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The map label for this gene is btuB [H]

Identifier: 145299621

GI number: 145299621

Start: 2888659

End: 2890797

Strand: Direct

Name: btuB [H]

Synonym: ASA_2695

Alternate gene names: 145299621

Gene position: 2888659-2890797 (Clockwise)

Preceding gene: 145299620

Following gene: 145299622

Centisome position: 61.43

GC content: 63.3

Gene sequence:

>2139_bases
ATGCTGCTGTCCGGCACAGCGCTGGCCGCCAGCGATCCGGCCAGGGTCGACGAAACTGGCATACTTCCTCACCTAAAGGC
GCGCGAGACCATAGTGGTCAAGGGCCAGCGGGTCGAACAGAAGCTGTCCGATATCTCGGGCGCCATCACAGTCATCACCG
AAGAGGACCTGGATCGCCAGGTCGCCACCGAACTCACCGACGTGTTCAAGAACGAACCCGGCGTTTCTGTGACCGGCTCG
GCAGGGCGTCCGCAAAACATCAGCATCCGCGGCATTGGCGGCAACCGGGTGCTGATCATCAAGGACGGGGTCAGGGTCAG
CGACGGCTTCGGTGCCGACGATCTCAACGACAAGGTGGGCCGTTTCAGCTTCGATCTGGATGACGTCAAGCAGATTGAGG
TGGCCAAGGGGGCCGGCTCCGCCCTGCACGGATCAGACGCCATCGGCGGCACCATCGTCATCTCCACCAAGCAGCCGGAG
GATTATCTGCAGGGGCAGGATGCCTACCTCAGCAGCAAAGTCCTGCATGATGGCAGCAGCAACAAGCAGAAGTTGAGCGC
CACCGGTGCCGCCCGCTGGGCTGAGACAGAACACCTGCTGCGCGTCTCTGGCTGGCAGGGGCACGAGACCGCCAACTATG
ACGAGAGCCGCATCCCGGCGGATCTGGATGGCCTGAGTGCCTCCACCAGCTCTCGCTTCACCCTGAACGAGCACCACCTG
CTGCGCCTCGAACTCGACTACTTCGAGGACAATGCCAAGCGGGATCAGGGGCCGCGCGAAATGCCCCAGCCCGATGGCAA
ATGGCAGGTTCGCCAGTATCGCGAGGACAGCACCCAGCGCTCCCAGGGCGCCAAGCTGAGCTGGGAGGCGCGCAGCCTCG
ACCATCTGCTGGCGGATCAGTTCACCTTGAGCCTGCACGGCAATCACGCCAAGAACAGCGCCAACAAGCGCAGCCTGCTG
CAAAATGACCTGCTGAGCGGCTATCCCCGCTATCGCAGCGAACGGGAGCTGGCCACCTTCACCGAGGATCTGCTCGGCGG
CGAGCTGGACATTCGCCGCGATCTGACGACCGGCGCCATCGAACACAAGCTGAGCTATGGCATCGAACTGGAGCGTGCAA
GCCATGAGCGACCGGTCGACAAACTGACCTTGGAGGCGGGCATTCCCCAGCCCGGCCAATCCGCCCCCTTCAGCAATGCC
CGCACGGACCGCGCCGGCATCTGGCTGGGGGATGTGGCGAGCCTTGGCCAGTGGCAATTCACCCCCACACTGCGCATGGA
TCACCAGTGGCTGAGCCCGCAAGATGGCAAGGGTGCCAGCAACCACAGCTGGCACCTCTCCCCCAGTCTGGCCACCAGCT
ACCGCTTCAACGATCAGTGGCGCAGCTGGCTCAGCTATGCCAACGGTTTTAGTGCCCCCTCCTACGACAAGGTGTACGGC
AATATTCCCCACTACTTTGCCCTGCCGCCGTTCGAGATCATCGCCAACCCGGACCTCAAGGAGGAGACCAGCCACAGCTT
CGAGTGGGGTGTGAGCGGTGAAGGGGATGAGTGGAGCATCAAGCCGGCGCTCTTCTACAACCGCTACTACAACTTCGTCG
ACTGGCGCCAGGTGGGCTTCCGTATGAGCGACGGCGTCATGCTGCGCCAATACCGCAACGTGGCCAAGTCCGAGACCTGG
GGCGCCGAGATCGCCGCCAGCTACTGGCTGACCCAGCAGTGGGAACTGGCCACCAAGTTCGGCTGGGTGGATGGCAAGGA
CAGCCAGGGCGAGTCACCGCGCAACCTGACCCCGCTGGAGGGCAATACCCGCCTCAGCTATCAGGCCAACGACTGGAGCC
TGGGGCTGCAAGCCGATTATGCCGCCGCCATGAGCCGGGTACCTACTTGCGGCAACAGCCTGACCCAGCAGCAGAGCGAG
TGCATGAAGAGTGCCGGCTGGCTCAGCTTCGCCCTGACCGCCGACTGGCAGATCACAGACGCACTCAAGGCCAACCTCAA
GCTAGATAACCTGCTCGATACCCGTTACACCCGCTATCAGGATGTAGCCGGGCAGGAAGCGGGCAGCGATGCGGGACTCT
ACACCCAGACGGGCCGCTACCTGAGCGCCAGCCTGCGCTACACCTTCGTGGGGATCTGA

Upstream 100 bases:

>100_bases
TCAATCGTTGATCTGGATGGACCACTCTGCAATAATCCGCGCCCTTCAGGCTCAAATAACAATCATTCTCAAAATGAAAA
TACTGCTGCACTACTGGCCC

Downstream 100 bases:

>100_bases
TGCACGCGCTATTGATAATGACGGCTCTGCTGGCCAATCCGGTGGCCTCCCCCGCCGAGGCCGCAATGACGGCAACCACC
ACGGCGCCACAGAGCGCGCC

Product: hemin receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 712; Mature: 712

Protein sequence:

>712_residues
MLLSGTALAASDPARVDETGILPHLKARETIVVKGQRVEQKLSDISGAITVITEEDLDRQVATELTDVFKNEPGVSVTGS
AGRPQNISIRGIGGNRVLIIKDGVRVSDGFGADDLNDKVGRFSFDLDDVKQIEVAKGAGSALHGSDAIGGTIVISTKQPE
DYLQGQDAYLSSKVLHDGSSNKQKLSATGAARWAETEHLLRVSGWQGHETANYDESRIPADLDGLSASTSSRFTLNEHHL
LRLELDYFEDNAKRDQGPREMPQPDGKWQVRQYREDSTQRSQGAKLSWEARSLDHLLADQFTLSLHGNHAKNSANKRSLL
QNDLLSGYPRYRSERELATFTEDLLGGELDIRRDLTTGAIEHKLSYGIELERASHERPVDKLTLEAGIPQPGQSAPFSNA
RTDRAGIWLGDVASLGQWQFTPTLRMDHQWLSPQDGKGASNHSWHLSPSLATSYRFNDQWRSWLSYANGFSAPSYDKVYG
NIPHYFALPPFEIIANPDLKEETSHSFEWGVSGEGDEWSIKPALFYNRYYNFVDWRQVGFRMSDGVMLRQYRNVAKSETW
GAEIAASYWLTQQWELATKFGWVDGKDSQGESPRNLTPLEGNTRLSYQANDWSLGLQADYAAAMSRVPTCGNSLTQQQSE
CMKSAGWLSFALTADWQITDALKANLKLDNLLDTRYTRYQDVAGQEAGSDAGLYTQTGRYLSASLRYTFVGI

Sequences:

>Translated_712_residues
MLLSGTALAASDPARVDETGILPHLKARETIVVKGQRVEQKLSDISGAITVITEEDLDRQVATELTDVFKNEPGVSVTGS
AGRPQNISIRGIGGNRVLIIKDGVRVSDGFGADDLNDKVGRFSFDLDDVKQIEVAKGAGSALHGSDAIGGTIVISTKQPE
DYLQGQDAYLSSKVLHDGSSNKQKLSATGAARWAETEHLLRVSGWQGHETANYDESRIPADLDGLSASTSSRFTLNEHHL
LRLELDYFEDNAKRDQGPREMPQPDGKWQVRQYREDSTQRSQGAKLSWEARSLDHLLADQFTLSLHGNHAKNSANKRSLL
QNDLLSGYPRYRSERELATFTEDLLGGELDIRRDLTTGAIEHKLSYGIELERASHERPVDKLTLEAGIPQPGQSAPFSNA
RTDRAGIWLGDVASLGQWQFTPTLRMDHQWLSPQDGKGASNHSWHLSPSLATSYRFNDQWRSWLSYANGFSAPSYDKVYG
NIPHYFALPPFEIIANPDLKEETSHSFEWGVSGEGDEWSIKPALFYNRYYNFVDWRQVGFRMSDGVMLRQYRNVAKSETW
GAEIAASYWLTQQWELATKFGWVDGKDSQGESPRNLTPLEGNTRLSYQANDWSLGLQADYAAAMSRVPTCGNSLTQQQSE
CMKSAGWLSFALTADWQITDALKANLKLDNLLDTRYTRYQDVAGQEAGSDAGLYTQTGRYLSASLRYTFVGI
>Mature_712_residues
MLLSGTALAASDPARVDETGILPHLKARETIVVKGQRVEQKLSDISGAITVITEEDLDRQVATELTDVFKNEPGVSVTGS
AGRPQNISIRGIGGNRVLIIKDGVRVSDGFGADDLNDKVGRFSFDLDDVKQIEVAKGAGSALHGSDAIGGTIVISTKQPE
DYLQGQDAYLSSKVLHDGSSNKQKLSATGAARWAETEHLLRVSGWQGHETANYDESRIPADLDGLSASTSSRFTLNEHHL
LRLELDYFEDNAKRDQGPREMPQPDGKWQVRQYREDSTQRSQGAKLSWEARSLDHLLADQFTLSLHGNHAKNSANKRSLL
QNDLLSGYPRYRSERELATFTEDLLGGELDIRRDLTTGAIEHKLSYGIELERASHERPVDKLTLEAGIPQPGQSAPFSNA
RTDRAGIWLGDVASLGQWQFTPTLRMDHQWLSPQDGKGASNHSWHLSPSLATSYRFNDQWRSWLSYANGFSAPSYDKVYG
NIPHYFALPPFEIIANPDLKEETSHSFEWGVSGEGDEWSIKPALFYNRYYNFVDWRQVGFRMSDGVMLRQYRNVAKSETW
GAEIAASYWLTQQWELATKFGWVDGKDSQGESPRNLTPLEGNTRLSYQANDWSLGLQADYAAAMSRVPTCGNSLTQQQSE
CMKSAGWLSFALTADWQITDALKANLKLDNLLDTRYTRYQDVAGQEAGSDAGLYTQTGRYLSASLRYTFVGI

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG1629

COG function: function code P; Outer membrane receptor proteins, mostly Fe transport

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=135, Percent_Identity=41.4814814814815, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790405, Length=136, Percent_Identity=37.5, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1790743, Length=626, Percent_Identity=21.8849840255591, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 79208; Mature: 79208

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSGTALAASDPARVDETGILPHLKARETIVVKGQRVEQKLSDISGAITVITEEDLDRQ
CEECCCEECCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCEEEEEECHHHHHH
VATELTDVFKNEPGVSVTGSAGRPQNISIRGIGGNRVLIIKDGVRVSDGFGADDLNDKVG
HHHHHHHHHCCCCCCEEECCCCCCCEEEEEEECCCEEEEEECCEEECCCCCCCCCCCCCC
RFSFDLDDVKQIEVAKGAGSALHGSDAIGGTIVISTKQPEDYLQGQDAYLSSKVLHDGSS
EEEECHHHHHHEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCHHHHHHHHCCCCC
NKQKLSATGAARWAETEHLLRVSGWQGHETANYDESRIPADLDGLSASTSSRFTLNEHHL
CCCEEHHCCCHHHHCCHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEE
LRLELDYFEDNAKRDQGPREMPQPDGKWQVRQYREDSTQRSQGAKLSWEARSLDHLLADQ
EEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCC
FTLSLHGNHAKNSANKRSLLQNDLLSGYPRYRSERELATFTEDLLGGELDIRRDLTTGAI
EEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEHHCCHHHHH
EHKLSYGIELERASHERPVDKLTLEAGIPQPGQSAPFSNARTDRAGIWLGDVASLGQWQF
HHHHHCCEEEECCCCCCCCHHEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCEEE
TPTLRMDHQWLSPQDGKGASNHSWHLSPSLATSYRFNDQWRSWLSYANGFSAPSYDKVYG
CCEEEECCCCCCCCCCCCCCCCCEEECCCHHHCEEECHHHHHHHHHHCCCCCCCHHHHHC
NIPHYFALPPFEIIANPDLKEETSHSFEWGVSGEGDEWSIKPALFYNRYYNFVDWRQVGF
CCCCEECCCCHHEECCCCCCHHCCCCEECCCCCCCCCEEECHHHHHHHHCCCHHHHHHCE
RMSDGVMLRQYRNVAKSETWGAEIAASYWLTQQWELATKFGWVDGKDSQGESPRNLTPLE
ECCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEHHHHCCCCCCCCCCCCCCCCCCCCC
GNTRLSYQANDWSLGLQADYAAAMSRVPTCGNSLTQQQSECMKSAGWLSFALTADWQITD
CCCEEEEECCCEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCEEHH
ALKANLKLDNLLDTRYTRYQDVAGQEAGSDAGLYTQTGRYLSASLRYTFVGI
HHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCEEEEEEEEEEEEC
>Mature Secondary Structure
MLLSGTALAASDPARVDETGILPHLKARETIVVKGQRVEQKLSDISGAITVITEEDLDRQ
CEECCCEECCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCEEEEEECHHHHHH
VATELTDVFKNEPGVSVTGSAGRPQNISIRGIGGNRVLIIKDGVRVSDGFGADDLNDKVG
HHHHHHHHHCCCCCCEEECCCCCCCEEEEEEECCCEEEEEECCEEECCCCCCCCCCCCCC
RFSFDLDDVKQIEVAKGAGSALHGSDAIGGTIVISTKQPEDYLQGQDAYLSSKVLHDGSS
EEEECHHHHHHEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCHHHHHHHHCCCCC
NKQKLSATGAARWAETEHLLRVSGWQGHETANYDESRIPADLDGLSASTSSRFTLNEHHL
CCCEEHHCCCHHHHCCHHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEE
LRLELDYFEDNAKRDQGPREMPQPDGKWQVRQYREDSTQRSQGAKLSWEARSLDHLLADQ
EEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCC
FTLSLHGNHAKNSANKRSLLQNDLLSGYPRYRSERELATFTEDLLGGELDIRRDLTTGAI
EEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEHHCCHHHHH
EHKLSYGIELERASHERPVDKLTLEAGIPQPGQSAPFSNARTDRAGIWLGDVASLGQWQF
HHHHHCCEEEECCCCCCCCHHEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCEEE
TPTLRMDHQWLSPQDGKGASNHSWHLSPSLATSYRFNDQWRSWLSYANGFSAPSYDKVYG
CCEEEECCCCCCCCCCCCCCCCCEEECCCHHHCEEECHHHHHHHHHHCCCCCCCHHHHHC
NIPHYFALPPFEIIANPDLKEETSHSFEWGVSGEGDEWSIKPALFYNRYYNFVDWRQVGF
CCCCEECCCCHHEECCCCCCHHCCCCEECCCCCCCCCEEECHHHHHHHHCCCHHHHHHCE
RMSDGVMLRQYRNVAKSETWGAEIAASYWLTQQWELATKFGWVDGKDSQGESPRNLTPLE
ECCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEHHHHCCCCCCCCCCCCCCCCCCCCC
GNTRLSYQANDWSLGLQADYAAAMSRVPTCGNSLTQQQSECMKSAGWLSFALTADWQITD
CCCEEEEECCCEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCEEHH
ALKANLKLDNLLDTRYTRYQDVAGQEAGSDAGLYTQTGRYLSASLRYTFVGI
HHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]