCCCCCEEEEEECCEEEECCCEEEECCCCCEEEEHHHHHCCCCCEEECCCCCCEE
VYVPVPKEIDINYGILLRNLGDRQDTRQPHSAA
EEEECCCEECCCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]