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The map label for this gene is maeB [C]

Identifier: 145296996

GI number: 145296996

Start: 3211694

End: 3212872

Strand: Direct

Name: maeB [C]

Synonym: cgR_2895

Alternate gene names: 145296996

Gene position: 3211694-3212872 (Clockwise)

Preceding gene: 145296995

Following gene: 145297000

Centisome position: 96.91

GC content: 58.52

Gene sequence:

>1179_bases
ATGACCATCGACCTGCAGCGTTCCACTCAAAACCTCACCTATGAGGAAATCTTCGAGGCTCACGAGGGCGGAAAGCTCTC
CATTAGTTCCACTCGTCCGCTCCGCGACATGCGTGATCTTTCCCTTGGTTACACCCCTGGTGTTGCTCAGGTTTGTGAAG
CAATCAAGGAAGATCCAGAGGTTGCACGCACCCACACGGGCATTGGAAACACCGTCGCGGTTATTTCCGACGGCACCGCT
GTTCTGGGTCTTGGCGATATCGGACCTCAGGCCTCCCTTCCCGTCATGGAGGGCAAGGCTCAGCTGTTTAGCTCTTTCGC
TGGCCTGAAGGCTATCCCTATCGTTTTGGATGTTCACGATGTTGACGCTTTGGTTGAGACCATCGCAGCCATCGCGCCTT
CTTTCGGTGCTATCAACTTGGAGGACATCTCCGCTCCTCGTTGCTTCGAGGTGGAGCGCCGCCTCATCGAGCGTCTTGAT
ATCCCAGTTATGCACGATGACCAGCACGGCACCGCTGTGGTTATCCTCGCTGCGCTGCGCAACTCCCTGAAGCTGCTGGA
TCGCAAGATCGAAGACCTCAAGATTGTTATTTCCGGCGCAGGTGCCGCTGGTGTTGCAGCTGTGGACATGCTGACCAACG
CTGGAGCAACCGATATTGTGGTTCTCGATTCCCGAGGCATCATCCACGACAGCCGTGAGGATCTTTCCCCAGTTAAGGAT
GCTCTTGCAGAGAAGACCAACCCTCGTGGCATCAGCGGTGGCATCAATGAGGCTTTCACCGGCGCGGACCTGTTCATTGG
CGTGTCCGGCGGCAACATCGGCGAGGACGCCCTCAAGCTCATGGCACCACAGCCAATCCTGTTCACCCTGGCGAACCCAA
CCCCAGAGATCGATCCTGAGCTGTCTCAGAAGTACGGCGCCATCGTCGCGACCGGCCGCTCTGACCTGCCTAACCAGATC
AACAACGTGCTCGCGTTCCCAGGAATTTTCGCCGGCGCTCTCGCAGCCAAGGCCAAGAAGATCACCCCCGAGATGAAGCT
CGCCGCTGCAGAGGCAATCGCCGACATCGCAGCTGAGGACCTCGAGGTCGGCCGCATCGTGCCTACCGCCCTGGATCCCC
GCGTCGCCCCAGCAGTCAAGGCAGCTGTCCAGGCCGTCGCCGAAGCGCAATACGCTTAA

Upstream 100 bases:

>100_bases
AGCTACATATTTAGCTAGTGTTTTTGTTCCAGAACCCTAAATGAGGTTCTACCCTTAACAGAGCTTCCCGCAAAAACACC
GATTAACAAGGCTAAATGAT

Downstream 100 bases:

>100_bases
GAATTTGCTTATCGACGCCTCCCTCCCCGTCGAGGCGCCAATATTTTAAGAGCAAACTTGAGGCCCACAGAAGCAATTCT
GTGGGCCTTCAAATTCGCTG

Product: hypothetical protein

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MTIDLQRSTQNLTYEEIFEAHEGGKLSISSTRPLRDMRDLSLGYTPGVAQVCEAIKEDPEVARTHTGIGNTVAVISDGTA
VLGLGDIGPQASLPVMEGKAQLFSSFAGLKAIPIVLDVHDVDALVETIAAIAPSFGAINLEDISAPRCFEVERRLIERLD
IPVMHDDQHGTAVVILAALRNSLKLLDRKIEDLKIVISGAGAAGVAAVDMLTNAGATDIVVLDSRGIIHDSREDLSPVKD
ALAEKTNPRGISGGINEAFTGADLFIGVSGGNIGEDALKLMAPQPILFTLANPTPEIDPELSQKYGAIVATGRSDLPNQI
NNVLAFPGIFAGALAAKAKKITPEMKLAAAEAIADIAAEDLEVGRIVPTALDPRVAPAVKAAVQAVAEAQYA

Sequences:

>Translated_392_residues
MTIDLQRSTQNLTYEEIFEAHEGGKLSISSTRPLRDMRDLSLGYTPGVAQVCEAIKEDPEVARTHTGIGNTVAVISDGTA
VLGLGDIGPQASLPVMEGKAQLFSSFAGLKAIPIVLDVHDVDALVETIAAIAPSFGAINLEDISAPRCFEVERRLIERLD
IPVMHDDQHGTAVVILAALRNSLKLLDRKIEDLKIVISGAGAAGVAAVDMLTNAGATDIVVLDSRGIIHDSREDLSPVKD
ALAEKTNPRGISGGINEAFTGADLFIGVSGGNIGEDALKLMAPQPILFTLANPTPEIDPELSQKYGAIVATGRSDLPNQI
NNVLAFPGIFAGALAAKAKKITPEMKLAAAEAIADIAAEDLEVGRIVPTALDPRVAPAVKAAVQAVAEAQYA
>Mature_391_residues
TIDLQRSTQNLTYEEIFEAHEGGKLSISSTRPLRDMRDLSLGYTPGVAQVCEAIKEDPEVARTHTGIGNTVAVISDGTAV
LGLGDIGPQASLPVMEGKAQLFSSFAGLKAIPIVLDVHDVDALVETIAAIAPSFGAINLEDISAPRCFEVERRLIERLDI
PVMHDDQHGTAVVILAALRNSLKLLDRKIEDLKIVISGAGAAGVAAVDMLTNAGATDIVVLDSRGIIHDSREDLSPVKDA
LAEKTNPRGISGGINEAFTGADLFIGVSGGNIGEDALKLMAPQPILFTLANPTPEIDPELSQKYGAIVATGRSDLPNQIN
NVLAFPGIFAGALAAKAKKITPEMKLAAAEAIADIAAEDLEVGRIVPTALDPRVAPAVKAAVQAVAEAQYA

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=358, Percent_Identity=27.9329608938547, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI62420882, Length=359, Percent_Identity=26.4623955431755, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI62420880, Length=359, Percent_Identity=26.4623955431755, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI239049447, Length=359, Percent_Identity=26.4623955431755, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI4505145, Length=360, Percent_Identity=26.3888888888889, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788806, Length=388, Percent_Identity=46.6494845360825, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI87081919, Length=479, Percent_Identity=24.6346555323591, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17537199, Length=432, Percent_Identity=26.8518518518519, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6322823, Length=347, Percent_Identity=26.5129682997118, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24646386, Length=358, Percent_Identity=27.3743016759777, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24646388, Length=358, Percent_Identity=27.6536312849162, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI281362672, Length=359, Percent_Identity=26.1838440111421, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI281362674, Length=359, Percent_Identity=26.1838440111421, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21356279, Length=359, Percent_Identity=26.1838440111421, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI78707232, Length=371, Percent_Identity=25.3369272237197, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281363503, Length=371, Percent_Identity=25.3369272237197, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281363505, Length=371, Percent_Identity=25.3369272237197, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI78707236, Length=371, Percent_Identity=25.3369272237197, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI78707240, Length=369, Percent_Identity=24.390243902439, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI78707242, Length=369, Percent_Identity=24.1192411924119, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI78707238, Length=369, Percent_Identity=24.1192411924119, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 41037; Mature: 40906

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDLQRSTQNLTYEEIFEAHEGGKLSISSTRPLRDMRDLSLGYTPGVAQVCEAIKEDPE
CEEECCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCCCCHHHHHHHHHCCHH
VARTHTGIGNTVAVISDGTAVLGLGDIGPQASLPVMEGKAQLFSSFAGLKAIPIVLDVHD
HHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEHH
VDALVETIAAIAPSFGAINLEDISAPRCFEVERRLIERLDIPVMHDDQHGTAVVILAALR
HHHHHHHHHHHCCCCCCEEHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHH
NSLKLLDRKIEDLKIVISGAGAAGVAAVDMLTNAGATDIVVLDSRGIIHDSREDLSPVKD
HHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHH
ALAEKTNPRGISGGINEAFTGADLFIGVSGGNIGEDALKLMAPQPILFTLANPTPEIDPE
HHHHCCCCCCCCCCCCHHCCCCEEEEEECCCCCCHHHHHHHCCCCEEEEECCCCCCCCHH
LSQKYGAIVATGRSDLPNQINNVLAFPGIFAGALAAKAKKITPEMKLAAAEAIADIAAED
HHHCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LEVGRIVPTALDPRVAPAVKAAVQAVAEAQYA
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIDLQRSTQNLTYEEIFEAHEGGKLSISSTRPLRDMRDLSLGYTPGVAQVCEAIKEDPE
EEECCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCCCCHHHHHHHHHCCHH
VARTHTGIGNTVAVISDGTAVLGLGDIGPQASLPVMEGKAQLFSSFAGLKAIPIVLDVHD
HHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEHH
VDALVETIAAIAPSFGAINLEDISAPRCFEVERRLIERLDIPVMHDDQHGTAVVILAALR
HHHHHHHHHHHCCCCCCEEHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHH
NSLKLLDRKIEDLKIVISGAGAAGVAAVDMLTNAGATDIVVLDSRGIIHDSREDLSPVKD
HHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHH
ALAEKTNPRGISGGINEAFTGADLFIGVSGGNIGEDALKLMAPQPILFTLANPTPEIDPE
HHHHCCCCCCCCCCCCHHCCCCEEEEEECCCCCCHHHHHHHCCCCEEEEECCCCCCCCHH
LSQKYGAIVATGRSDLPNQINNVLAFPGIFAGALAAKAKKITPEMKLAAAEAIADIAAED
HHHCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LEVGRIVPTALDPRVAPAVKAAVQAVAEAQYA
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]