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The map label for this gene is araD [H]

Identifier: 145299351

GI number: 145299351

Start: 2559191

End: 2559889

Strand: Direct

Name: araD [H]

Synonym: ASA_2398

Alternate gene names: 145299351

Gene position: 2559191-2559889 (Clockwise)

Preceding gene: 145299350

Following gene: 145299352

Centisome position: 54.42

GC content: 61.8

Gene sequence:

>699_bases
ATGAACCTCAAATCTCTCAAAGAGCAGGTGCTGACCGCCAACCTGGATCTGCCGCGCCATCATCTGGTGACCTTCACCTG
GGGCAATGTGAGCGGCATCGACAGGGAGCGGGGTCTGGTGGTCATCAAACCGAGCGGCGTCAGTTACGACAAGATGCAGC
TTGAGGACCTGGTGGTGCTGGATCTCGCGGGCAAGCAGGTGGAAGGATCCTTGCGCCCCTCTTCCGACACTGCCACCCAC
CTGGTGCTCTATCGCACTTACCCTGAACTTGGCGGCGTGGTGCACACCCACTCCACCCATGCCACCGCCTGGGCCCAGGC
GGGGCGCGCCATCCCGATTTTTGGCACCACCCAGGCCGACTATTTCAACGGCGAGATCCCCTGCTCCCGTCTGCTCACCC
CGAGGGAGGTCGAAGAGGATTACGAGGAGCTGACCGGTCATCTCATCGTCGAGACCCTCAAACAGACCCCCATTCTCACC
ATGCCGGGGGTGCTGGTGGCCAACCACGGCCCCTTCAGCTGGGGCAAGGATGCGGATGATGCGGTGCACAACGCCGTTGT
GCTGGAAGAGGTGGCGCGCATGGCCTGGCTCACCGGCCAGATAAACCCGCAGGCGCAAGCCCTGCCAGACTACCTGCTGG
AGAAACACTACCAGCGCAAACATGGCAAGCACGCCTATTACGGCCAGACCGAGACTTAA

Upstream 100 bases:

>100_bases
AGCCACACCCTGAACGGGTCGCCGAATATGAGCGGCTCTATCAGCGTTACCTTCAATGGAGCCATGCCGCCGAGCCCCTC
TACAACCAGGGAGCACTGGC

Downstream 100 bases:

>100_bases
TCAATCAAGAGGACAAGATCATGGAGTTTATGAAACAGCTGGAAGTGTGGTTTCTGGTCGGCAGCCAGCACCTGTATAGC
GCAACCACATTGAAGCAGGT

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: L-ascorbate utilization protein F; Phosphoribulose isomerase [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MNLKSLKEQVLTANLDLPRHHLVTFTWGNVSGIDRERGLVVIKPSGVSYDKMQLEDLVVLDLAGKQVEGSLRPSSDTATH
LVLYRTYPELGGVVHTHSTHATAWAQAGRAIPIFGTTQADYFNGEIPCSRLLTPREVEEDYEELTGHLIVETLKQTPILT
MPGVLVANHGPFSWGKDADDAVHNAVVLEEVARMAWLTGQINPQAQALPDYLLEKHYQRKHGKHAYYGQTET

Sequences:

>Translated_232_residues
MNLKSLKEQVLTANLDLPRHHLVTFTWGNVSGIDRERGLVVIKPSGVSYDKMQLEDLVVLDLAGKQVEGSLRPSSDTATH
LVLYRTYPELGGVVHTHSTHATAWAQAGRAIPIFGTTQADYFNGEIPCSRLLTPREVEEDYEELTGHLIVETLKQTPILT
MPGVLVANHGPFSWGKDADDAVHNAVVLEEVARMAWLTGQINPQAQALPDYLLEKHYQRKHGKHAYYGQTET
>Mature_232_residues
MNLKSLKEQVLTANLDLPRHHLVTFTWGNVSGIDRERGLVVIKPSGVSYDKMQLEDLVVLDLAGKQVEGSLRPSSDTATH
LVLYRTYPELGGVVHTHSTHATAWAQAGRAIPIFGTTQADYFNGEIPCSRLLTPREVEEDYEELTGHLIVETLKQTPILT
MPGVLVANHGPFSWGKDADDAVHNAVVLEEVARMAWLTGQINPQAQALPDYLLEKHYQRKHGKHAYYGQTET

Specific function: Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. Is involved in the anaerobic L-ascorbate utilization [H]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790642, Length=227, Percent_Identity=65.1982378854625, Blast_Score=318, Evalue=2e-88,
Organism=Escherichia coli, GI1786247, Length=230, Percent_Identity=59.1304347826087, Blast_Score=278, Evalue=1e-76,
Organism=Escherichia coli, GI1790008, Length=231, Percent_Identity=59.3073593073593, Blast_Score=276, Evalue=7e-76,
Organism=Escherichia coli, GI1789164, Length=141, Percent_Identity=34.0425531914894, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: =5.1.3.4 [H]

Molecular weight: Translated: 25800; Mature: 25800

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKSLKEQVLTANLDLPRHHLVTFTWGNVSGIDRERGLVVIKPSGVSYDKMQLEDLVVL
CCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCCCCHHHEECCEEEEE
DLAGKQVEGSLRPSSDTATHLVLYRTYPELGGVVHTHSTHATAWAQAGRAIPIFGTTQAD
EECCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCHHHHHHCCCEEEEEECCCCC
YFNGEIPCSRLLTPREVEEDYEELTGHLIVETLKQTPILTMPGVLVANHGPFSWGKDADD
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCCCCCHHH
AVHNAVVLEEVARMAWLTGQINPQAQALPDYLLEKHYQRKHGKHAYYGQTET
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNLKSLKEQVLTANLDLPRHHLVTFTWGNVSGIDRERGLVVIKPSGVSYDKMQLEDLVVL
CCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCEEEECCCCCCHHHEECCEEEEE
DLAGKQVEGSLRPSSDTATHLVLYRTYPELGGVVHTHSTHATAWAQAGRAIPIFGTTQAD
EECCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCHHHHHHCCCEEEEEECCCCC
YFNGEIPCSRLLTPREVEEDYEELTGHLIVETLKQTPILTMPGVLVANHGPFSWGKDADD
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEECCCCCCCCCCHHH
AVHNAVVLEEVARMAWLTGQINPQAQALPDYLLEKHYQRKHGKHAYYGQTET
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA