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The map label for this gene is purT [H]

Identifier: 145299153

GI number: 145299153

Start: 2313813

End: 2314991

Strand: Direct

Name: purT [H]

Synonym: ASA_2181

Alternate gene names: 145299153

Gene position: 2313813-2314991 (Clockwise)

Preceding gene: 145299152

Following gene: 145299154

Centisome position: 49.2

GC content: 64.8

Gene sequence:

>1179_bases
ATGTTTGGAACCGCCACCCGCCCCAGCGCCACCCGCGCCTTGCTGCTTGGCTCGGGAGAACTCGGCAAGGAAGTCGCCAT
CGAGTTGCAACGGTTCGGGATCGAGGTGATCGCCGCCGACCGTTACCCCAATGCCCCCGCCATGCAGGTGGCACACAAAG
CCCACGTGCTGGACATGCTCGATGGCAATGCTCTGCGCGCCCTGGTCACCCTGGTCAAACCCGATCTCATCATCCCTGAA
ATCGAGGCCATCGCCACCGATACCCTCGCACAGCTTGAGCAAGAGGGGGTCAAGGTGGTGCCGAACGCCCGCGCCACCCA
GCTCACCATGAATCGGGAAGGGATCCGCCGGCTCGCCGCCGAAGAGTTGGGTCTGCCCACCTCCCCCTACCGTTTTGCCC
AGAGCAAGGAGGAGTTCATCGCCGCCGTCGAAGCCATCGGCCTGCCCTGCGTGGTAAAACCCGTGATGAGCTCGTCGGGC
AAGGGGCAGAGCGTGCTGCGGGATCTCGCCAAACTGGACGAGAGCTGGACTTACGCCCAGGAGGGTGGCCGTGCCGGTCG
CGGCAAGGTGATTGTTGAAGGATTCGTCCCCTTCGAGTACGAGATCACCCTGCTCACGGTGCGGGCCGTCGATGGCATTC
ATTTTTGCGACCCCATAGGCCACCGCCAGGAAGATGGCGACTACCGCGAATCCTGGCAGCCACAAGCGATGAGCACCCTG
GCGCTGGCCCGCTCTAAGGAGGTCGCCGCCAAGGTGGTCGGGGCGCTGGGCGGTTATGGCCTGTTCGGGGTCGAGCTCTT
TATCAGGGGCGATGAGGTGTGGTTCAGCGAAGTCTCCCCCCGCCCCCACGACACTGGCATGGTGACCCTGATCTCTCAGG
ATCTCTCCGAATTTGCCCTCCATGTGCGCGCCATCCTGGGGCTGCCCATCGGCACCATCACCCAGTACGGGCCGAGCGCC
TCGGCCGTGGTGCTGCGGGATGGTCACAGTCAGGACATCCGCTATCAGGGCATTGGAGCGGCGCTGGCCTTGGTATCGGG
CGCCCAGCTGCGGTTGTTTGGCAAGCCCGAGATCGCCGGTCGCCGCCGGCTCGGGGTCGCCCTGGCCCGTGGTCAGCAGT
GTGAAGAGGCGGTGGAAAAAGCCAAGGCGGTCGCCGCCCGGGTCGAGGTGATTTGTTGA

Upstream 100 bases:

>100_bases
ATCTGTTTCCTGAGGTACGCCATGCGTGCCTCTTTTTTTGCCTGCTATTGCTGCCAATAGCGACAGAGACATTGTGACCA
TCGACATCAGGAGAGATCGT

Downstream 100 bases:

>100_bases
CCAAAACCGGTTGCTGACGTGATCAGGCGCAAGGGGCATGGCAATGCCCCTTTTGCATTTTCCCGTCAGGCTTATAATCC
CCCTTCAAATTTGTCAGGAG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MFGTATRPSATRALLLGSGELGKEVAIELQRFGIEVIAADRYPNAPAMQVAHKAHVLDMLDGNALRALVTLVKPDLIIPE
IEAIATDTLAQLEQEGVKVVPNARATQLTMNREGIRRLAAEELGLPTSPYRFAQSKEEFIAAVEAIGLPCVVKPVMSSSG
KGQSVLRDLAKLDESWTYAQEGGRAGRGKVIVEGFVPFEYEITLLTVRAVDGIHFCDPIGHRQEDGDYRESWQPQAMSTL
ALARSKEVAAKVVGALGGYGLFGVELFIRGDEVWFSEVSPRPHDTGMVTLISQDLSEFALHVRAILGLPIGTITQYGPSA
SAVVLRDGHSQDIRYQGIGAALALVSGAQLRLFGKPEIAGRRRLGVALARGQQCEEAVEKAKAVAARVEVIC

Sequences:

>Translated_392_residues
MFGTATRPSATRALLLGSGELGKEVAIELQRFGIEVIAADRYPNAPAMQVAHKAHVLDMLDGNALRALVTLVKPDLIIPE
IEAIATDTLAQLEQEGVKVVPNARATQLTMNREGIRRLAAEELGLPTSPYRFAQSKEEFIAAVEAIGLPCVVKPVMSSSG
KGQSVLRDLAKLDESWTYAQEGGRAGRGKVIVEGFVPFEYEITLLTVRAVDGIHFCDPIGHRQEDGDYRESWQPQAMSTL
ALARSKEVAAKVVGALGGYGLFGVELFIRGDEVWFSEVSPRPHDTGMVTLISQDLSEFALHVRAILGLPIGTITQYGPSA
SAVVLRDGHSQDIRYQGIGAALALVSGAQLRLFGKPEIAGRRRLGVALARGQQCEEAVEKAKAVAARVEVIC
>Mature_392_residues
MFGTATRPSATRALLLGSGELGKEVAIELQRFGIEVIAADRYPNAPAMQVAHKAHVLDMLDGNALRALVTLVKPDLIIPE
IEAIATDTLAQLEQEGVKVVPNARATQLTMNREGIRRLAAEELGLPTSPYRFAQSKEEFIAAVEAIGLPCVVKPVMSSSG
KGQSVLRDLAKLDESWTYAQEGGRAGRGKVIVEGFVPFEYEITLLTVRAVDGIHFCDPIGHRQEDGDYRESWQPQAMSTL
ALARSKEVAAKVVGALGGYGLFGVELFIRGDEVWFSEVSPRPHDTGMVTLISQDLSEFALHVRAILGLPIGTITQYGPSA
SAVVLRDGHSQDIRYQGIGAALALVSGAQLRLFGKPEIAGRRRLGVALARGQQCEEAVEKAKAVAARVEVIC

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=390, Percent_Identity=69.2307692307692, Blast_Score=546, Evalue=1e-156,
Organism=Escherichia coli, GI1786733, Length=244, Percent_Identity=26.6393442622951, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324702, Length=376, Percent_Identity=24.7340425531915, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42172; Mature: 42172

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGTATRPSATRALLLGSGELGKEVAIELQRFGIEVIAADRYPNAPAMQVAHKAHVLDML
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHC
DGNALRALVTLVKPDLIIPEIEAIATDTLAQLEQEGVKVVPNARATQLTMNREGIRRLAA
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCEEEEECHHHHHHHHH
EELGLPTSPYRFAQSKEEFIAAVEAIGLPCVVKPVMSSSGKGQSVLRDLAKLDESWTYAQ
HHCCCCCCCHHHHCCHHHHHHHHHHHCCCCEEHHHHCCCCCCHHHHHHHHHHCCCCCHHH
EGGRAGRGKVIVEGFVPFEYEITLLTVRAVDGIHFCDPIGHRQEDGDYRESWQPQAMSTL
CCCCCCCCCEEEEECCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCHHHHHH
ALARSKEVAAKVVGALGGYGLFGVELFIRGDEVWFSEVSPRPHDTGMVTLISQDLSEFAL
HHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEHHHHHHHHHH
HVRAILGLPIGTITQYGPSASAVVLRDGHSQDIRYQGIGAALALVSGAQLRLFGKPEIAG
HHHHHHCCCHHHHHCCCCCCCEEEEECCCCCCCEECCHHHHHHHHCCCEEEEECCCCCCC
RRRLGVALARGQQCEEAVEKAKAVAARVEVIC
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFGTATRPSATRALLLGSGELGKEVAIELQRFGIEVIAADRYPNAPAMQVAHKAHVLDML
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHC
DGNALRALVTLVKPDLIIPEIEAIATDTLAQLEQEGVKVVPNARATQLTMNREGIRRLAA
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCEEEEECHHHHHHHHH
EELGLPTSPYRFAQSKEEFIAAVEAIGLPCVVKPVMSSSGKGQSVLRDLAKLDESWTYAQ
HHCCCCCCCHHHHCCHHHHHHHHHHHCCCCEEHHHHCCCCCCHHHHHHHHHHCCCCCHHH
EGGRAGRGKVIVEGFVPFEYEITLLTVRAVDGIHFCDPIGHRQEDGDYRESWQPQAMSTL
CCCCCCCCCEEEEECCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCHHHHHH
ALARSKEVAAKVVGALGGYGLFGVELFIRGDEVWFSEVSPRPHDTGMVTLISQDLSEFAL
HHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEHHHHHHHHHH
HVRAILGLPIGTITQYGPSASAVVLRDGHSQDIRYQGIGAALALVSGAQLRLFGKPEIAG
HHHHHHCCCHHHHHCCCCCCCEEEEECCCCCCCEECCHHHHHHHHCCCEEEEECCCCCCC
RRRLGVALARGQQCEEAVEKAKAVAARVEVIC
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA