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The map label for this gene is gatA [H]

Identifier: 145296956

GI number: 145296956

Start: 3166571

End: 3167983

Strand: Direct

Name: gatA [H]

Synonym: cgR_2856

Alternate gene names: 145296956

Gene position: 3166571-3167983 (Clockwise)

Preceding gene: 145296955

Following gene: 145296958

Centisome position: 95.55

GC content: 51.38

Gene sequence:

>1413_bases
TTGCCTAAGGAACTACTGAAAAAGACAGCTACTGAGGCTGGAGAACTTATTCATAAAGGCGAAGTTACCTCTGTCGAACT
AACTCAATCTCTTCTCGAGCATGCCCATTCGCTCAATGAACAAGTGAATGCTTATGTTTCATTCCGCGATGATAAAGCAC
TTGAAGAAGCCCGAAAAGCCGATGAAGAAATCGCCGCCGGAAACATCCGTGGCCCATTGCATGGTGTGCCGATGGGTATT
AAAGACAACCTTTATATGGCAGACGAGGTCACCACAATGGCCTCCAAGATCCACGGCGATTTTGTATCAAAGCATGATGC
CAGCGCAGTAACCGGCCTGAAAAATGCTGGAGTTGTCATCACCGGAAAACTAAACATGCATGAGTACGCATGGGGAATCG
ATAACAACAGCCCTCACTTTGGACCAGTCCACAACCCGTGGAATCCAGACAAAGTCCCTGGTGGATCATCTGGAGGCTCT
GGTGCCGCAGTAGCTTCTGACATGTCCTTCTCGTCCCTGGGCACCGACACCGCTGGCTCAATTCGCATTCCATCCTCTGC
GTGTGGTCTCGTTGGCCTTAAGCCAACCCATGGCCGAGTCGCAAAATTTGGTTGTTTCCCCTTAGCCTGGTCCCTAGATC
ATATCGGCCCAATGTCTAAAAATGTGGCGGATGCTGCAGCGATGCTTCAAGCTATCGCAGGATTCGATCACCGAGATCCG
ACTTCAGTCAACGTCCCAGTAGGCAACTACAGTGCAGCTCTGGGGGAAAGCGCTGAAGGACTAGTTATCGGAGTCGAAGA
AGATTACTTCTTCAAGGATGTCGATAATGAAGTCGAGCGCCTTGTTCGTGCTCAAATCGATGAACTTGTCAGCCGTGGCG
CAAAGGTCAAGAAGGTAGAGATCCCAGCTCTGGCATATTCAGAGTGGACAGAGTTGGCAACAAGTCTCTCGGAAGCTTCC
GCGATTCACCACAATGATCTGATCAACCGTGCCGACGATTTTGGTGACGATATCCGATTCCTCTTCGAACTCGGTGAACT
GTTCTCCTCCGTCGACTACTTGCAGGCACAACAAGCACGCAGGCAGATCAAGCAAGAATTTGCCCAAGCACTTTCCGACG
TTGATGTCATCATCGCACCAACGCTGCCTGTGATGGCACCGGACATTGGATCTGCAGTGGCAGACCTCAACGGTAAGGAC
GTCGACCTCATCGACAGCTTCATCCGATTTACCGGTCCAAGCAACCTGACTGGACTTCCTGCACTGACTGTTCCAGCAGG
CCTCAACGGTGGTCTCCCCGTAGGACTGCAGGTCATCGGGCGCGCATTCGATGAAGAAACAGTTCTTAAGGTTGGATCAA
TCATCGAATCCGGTGATCCAATGGGGGGCGCTCGAGCACCAATTGGTGCCTAG

Upstream 100 bases:

>100_bases
GTAAGTGGGAAGAGATCGAGGGACTTAAGGGCAACCTCGCAAACATCAATCTTGATGATGCAGATATCTCTCCTCCGCAA
CATCCCAGGAGGCGATCACG

Downstream 100 bases:

>100_bases
CCTCCACCTCTAACCCAATTACGCAACAATCTAATGCCCGGTCTGTTTGACAAGCAGACCGGGCATTGGACTATCCGCTT
ACTTAAGTTACGAATCCGAT

Product: hypothetical protein

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MPKELLKKTATEAGELIHKGEVTSVELTQSLLEHAHSLNEQVNAYVSFRDDKALEEARKADEEIAAGNIRGPLHGVPMGI
KDNLYMADEVTTMASKIHGDFVSKHDASAVTGLKNAGVVITGKLNMHEYAWGIDNNSPHFGPVHNPWNPDKVPGGSSGGS
GAAVASDMSFSSLGTDTAGSIRIPSSACGLVGLKPTHGRVAKFGCFPLAWSLDHIGPMSKNVADAAAMLQAIAGFDHRDP
TSVNVPVGNYSAALGESAEGLVIGVEEDYFFKDVDNEVERLVRAQIDELVSRGAKVKKVEIPALAYSEWTELATSLSEAS
AIHHNDLINRADDFGDDIRFLFELGELFSSVDYLQAQQARRQIKQEFAQALSDVDVIIAPTLPVMAPDIGSAVADLNGKD
VDLIDSFIRFTGPSNLTGLPALTVPAGLNGGLPVGLQVIGRAFDEETVLKVGSIIESGDPMGGARAPIGA

Sequences:

>Translated_470_residues
MPKELLKKTATEAGELIHKGEVTSVELTQSLLEHAHSLNEQVNAYVSFRDDKALEEARKADEEIAAGNIRGPLHGVPMGI
KDNLYMADEVTTMASKIHGDFVSKHDASAVTGLKNAGVVITGKLNMHEYAWGIDNNSPHFGPVHNPWNPDKVPGGSSGGS
GAAVASDMSFSSLGTDTAGSIRIPSSACGLVGLKPTHGRVAKFGCFPLAWSLDHIGPMSKNVADAAAMLQAIAGFDHRDP
TSVNVPVGNYSAALGESAEGLVIGVEEDYFFKDVDNEVERLVRAQIDELVSRGAKVKKVEIPALAYSEWTELATSLSEAS
AIHHNDLINRADDFGDDIRFLFELGELFSSVDYLQAQQARRQIKQEFAQALSDVDVIIAPTLPVMAPDIGSAVADLNGKD
VDLIDSFIRFTGPSNLTGLPALTVPAGLNGGLPVGLQVIGRAFDEETVLKVGSIIESGDPMGGARAPIGA
>Mature_469_residues
PKELLKKTATEAGELIHKGEVTSVELTQSLLEHAHSLNEQVNAYVSFRDDKALEEARKADEEIAAGNIRGPLHGVPMGIK
DNLYMADEVTTMASKIHGDFVSKHDASAVTGLKNAGVVITGKLNMHEYAWGIDNNSPHFGPVHNPWNPDKVPGGSSGGSG
AAVASDMSFSSLGTDTAGSIRIPSSACGLVGLKPTHGRVAKFGCFPLAWSLDHIGPMSKNVADAAAMLQAIAGFDHRDPT
SVNVPVGNYSAALGESAEGLVIGVEEDYFFKDVDNEVERLVRAQIDELVSRGAKVKKVEIPALAYSEWTELATSLSEASA
IHHNDLINRADDFGDDIRFLFELGELFSSVDYLQAQQARRQIKQEFAQALSDVDVIIAPTLPVMAPDIGSAVADLNGKDV
DLIDSFIRFTGPSNLTGLPALTVPAGLNGGLPVGLQVIGRAFDEETVLKVGSIIESGDPMGGARAPIGA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=502, Percent_Identity=30.8764940239044, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI195972892, Length=504, Percent_Identity=25, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI166795287, Length=257, Percent_Identity=30.3501945525292, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=457, Percent_Identity=27.5711159737418, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17537465, Length=243, Percent_Identity=31.2757201646091, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=297, Percent_Identity=26.5993265993266, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=258, Percent_Identity=28.2945736434109, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=314, Percent_Identity=28.343949044586, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=198, Percent_Identity=27.7777777777778, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=198, Percent_Identity=27.7777777777778, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=280, Percent_Identity=27.8571428571429, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=461, Percent_Identity=28.1995661605206, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319685, Length=447, Percent_Identity=28.6353467561521, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320448, Length=507, Percent_Identity=24.65483234714, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648113, Length=477, Percent_Identity=30.8176100628931, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI161078093, Length=506, Percent_Identity=28.0632411067194, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24652985, Length=507, Percent_Identity=26.4299802761341, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI19922090, Length=507, Percent_Identity=26.4299802761341, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24652981, Length=507, Percent_Identity=26.4299802761341, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24652983, Length=507, Percent_Identity=26.4299802761341, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=24.8496993987976, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI21356731, Length=482, Percent_Identity=24.6887966804979, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24648435, Length=501, Percent_Identity=25.1497005988024, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648437, Length=501, Percent_Identity=25.1497005988024, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45550774, Length=501, Percent_Identity=25.1497005988024, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648441, Length=429, Percent_Identity=24.009324009324, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648439, Length=429, Percent_Identity=24.009324009324, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49768; Mature: 49637

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKELLKKTATEAGELIHKGEVTSVELTQSLLEHAHSLNEQVNAYVSFRDDKALEEARKA
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHH
DEEIAAGNIRGPLHGVPMGIKDNLYMADEVTTMASKIHGDFVSKHDASAVTGLKNAGVVI
HHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEE
TGKLNMHEYAWGIDNNSPHFGPVHNPWNPDKVPGGSSGGSGAAVASDMSFSSLGTDTAGS
EEECCHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHCCCCCCCC
IRIPSSACGLVGLKPTHGRVAKFGCFPLAWSLDHIGPMSKNVADAAAMLQAIAGFDHRDP
EECCCCCCCCEECCCCCCCEEEECCCHHEECHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
TSVNVPVGNYSAALGESAEGLVIGVEEDYFFKDVDNEVERLVRAQIDELVSRGAKVKKVE
CEEECCCCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IPALAYSEWTELATSLSEASAIHHNDLINRADDFGDDIRFLFELGELFSSVDYLQAQQAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RQIKQEFAQALSDVDVIIAPTLPVMAPDIGSAVADLNGKDVDLIDSFIRFTGPSNLTGLP
HHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC
ALTVPAGLNGGLPVGLQVIGRAFDEETVLKVGSIIESGDPMGGARAPIGA
CEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PKELLKKTATEAGELIHKGEVTSVELTQSLLEHAHSLNEQVNAYVSFRDDKALEEARKA
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHH
DEEIAAGNIRGPLHGVPMGIKDNLYMADEVTTMASKIHGDFVSKHDASAVTGLKNAGVVI
HHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEE
TGKLNMHEYAWGIDNNSPHFGPVHNPWNPDKVPGGSSGGSGAAVASDMSFSSLGTDTAGS
EEECCHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHCCCCCCCC
IRIPSSACGLVGLKPTHGRVAKFGCFPLAWSLDHIGPMSKNVADAAAMLQAIAGFDHRDP
EECCCCCCCCEECCCCCCCEEEECCCHHEECHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
TSVNVPVGNYSAALGESAEGLVIGVEEDYFFKDVDNEVERLVRAQIDELVSRGAKVKKVE
CEEECCCCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IPALAYSEWTELATSLSEASAIHHNDLINRADDFGDDIRFLFELGELFSSVDYLQAQQAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RQIKQEFAQALSDVDVIIAPTLPVMAPDIGSAVADLNGKDVDLIDSFIRFTGPSNLTGLP
HHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCC
ALTVPAGLNGGLPVGLQVIGRAFDEETVLKVGSIIESGDPMGGARAPIGA
CEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA