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The map label for this gene is dadA2 [H]

Identifier: 145297001

GI number: 145297001

Start: 3217322

End: 3218554

Strand: Reverse

Name: dadA2 [H]

Synonym: cgR_2900

Alternate gene names: 145297001

Gene position: 3218554-3217322 (Counterclockwise)

Preceding gene: 145297002

Following gene: 145296999

Centisome position: 97.11

GC content: 56.77

Gene sequence:

>1233_bases
ATGAGTTCGTCTGGAAAAGTCATTGTTGTTGGAGCCGGCATAGTGGGTCTTGCCACCGCCTGGCATTTACAGGAGCACGG
GTTCGAGGTGAGCGTCCTTGATCGGGATGGTGTCGCTGCAGGTTCTTCGTGGGGTAATGCTGGTTGGTTAGCGCCGGCGA
AAACTATTCCGTTGTCGGAGCCGGGGCTGTGGACGTATGGTCCGAAAGAGCTGTTCAATCCGGTGTCGCCGATGCATATG
CCACTTCGTGTGGATCCCAAACTGTGGCTTTTCTTGGCGCAATTTATGGCGCAGGCTTTTCAAAGCAAGTGGGATTCCAC
AATGGCGGATCTCACAGAGATCGATAAGGTCGCGCTCGAAGCTTTTGATGAGCTGTCGATCGGTGGCGTGGAAGGCCTCA
CCCATGAAGGTCCATTTGTTGTTGGTTTTGAGGAAGAGAGCCAATCGGCGGGTTTCCGTAAGGAAATTGATGGCGTGATC
AGGCACGGCCAGAAAGTGGAGATGTCTCGACTGGAGAATCCACGAGAGTTGGCGCCGATGCTGAATGAGCAAATTCAGGT
GGCTTACCGTTTGGAAGGCCAGCGTTTCATCGAGCCGGGTCCATACGTGCAGTCATTGGCGGATGCTGTGGTGAAGCGTG
GTGGCGTGATCCGCGCCGGGGCAGAAGTTGTGCATGTGGCGAAGGGTGATCGTCCCGCGGTCATTATGGCGGATGGTAGC
CGTGAAGAAGCGGACAAGGTGGTTGTCGCAACGGGTGCCTGGCTGCCGGGTCTAACGCGTGAATACGGTGTGAAAACTCT
TGTTCAGGCTGGTCGTGGCTATTCCTTCTCTGTTGCAACGGATATTCCTGCCAAGCATTCTGTGTACCTTCCCCACCACC
GCATGGCCTGCACGCCGTATGAGGGCCGTTTCCGCATTGCGGGCACCATGGAGTTCCGCGGTCCGGATGAGCCGTTCCAG
CAGGGCCGGGTGGATGCGATTGTTTCGCAGGCGAAGAAGGTTATGAGAGGTATTGATTTCAACGATCGCAAGGATGAGTG
GGTGGGTTCCCGCCCGGTCACTCCGGATGGGCGTCCGTTGATTGGGCAGACCAAGGCGGAGAACATTTACGTTGCCGGTG
GTCACGGCATGTGGGGTGTGGTGCTGGGCCCTGCCACCGGTAAGTATTTGGCGGAGCTGATGGCTACGGGCAACACCAAC
CCGATCATCAAGCCGTTCGATCCGCTGCGTTAA

Upstream 100 bases:

>100_bases
CAAGAAATAACCCCATAGGAATTTCCAAACCAACACATTTAAGTACGAACTCATGAATACTGGGGGCACTAGGAGTCCAC
CCCACCCTCAGGAGGGTTTC

Downstream 100 bases:

>100_bases
CTGCCCAATAATTGGTTGAAGATCTCCGAGGTTGCGGGATGGGTGTAGATGCCGTCGCCAAGCTCGGAGGCGGTGACGCC
ATGCCGCATGGCAAGCGCCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSSSGKVIVVGAGIVGLATAWHLQEHGFEVSVLDRDGVAAGSSWGNAGWLAPAKTIPLSEPGLWTYGPKELFNPVSPMHM
PLRVDPKLWLFLAQFMAQAFQSKWDSTMADLTEIDKVALEAFDELSIGGVEGLTHEGPFVVGFEEESQSAGFRKEIDGVI
RHGQKVEMSRLENPRELAPMLNEQIQVAYRLEGQRFIEPGPYVQSLADAVVKRGGVIRAGAEVVHVAKGDRPAVIMADGS
REEADKVVVATGAWLPGLTREYGVKTLVQAGRGYSFSVATDIPAKHSVYLPHHRMACTPYEGRFRIAGTMEFRGPDEPFQ
QGRVDAIVSQAKKVMRGIDFNDRKDEWVGSRPVTPDGRPLIGQTKAENIYVAGGHGMWGVVLGPATGKYLAELMATGNTN
PIIKPFDPLR

Sequences:

>Translated_410_residues
MSSSGKVIVVGAGIVGLATAWHLQEHGFEVSVLDRDGVAAGSSWGNAGWLAPAKTIPLSEPGLWTYGPKELFNPVSPMHM
PLRVDPKLWLFLAQFMAQAFQSKWDSTMADLTEIDKVALEAFDELSIGGVEGLTHEGPFVVGFEEESQSAGFRKEIDGVI
RHGQKVEMSRLENPRELAPMLNEQIQVAYRLEGQRFIEPGPYVQSLADAVVKRGGVIRAGAEVVHVAKGDRPAVIMADGS
REEADKVVVATGAWLPGLTREYGVKTLVQAGRGYSFSVATDIPAKHSVYLPHHRMACTPYEGRFRIAGTMEFRGPDEPFQ
QGRVDAIVSQAKKVMRGIDFNDRKDEWVGSRPVTPDGRPLIGQTKAENIYVAGGHGMWGVVLGPATGKYLAELMATGNTN
PIIKPFDPLR
>Mature_409_residues
SSSGKVIVVGAGIVGLATAWHLQEHGFEVSVLDRDGVAAGSSWGNAGWLAPAKTIPLSEPGLWTYGPKELFNPVSPMHMP
LRVDPKLWLFLAQFMAQAFQSKWDSTMADLTEIDKVALEAFDELSIGGVEGLTHEGPFVVGFEEESQSAGFRKEIDGVIR
HGQKVEMSRLENPRELAPMLNEQIQVAYRLEGQRFIEPGPYVQSLADAVVKRGGVIRAGAEVVHVAKGDRPAVIMADGSR
EEADKVVVATGAWLPGLTREYGVKTLVQAGRGYSFSVATDIPAKHSVYLPHHRMACTPYEGRFRIAGTMEFRGPDEPFQQ
GRVDAIVSQAKKVMRGIDFNDRKDEWVGSRPVTPDGRPLIGQTKAENIYVAGGHGMWGVVLGPATGKYLAELMATGNTNP
IIKPFDPLR

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=425, Percent_Identity=27.5294117647059, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 44626; Mature: 44495

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSGKVIVVGAGIVGLATAWHLQEHGFEVSVLDRDGVAAGSSWGNAGWLAPAKTIPLSE
CCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PGLWTYGPKELFNPVSPMHMPLRVDPKLWLFLAQFMAQAFQSKWDSTMADLTEIDKVALE
CCCCCCCHHHHCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFDELSIGGVEGLTHEGPFVVGFEEESQSAGFRKEIDGVIRHGQKVEMSRLENPRELAPM
HHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEHHHCCCHHHHHHH
LNEQIQVAYRLEGQRFIEPGPYVQSLADAVVKRGGVIRAGAEVVHVAKGDRPAVIMADGS
HHHHEEEEEEECCCEECCCCHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCEEEEECCC
REEADKVVVATGAWLPGLTREYGVKTLVQAGRGYSFSVATDIPAKHSVYLPHHRMACTPY
CCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEECCCCCEEECCC
EGRFRIAGTMEFRGPDEPFQQGRVDAIVSQAKKVMRGIDFNDRKDEWVGSRPVTPDGRPL
CCCEEEEEEEECCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
IGQTKAENIYVAGGHGMWGVVLGPATGKYLAELMATGNTNPIIKPFDPLR
CCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSSGKVIVVGAGIVGLATAWHLQEHGFEVSVLDRDGVAAGSSWGNAGWLAPAKTIPLSE
CCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
PGLWTYGPKELFNPVSPMHMPLRVDPKLWLFLAQFMAQAFQSKWDSTMADLTEIDKVALE
CCCCCCCHHHHCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFDELSIGGVEGLTHEGPFVVGFEEESQSAGFRKEIDGVIRHGQKVEMSRLENPRELAPM
HHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEHHHCCCHHHHHHH
LNEQIQVAYRLEGQRFIEPGPYVQSLADAVVKRGGVIRAGAEVVHVAKGDRPAVIMADGS
HHHHEEEEEEECCCEECCCCHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCEEEEECCC
REEADKVVVATGAWLPGLTREYGVKTLVQAGRGYSFSVATDIPAKHSVYLPHHRMACTPY
CCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEECCCCCEEECCC
EGRFRIAGTMEFRGPDEPFQQGRVDAIVSQAKKVMRGIDFNDRKDEWVGSRPVTPDGRPL
CCCEEEEEEEECCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
IGQTKAENIYVAGGHGMWGVVLGPATGKYLAELMATGNTNPIIKPFDPLR
CCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]