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The map label for this gene is norM [H]

Identifier: 145299157

GI number: 145299157

Start: 2317061

End: 2318413

Strand: Direct

Name: norM [H]

Synonym: ASA_2185

Alternate gene names: 145299157

Gene position: 2317061-2318413 (Clockwise)

Preceding gene: 145299156

Following gene: 145299158

Centisome position: 49.27

GC content: 61.05

Gene sequence:

>1353_bases
GTGCAACGTTATTGGTCCGAGGCGAGGCAGCTCGTTCGTTTAGCCAGCCCCATTCTGGTCGCCCAGGTGGCCCAGATCGC
CATGAACTTTGTCGATACCGTGATGGCGGGGCAAGTCAGCGCCGTGGATCTGGCGGCCGTGTCGGTTGCCACCAGCTTCT
GGTTGCCGATCATATTGCTGGTGCAGGGCATCATAATGGCGCTCACCCCCATCGTCTCCCAGCTCAACGGGGCTCGCAAA
CGGGACGAGATCCGCCCGGCCGTGCATCAGGGCTTCTGGCTGGCGCTCATGGTCACTCCCATCGCCATGATAGCGCTCTA
TTTCAGCCCGCTGACCCTGCAATTCATGGGGGTAGACACTGTCATGGCCACCAAGACTGCCGGCTATCTGCATGCCATTT
TGTGGGGGCTGCCCGCCTTCGTGCTGTTCCAGGTACTGCGCAACTTCAGTGAGGGACTCTCCCACACCATGCCCACCATG
GTGATAGGTTTCGTCGGGCTGGCGGTCAATATTCCCGCCAACTACATCTTCATCCACGGCCATTTTGGCATGCCAAAACT
CGGCGGTGTCGGTTGTGGGGTGGCCACCGCCATCGTGCTGTGGGCCATGCTGCTGGCAATGATCATCTATGTGAAACTCT
CCCCTCACTTCACGAAGATCAACCTGTTTGCCCAGCTGGCACGCCCCAATGGCAGCCGGATCTGGCGCCTGTTCCGACTC
GGCTTTCCCATTGCCATGGCCATCTTCTGCGAGGTGACCCTGTTTACCGTGGTAGCCCTGATGCTGGCCCCGTTCGGAGC
AGAAACGGTCGCAAGCCATCAGATAGCGCTGAACTTCTCCAGCCTGGTGTTCATGCTGCCGCTCTCCATTGGTGTGGGGG
TGACTATCCGGGTCGGCCACAGCATAGGGGAAGGCTTGCCGGACCACGCCCGGGTCGCGGCCCGTACCGGCCTCATGCTG
GGCATGGCGGTCGCGGCCTGCACCGCCGCCCTGACAGTGCTGCTGCGTGAACAGATAGCAACCATCTACACAGATGACAT
GCAGGTGATCACGCTGGCAGCTACCTTGCTGTTCTTCGCCGCCATCTATCAGGTCTCCGACTCGGTACAGGTGGTAGCGG
CCGCGGCGCTGCGTGGATACAAGGACACCAAGGCGATTTTCTACGTCACCATAGTCGCCTACTGGGGTCTGGGCCTGCCT
ACCGGCATGCTGCTGGGACTGACCGATTGGGTCGTACCTCGCATGGGGCCGCAGGGCTTCTGGGTCGGTTTTATCGTCGG
CCTGACCGGTGCAGCCCTGATGCTGGGCGCCCGTCTCAAGGTGATCTATGGCCGTTTCGGCGCCCATCCGTAA

Upstream 100 bases:

>100_bases
AGAATGACACCCCCGCATTTTGTCTCCTCCCGGTGAGGGGGCAAAAGCCCATCCTGTTATCCGTTCTTGCTCTTTTTATC
TGATCCACTCGGAGGTGTCA

Downstream 100 bases:

>100_bases
CGGACTGATCTGGCTGTGGGGGATGCAGCTGGCAGGCTGTACCCCCACCATAGAGGATCAATTTGCCACCTATCAAACCA
GGGTGGCCCATGTACTGAGT

Product: multidrug resistance protein NorM

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter; Na(+)/drug antiporter [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MQRYWSEARQLVRLASPILVAQVAQIAMNFVDTVMAGQVSAVDLAAVSVATSFWLPIILLVQGIIMALTPIVSQLNGARK
RDEIRPAVHQGFWLALMVTPIAMIALYFSPLTLQFMGVDTVMATKTAGYLHAILWGLPAFVLFQVLRNFSEGLSHTMPTM
VIGFVGLAVNIPANYIFIHGHFGMPKLGGVGCGVATAIVLWAMLLAMIIYVKLSPHFTKINLFAQLARPNGSRIWRLFRL
GFPIAMAIFCEVTLFTVVALMLAPFGAETVASHQIALNFSSLVFMLPLSIGVGVTIRVGHSIGEGLPDHARVAARTGLML
GMAVAACTAALTVLLREQIATIYTDDMQVITLAATLLFFAAIYQVSDSVQVVAAAALRGYKDTKAIFYVTIVAYWGLGLP
TGMLLGLTDWVVPRMGPQGFWVGFIVGLTGAALMLGARLKVIYGRFGAHP

Sequences:

>Translated_450_residues
MQRYWSEARQLVRLASPILVAQVAQIAMNFVDTVMAGQVSAVDLAAVSVATSFWLPIILLVQGIIMALTPIVSQLNGARK
RDEIRPAVHQGFWLALMVTPIAMIALYFSPLTLQFMGVDTVMATKTAGYLHAILWGLPAFVLFQVLRNFSEGLSHTMPTM
VIGFVGLAVNIPANYIFIHGHFGMPKLGGVGCGVATAIVLWAMLLAMIIYVKLSPHFTKINLFAQLARPNGSRIWRLFRL
GFPIAMAIFCEVTLFTVVALMLAPFGAETVASHQIALNFSSLVFMLPLSIGVGVTIRVGHSIGEGLPDHARVAARTGLML
GMAVAACTAALTVLLREQIATIYTDDMQVITLAATLLFFAAIYQVSDSVQVVAAAALRGYKDTKAIFYVTIVAYWGLGLP
TGMLLGLTDWVVPRMGPQGFWVGFIVGLTGAALMLGARLKVIYGRFGAHP
>Mature_450_residues
MQRYWSEARQLVRLASPILVAQVAQIAMNFVDTVMAGQVSAVDLAAVSVATSFWLPIILLVQGIIMALTPIVSQLNGARK
RDEIRPAVHQGFWLALMVTPIAMIALYFSPLTLQFMGVDTVMATKTAGYLHAILWGLPAFVLFQVLRNFSEGLSHTMPTM
VIGFVGLAVNIPANYIFIHGHFGMPKLGGVGCGVATAIVLWAMLLAMIIYVKLSPHFTKINLFAQLARPNGSRIWRLFRL
GFPIAMAIFCEVTLFTVVALMLAPFGAETVASHQIALNFSSLVFMLPLSIGVGVTIRVGHSIGEGLPDHARVAARTGLML
GMAVAACTAALTVLLREQIATIYTDDMQVITLAATLLFFAAIYQVSDSVQVVAAAALRGYKDTKAIFYVTIVAYWGLGLP
TGMLLGLTDWVVPRMGPQGFWVGFIVGLTGAALMLGARLKVIYGRFGAHP

Specific function: Multidrug efflux pump that functions as a Na(+)/drug antiporter. Confers resistance to several drugs, such as norfloxacin, ciprofloxacin, ethidium, kanamycin and streptomycin [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI153792564, Length=425, Percent_Identity=23.7647058823529, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI38261960, Length=459, Percent_Identity=22.2222222222222, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI22907060, Length=434, Percent_Identity=23.0414746543779, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI48994890, Length=441, Percent_Identity=55.7823129251701, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI226510954, Length=409, Percent_Identity=24.4498777506112, Blast_Score=92, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320545, Length=434, Percent_Identity=23.5023041474654, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321821, Length=331, Percent_Identity=22.6586102719033, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 48554; Mature: 48554

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9661020; 12620739; 9987140; 11073914 [H]