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The map label for this gene is mltB [H]

Identifier: 145299737

GI number: 145299737

Start: 3016737

End: 3017825

Strand: Direct

Name: mltB [H]

Synonym: ASA_2814

Alternate gene names: 145299737

Gene position: 3016737-3017825 (Clockwise)

Preceding gene: 145299736

Following gene: 145299739

Centisome position: 64.15

GC content: 60.88

Gene sequence:

>1089_bases
ATGCGTCGCCTTGTCTCCGTGCTCACTCTTTCCCTGTTTCCCCTGCTCTACGGTTGCAGCAGCACGCCTGCCCCCAAACC
CGTGGCCAAGCCGTTCAAAAGTGCCTTCATCAATCAGACGCCACAAATAAAACCTGCTTACAGCCAAGGGGTTAGCAAGG
GAGACTTCGCCAATCGCAACAACGTGGACAGCTTCGTCCAACGCATGGTCGAGAAGCACGGTTTTGACAGCGTCGAGCTG
CATCGACTGCTGGCACAGGCGGATCGCTATGACTGGATAATACGGCTGATGGATGCCCAGGCCCCCACCACCGCCAAGCC
AATCGGACCGACCGGCGCCTGGAATCGCTACCGCGCCAAGTTCATCACGCCGGACAACGTGCAAAATGGCGTCAACTTCT
GGCGTGAGTACGAGAGCGAGCTCAACCGTGCCGAGCAGGAGTATGGGGTGCCGCCCGAAATCATCGTCGGCATCATAGGC
GTGGAGACCCGCTGGGGTCGGGTAATGGGCAAGACCCGCATTCTGGATGCGCTTGCGACCCTGGCCTTCGACTATCCGCG
CCGCGCCGAATTCTTTACCGGCGAACTGGAGGCCTTCCTGGTGATGACCCGGGATGAGGGGATGGACCCCACCGAACCCA
AGGGCTCCTACGCCGGCGCCATGGGGTATGGCCAGTTCATGCCATCGAGCTTCCAGCGCTACGCGGTGGACTTTGACGGC
AATGGCCACACCAACTTGTGGGATCCGGTGGATGCCATCGGCAGCGTGGCCAACTACTTCAAGGCGCATGGCTGGGAGCA
GGGTCAACCGGTAGCGGTGCGTGGTCAGGGCCAGCTGCCCGGCTATGAGCATGGATTCCAGACCCGTTACCCGATCACCA
CCTTGCACCGCGCCGGTCTGAATCCCATCAGCAACCTTGGCAACCACAGCGAAGCAAGCCTGCTGCGCCTGGATGTCGGC
ACCGGCTATCAATACTGGTACGGCCTGCCCAACTTCTACACCATTACCCGCTACAACCACAGCACCCACTACGCCATGGC
GGTGTGGCAACTGGGGCTGGCGGTCAAGGCTGCCAGAGGCGGTTACTGA

Upstream 100 bases:

>100_bases
CAGGCCACGAGTTTGAGCCCTCATCCATGCACTGAGCACTTGCGCATGGATGACAATGTTTTACCATGACGACACTCTTT
TTCAGGCTCATGAGAACCTT

Downstream 100 bases:

>100_bases
GTCAGGCTGCTTGCCAATAAAAACGGGCCCCGCGGGCCCGTTTTTATTACTCGTTTTCCAGCAACCAGCGTTTGAGCAGG
GCAGCCAACTGAGCCTGTTC

Product: murein hydrolase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MRRLVSVLTLSLFPLLYGCSSTPAPKPVAKPFKSAFINQTPQIKPAYSQGVSKGDFANRNNVDSFVQRMVEKHGFDSVEL
HRLLAQADRYDWIIRLMDAQAPTTAKPIGPTGAWNRYRAKFITPDNVQNGVNFWREYESELNRAEQEYGVPPEIIVGIIG
VETRWGRVMGKTRILDALATLAFDYPRRAEFFTGELEAFLVMTRDEGMDPTEPKGSYAGAMGYGQFMPSSFQRYAVDFDG
NGHTNLWDPVDAIGSVANYFKAHGWEQGQPVAVRGQGQLPGYEHGFQTRYPITTLHRAGLNPISNLGNHSEASLLRLDVG
TGYQYWYGLPNFYTITRYNHSTHYAMAVWQLGLAVKAARGGY

Sequences:

>Translated_362_residues
MRRLVSVLTLSLFPLLYGCSSTPAPKPVAKPFKSAFINQTPQIKPAYSQGVSKGDFANRNNVDSFVQRMVEKHGFDSVEL
HRLLAQADRYDWIIRLMDAQAPTTAKPIGPTGAWNRYRAKFITPDNVQNGVNFWREYESELNRAEQEYGVPPEIIVGIIG
VETRWGRVMGKTRILDALATLAFDYPRRAEFFTGELEAFLVMTRDEGMDPTEPKGSYAGAMGYGQFMPSSFQRYAVDFDG
NGHTNLWDPVDAIGSVANYFKAHGWEQGQPVAVRGQGQLPGYEHGFQTRYPITTLHRAGLNPISNLGNHSEASLLRLDVG
TGYQYWYGLPNFYTITRYNHSTHYAMAVWQLGLAVKAARGGY
>Mature_362_residues
MRRLVSVLTLSLFPLLYGCSSTPAPKPVAKPFKSAFINQTPQIKPAYSQGVSKGDFANRNNVDSFVQRMVEKHGFDSVEL
HRLLAQADRYDWIIRLMDAQAPTTAKPIGPTGAWNRYRAKFITPDNVQNGVNFWREYESELNRAEQEYGVPPEIIVGIIG
VETRWGRVMGKTRILDALATLAFDYPRRAEFFTGELEAFLVMTRDEGMDPTEPKGSYAGAMGYGQFMPSSFQRYAVDFDG
NGHTNLWDPVDAIGSVANYFKAHGWEQGQPVAVRGQGQLPGYEHGFQTRYPITTLHRAGLNPISNLGNHSEASLLRLDVG
TGYQYWYGLPNFYTITRYNHSTHYAMAVWQLGLAVKAARGGY

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=364, Percent_Identity=66.4835164835165, Blast_Score=478, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 40608; Mature: 40608

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLVSVLTLSLFPLLYGCSSTPAPKPVAKPFKSAFINQTPQIKPAYSQGVSKGDFANRN
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCC
NVDSFVQRMVEKHGFDSVELHRLLAQADRYDWIIRLMDAQAPTTAKPIGPTGAWNRYRAK
CHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
FITPDNVQNGVNFWREYESELNRAEQEYGVPPEIIVGIIGVETRWGRVMGKTRILDALAT
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHH
LAFDYPRRAEFFTGELEAFLVMTRDEGMDPTEPKGSYAGAMGYGQFMPSSFQRYAVDFDG
HHHCCCCCCHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHCCCCCHHEEEEEECC
NGHTNLWDPVDAIGSVANYFKAHGWEQGQPVAVRGQGQLPGYEHGFQTRYPITTLHRAGL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHCCC
NPISNLGNHSEASLLRLDVGTGYQYWYGLPNFYTITRYNHSTHYAMAVWQLGLAVKAARG
CHHHHCCCCCCCEEEEEECCCCCCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCC
GY
CC
>Mature Secondary Structure
MRRLVSVLTLSLFPLLYGCSSTPAPKPVAKPFKSAFINQTPQIKPAYSQGVSKGDFANRN
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCC
NVDSFVQRMVEKHGFDSVELHRLLAQADRYDWIIRLMDAQAPTTAKPIGPTGAWNRYRAK
CHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
FITPDNVQNGVNFWREYESELNRAEQEYGVPPEIIVGIIGVETRWGRVMGKTRILDALAT
EECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHH
LAFDYPRRAEFFTGELEAFLVMTRDEGMDPTEPKGSYAGAMGYGQFMPSSFQRYAVDFDG
HHHCCCCCCHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHCCCCCHHEEEEEECC
NGHTNLWDPVDAIGSVANYFKAHGWEQGQPVAVRGQGQLPGYEHGFQTRYPITTLHRAGL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHCCC
NPISNLGNHSEASLLRLDVGTGYQYWYGLPNFYTITRYNHSTHYAMAVWQLGLAVKAARG
CHHHHCCCCCCCEEEEEECCCCCCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCC
GY
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]