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The map label for this gene is pbpG [H]

Identifier: 145296976

GI number: 145296976

Start: 3190305

End: 3192467

Strand: Reverse

Name: pbpG [H]

Synonym: cgR_2875

Alternate gene names: 145296976

Gene position: 3192467-3190305 (Counterclockwise)

Preceding gene: 145296977

Following gene: 145296975

Centisome position: 96.33

GC content: 53.26

Gene sequence:

>2163_bases
TTGACGAATAGTAAAAATCCTCCTGCCAAAAAGAGCAGCGGCAAAAGGCGCTTAAAGAGCGACTCTAAGGGCCACGCTGT
AAGAAACACCATCATTGGTGCCATTGTTGCTGTCATTTTGATTCCAGTAATGGTGTTCATGAGTGCTTACATCATGGTTG
ATGTTCCAGAACCGGAAGAGTTGGTTTCACCCCAGGTTTCGCAGATTTACGCATCTGACGGTGAGACTGAATTGGCACGC
ATCGTTCCTCCAGAAGGCAACCGCCAGATGGTGACGATCGATCAGGTGCCTGACACTGTGAAAAATGCGGTGGTGGCTGC
GGAAGACCGAGAGTTTTACACAAACCCCGGTTTTTCCATTACTGGCTATGCCCGAGCAGCACTTGGCGTAATCACTGGTG
ATTCTTCAGCGGGTGGTGGTTCCACCATTACTCAGCAGTATGTGAAGAAGGCTGTGGTTGGTGATGAGCGTTCGCTGATC
CGTAAGGCTAAGGAATTGGTCTATTCCGCGAAGATGGCCAATGAGTGGTCTAAGGACGAGGTCCTTGAGGCTTATCTCAA
CACTGTGTACTTCGGTCGAAATGCCTATGGTGTGCAGGCTGCAGCTCATGCATTCTTTGATAAGCCAGTAGAAGAGCTCA
CGGCTGCTGAGGGCGCAGTGCTGGCGGCCAGTATTCAGCTGCCAAGCCAGTTGGATCCTTGGACAAATCCAGTTGAGGCG
GAAACGCGTTGGAATTATGTCATGGACGGCCTGGTGGAAATCGGCGCTATCTCGGCAGAGGAGCGCGCAGTTGCTACCTA
CCCTGAAACCACTGACCCTGCGTCCAACAGTGCGTACACGGAAGCCACCGGCACCAATGGTTTGATCAAGAACCAAGTGA
TGGCTGAGTTGTCTGAGCTCGGAATCACTGAGGACGATGTGCAGACCCGTGGTTTGCAGGTCACCACCACTATTGATCCG
AAAACTCAGGAAGGTGCCGTTGAAGCGGTACAAAACCAGTTGGATCTTCTGTCTGAGAACAACCGTGCAGCGGTAGTCTC
CATTGATCCTTCTAATGGTGCGGTTCGTGCTTATTATGGCGGCGAGGATGCGACTGGTTGGGACTTTGCGAACGCCCCAA
TTCAGACTGGTTCCACGTTCAAGATTTTTGGTCTGGCGGCAGCTCTCCAGCAGGGTATCCCGCTGTCTCAGCCTTACAGC
TCTGCGCCGGTGACCGTGGGTGATGCTCAGATCGGAAACGTCGGTGGCAGCGGTTGTGGTTCCTGTTCCATCGAGCAGGC
GTTGTTGCATTCTTACAACACCAGCTTCATTCGTTTGCAGCAGGATCTGGAAAATGGTTCACAGGATACTGCAGACATGG
CGCATGCTTTGGGTATCGCGAAGTCTTTGCCAACTATCCCTGAGACACTGACTGAAAACGGAGAGACCCCTTATGAGGGC
ATCATCTTGGGTCAGTACGAGTCCCGCCCACTTGATATGGCTTCTGCGATGGCAACCATCGCTAATGAAGGCGTCTGGCA
CCGCCCGCACTTCGTGTCCAAGGTGGAGACTGTCAGCGGTGAGGTTCTCTACGAGTTCGAGGATGGCGACGGCGAGCGTC
GTGTTTCTGAAAAGGTTGCACTGAATCTGCTCAAGGCCATGGGGCCAATCGCTGCATACTCAAACGGAAACGCTCTGGCT
GATGGCCAGGTTTCTGCATCCAAGACTGGTACCACTCAGCTTGGTGATACCGGCGCAAACAAGGATGCGTGGATGTTGGG
TGCGGCACCTCAGCTGGCTACTGCGGTGTGGGTCGGTACTGCTGATAACACTGCGTTGTATAACACCTGGGGTGGCAGCA
TGTATGGTTCTAACTCTCCTGCCACGATCTGGAAGCAGACCATGGATAACGCCCTCGAGAACTCCCCTCTCGAAACTTGG
GATATCGCTCCAGCATTGGGGTACGGTAACCCACCAGTTCCGGAATATGTGTGGACTCCAAGTCCAAACATCGGGACTAA
TGATCCAGAAGTAGCAACCGAGGAAGCTCCAGTGGAGGATCCAAATGCAGTAATCGATACCCCTGCTGTAGATCCCACTG
CACCTGCAGAGGAGACCGGTAACGGTCAGGTAGAAATCCTGCCGGGGCTGACTATCCCGGGAGATCTCTTAGGGATCGGC
TAA

Upstream 100 bases:

>100_bases
GCCAGCTGATTCTCAGATTCAGATGAATAGGCTTAGGCGCAGTATTCTCTACAGCACAAGCTTCAGATAAGAACCAGATC
TGATTGAACGGAAACACCAG

Downstream 100 bases:

>100_bases
AATCCGGTCGTAGCCTAAACGACCTTAAAAAGGAGCCGACCGCCGATGGACCAGAAATTGGACCAGCAGAAGGTAGACCG
CGTATCGCCCGGTGATAGTG

Product: hypothetical protein

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MTNSKNPPAKKSSGKRRLKSDSKGHAVRNTIIGAIVAVILIPVMVFMSAYIMVDVPEPEELVSPQVSQIYASDGETELAR
IVPPEGNRQMVTIDQVPDTVKNAVVAAEDREFYTNPGFSITGYARAALGVITGDSSAGGGSTITQQYVKKAVVGDERSLI
RKAKELVYSAKMANEWSKDEVLEAYLNTVYFGRNAYGVQAAAHAFFDKPVEELTAAEGAVLAASIQLPSQLDPWTNPVEA
ETRWNYVMDGLVEIGAISAEERAVATYPETTDPASNSAYTEATGTNGLIKNQVMAELSELGITEDDVQTRGLQVTTTIDP
KTQEGAVEAVQNQLDLLSENNRAAVVSIDPSNGAVRAYYGGEDATGWDFANAPIQTGSTFKIFGLAAALQQGIPLSQPYS
SAPVTVGDAQIGNVGGSGCGSCSIEQALLHSYNTSFIRLQQDLENGSQDTADMAHALGIAKSLPTIPETLTENGETPYEG
IILGQYESRPLDMASAMATIANEGVWHRPHFVSKVETVSGEVLYEFEDGDGERRVSEKVALNLLKAMGPIAAYSNGNALA
DGQVSASKTGTTQLGDTGANKDAWMLGAAPQLATAVWVGTADNTALYNTWGGSMYGSNSPATIWKQTMDNALENSPLETW
DIAPALGYGNPPVPEYVWTPSPNIGTNDPEVATEEAPVEDPNAVIDTPAVDPTAPAEETGNGQVEILPGLTIPGDLLGIG

Sequences:

>Translated_720_residues
MTNSKNPPAKKSSGKRRLKSDSKGHAVRNTIIGAIVAVILIPVMVFMSAYIMVDVPEPEELVSPQVSQIYASDGETELAR
IVPPEGNRQMVTIDQVPDTVKNAVVAAEDREFYTNPGFSITGYARAALGVITGDSSAGGGSTITQQYVKKAVVGDERSLI
RKAKELVYSAKMANEWSKDEVLEAYLNTVYFGRNAYGVQAAAHAFFDKPVEELTAAEGAVLAASIQLPSQLDPWTNPVEA
ETRWNYVMDGLVEIGAISAEERAVATYPETTDPASNSAYTEATGTNGLIKNQVMAELSELGITEDDVQTRGLQVTTTIDP
KTQEGAVEAVQNQLDLLSENNRAAVVSIDPSNGAVRAYYGGEDATGWDFANAPIQTGSTFKIFGLAAALQQGIPLSQPYS
SAPVTVGDAQIGNVGGSGCGSCSIEQALLHSYNTSFIRLQQDLENGSQDTADMAHALGIAKSLPTIPETLTENGETPYEG
IILGQYESRPLDMASAMATIANEGVWHRPHFVSKVETVSGEVLYEFEDGDGERRVSEKVALNLLKAMGPIAAYSNGNALA
DGQVSASKTGTTQLGDTGANKDAWMLGAAPQLATAVWVGTADNTALYNTWGGSMYGSNSPATIWKQTMDNALENSPLETW
DIAPALGYGNPPVPEYVWTPSPNIGTNDPEVATEEAPVEDPNAVIDTPAVDPTAPAEETGNGQVEILPGLTIPGDLLGIG
>Mature_719_residues
TNSKNPPAKKSSGKRRLKSDSKGHAVRNTIIGAIVAVILIPVMVFMSAYIMVDVPEPEELVSPQVSQIYASDGETELARI
VPPEGNRQMVTIDQVPDTVKNAVVAAEDREFYTNPGFSITGYARAALGVITGDSSAGGGSTITQQYVKKAVVGDERSLIR
KAKELVYSAKMANEWSKDEVLEAYLNTVYFGRNAYGVQAAAHAFFDKPVEELTAAEGAVLAASIQLPSQLDPWTNPVEAE
TRWNYVMDGLVEIGAISAEERAVATYPETTDPASNSAYTEATGTNGLIKNQVMAELSELGITEDDVQTRGLQVTTTIDPK
TQEGAVEAVQNQLDLLSENNRAAVVSIDPSNGAVRAYYGGEDATGWDFANAPIQTGSTFKIFGLAAALQQGIPLSQPYSS
APVTVGDAQIGNVGGSGCGSCSIEQALLHSYNTSFIRLQQDLENGSQDTADMAHALGIAKSLPTIPETLTENGETPYEGI
ILGQYESRPLDMASAMATIANEGVWHRPHFVSKVETVSGEVLYEFEDGDGERRVSEKVALNLLKAMGPIAAYSNGNALAD
GQVSASKTGTTQLGDTGANKDAWMLGAAPQLATAVWVGTADNTALYNTWGGSMYGSNSPATIWKQTMDNALENSPLETWD
IAPALGYGNPPVPEYVWTPSPNIGTNDPEVATEEAPVEDPNAVIDTPAVDPTAPAEETGNGQVEILPGLTIPGDLLGIG

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=601, Percent_Identity=26.7886855241265, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87082258, Length=318, Percent_Identity=31.4465408805031, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1788867, Length=591, Percent_Identity=26.3959390862944, Blast_Score=136, Evalue=6e-33,
Organism=Escherichia coli, GI1789601, Length=165, Percent_Identity=32.1212121212121, Blast_Score=91, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 76200; Mature: 76069

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSKNPPAKKSSGKRRLKSDSKGHAVRNTIIGAIVAVILIPVMVFMSAYIMVDVPEPEE
CCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHH
LVSPQVSQIYASDGETELARIVPPEGNRQMVTIDQVPDTVKNAVVAAEDREFYTNPGFSI
HHCCHHHHHHHCCCCCCEEEECCCCCCCEEEEECCCCHHHHHCEEEECCCHHCCCCCCEE
TGYARAALGVITGDSSAGGGSTITQQYVKKAVVGDERSLIRKAKELVYSAKMANEWSKDE
EHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHH
VLEAYLNTVYFGRNAYGVQAAAHAFFDKPVEELTAAEGAVLAASIQLPSQLDPWTNPVEA
HHHHHHHHHEECCCCCCHHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
ETRWNYVMDGLVEIGAISAEERAVATYPETTDPASNSAYTEATGTNGLIKNQVMAELSEL
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHC
GITEDDVQTRGLQVTTTIDPKTQEGAVEAVQNQLDLLSENNRAAVVSIDPSNGAVRAYYG
CCCHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEC
GEDATGWDFANAPIQTGSTFKIFGLAAALQQGIPLSQPYSSAPVTVGDAQIGNVGGSGCG
CCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCEEECCCEECCCCCCCCC
SCSIEQALLHSYNTSFIRLQQDLENGSQDTADMAHALGIAKSLPTIPETLTENGETPYEG
CCHHHHHHHHHCCCCCEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCC
IILGQYESRPLDMASAMATIANEGVWHRPHFVSKVETVSGEVLYEFEDGDGERRVSEKVA
EEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHH
LNLLKAMGPIAAYSNGNALADGQVSASKTGTTQLGDTGANKDAWMLGAAPQLATAVWVGT
HHHHHHHCCEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHEEEEEEEC
ADNTALYNTWGGSMYGSNSPATIWKQTMDNALENSPLETWDIAPALGYGNPPVPEYVWTP
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECC
SPNIGTNDPEVATEEAPVEDPNAVIDTPAVDPTAPAEETGNGQVEILPGLTIPGDLLGIG
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCC
>Mature Secondary Structure 
TNSKNPPAKKSSGKRRLKSDSKGHAVRNTIIGAIVAVILIPVMVFMSAYIMVDVPEPEE
CCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHH
LVSPQVSQIYASDGETELARIVPPEGNRQMVTIDQVPDTVKNAVVAAEDREFYTNPGFSI
HHCCHHHHHHHCCCCCCEEEECCCCCCCEEEEECCCCHHHHHCEEEECCCHHCCCCCCEE
TGYARAALGVITGDSSAGGGSTITQQYVKKAVVGDERSLIRKAKELVYSAKMANEWSKDE
EHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHH
VLEAYLNTVYFGRNAYGVQAAAHAFFDKPVEELTAAEGAVLAASIQLPSQLDPWTNPVEA
HHHHHHHHHEECCCCCCHHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
ETRWNYVMDGLVEIGAISAEERAVATYPETTDPASNSAYTEATGTNGLIKNQVMAELSEL
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHC
GITEDDVQTRGLQVTTTIDPKTQEGAVEAVQNQLDLLSENNRAAVVSIDPSNGAVRAYYG
CCCHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEC
GEDATGWDFANAPIQTGSTFKIFGLAAALQQGIPLSQPYSSAPVTVGDAQIGNVGGSGCG
CCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCEEECCCEECCCCCCCCC
SCSIEQALLHSYNTSFIRLQQDLENGSQDTADMAHALGIAKSLPTIPETLTENGETPYEG
CCHHHHHHHHHCCCCCEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCC
IILGQYESRPLDMASAMATIANEGVWHRPHFVSKVETVSGEVLYEFEDGDGERRVSEKVA
EEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHH
LNLLKAMGPIAAYSNGNALADGQVSASKTGTTQLGDTGANKDAWMLGAAPQLATAVWVGT
HHHHHHHCCEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHEEEEEEEC
ADNTALYNTWGGSMYGSNSPATIWKQTMDNALENSPLETWDIAPALGYGNPPVPEYVWTP
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECC
SPNIGTNDPEVATEEAPVEDPNAVIDTPAVDPTAPAEETGNGQVEILPGLTIPGDLLGIG
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]