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The map label for this gene is pdxK [H]

Identifier: 145297072

GI number: 145297072

Start: 3292169

End: 3292969

Strand: Reverse

Name: pdxK [H]

Synonym: cgR_2968

Alternate gene names: 145297072

Gene position: 3292969-3292169 (Counterclockwise)

Preceding gene: 145297075

Following gene: 145297071

Centisome position: 99.36

GC content: 56.18

Gene sequence:

>801_bases
GTGGTTAATTACGCATTCGTTATTGCAGGTTCAGAAGCTACCGGCGGCGCCGGCATCCAGGTTGATTTGAAGACCTTCCA
GCATTTAGATGTTTATGGCATTGGTGCCATCACATGCTTGGTGGCCTTTGATCCTAAAGACAACTGGAATCACCGTTTTG
TCCCGGTTGATGCACAGGTTATCGCTAACCAGATTGAGGCTGCCACCGCAGCGCACGATCTTGATGTGGTGAAGATTGGC
ATGCTGGGCACCCCAGTCACCATCGATACCGTGGCGAATGCCCTCCAGGAGAACAGCTTTAAGCACGTCGTCCTAGACCC
AGTACTGATCTGTAAGGGTCAGGAACCTGGCGCGGCACTTGATACCGACACCGCACTTCGCGCCAAGGTGCTGCCACAGG
CAACCGTGGTTACTCCAAACAACTTCGAGGCCACCACCCTGTCCGGCCTAGACAAGCTGGAGACCATCGACGACCTGAAG
GAAGCAGCCCGCCTCATTCACGAGCAGGGACCTCAGTACGTCGTTGTTAAGGGTGGCATCGACTTCCCAGGCGACAACGC
AGTGGACGTGCTTTTCGACGGCACCGACTACCACGTGTTCTCTGAACCAAAGATCGGCGACGAGCGCGTCTCCGGTGCTG
GCTGCACCTTCGCAGCTGTCATCACCGCAGAACTGGCCAAGGGCAACTCTGTCGTTGATGCAGTGACCACCGCTAAGCGC
GTAGTCACCCGTGCAGTGAAGGACGCTGTCGCATCCAACGCACCGTTTACCTCTGTGTGGTTGGCGGAAGACAACAAGTA
G

Upstream 100 bases:

>100_bases
GTGCGCACGATGATCGTTTTCGACATGCCCTTTAGCCTACGGATGAAATTTTTCAGACGGTAATGTCGGAATCTTCCACC
TGAGGGTCTAGGTTTGAAGT

Downstream 100 bases:

>100_bases
TTTGCTTGAATTTAGGATTTCCCTTGCGAAGTTGAGTTGGTAATTTCAACTTCGCAAGGGAATTTTGAACTTCAAATGAA
ATAGAGCAAGTGCTGTAATT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MVNYAFVIAGSEATGGAGIQVDLKTFQHLDVYGIGAITCLVAFDPKDNWNHRFVPVDAQVIANQIEAATAAHDLDVVKIG
MLGTPVTIDTVANALQENSFKHVVLDPVLICKGQEPGAALDTDTALRAKVLPQATVVTPNNFEATTLSGLDKLETIDDLK
EAARLIHEQGPQYVVVKGGIDFPGDNAVDVLFDGTDYHVFSEPKIGDERVSGAGCTFAAVITAELAKGNSVVDAVTTAKR
VVTRAVKDAVASNAPFTSVWLAEDNK

Sequences:

>Translated_266_residues
MVNYAFVIAGSEATGGAGIQVDLKTFQHLDVYGIGAITCLVAFDPKDNWNHRFVPVDAQVIANQIEAATAAHDLDVVKIG
MLGTPVTIDTVANALQENSFKHVVLDPVLICKGQEPGAALDTDTALRAKVLPQATVVTPNNFEATTLSGLDKLETIDDLK
EAARLIHEQGPQYVVVKGGIDFPGDNAVDVLFDGTDYHVFSEPKIGDERVSGAGCTFAAVITAELAKGNSVVDAVTTAKR
VVTRAVKDAVASNAPFTSVWLAEDNK
>Mature_266_residues
MVNYAFVIAGSEATGGAGIQVDLKTFQHLDVYGIGAITCLVAFDPKDNWNHRFVPVDAQVIANQIEAATAAHDLDVVKIG
MLGTPVTIDTVANALQENSFKHVVLDPVLICKGQEPGAALDTDTALRAKVLPQATVVTPNNFEATTLSGLDKLETIDDLK
EAARLIHEQGPQYVVVKGGIDFPGDNAVDVLFDGTDYHVFSEPKIGDERVSGAGCTFAAVITAELAKGNSVVDAVTTAKR
VVTRAVKDAVASNAPFTSVWLAEDNK

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates. Can also use hydroxymethylpyrimidine (HMP) as substrate [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=245, Percent_Identity=32.2448979591837, Blast_Score=123, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325378, Length=254, Percent_Identity=28.740157480315, Blast_Score=82, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324997, Length=273, Percent_Identity=30.4029304029304, Blast_Score=75, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324517, Length=275, Percent_Identity=30.1818181818182, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 28129; Mature: 28129

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNYAFVIAGSEATGGAGIQVDLKTFQHLDVYGIGAITCLVAFDPKDNWNHRFVPVDAQV
CCEEEEEEECCCCCCCCCEEEEEHHHCCCCEEECCEEEEEEEECCCCCCCEEEEECHHHH
IANQIEAATAAHDLDVVKIGMLGTPVTIDTVANALQENSFKHVVLDPVLICKGQEPGAAL
HHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHCCCCEEEECEEEEEECCCCCCCC
DTDTALRAKVLPQATVVTPNNFEATTLSGLDKLETIDDLKEAARLIHEQGPQYVVVKGGI
CCCHHHHHEECCCEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
DFPGDNAVDVLFDGTDYHVFSEPKIGDERVSGAGCTFAAVITAELAKGNSVVDAVTTAKR
CCCCCCEEEEEECCCCEEEECCCCCCHHHCCCCCCEEEEHHHHHHHCCCCHHHHHHHHHH
VVTRAVKDAVASNAPFTSVWLAEDNK
HHHHHHHHHHHCCCCCEEEEEECCCC
>Mature Secondary Structure
MVNYAFVIAGSEATGGAGIQVDLKTFQHLDVYGIGAITCLVAFDPKDNWNHRFVPVDAQV
CCEEEEEEECCCCCCCCCEEEEEHHHCCCCEEECCEEEEEEEECCCCCCCEEEEECHHHH
IANQIEAATAAHDLDVVKIGMLGTPVTIDTVANALQENSFKHVVLDPVLICKGQEPGAAL
HHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHCCCCEEEECEEEEEECCCCCCCC
DTDTALRAKVLPQATVVTPNNFEATTLSGLDKLETIDDLKEAARLIHEQGPQYVVVKGGI
CCCHHHHHEECCCEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
DFPGDNAVDVLFDGTDYHVFSEPKIGDERVSGAGCTFAAVITAELAKGNSVVDAVTTAKR
CCCCCCEEEEEECCCCEEEECCCCCCHHHCCCCCCEEEEHHHHHHHCCCCHHHHHHHHHH
VVTRAVKDAVASNAPFTSVWLAEDNK
HHHHHHHHHHHCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]