Click here to switch to the map view.

The map label for this gene is budR [H]

Identifier: 145300111

GI number: 145300111

Start: 3473887

End: 3474798

Strand: Direct

Name: budR [H]

Synonym: ASA_3212

Alternate gene names: 145300111

Gene position: 3473887-3474798 (Clockwise)

Preceding gene: 145300109

Following gene: 145300112

Centisome position: 73.87

GC content: 65.13

Gene sequence:

>912_bases
ATGGAATTTCGTTACCTGCGCTACCTGGTAGCCGTGGCAGAGAAGCAGAGCTTTACCAAGGCGGCCGAGACGCTGGGCAT
GGCCCAGCCGCCCCTCAGCCAGCAGATCCGCAAATTTGAACAGGAGGTGGGCACGCCACTGTTTCACCGGCTGACCCGCA
GTGTGGAGCTGACCGAGGCGGGGCTGAGCCTCTATCAGGATGCCAAAGGCATACTGTCGCAAACCGAGCAGGCCATGCAG
AAGGCCCAGCGCATCGCCCGCGGTCAGCTTGGCTCCCTGCGCCTGGGCTTTGCCGGCTCCACTGTGCTGCACCCGAGCGT
GCTGCGGCTCATCCACGACTTTCGCCAGGCCTGGCCAGAGGTGAGCCTGCATCCCATCGAGAACAGCATGCCCAATCTGG
TGCGCGACCTGATGAGCGAGGAGCTGGATGCCGCCATACTGCGCCTGCCGTGCAGCAGCAGCTCGGCGCTGACTCACCAT
CTCATCGATGAGGAGGAGATGCTGATCGCCCTCCCCCCGCACCACCCGCTGGCAAGCACGGGCGCCATCGCCATCGAGGC
CCTGGCGCAGGATCCCTTCATCCTGTTCCCGCGCGAACTGGGGCCGGGCCTGCACGATGCCATCACCCAACGCTGCCAGC
GGGCCGGTTTCAGTCCCCGTCTCGGCAGCCAGAGCCCGCAGGTGATCTCCACCGTCGGCATGGTGCAGGCCGGTTTCGGG
GTTGCCATCATCCCGCGATCTGTGAGCGAGCTGCATCCCCACGGCGTCAGCTATCACCCCATCGCCGATGAACCGCTCAC
CACCCGCATCAGCCTCGCCACCCGCCGCCATCCCCGTCAAAAGAGCCTGCAGCACCTGATAGGGCTGATCCCGAGCCGGC
ATGATAGACACACCGACATCGCATCGGCATAG

Upstream 100 bases:

>100_bases
ACCTCCAATCCTGTTAGGTTGGCAAATCAGGATTCAATAGTGACCAAGATCACACTTTGATACCAATATCCAAACCCATC
AGCTTCGAGACGTACCAGAT

Downstream 100 bases:

>100_bases
CGCCAGCCCCCGCACACCTTCATCTGCGTTCCACCAATAGACCACCACGCTGTCAGTGACAGCCTGGTGGCGCCTTGCAA
TTGAACCCAAGCGAATCGCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Bud operon transcriptional regulator [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MEFRYLRYLVAVAEKQSFTKAAETLGMAQPPLSQQIRKFEQEVGTPLFHRLTRSVELTEAGLSLYQDAKGILSQTEQAMQ
KAQRIARGQLGSLRLGFAGSTVLHPSVLRLIHDFRQAWPEVSLHPIENSMPNLVRDLMSEELDAAILRLPCSSSSALTHH
LIDEEEMLIALPPHHPLASTGAIAIEALAQDPFILFPRELGPGLHDAITQRCQRAGFSPRLGSQSPQVISTVGMVQAGFG
VAIIPRSVSELHPHGVSYHPIADEPLTTRISLATRRHPRQKSLQHLIGLIPSRHDRHTDIASA

Sequences:

>Translated_303_residues
MEFRYLRYLVAVAEKQSFTKAAETLGMAQPPLSQQIRKFEQEVGTPLFHRLTRSVELTEAGLSLYQDAKGILSQTEQAMQ
KAQRIARGQLGSLRLGFAGSTVLHPSVLRLIHDFRQAWPEVSLHPIENSMPNLVRDLMSEELDAAILRLPCSSSSALTHH
LIDEEEMLIALPPHHPLASTGAIAIEALAQDPFILFPRELGPGLHDAITQRCQRAGFSPRLGSQSPQVISTVGMVQAGFG
VAIIPRSVSELHPHGVSYHPIADEPLTTRISLATRRHPRQKSLQHLIGLIPSRHDRHTDIASA
>Mature_303_residues
MEFRYLRYLVAVAEKQSFTKAAETLGMAQPPLSQQIRKFEQEVGTPLFHRLTRSVELTEAGLSLYQDAKGILSQTEQAMQ
KAQRIARGQLGSLRLGFAGSTVLHPSVLRLIHDFRQAWPEVSLHPIENSMPNLVRDLMSEELDAAILRLPCSSSSALTHH
LIDEEEMLIALPPHHPLASTGAIAIEALAQDPFILFPRELGPGLHDAITQRCQRAGFSPRLGSQSPQVISTVGMVQAGFG
VAIIPRSVSELHPHGVSYHPIADEPLTTRISLATRRHPRQKSLQHLIGLIPSRHDRHTDIASA

Specific function: Regulator of the budABC operon for 2,3-butanediol synthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=280, Percent_Identity=34.6428571428571, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI2367136, Length=270, Percent_Identity=30, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788887, Length=301, Percent_Identity=30.5647840531561, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790399, Length=305, Percent_Identity=31.1475409836066, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI157672245, Length=281, Percent_Identity=29.1814946619217, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788297, Length=253, Percent_Identity=30.0395256916996, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787530, Length=257, Percent_Identity=29.9610894941634, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693105, Length=244, Percent_Identity=24.5901639344262, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786401, Length=169, Percent_Identity=30.7692307692308, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787601, Length=290, Percent_Identity=26.8965517241379, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790262, Length=278, Percent_Identity=28.0575539568345, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789173, Length=144, Percent_Identity=36.1111111111111, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788481, Length=292, Percent_Identity=26.027397260274, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787589, Length=184, Percent_Identity=31.5217391304348, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081904, Length=214, Percent_Identity=28.0373831775701, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786713, Length=307, Percent_Identity=24.1042345276873, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787128, Length=143, Percent_Identity=27.972027972028, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790208, Length=284, Percent_Identity=25.7042253521127, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789204, Length=245, Percent_Identity=23.265306122449, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33448; Mature: 33448

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS50931 HTH_LYSR ; PS00539 PYROKININ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFRYLRYLVAVAEKQSFTKAAETLGMAQPPLSQQIRKFEQEVGTPLFHRLTRSVELTEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GLSLYQDAKGILSQTEQAMQKAQRIARGQLGSLRLGFAGSTVLHPSVLRLIHDFRQAWPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCC
VSLHPIENSMPNLVRDLMSEELDAAILRLPCSSSSALTHHLIDEEEMLIALPPHHPLAST
CEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCC
GAIAIEALAQDPFILFPRELGPGLHDAITQRCQRAGFSPRLGSQSPQVISTVGMVQAGFG
HHHHHHHHHCCCEEEECHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
VAIIPRSVSELHPHGVSYHPIADEPLTTRISLATRRHPRQKSLQHLIGLIPSRHDRHTDI
EEECCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC
ASA
CCC
>Mature Secondary Structure
MEFRYLRYLVAVAEKQSFTKAAETLGMAQPPLSQQIRKFEQEVGTPLFHRLTRSVELTEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GLSLYQDAKGILSQTEQAMQKAQRIARGQLGSLRLGFAGSTVLHPSVLRLIHDFRQAWPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCC
VSLHPIENSMPNLVRDLMSEELDAAILRLPCSSSSALTHHLIDEEEMLIALPPHHPLAST
CEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCC
GAIAIEALAQDPFILFPRELGPGLHDAITQRCQRAGFSPRLGSQSPQVISTVGMVQAGFG
HHHHHHHHHCCCEEEECHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
VAIIPRSVSELHPHGVSYHPIADEPLTTRISLATRRHPRQKSLQHLIGLIPSRHDRHTDI
EEECCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCC
ASA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA