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The map label for this gene is aspA [H]

Identifier: 145296909

GI number: 145296909

Start: 3118892

End: 3120301

Strand: Direct

Name: aspA [H]

Synonym: cgR_2809

Alternate gene names: 145296909

Gene position: 3118892-3120301 (Clockwise)

Preceding gene: 145296908

Following gene: 145296910

Centisome position: 94.11

GC content: 58.51

Gene sequence:

>1410_bases
ATGACCACCACTTTTAGCTCCACCCGCACCGAACATGACCTCATCGGCGACGCCGAAGTCCCAGCTCACGTGTATTGGGG
CGTTCACACCATGCGCGCTGCAGAGAATTTCCAGATCACCGATCAAAAGCTTGCCGATAATCCTTTCCTCATCGCAGCTC
TTGCGCGCGTCAAACAGGCCGCCGCCCGCGCCAACCTACAACTTGGACTGCTTGATCAAACTCGCGCTGAAGCAATCGAT
CAGGCCTGTGACCGCGTTGCTGCCGGAGAATTCAATGAACAATTCATTCTCGACCCGATTCAGGGCGGCGCTGGCACAAG
CACCAACATGAATGCCAATGAGGTCATTGCCAACGTAGCGCTGGAGATTCTTGGGCATGAAAAGGGCGACTACCAGCACC
TTCACCCCAATGATCACGTGAATCTTTCACAATCCACCAACGATGCATACCCCACGGCAGTAAAAATCGCCACCTGTTTT
GCCGCCCGCCCGCTGCTCGACGCCCTGACTGAGCTTGAGCAAGCATGCCGCAGCAAAGCCGTGGAATTTCGGACCGTAGT
GAAAATGGGACGCACCCAACTGCAAGACGCCGTCCCCATGACCGTCGGCCAAGAATTCGGCGGATGGGCCGTCACACTGC
GGGAAGACCACGATCGTTTAGAGGAATCCCTCAAGCTCGTCACCGAAATCAACCTCGGCGCAACCGCGATCGGCACCGGA
CTTAACGCACCAGAAGGATACGCCGAAGCCGCACGCAAGCACCTGGCAGAACTCACCGCCCTGCCGCTGTCCACCAGCCC
ACACCTCGTCGAAGCAACCAGCGACGTCGGAGCCTTCGTCCACCTCTCCGGAGTACTCAAACGCGTCGCCGTGAAACTCT
CCAAAATCTGCAGCGACCTGCGACTACTCTCCTCCGGGCCACGCGCAGGCCTCAACGACATCCAACTACCCGCTGTACAA
TCCGGCTCCTCCATCATGCCCGGCAAAGTCAACCCCGTCATCCCTGAAGTTGTCAACCAAATCGCCTACCAAGTAGTCGG
CTACGACGCCACCGTCACCATGGCCGCAGAAGGCGGACAACTCCAGCTCAACGCCTTCGAACCCGTCATCATGCACTCTA
TCGGACTGTCGATGAAACACCTCGCCAACGGCTGTAGCACGCTAGCCCGCAAGTGCATTGCGGGCATCACGGTCGACGAA
GATGCTTTACGACGCCAAGTTGAACAATCAATCGGCCTCGTCACCGCACTCAACCCCGCCCTTGGCTATACCCAATCCAC
CATGATCGCCATGGAAGCCCTCCACACCGGACGCGGCGTTGCCGAACTCGTCCTCGAACGTGAGCTCCTCACCGAAGCCG
AACTCTCCGAACTGCTTCGCCCCGAACGACTCGCCAACCTCGCCTCCTAG

Upstream 100 bases:

>100_bases
TGCCGGATGACCTGCGCGCCCTAACCCACAGCAATGTTGAAGCAGGGCCGGCGGAGGAACGCGTCGGCAAGCTATTCGCC
CTTTAAAAGAAAGCACTGTC

Downstream 100 bases:

>100_bases
AAACGAGCACACCATGTCAACTACTCAAGCTAAGGGTACGACTGCATCTTCTGCTTCTCCTGCTTCTCCTGCTGATGCCG
CCTTTGATTCCGCTTTAACC

Product: hypothetical protein

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTTTFSSTRTEHDLIGDAEVPAHVYWGVHTMRAAENFQITDQKLADNPFLIAALARVKQAAARANLQLGLLDQTRAEAID
QACDRVAAGEFNEQFILDPIQGGAGTSTNMNANEVIANVALEILGHEKGDYQHLHPNDHVNLSQSTNDAYPTAVKIATCF
AARPLLDALTELEQACRSKAVEFRTVVKMGRTQLQDAVPMTVGQEFGGWAVTLREDHDRLEESLKLVTEINLGATAIGTG
LNAPEGYAEAARKHLAELTALPLSTSPHLVEATSDVGAFVHLSGVLKRVAVKLSKICSDLRLLSSGPRAGLNDIQLPAVQ
SGSSIMPGKVNPVIPEVVNQIAYQVVGYDATVTMAAEGGQLQLNAFEPVIMHSIGLSMKHLANGCSTLARKCIAGITVDE
DALRRQVEQSIGLVTALNPALGYTQSTMIAMEALHTGRGVAELVLERELLTEAELSELLRPERLANLAS

Sequences:

>Translated_469_residues
MTTTFSSTRTEHDLIGDAEVPAHVYWGVHTMRAAENFQITDQKLADNPFLIAALARVKQAAARANLQLGLLDQTRAEAID
QACDRVAAGEFNEQFILDPIQGGAGTSTNMNANEVIANVALEILGHEKGDYQHLHPNDHVNLSQSTNDAYPTAVKIATCF
AARPLLDALTELEQACRSKAVEFRTVVKMGRTQLQDAVPMTVGQEFGGWAVTLREDHDRLEESLKLVTEINLGATAIGTG
LNAPEGYAEAARKHLAELTALPLSTSPHLVEATSDVGAFVHLSGVLKRVAVKLSKICSDLRLLSSGPRAGLNDIQLPAVQ
SGSSIMPGKVNPVIPEVVNQIAYQVVGYDATVTMAAEGGQLQLNAFEPVIMHSIGLSMKHLANGCSTLARKCIAGITVDE
DALRRQVEQSIGLVTALNPALGYTQSTMIAMEALHTGRGVAELVLERELLTEAELSELLRPERLANLAS
>Mature_468_residues
TTTFSSTRTEHDLIGDAEVPAHVYWGVHTMRAAENFQITDQKLADNPFLIAALARVKQAAARANLQLGLLDQTRAEAIDQ
ACDRVAAGEFNEQFILDPIQGGAGTSTNMNANEVIANVALEILGHEKGDYQHLHPNDHVNLSQSTNDAYPTAVKIATCFA
ARPLLDALTELEQACRSKAVEFRTVVKMGRTQLQDAVPMTVGQEFGGWAVTLREDHDRLEESLKLVTEINLGATAIGTGL
NAPEGYAEAARKHLAELTALPLSTSPHLVEATSDVGAFVHLSGVLKRVAVKLSKICSDLRLLSSGPRAGLNDIQLPAVQS
GSSIMPGKVNPVIPEVVNQIAYQVVGYDATVTMAAEGGQLQLNAFEPVIMHSIGLSMKHLANGCSTLARKCIAGITVDED
ALRRQVEQSIGLVTALNPALGYTQSTMIAMEALHTGRGVAELVLERELLTEAELSELLRPERLANLAS

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=460, Percent_Identity=40, Blast_Score=331, Evalue=9e-91,
Organism=Escherichia coli, GI87082375, Length=460, Percent_Identity=53.695652173913, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1787896, Length=461, Percent_Identity=41.4316702819957, Blast_Score=352, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI17553882, Length=464, Percent_Identity=40.948275862069, Blast_Score=354, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI32565146, Length=324, Percent_Identity=39.1975308641975, Blast_Score=244, Evalue=9e-65,
Organism=Saccharomyces cerevisiae, GI6324993, Length=460, Percent_Identity=40.8695652173913, Blast_Score=343, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24640177, Length=458, Percent_Identity=41.0480349344978, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24640179, Length=458, Percent_Identity=41.0480349344978, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI78710009, Length=459, Percent_Identity=40.7407407407407, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24662684, Length=466, Percent_Identity=40.5579399141631, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24583245, Length=464, Percent_Identity=36.2068965517241, Blast_Score=289, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 50285; Mature: 50154

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTFSSTRTEHDLIGDAEVPAHVYWGVHTMRAAENFQITDQKLADNPFLIAALARVKQA
CCCCCCCCCCHHHCCCCCCCCEEEEECHHHHHHCCCCEECHHHHCCCCHHHHHHHHHHHH
AARANLQLGLLDQTRAEAIDQACDRVAAGEFNEQFILDPIQGGAGTSTNMNANEVIANVA
HHHCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHH
LEILGHEKGDYQHLHPNDHVNLSQSTNDAYPTAVKIATCFAARPLLDALTELEQACRSKA
HHHHCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEFRTVVKMGRTQLQDAVPMTVGQEFGGWAVTLREDHDRLEESLKLVTEINLGATAIGTG
HHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCHHHCCC
LNAPEGYAEAARKHLAELTALPLSTSPHLVEATSDVGAFVHLSGVLKRVAVKLSKICSDL
CCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLSSGPRAGLNDIQLPAVQSGSSIMPGKVNPVIPEVVNQIAYQVVGYDATVTMAAEGGQ
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCE
LQLNAFEPVIMHSIGLSMKHLANGCSTLARKCIAGITVDEDALRRQVEQSIGLVTALNPA
EEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEECCHH
LGYTQSTMIAMEALHTGRGVAELVLERELLTEAELSELLRPERLANLAS
HCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCC
>Mature Secondary Structure 
TTTFSSTRTEHDLIGDAEVPAHVYWGVHTMRAAENFQITDQKLADNPFLIAALARVKQA
CCCCCCCCCHHHCCCCCCCCEEEEECHHHHHHCCCCEECHHHHCCCCHHHHHHHHHHHH
AARANLQLGLLDQTRAEAIDQACDRVAAGEFNEQFILDPIQGGAGTSTNMNANEVIANVA
HHHCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHH
LEILGHEKGDYQHLHPNDHVNLSQSTNDAYPTAVKIATCFAARPLLDALTELEQACRSKA
HHHHCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEFRTVVKMGRTQLQDAVPMTVGQEFGGWAVTLREDHDRLEESLKLVTEINLGATAIGTG
HHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCHHHCCC
LNAPEGYAEAARKHLAELTALPLSTSPHLVEATSDVGAFVHLSGVLKRVAVKLSKICSDL
CCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLSSGPRAGLNDIQLPAVQSGSSIMPGKVNPVIPEVVNQIAYQVVGYDATVTMAAEGGQ
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCE
LQLNAFEPVIMHSIGLSMKHLANGCSTLARKCIAGITVDEDALRRQVEQSIGLVTALNPA
EEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEECCHH
LGYTQSTMIAMEALHTGRGVAELVLERELLTEAELSELLRPERLANLAS
HCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3096982 [H]