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The map label for this gene is dnaB [H]

Identifier: 145296969

GI number: 145296969

Start: 3184601

End: 3186133

Strand: Reverse

Name: dnaB [H]

Synonym: cgR_2868

Alternate gene names: 145296969

Gene position: 3186133-3184601 (Counterclockwise)

Preceding gene: 145296970

Following gene: 145296968

Centisome position: 96.14

GC content: 56.1

Gene sequence:

>1533_bases
ATGGCTACAGATACACACGCCGCAAGTTTTGATGATGACTACGTACCTGCACAGGAACCAAGCGATTCCTTCGCGGATGA
CGCACACGTAGATGTCCCTGCTCCGGCGTTTGAGGATTTCTCCCCGGCCCAGGCCTTTGGTCAGGGAACTAGGGGAGGAG
ACTCTCAGGGCTTCAAGAAGAGGGGCCGCAAGGACGAGTCCCGTGAATACCGAGACTTCCGTCAACCTCCCTATGACAAT
GATGCGGAGATGGGCGTGCTGGGCGCGATGCTGCTCAGCCCGACCACAGTCATCGACATTCTGGATATCCTCACCCCAGA
AGACTTCTATAGGCCGTCCCACCAGCTGATTTTCCAGGCGATCATTGACCTGTTCAGTGACAACCGTGATATTGACCCCG
TGATTGTCTCCGGTCGCCTCGATCGAACCAATGATCTGGACCGCGTCGGCGGTGGCGCGTACCTCCACGACCTCATCCAG
TCCGTTCCCACCGCAGCCAACGCGCGCTACTACGCGGAAATCGTCTCGGAAAAGGCAGTGCTTCGCAGGCTTGTCGACGC
TGGCACCCGCGTCGTCCAGCTCGGCTACGAGGGCGATGAGGGCGCCGAAATCGACGCGGTGATTGACCGCGCGCAGCAAG
AAGTCTTCGCCGTTTCCCAAAAGAATCAGAGCGAAGACTATGCAGTCCTAGCCGATATTCTGGATGAAACCATGGCTGAG
CTGGAAATGCTCAACGACGGCGGCATCGCCACCGGTATTCCAACCGGCTTTAAGGATCTCGATGACCTCACCAACGGTCT
GCGCGGTGGCCAGATGATTATCGTTGCAGCTCGTCCTGGTGTGGGTAAATCCACCATTGCTTTGGACTTCATGCGTTCCG
CATCCATCAAGAACAACATGGCGTCTGTCATTTTCTCCTTGGAAATGTCCAAGTCAGAGATCGTGATGCGTTTGCTCTCT
GCTGAAACAGAAATCCGCCTGGCTGATATGCGTGGTGGTAAGATGGATGAAACCGCATGGGAAAAGATGGTGCAGAAGTT
GGACAAGGTAGCCCAGGCACCGTTGTTCATCGATGACTCTGCGAACCTCACCATGATGGAAATCCGCTCCAAGGCCAGAA
AGCTGAAGCAAAAGCATGATCTGAAAATGATCGTGGTGGACTACCTCCAGCTGATGAGCTCCGGTAAACGCGTGGAATCC
CGTCAGCAGGAAGTCTCCGAGTTCTCCCGTCAGCTCAAGCTGCTGGCCAAAGAACTCGATGTGCCGTTGATCGCGATTTC
CCAGCTGAACCGTGGACCTGAATCCCGTACCGATAAGCGACCACAGCTTGCTGACCTTCGTGAATCCGGCTCGCTGGAGC
AGGACGCCGATATCGTTATGCTGCTATACCGCCCAGACTCCCAGGATAAGGACGACGAGCGCGCAGGCGAGGCCGACATC
ATTTTGGCTAAGCACCGTGGTGGCCCGATCGATACCGTCCAGGTGGCGCACCAGCTGCACTATTCACGTTTTGTGGACAT
GGCGCGCGGTTAA

Upstream 100 bases:

>100_bases
GAGTTGGAAATCACAAAGCCTCGGGGTGGGGTTTTCGTGCCTTCTGGAAAGACCTATGTAGGGGCTAGAGTGAACTTAGA
ATCAGATTGGGGAAAATAAA

Downstream 100 bases:

>100_bases
AACAGTTCATTTTTCTAAAGGCAGTGACTCCGATGTGGGTCGCTGCCTTTAGTCATTTTAAGTCACTCAGCCCAGGCAAT
CCTATCTTTGGAAAGACTTA

Product: replicative DNA helicase

Products: NA

Alternate protein names: Mbo dnaB intein [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MATDTHAASFDDDYVPAQEPSDSFADDAHVDVPAPAFEDFSPAQAFGQGTRGGDSQGFKKRGRKDESREYRDFRQPPYDN
DAEMGVLGAMLLSPTTVIDILDILTPEDFYRPSHQLIFQAIIDLFSDNRDIDPVIVSGRLDRTNDLDRVGGGAYLHDLIQ
SVPTAANARYYAEIVSEKAVLRRLVDAGTRVVQLGYEGDEGAEIDAVIDRAQQEVFAVSQKNQSEDYAVLADILDETMAE
LEMLNDGGIATGIPTGFKDLDDLTNGLRGGQMIIVAARPGVGKSTIALDFMRSASIKNNMASVIFSLEMSKSEIVMRLLS
AETEIRLADMRGGKMDETAWEKMVQKLDKVAQAPLFIDDSANLTMMEIRSKARKLKQKHDLKMIVVDYLQLMSSGKRVES
RQQEVSEFSRQLKLLAKELDVPLIAISQLNRGPESRTDKRPQLADLRESGSLEQDADIVMLLYRPDSQDKDDERAGEADI
ILAKHRGGPIDTVQVAHQLHYSRFVDMARG

Sequences:

>Translated_510_residues
MATDTHAASFDDDYVPAQEPSDSFADDAHVDVPAPAFEDFSPAQAFGQGTRGGDSQGFKKRGRKDESREYRDFRQPPYDN
DAEMGVLGAMLLSPTTVIDILDILTPEDFYRPSHQLIFQAIIDLFSDNRDIDPVIVSGRLDRTNDLDRVGGGAYLHDLIQ
SVPTAANARYYAEIVSEKAVLRRLVDAGTRVVQLGYEGDEGAEIDAVIDRAQQEVFAVSQKNQSEDYAVLADILDETMAE
LEMLNDGGIATGIPTGFKDLDDLTNGLRGGQMIIVAARPGVGKSTIALDFMRSASIKNNMASVIFSLEMSKSEIVMRLLS
AETEIRLADMRGGKMDETAWEKMVQKLDKVAQAPLFIDDSANLTMMEIRSKARKLKQKHDLKMIVVDYLQLMSSGKRVES
RQQEVSEFSRQLKLLAKELDVPLIAISQLNRGPESRTDKRPQLADLRESGSLEQDADIVMLLYRPDSQDKDDERAGEADI
ILAKHRGGPIDTVQVAHQLHYSRFVDMARG
>Mature_509_residues
ATDTHAASFDDDYVPAQEPSDSFADDAHVDVPAPAFEDFSPAQAFGQGTRGGDSQGFKKRGRKDESREYRDFRQPPYDND
AEMGVLGAMLLSPTTVIDILDILTPEDFYRPSHQLIFQAIIDLFSDNRDIDPVIVSGRLDRTNDLDRVGGGAYLHDLIQS
VPTAANARYYAEIVSEKAVLRRLVDAGTRVVQLGYEGDEGAEIDAVIDRAQQEVFAVSQKNQSEDYAVLADILDETMAEL
EMLNDGGIATGIPTGFKDLDDLTNGLRGGQMIIVAARPGVGKSTIALDFMRSASIKNNMASVIFSLEMSKSEIVMRLLSA
ETEIRLADMRGGKMDETAWEKMVQKLDKVAQAPLFIDDSANLTMMEIRSKARKLKQKHDLKMIVVDYLQLMSSGKRVESR
QQEVSEFSRQLKLLAKELDVPLIAISQLNRGPESRTDKRPQLADLRESGSLEQDADIVMLLYRPDSQDKDDERAGEADII
LAKHRGGPIDTVQVAHQLHYSRFVDMARG

Specific function: The intein is an endonuclease (Potential) [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=444, Percent_Identity=38.2882882882883, Blast_Score=305, Evalue=4e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 56563; Mature: 56432

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDTHAASFDDDYVPAQEPSDSFADDAHVDVPAPAFEDFSPAQAFGQGTRGGDSQGFKK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHH
RGRKDESREYRDFRQPPYDNDAEMGVLGAMLLSPTTVIDILDILTPEDFYRPSHQLIFQA
CCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCHHHCCCHHHHHHHH
IIDLFSDNRDIDPVIVSGRLDRTNDLDRVGGGAYLHDLIQSVPTAANARYYAEIVSEKAV
HHHHHCCCCCCCCEEEECCCCCCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LRRLVDAGTRVVQLGYEGDEGAEIDAVIDRAQQEVFAVSQKNQSEDYAVLADILDETMAE
HHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LEMLNDGGIATGIPTGFKDLDDLTNGLRGGQMIIVAARPGVGKSTIALDFMRSASIKNNM
HHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
ASVIFSLEMSKSEIVMRLLSAETEIRLADMRGGKMDETAWEKMVQKLDKVAQAPLFIDDS
HHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECC
ANLTMMEIRSKARKLKQKHDLKMIVVDYLQLMSSGKRVESRQQEVSEFSRQLKLLAKELD
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
VPLIAISQLNRGPESRTDKRPQLADLRESGSLEQDADIVMLLYRPDSQDKDDERAGEADI
CCEEEHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHCCCCEE
ILAKHRGGPIDTVQVAHQLHYSRFVDMARG
EEEECCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATDTHAASFDDDYVPAQEPSDSFADDAHVDVPAPAFEDFSPAQAFGQGTRGGDSQGFKK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHH
RGRKDESREYRDFRQPPYDNDAEMGVLGAMLLSPTTVIDILDILTPEDFYRPSHQLIFQA
CCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCHHHCCCHHHHHHHH
IIDLFSDNRDIDPVIVSGRLDRTNDLDRVGGGAYLHDLIQSVPTAANARYYAEIVSEKAV
HHHHHCCCCCCCCEEEECCCCCCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LRRLVDAGTRVVQLGYEGDEGAEIDAVIDRAQQEVFAVSQKNQSEDYAVLADILDETMAE
HHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LEMLNDGGIATGIPTGFKDLDDLTNGLRGGQMIIVAARPGVGKSTIALDFMRSASIKNNM
HHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
ASVIFSLEMSKSEIVMRLLSAETEIRLADMRGGKMDETAWEKMVQKLDKVAQAPLFIDDS
HHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECC
ANLTMMEIRSKARKLKQKHDLKMIVVDYLQLMSSGKRVESRQQEVSEFSRQLKLLAKELD
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
VPLIAISQLNRGPESRTDKRPQLADLRESGSLEQDADIVMLLYRPDSQDKDDERAGEADI
CCEEEHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHCCCCEE
ILAKHRGGPIDTVQVAHQLHYSRFVDMARG
EEEECCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]