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The map label for this gene is shiA [H]

Identifier: 145296918

GI number: 145296918

Start: 3130172

End: 3131473

Strand: Direct

Name: shiA [H]

Synonym: cgR_2818

Alternate gene names: 145296918

Gene position: 3130172-3131473 (Clockwise)

Preceding gene: 145296917

Following gene: 145296919

Centisome position: 94.45

GC content: 55.84

Gene sequence:

>1302_bases
ATGACAACCGCAGTAGATCAAAACTCACCGCCCAAGCAGCAACTCAACAAGCGCGTCCTGCTGGGCAGCTTGAGTGGCAG
CGTTATCGAATGGTTCGACTTCCTGGTTTACGGAACCGTCGCCGCGCTGGTCTTCAACAAGATGTACTTCCCCAGTGACA
ACGAGTTCCTCTCCACAATCCTGGCGTACGCTTCCTTCTCCCTGACCTTCTTCTTCCGTCCCGTTGGCGGCGTCATCTTC
GCCCACATCGGTGACCGCATTGGTCGTAAGAAGACCCTGTTCATCACTTTGATGCTCATGGGTGGCGGTACCGTCGCCAT
TGGTTTGCTCCCCGACTACAACTCTATCGGCATTTGGGCACCAATCCTCCTGATGTTCCTCCGCATCCTGCAGGGCATCG
GCATCGGTGGCGAATGGGGTGGCGCACTGCTGCTCGCGTACGAATACGCACCAAAGAAGCAGCGCGGACTCTACGGCGCA
GTTCCACAAATGGGCATCTCCCTGGGCATGCTCCTTGCTGCCGGCGTAATCTCCCTGCTCACCCTCATGCCGGAAGATCA
GTTCCTCACCTGGGGCTGGCGTATCCCATTCGTTGGATCTATCCTCCTGGTGTTCATCGGCTTGTTCATCCGAAACGGCC
TTGATGAGACCCCAGAATTCAAGCGCATCCGCGATTCCGGCCAGCAGGTAAAGATGCCACTGAAGGAAGTCCTGACCAAG
TACTGGCCAGCCGTGTTGGTTTCCATCGGAGCAAAAGCTGCAGAGACCGGCCCGTTCTACATCTTCGGTACCTACATCGT
TGCTTACGCAACCAACTTCCTGAACATCCGCGATAACATTGTTCTTCTGGCAGTTGCTTGTGCAGCCCTTGTTGCCACCA
TCTGGATGCCACTGTTCGGATCCTTCTCCGACCGTGTCAACCGAGCAGCCCTCTACAGGATCTGTGCATCTGCAACCATC
GTGTTTATCGTCCCTTACTACTTGATCCTTAACACCGGCGAAATTTGGGCACTATTTATCACCACCGTGATTGGCTTCGG
CATCCTCTGGGGTAGCGTCAACGCAATCCTCGGAACCGTCATCGCAGAAAACTTCGCACCTGAGGTCCGCTACACCGGCG
CTACCCTGGGTTACCAAGTCGGAGCAGCACTCTTCGGCGGTACCGCACCCATCATCGCAGCATGGCTGTTCGAAATCTCC
GGCGGACAATGGTGGCCAATCGCCGTCTACGTCGTCGCATGTTGCCTCCTATCTGTGATCGCCTCGTTCTTCATCCAACG
CGTCGCACACCAAGAGAACTAA

Upstream 100 bases:

>100_bases
AAAGGTGACATGCCTTACATTCTTGTGGTCTGACCATGAGGTTGGGCCAATCGGTTTCAGCCCGTTTACTCCCGCCGTCC
GTTTCAGAGAAGAGGTCACC

Downstream 100 bases:

>100_bases
AATCCAAGTAAAACCCCTCCGAAAGGAACCACCCATGGTGAAACGTCAACTGCCCAACCCCGCAGAACTACTCGAACTCA
TGAAGTTCAAAAAGCCAGAG

Product: hypothetical protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTTAVDQNSPPKQQLNKRVLLGSLSGSVIEWFDFLVYGTVAALVFNKMYFPSDNEFLSTILAYASFSLTFFFRPVGGVIF
AHIGDRIGRKKTLFITLMLMGGGTVAIGLLPDYNSIGIWAPILLMFLRILQGIGIGGEWGGALLLAYEYAPKKQRGLYGA
VPQMGISLGMLLAAGVISLLTLMPEDQFLTWGWRIPFVGSILLVFIGLFIRNGLDETPEFKRIRDSGQQVKMPLKEVLTK
YWPAVLVSIGAKAAETGPFYIFGTYIVAYATNFLNIRDNIVLLAVACAALVATIWMPLFGSFSDRVNRAALYRICASATI
VFIVPYYLILNTGEIWALFITTVIGFGILWGSVNAILGTVIAENFAPEVRYTGATLGYQVGAALFGGTAPIIAAWLFEIS
GGQWWPIAVYVVACCLLSVIASFFIQRVAHQEN

Sequences:

>Translated_433_residues
MTTAVDQNSPPKQQLNKRVLLGSLSGSVIEWFDFLVYGTVAALVFNKMYFPSDNEFLSTILAYASFSLTFFFRPVGGVIF
AHIGDRIGRKKTLFITLMLMGGGTVAIGLLPDYNSIGIWAPILLMFLRILQGIGIGGEWGGALLLAYEYAPKKQRGLYGA
VPQMGISLGMLLAAGVISLLTLMPEDQFLTWGWRIPFVGSILLVFIGLFIRNGLDETPEFKRIRDSGQQVKMPLKEVLTK
YWPAVLVSIGAKAAETGPFYIFGTYIVAYATNFLNIRDNIVLLAVACAALVATIWMPLFGSFSDRVNRAALYRICASATI
VFIVPYYLILNTGEIWALFITTVIGFGILWGSVNAILGTVIAENFAPEVRYTGATLGYQVGAALFGGTAPIIAAWLFEIS
GGQWWPIAVYVVACCLLSVIASFFIQRVAHQEN
>Mature_432_residues
TTAVDQNSPPKQQLNKRVLLGSLSGSVIEWFDFLVYGTVAALVFNKMYFPSDNEFLSTILAYASFSLTFFFRPVGGVIFA
HIGDRIGRKKTLFITLMLMGGGTVAIGLLPDYNSIGIWAPILLMFLRILQGIGIGGEWGGALLLAYEYAPKKQRGLYGAV
PQMGISLGMLLAAGVISLLTLMPEDQFLTWGWRIPFVGSILLVFIGLFIRNGLDETPEFKRIRDSGQQVKMPLKEVLTKY
WPAVLVSIGAKAAETGPFYIFGTYIVAYATNFLNIRDNIVLLAVACAALVATIWMPLFGSFSDRVNRAALYRICASATIV
FIVPYYLILNTGEIWALFITTVIGFGILWGSVNAILGTVIAENFAPEVRYTGATLGYQVGAALFGGTAPIIAAWLFEISG
GQWWPIAVYVVACCLLSVIASFFIQRVAHQEN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=434, Percent_Identity=36.6359447004608, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=32.9439252336449, Blast_Score=241, Evalue=7e-65,
Organism=Escherichia coli, GI1789941, Length=436, Percent_Identity=37.8440366972477, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1788942, Length=401, Percent_Identity=32.6683291770574, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI87082231, Length=292, Percent_Identity=27.0547945205479, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082404, Length=406, Percent_Identity=23.6453201970443, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47375; Mature: 47243

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAVDQNSPPKQQLNKRVLLGSLSGSVIEWFDFLVYGTVAALVFNKMYFPSDNEFLSTI
CCCCCCCCCCCHHHHCHHEEHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LAYASFSLTFFFRPVGGVIFAHIGDRIGRKKTLFITLMLMGGGTVAIGLLPDYNSIGIWA
HHHHHHHHHEEEEHHHHHHHHHHHHHHCCCEEEEEEEEEECCCEEEEEECCCCCCCCHHH
PILLMFLRILQGIGIGGEWGGALLLAYEYAPKKQRGLYGAVPQMGISLGMLLAAGVISLL
HHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHH
TLMPEDQFLTWGWRIPFVGSILLVFIGLFIRNGLDETPEFKRIRDSGQQVKMPLKEVLTK
HHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHCCHHHHHHH
YWPAVLVSIGAKAAETGPFYIFGTYIVAYATNFLNIRDNIVLLAVACAALVATIWMPLFG
HHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
SFSDRVNRAALYRICASATIVFIVPYYLILNTGEIWALFITTVIGFGILWGSVNAILGTV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAENFAPEVRYTGATLGYQVGAALFGGTAPIIAAWLFEISGGQWWPIAVYVVACCLLSVI
HHHHCCCCCEECCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ASFFIQRVAHQEN
HHHHHHHHHHCCC
>Mature Secondary Structure 
TTAVDQNSPPKQQLNKRVLLGSLSGSVIEWFDFLVYGTVAALVFNKMYFPSDNEFLSTI
CCCCCCCCCCHHHHCHHEEHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LAYASFSLTFFFRPVGGVIFAHIGDRIGRKKTLFITLMLMGGGTVAIGLLPDYNSIGIWA
HHHHHHHHHEEEEHHHHHHHHHHHHHHCCCEEEEEEEEEECCCEEEEEECCCCCCCCHHH
PILLMFLRILQGIGIGGEWGGALLLAYEYAPKKQRGLYGAVPQMGISLGMLLAAGVISLL
HHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHH
TLMPEDQFLTWGWRIPFVGSILLVFIGLFIRNGLDETPEFKRIRDSGQQVKMPLKEVLTK
HHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHCCHHHHHHH
YWPAVLVSIGAKAAETGPFYIFGTYIVAYATNFLNIRDNIVLLAVACAALVATIWMPLFG
HHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
SFSDRVNRAALYRICASATIVFIVPYYLILNTGEIWALFITTVIGFGILWGSVNAILGTV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAENFAPEVRYTGATLGYQVGAALFGGTAPIIAAWLFEISGGQWWPIAVYVVACCLLSVI
HHHHCCCCCEECCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ASFFIQRVAHQEN
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]