Click here to switch to the map view.

The map label for this gene is cspD [H]

Identifier: 145299398

GI number: 145299398

Start: 2612005

End: 2612223

Strand: Direct

Name: cspD [H]

Synonym: ASA_2448

Alternate gene names: 145299398

Gene position: 2612005-2612223 (Clockwise)

Preceding gene: 145299395

Following gene: 145299417

Centisome position: 55.55

GC content: 48.4

Gene sequence:

>219_bases
ATGGCAACCGGAACAGTCAAGTGGTTTAACAACGCAAAAGGATTTGGGTTTATCTGTCCGGAAGGGGGTGGTGAAGATAT
CTTCGCCCACTACTCCACGATACAAATGGAAGGTTACAAGACCTTAAAAGCAGGTCAGGCAGTCAACTTCGAACTGCAGC
AGGGGCCGAAAGGAAATCACGCCTCTGTCATCGTGCCAAACGAAGCACAGAACATGTAA

Upstream 100 bases:

>100_bases
TAATCGGCGCCCGTCTGCTATAGACGTATGCTACTAGTTATAAAGGGCTGGCGCAGGTTACTCAACGACTTCAGGGTAAT
CAATAAAAGGATGTAGATGT

Downstream 100 bases:

>100_bases
GAGTCAAATTGGATAAATAAAAAACCCGGCGAACCGCAAGGTTGCCGGGTTTTTTATTGTGACCTTTGAGTGCGAGCGTG
AAGCCAATCAGATGCAGGCT

Product: cold shock domain-containing protein CspD

Products: NA

Alternate protein names: CSP-D [H]

Number of amino acids: Translated: 72; Mature: 71

Protein sequence:

>72_residues
MATGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMEGYKTLKAGQAVNFELQQGPKGNHASVIVPNEAQNM

Sequences:

>Translated_72_residues
MATGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMEGYKTLKAGQAVNFELQQGPKGNHASVIVPNEAQNM
>Mature_71_residues
ATGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMEGYKTLKAGQAVNFELQQGPKGNHASVIVPNEAQNM

Specific function: Inhibits DNA replication at both initiation and elongation steps, most probably by binding to the opened, single- stranded regions at replication forks. Plays a regulatory role in chromosomal replication in nutrient-depleted cells [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=70, Percent_Identity=82.8571428571429, Blast_Score=128, Evalue=7e-32,
Organism=Escherichia coli, GI1786841, Length=62, Percent_Identity=54.8387096774194, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1789979, Length=59, Percent_Identity=55.9322033898305, Blast_Score=75, Evalue=7e-16,
Organism=Escherichia coli, GI1788126, Length=58, Percent_Identity=58.6206896551724, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1787839, Length=66, Percent_Identity=48.4848484848485, Blast_Score=72, Evalue=5e-15,
Organism=Escherichia coli, GI1787834, Length=63, Percent_Identity=52.3809523809524, Blast_Score=72, Evalue=8e-15,
Organism=Escherichia coli, GI2367114, Length=63, Percent_Identity=47.6190476190476, Blast_Score=71, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24658520, Length=61, Percent_Identity=44.2622950819672, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7778; Mature: 7647

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMEGYKTLKAGQAVNFELQQGPKGNH
CCCCEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCCEEECCCCEEEEEECCCCCCCC
ASVIVPNEAQNM
CEEEECCCCCCC
>Mature Secondary Structure 
ATGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMEGYKTLKAGQAVNFELQQGPKGNH
CCCEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCCEEECCCCEEEEEECCCCCCCC
ASVIVPNEAQNM
CEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]