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The map label for this gene is budC [H]

Identifier: 145297040

GI number: 145297040

Start: 3255701

End: 3256456

Strand: Direct

Name: budC [H]

Synonym: cgR_2937

Alternate gene names: 145297040

Gene position: 3255701-3256456 (Clockwise)

Preceding gene: 145297035

Following gene: 145297041

Centisome position: 98.24

GC content: 55.16

Gene sequence:

>756_bases
ATGAAATCAATCTTCATTTCCGGTGCGGCGAACGGAATTGGCAAAGCTGTGGCGTTGAAATTTGTTCACGAAGGTTGGCT
CGTTGGAGCCTACGACATCGCGGAAATCACCTATTCACACCCCAATCTTCGCTGGGGCTACCTCAATGTTCGACAGTCCG
AGTCGTGGGACAAAGCCCTAGAAGACTTTGCGACGCACACCGGAGGCACCATCGATGTGGTGGACAATAATGCCGGCGTG
ATTATTGAGGGACCGCTGCAGGACGCAGAGGAGGGGAGCGTCGACAAGCTTCTTGCAATCAACGTCAATGGCGTGACTCT
TGGTGCCCGCGCCGCTCATCCTTATTTGGCGCGCACGCCGGGCGCCCAGTTGTTAAACATGTCCTCGGCATCGGCGGTGT
ACGGGCAGCCCCAGATCGCGGTGTATTCGGCTTCGAAGTTTTACGTTGCAGGTCTTACTGAGGCGCTGAATTTGGAGTGG
CGGAAAGACGATATTCGCGTGGTCGATGTTTGGCCTTTGTGGGTAAAAACCGATTTGGTGAACGGCGTGAAGGCTAAGTC
GCTGAAGCGTTTGGGCGTCCGGATCACTCCGGAACAGGTGGCACAGGCGGTATGGGATGCGGTGCATCCGAAATCTCGGT
GGGCGAAGGGAAAGGTGCATCACGGGGTGTCAAAGTTGGATAAGGCGCTGTATCTCATGAAATCTCTGTCGCCTGATCGG
GTAGCGATGTGTTTTGCGCGACTAATCGCCGGATAA

Upstream 100 bases:

>100_bases
AACCAACAAAGGTCATCTCAACCGGCTTAAGAAAATTCTGCCAGCTTTCTGCTGATTGAATCGTGCCAGCTCAGGGCATA
TCTCACCTAAAGTAAACACC

Downstream 100 bases:

>100_bases
ATGAATTGATTATTTTAGGCTCCCAGGGATTAAGTCTAGGGTGGAATGTAGAAATATTTCCTACGGAAGGTCCGTCATGA
CGCCTGCAGGTCCAGCACAA

Product: short chain dehydrogenase

Products: NA

Alternate protein names: Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKSIFISGAANGIGKAVALKFVHEGWLVGAYDIAEITYSHPNLRWGYLNVRQSESWDKALEDFATHTGGTIDVVDNNAGV
IIEGPLQDAEEGSVDKLLAINVNGVTLGARAAHPYLARTPGAQLLNMSSASAVYGQPQIAVYSASKFYVAGLTEALNLEW
RKDDIRVVDVWPLWVKTDLVNGVKAKSLKRLGVRITPEQVAQAVWDAVHPKSRWAKGKVHHGVSKLDKALYLMKSLSPDR
VAMCFARLIAG

Sequences:

>Translated_251_residues
MKSIFISGAANGIGKAVALKFVHEGWLVGAYDIAEITYSHPNLRWGYLNVRQSESWDKALEDFATHTGGTIDVVDNNAGV
IIEGPLQDAEEGSVDKLLAINVNGVTLGARAAHPYLARTPGAQLLNMSSASAVYGQPQIAVYSASKFYVAGLTEALNLEW
RKDDIRVVDVWPLWVKTDLVNGVKAKSLKRLGVRITPEQVAQAVWDAVHPKSRWAKGKVHHGVSKLDKALYLMKSLSPDR
VAMCFARLIAG
>Mature_251_residues
MKSIFISGAANGIGKAVALKFVHEGWLVGAYDIAEITYSHPNLRWGYLNVRQSESWDKALEDFATHTGGTIDVVDNNAGV
IIEGPLQDAEEGSVDKLLAINVNGVTLGARAAHPYLARTPGAQLLNMSSASAVYGQPQIAVYSASKFYVAGLTEALNLEW
RKDDIRVVDVWPLWVKTDLVNGVKAKSLKRLGVRITPEQVAQAVWDAVHPKSRWAKGKVHHGVSKLDKALYLMKSLSPDR
VAMCFARLIAG

Specific function: Catalyzes the reversible reduction of (S)-acetoin to 2,3-butanediol in the presence of NADH [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Escherichia coli, GI87082100, Length=223, Percent_Identity=27.8026905829596, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786701, Length=247, Percent_Identity=24.2914979757085, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1789208, Length=221, Percent_Identity=25.7918552036199, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71994600, Length=244, Percent_Identity=27.0491803278689, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=242, Percent_Identity=26.0330578512397, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281364892, Length=219, Percent_Identity=28.7671232876712, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24644339, Length=207, Percent_Identity=26.5700483091787, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI23397609, Length=188, Percent_Identity=29.7872340425532, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014007
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.304 [H]

Molecular weight: Translated: 27318; Mature: 27318

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIFISGAANGIGKAVALKFVHEGWLVGAYDIAEITYSHPNLRWGYLNVRQSESWDKAL
CCEEEEECCCCCHHHHHHHEEHHCCEEEEEEEEEEEEECCCCEEEEEEEECCCCCHHHHH
EDFATHTGGTIDVVDNNAGVIIEGPLQDAEEGSVDKLLAINVNGVTLGARAAHPYLARTP
HHHHHCCCCEEEEEECCCCEEEECCCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCC
GAQLLNMSSASAVYGQPQIAVYSASKFYVAGLTEALNLEWRKDDIRVVDVWPLWVKTDLV
CHHEEECCCCCEECCCCEEEEEECCEEEEEECHHHHCCEEECCCEEEEEEEEEEEEHHHH
NGVKAKSLKRLGVRITPEQVAQAVWDAVHPKSRWAKGKVHHGVSKLDKALYLMKSLSPDR
CCHHHHHHHHCCCEECHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCHHH
VAMCFARLIAG
HHHHHHHHHCC
>Mature Secondary Structure
MKSIFISGAANGIGKAVALKFVHEGWLVGAYDIAEITYSHPNLRWGYLNVRQSESWDKAL
CCEEEEECCCCCHHHHHHHEEHHCCEEEEEEEEEEEEECCCCEEEEEEEECCCCCHHHHH
EDFATHTGGTIDVVDNNAGVIIEGPLQDAEEGSVDKLLAINVNGVTLGARAAHPYLARTP
HHHHHCCCCEEEEEECCCCEEEECCCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCC
GAQLLNMSSASAVYGQPQIAVYSASKFYVAGLTEALNLEWRKDDIRVVDVWPLWVKTDLV
CHHEEECCCCCEECCCCEEEEEECCEEEEEECHHHHCCEEECCCEEEEEEEEEEEEHHHH
NGVKAKSLKRLGVRITPEQVAQAVWDAVHPKSRWAKGKVHHGVSKLDKALYLMKSLSPDR
CCHHHHHHHHCCCEECHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCHHH
VAMCFARLIAG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11173520 [H]