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The map label for this gene is pflA [H]

Identifier: 145299597

GI number: 145299597

Start: 2864930

End: 2865715

Strand: Direct

Name: pflA [H]

Synonym: ASA_2671

Alternate gene names: 145299597

Gene position: 2864930-2865715 (Clockwise)

Preceding gene: 145299594

Following gene: 145299598

Centisome position: 60.92

GC content: 55.6

Gene sequence:

>786_bases
ATGTCTGTCAGTAATCAGATCCCTGCCGTTGAAACCAGTGAATCCGGTGTCATTGGCCGGATCCATTCCGTTGAAACCTG
CGGCACCGTTGATGGCCCCGGAATACGATTTATCGTATTCATGCAAGGCTGTCTGATGCGCTGCAAGTACTGCCACAATC
GGGATACCTGGGATACCCAGGGTGGCCGCGAAGTGACAGTGCCGGAGTTGATGAGCGACATCACCAGCTATCGCCACTTC
ATGAATGCCTCGGGTGGTGGTGTCACCGCCTCCGGTGGCGAAGCCATGCTGCAACAAAACTTTATCGCCGAACTGTTTGC
CGCCTGCAAGGAGAAGGGTGTTCACACCTGCCTCGATACCAATGGCTTTGTGCGTCACTACGACGAACAGCTCGACAAGG
TGCTCGACAACACGGATCTGGTGCTGCTCGACATCAAGCAGATCAATGACGAGAAGCACATCCCGCTGACCCACGTCAGC
AACAAGTACACCCTGGAGTTCGCCCGTTATCTCGCCGCCAAAGGCAAGACAATGTGGATCCGCTACGTGGTGGTGCCGAC
CTGGTCAGACGATGACGAGAGCGCAGAGGGACTGGGCCAGTTCATCGCCGAACTGGGGGATAGTGTCGAGAAGGTGGAAC
TGCTGCCCTATCACGAGCTGGGCAAGCACAAGTGGGACGTTCTGGGTGAAAACTATGATCTGACCGGGATCAAGCCGCCC
AGCAAGGAGACCATGGACAGAGTTCAGGGCATACTCGCCAAGTACCACAACAACGTCAAATACTGA

Upstream 100 bases:

>100_bases
TGGAGAGCCTGCTCGGCGCGGCCCAGTCGGGCTAAACAGGTAGGATTTATAGGGATTTATGTAATAAAATCACGGCCTGC
TCGAATCGTTGGAGTCGTCT

Downstream 100 bases:

>100_bases
TGCCTCATCAGGGCTGGCAAGGAATACGCCAGCCCTGATGATATTGATACTGCATCACTGCACGCCATCACATGACAAGG
AAATGCCATGAGTGAAGGAC

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSVSNQIPAVETSESGVIGRIHSVETCGTVDGPGIRFIVFMQGCLMRCKYCHNRDTWDTQGGREVTVPELMSDITSYRHF
MNASGGGVTASGGEAMLQQNFIAELFAACKEKGVHTCLDTNGFVRHYDEQLDKVLDNTDLVLLDIKQINDEKHIPLTHVS
NKYTLEFARYLAAKGKTMWIRYVVVPTWSDDDESAEGLGQFIAELGDSVEKVELLPYHELGKHKWDVLGENYDLTGIKPP
SKETMDRVQGILAKYHNNVKY

Sequences:

>Translated_261_residues
MSVSNQIPAVETSESGVIGRIHSVETCGTVDGPGIRFIVFMQGCLMRCKYCHNRDTWDTQGGREVTVPELMSDITSYRHF
MNASGGGVTASGGEAMLQQNFIAELFAACKEKGVHTCLDTNGFVRHYDEQLDKVLDNTDLVLLDIKQINDEKHIPLTHVS
NKYTLEFARYLAAKGKTMWIRYVVVPTWSDDDESAEGLGQFIAELGDSVEKVELLPYHELGKHKWDVLGENYDLTGIKPP
SKETMDRVQGILAKYHNNVKY
>Mature_260_residues
SVSNQIPAVETSESGVIGRIHSVETCGTVDGPGIRFIVFMQGCLMRCKYCHNRDTWDTQGGREVTVPELMSDITSYRHFM
NASGGGVTASGGEAMLQQNFIAELFAACKEKGVHTCLDTNGFVRHYDEQLDKVLDNTDLVLLDIKQINDEKHIPLTHVSN
KYTLEFARYLAAKGKTMWIRYVVVPTWSDDDESAEGLGQFIAELGDSVEKVELLPYHELGKHKWDVLGENYDLTGIKPPS
KETMDRVQGILAKYHNNVKY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=245, Percent_Identity=68.1632653061224, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1790389, Length=265, Percent_Identity=25.2830188679245, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1790839, Length=275, Percent_Identity=28.3636363636364, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI226510931, Length=287, Percent_Identity=26.1324041811847, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 29250; Mature: 29119

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSNQIPAVETSESGVIGRIHSVETCGTVDGPGIRFIVFMQGCLMRCKYCHNRDTWDTQ
CCCCCCCCCEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
GGREVTVPELMSDITSYRHFMNASGGGVTASGGEAMLQQNFIAELFAACKEKGVHTCLDT
CCCEEEHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECC
NGFVRHYDEQLDKVLDNTDLVLLDIKQINDEKHIPLTHVSNKYTLEFARYLAAKGKTMWI
CCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHCCCEEEE
RYVVVPTWSDDDESAEGLGQFIAELGDSVEKVELLPYHELGKHKWDVLGENYDLTGIKPP
EEEEEECCCCCCHHHHHHHHHHHHHCCCHHHEEECCHHHHCCCHHHHCCCCCCCCCCCCC
SKETMDRVQGILAKYHNNVKY
CHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SVSNQIPAVETSESGVIGRIHSVETCGTVDGPGIRFIVFMQGCLMRCKYCHNRDTWDTQ
CCCCCCCCEECCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
GGREVTVPELMSDITSYRHFMNASGGGVTASGGEAMLQQNFIAELFAACKEKGVHTCLDT
CCCEEEHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECC
NGFVRHYDEQLDKVLDNTDLVLLDIKQINDEKHIPLTHVSNKYTLEFARYLAAKGKTMWI
CCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHCCCEEEE
RYVVVPTWSDDDESAEGLGQFIAELGDSVEKVELLPYHELGKHKWDVLGENYDLTGIKPP
EEEEEECCCCCCHHHHHHHHHHHHHCCCHHHEEECCHHHHCCCHHHHCCCCCCCCCCCCC
SKETMDRVQGILAKYHNNVKY
CHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]