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The map label for this gene is ahpF [H]

Identifier: 145299559

GI number: 145299559

Start: 2808862

End: 2810454

Strand: Direct

Name: ahpF [H]

Synonym: ASA_2628

Alternate gene names: 145299559

Gene position: 2808862-2810454 (Clockwise)

Preceding gene: 145299558

Following gene: 145299561

Centisome position: 59.73

GC content: 60.26

Gene sequence:

>1593_bases
ATGTTGGATACCAACCTCAAACAACAGCTGAACACCTATCTGCAGTACATCGTCAATCCCATCGAGATCAGCGTGTCCGG
CAACGACAGTGACAAATCCGCAGAACTGCTGGCATTGGCCCGCGAGATCAGCGAGATGTCCCCCAAAATTGCTATGACTG
ATGGCAGTAATGCCCGCAAGCCTTCCATGAGCGTTGCACCACAGGGCCAAGCCCCGCGCGTTCATTTTGCCGGCATCCCC
ATGGGTCATGAATTTACCTCTCTGGTGCTCGCCTTGCTGCAAAGCGGCGGCCACCCTTCCAAGACAGATCCGGCCGTACT
GGAGCAGATCAAGCAGCTGAAGGGCGAGTTTCACTTCGAGACCTATATCTCCCTCTCCTGCCACAACTGCCCGGACGTGG
TACAGGCGCTGAACCTGATGGCGACCCTGAATCCCGGTATCACCCACACCATGATCGACGGAGCCCTGTTCCAGGACGAA
GTGAACGAGCGCCAGATCATGGCGGTGCCGAACGTCTACATGAACGGTCAGCCGTTCAGCCAGGGCCGTATCTCGCTGGA
AGAGATCATTGCCAAGCTCGATACCGGTGCGGCCGATCGCAAGGCCGCCGAACTGAGCGAAAAAGCCCCCTATGACGTGC
TGGTAGTGGGTGGTGGCCCGGCTGGTGCCGCAGCAGCCATCTATGCCGCCCGTAAAGGCATTCGTACCGCCATCGTCGCA
GAGCGTTTTGGTGGTCAGGTGATGGACACGGTCGGGATCGAGAACTTCATCTCCGTGCCCTACACCGAAGGTCCCAAGCT
GGCCGCAAGCCTGGAGCAGCACGTCAAGCAGTACGGTGTGGAAGTGATCACCGAGCAGCGTGCCGCCGCCATCAGCAAGG
ACGGCTATGTGAATGTGGAGCTGGCCTCTGGTGCCACCCTCAAGAGTCGCGCCGTGATCCTGGCGACCGGTGCCCGCTGG
CGTGACCTGAACGTGCCGGGCGAGCTCGAGTACCGCACCAAGGGCGTGGCCTACTGCCCCCACTGCGACGGTCCCTTCTT
CAAGGGCAAGCGCGTGGCAGTGATCGGTGGTGGTAACTCCGGTATCGAAGCAGCCATCGATCTCGCCGGCATCGTCGAGC
ACGTCACAGTGGTGGAATTTGCCGATACCCTGCGCGCCGACGAAGTGCTGCAGAAGAAAGCCCGCTCCATGGGCAACATC
GACATCATCATGAGCGCCCGCACCACGGAAGTGATCGGTGATGGCAGCAAGGTGGTGGGGATGGATTATGAAGATCGCAC
CACGGGTGAAATCAAGCACTTAGGCGTGGCCGGCATCTTCGTCCAGATCGGACTGGTGCCGAACACCGAGTTCCTCAAGG
GGAGCGAAGTGGCACTGACCCGCTTCGGCGAGATCGAGATCGACACCAAGGGCGCCACTTCCCTGCCCGGTGTCTATGCT
GCCGGTGACGCCACTACAGTGCCGTTCAAGCAGATCATCATCTCCATGGGAGCCGGTGCAACCGCAGCGCTGGGGGCATT
TGATTACCTCATCCGCAATGCGGCGCCAGAGGCGGCAGAAACATCCGCCAAAGCCGATGCCGTGACCGCCTGA

Upstream 100 bases:

>100_bases
CCAAGACCGGCCCATTCAAGGGCCGGTCTGCTTTCTGGCCTCCTCTTGGATCATCAGGCCAGTCATCGCCGACCCGCAAT
CATTAAGAAGGAATTCGAAG

Downstream 100 bases:

>100_bases
TAACGGCCCTCGTGCCAGTAAAAAGCCGACTCTCTGGAGTCGGCTTTTTTTATTCGTGTTATTCACCAAGCGCTAGTTGG
CCTTCACATCCTCCGCCTCG

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MLDTNLKQQLNTYLQYIVNPIEISVSGNDSDKSAELLALAREISEMSPKIAMTDGSNARKPSMSVAPQGQAPRVHFAGIP
MGHEFTSLVLALLQSGGHPSKTDPAVLEQIKQLKGEFHFETYISLSCHNCPDVVQALNLMATLNPGITHTMIDGALFQDE
VNERQIMAVPNVYMNGQPFSQGRISLEEIIAKLDTGAADRKAAELSEKAPYDVLVVGGGPAGAAAAIYAARKGIRTAIVA
ERFGGQVMDTVGIENFISVPYTEGPKLAASLEQHVKQYGVEVITEQRAAAISKDGYVNVELASGATLKSRAVILATGARW
RDLNVPGELEYRTKGVAYCPHCDGPFFKGKRVAVIGGGNSGIEAAIDLAGIVEHVTVVEFADTLRADEVLQKKARSMGNI
DIIMSARTTEVIGDGSKVVGMDYEDRTTGEIKHLGVAGIFVQIGLVPNTEFLKGSEVALTRFGEIEIDTKGATSLPGVYA
AGDATTVPFKQIIISMGAGATAALGAFDYLIRNAAPEAAETSAKADAVTA

Sequences:

>Translated_530_residues
MLDTNLKQQLNTYLQYIVNPIEISVSGNDSDKSAELLALAREISEMSPKIAMTDGSNARKPSMSVAPQGQAPRVHFAGIP
MGHEFTSLVLALLQSGGHPSKTDPAVLEQIKQLKGEFHFETYISLSCHNCPDVVQALNLMATLNPGITHTMIDGALFQDE
VNERQIMAVPNVYMNGQPFSQGRISLEEIIAKLDTGAADRKAAELSEKAPYDVLVVGGGPAGAAAAIYAARKGIRTAIVA
ERFGGQVMDTVGIENFISVPYTEGPKLAASLEQHVKQYGVEVITEQRAAAISKDGYVNVELASGATLKSRAVILATGARW
RDLNVPGELEYRTKGVAYCPHCDGPFFKGKRVAVIGGGNSGIEAAIDLAGIVEHVTVVEFADTLRADEVLQKKARSMGNI
DIIMSARTTEVIGDGSKVVGMDYEDRTTGEIKHLGVAGIFVQIGLVPNTEFLKGSEVALTRFGEIEIDTKGATSLPGVYA
AGDATTVPFKQIIISMGAGATAALGAFDYLIRNAAPEAAETSAKADAVTA
>Mature_530_residues
MLDTNLKQQLNTYLQYIVNPIEISVSGNDSDKSAELLALAREISEMSPKIAMTDGSNARKPSMSVAPQGQAPRVHFAGIP
MGHEFTSLVLALLQSGGHPSKTDPAVLEQIKQLKGEFHFETYISLSCHNCPDVVQALNLMATLNPGITHTMIDGALFQDE
VNERQIMAVPNVYMNGQPFSQGRISLEEIIAKLDTGAADRKAAELSEKAPYDVLVVGGGPAGAAAAIYAARKGIRTAIVA
ERFGGQVMDTVGIENFISVPYTEGPKLAASLEQHVKQYGVEVITEQRAAAISKDGYVNVELASGATLKSRAVILATGARW
RDLNVPGELEYRTKGVAYCPHCDGPFFKGKRVAVIGGGNSGIEAAIDLAGIVEHVTVVEFADTLRADEVLQKKARSMGNI
DIIMSARTTEVIGDGSKVVGMDYEDRTTGEIKHLGVAGIFVQIGLVPNTEFLKGSEVALTRFGEIEIDTKGATSLPGVYA
AGDATTVPFKQIIISMGAGATAALGAFDYLIRNAAPEAAETSAKADAVTA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=298, Percent_Identity=26.510067114094, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI226437568, Length=298, Percent_Identity=26.510067114094, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI65787454, Length=298, Percent_Identity=26.510067114094, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI87081763, Length=520, Percent_Identity=61.3461538461539, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=311, Percent_Identity=35.6913183279743, Blast_Score=159, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6320560, Length=311, Percent_Identity=34.7266881028939, Blast_Score=149, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6321898, Length=339, Percent_Identity=31.858407079646, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56177; Mature: 56177

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDTNLKQQLNTYLQYIVNPIEISVSGNDSDKSAELLALAREISEMSPKIAMTDGSNARK
CCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCC
PSMSVAPQGQAPRVHFAGIPMGHEFTSLVLALLQSGGHPSKTDPAVLEQIKQLKGEFHFE
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEE
TYISLSCHNCPDVVQALNLMATLNPGITHTMIDGALFQDEVNERQIMAVPNVYMNGQPFS
EEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHCCHHHHHCCCCCEEEECCCEEECCCCCC
QGRISLEEIIAKLDTGAADRKAAELSEKAPYDVLVVGGGPAGAAAAIYAARKGIRTAIVA
CCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCHHHHHHH
ERFGGQVMDTVGIENFISVPYTEGPKLAASLEQHVKQYGVEVITEQRAAAISKDGYVNVE
HHHCCHHHHHHCCHHHEECCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEE
LASGATLKSRAVILATGARWRDLNVPGELEYRTKGVAYCPHCDGPFFKGKRVAVIGGGNS
ECCCCCCCCCEEEEEECCCEEECCCCCCEEEECCCEEECCCCCCCCCCCCEEEEEECCCC
GIEAAIDLAGIVEHVTVVEFADTLRADEVLQKKARSMGNIDIIMSARTTEVIGDGSKVVG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCEEEE
MDYEDRTTGEIKHLGVAGIFVQIGLVPNTEFLKGSEVALTRFGEIEIDTKGATSLPGVYA
CCCCCCCCCCHHHCCEEEEEEEEEECCCCHHCCCCCEEEEECCEEEEECCCCCCCCCEEE
AGDATTVPFKQIIISMGAGATAALGAFDYLIRNAAPEAAETSAKADAVTA
CCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure
MLDTNLKQQLNTYLQYIVNPIEISVSGNDSDKSAELLALAREISEMSPKIAMTDGSNARK
CCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCC
PSMSVAPQGQAPRVHFAGIPMGHEFTSLVLALLQSGGHPSKTDPAVLEQIKQLKGEFHFE
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEE
TYISLSCHNCPDVVQALNLMATLNPGITHTMIDGALFQDEVNERQIMAVPNVYMNGQPFS
EEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHCCHHHHHCCCCCEEEECCCEEECCCCCC
QGRISLEEIIAKLDTGAADRKAAELSEKAPYDVLVVGGGPAGAAAAIYAARKGIRTAIVA
CCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCHHHHHHH
ERFGGQVMDTVGIENFISVPYTEGPKLAASLEQHVKQYGVEVITEQRAAAISKDGYVNVE
HHHCCHHHHHHCCHHHEECCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEE
LASGATLKSRAVILATGARWRDLNVPGELEYRTKGVAYCPHCDGPFFKGKRVAVIGGGNS
ECCCCCCCCCEEEEEECCCEEECCCCCCEEEECCCEEECCCCCCCCCCCCEEEEEECCCC
GIEAAIDLAGIVEHVTVVEFADTLRADEVLQKKARSMGNIDIIMSARTTEVIGDGSKVVG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCEEEE
MDYEDRTTGEIKHLGVAGIFVQIGLVPNTEFLKGSEVALTRFGEIEIDTKGATSLPGVYA
CCCCCCCCCCHHHCCEEEEEEEEEECCCCHHCCCCCEEEEECCEEEEECCCCCCCCCEEE
AGDATTVPFKQIIISMGAGATAALGAFDYLIRNAAPEAAETSAKADAVTA
CCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9190810 [H]