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The map label for this gene is fadD21 [H]

Identifier: 145296861

GI number: 145296861

Start: 3061916

End: 3063778

Strand: Reverse

Name: fadD21 [H]

Synonym: cgR_2761

Alternate gene names: 145296861

Gene position: 3063778-3061916 (Counterclockwise)

Preceding gene: 145296862

Following gene: 145296860

Centisome position: 92.44

GC content: 56.95

Gene sequence:

>1863_bases
ATGGATTTAGATAAAGCGATTGGTTCATTCTTCGATGAAAATGGAGAAATCAACCTTCCTCCATTCCTAACTTTGGCAGC
CATGGGTGAGTTCATGTACCAGGCTGACATCGCTGAAGGCGGCGGGGATAAACCACGCATGCATTTCTGGGACTTCTCCG
AAGACCGCGATGGCAAGCTGATTCAGTACACCCGAAACGAGATCGATACTCGTATCAAGGCTGTAGCAGGCCGTTTGCAG
CAGGTCGCCACCCTGGGTGATCGTGCAGCGATCCTGGCGAACAACAGCCCTGAGTACATTTTCAGCTTCCTCGGCGCGAT
CTACGCTGGCATGGTCCCTGTGCCGCTTTATGATCCAAACGAGCCAGGACACGCAGACCACCTCAACGCTGTTTTCGCAG
ACAGCGAGCCAGTAGTTGTGCTGACCAACTCTAAGTCCGCAGGTGCCGTGCGCAAGCACTTCTCCAGCCTTCCAGCTGCA
GAACGCCCACGCATCCTCTCTGTAGATTCCTTGCCTGATTCCCTCGCAGATTCTTACGAGAACCCAATGCTGACCGAAGC
CGGCCGTCGCCTGGCTGCTCTGCGCCAGTCCGCACCTATCGATCTGACCGCATTCCTGCAGTACACCTCCGGTTCCACCC
GAACCCCAGCCGGCGTTGTTCTGACTAACCGCTCCATCTTGACCAACGTCTTGCAGATCTTCAGCGCCGCACAGCTGAAA
ACCCCACTGCGCCTGGTTTCATGGCTGCCACTGCACCACGACATGGGCATTATCCTCGCGGCGTTTGTCACCATGCTTGG
CCTGGACAACGAGTTCATGAACCCACGCGATTTCGTGCAGCAGCCTTCCCGCTGGATTAAGCAGCTCAACCGTCGCGAAA
GCGACGTGGACGTTAACGTCTACACCGTGGTTCCTAACTTCGCCCTCGAGCTTGCAGCACGCTACGCAAAGCCAGCAGAG
GGAGAGACCCTGGATCTTTCTGCACTGGATGCCATCATTATCGGTTCCGAGCCCGTCACCGAGAATGCACTGACCACCTT
CCGTGAAGCTTTCGAGCCTTACGGCCTGCCAGTTCAGACCCTGCGTCCTTCCTACGGTCTTGCAGAAGCATCCCTGCTGG
TAACCACCCCACAGACCGAAAACCGCCCACTGATCTCCTACTTCGACCGCGATGCCCTGGCTGAAAACCGCGTTGAGCTT
GTAGAAAAGAGCAATAACAAGGCTGTTGCTTTCGTCTCCAACGGCCAGGTTGCAGCCCCACAGCAGCTGGTCATCGTTGA
TTCCGAAACCGGAACCGAGCTGGCAGACGGCCAGATCGGCGAAATCTGGACCCACGGCGAAAACACCGCTGCAGGTTACC
TCGACCGCGAGGAAGACACCGCAGAAACCTTCCGCAACCGTCTGACCACCCGCCTGGAAGAAAACTCCCGCGCAGAAGGT
GCTGTCGACGACAACTACTGGATGGCCACCGGTGACCTCGGCGTCATCGTAGACAACGAGCTCTACATCACCGGTCGTCT
GAAGGACCTCATCGTTGTCGCAGGCCGAAACCACTACCCACAGGACATCGAGTACACCGTCCAGGCTGCTTCCGCACACA
TCCGTGCAGATTCCGTCGCAGCATTCGCAGTCCCAGGCGATGACATTGAAAAGCTCATCATCCTGGCAGAACGCGACACC
ACTGCAAACGAAGCCGACGATGCAGCTGCTGAAGAAGCAATCCGCTCCGCCGTTGGCACTGCACACGGTGTTGTTCCAGA
AGAGATCCGTATCCTCGCACCTGACGAGATCGCGCGTTCCTCCTCCGGAAAGATCGCACGCCGCGTCAACCAGCGCAACT
ACATTCAGGAACAAGCTAACTAG

Upstream 100 bases:

>100_bases
ACGGTCACTCGCTCAAGTAGTGTTAGTTTGCAAAAGTAATAAAATGTTCATCTTTGTCGATGGTCACAATAGTCATTAAA
GAATTGTAAGAGGGGTTCAC

Downstream 100 bases:

>100_bases
TTCTTTGCAGACACCGCAGGGCCCCGACGGATTTTCAACAGAATCCGTCGGGGTTCTTTTGGTTTTGTACACGGGCGATT
TGTCAGGTGAAGCTTCTCTA

Product: acyl-CoA synthase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MDLDKAIGSFFDENGEINLPPFLTLAAMGEFMYQADIAEGGGDKPRMHFWDFSEDRDGKLIQYTRNEIDTRIKAVAGRLQ
QVATLGDRAAILANNSPEYIFSFLGAIYAGMVPVPLYDPNEPGHADHLNAVFADSEPVVVLTNSKSAGAVRKHFSSLPAA
ERPRILSVDSLPDSLADSYENPMLTEAGRRLAALRQSAPIDLTAFLQYTSGSTRTPAGVVLTNRSILTNVLQIFSAAQLK
TPLRLVSWLPLHHDMGIILAAFVTMLGLDNEFMNPRDFVQQPSRWIKQLNRRESDVDVNVYTVVPNFALELAARYAKPAE
GETLDLSALDAIIIGSEPVTENALTTFREAFEPYGLPVQTLRPSYGLAEASLLVTTPQTENRPLISYFDRDALAENRVEL
VEKSNNKAVAFVSNGQVAAPQQLVIVDSETGTELADGQIGEIWTHGENTAAGYLDREEDTAETFRNRLTTRLEENSRAEG
AVDDNYWMATGDLGVIVDNELYITGRLKDLIVVAGRNHYPQDIEYTVQAASAHIRADSVAAFAVPGDDIEKLIILAERDT
TANEADDAAAEEAIRSAVGTAHGVVPEEIRILAPDEIARSSSGKIARRVNQRNYIQEQAN

Sequences:

>Translated_620_residues
MDLDKAIGSFFDENGEINLPPFLTLAAMGEFMYQADIAEGGGDKPRMHFWDFSEDRDGKLIQYTRNEIDTRIKAVAGRLQ
QVATLGDRAAILANNSPEYIFSFLGAIYAGMVPVPLYDPNEPGHADHLNAVFADSEPVVVLTNSKSAGAVRKHFSSLPAA
ERPRILSVDSLPDSLADSYENPMLTEAGRRLAALRQSAPIDLTAFLQYTSGSTRTPAGVVLTNRSILTNVLQIFSAAQLK
TPLRLVSWLPLHHDMGIILAAFVTMLGLDNEFMNPRDFVQQPSRWIKQLNRRESDVDVNVYTVVPNFALELAARYAKPAE
GETLDLSALDAIIIGSEPVTENALTTFREAFEPYGLPVQTLRPSYGLAEASLLVTTPQTENRPLISYFDRDALAENRVEL
VEKSNNKAVAFVSNGQVAAPQQLVIVDSETGTELADGQIGEIWTHGENTAAGYLDREEDTAETFRNRLTTRLEENSRAEG
AVDDNYWMATGDLGVIVDNELYITGRLKDLIVVAGRNHYPQDIEYTVQAASAHIRADSVAAFAVPGDDIEKLIILAERDT
TANEADDAAAEEAIRSAVGTAHGVVPEEIRILAPDEIARSSSGKIARRVNQRNYIQEQAN
>Mature_620_residues
MDLDKAIGSFFDENGEINLPPFLTLAAMGEFMYQADIAEGGGDKPRMHFWDFSEDRDGKLIQYTRNEIDTRIKAVAGRLQ
QVATLGDRAAILANNSPEYIFSFLGAIYAGMVPVPLYDPNEPGHADHLNAVFADSEPVVVLTNSKSAGAVRKHFSSLPAA
ERPRILSVDSLPDSLADSYENPMLTEAGRRLAALRQSAPIDLTAFLQYTSGSTRTPAGVVLTNRSILTNVLQIFSAAQLK
TPLRLVSWLPLHHDMGIILAAFVTMLGLDNEFMNPRDFVQQPSRWIKQLNRRESDVDVNVYTVVPNFALELAARYAKPAE
GETLDLSALDAIIIGSEPVTENALTTFREAFEPYGLPVQTLRPSYGLAEASLLVTTPQTENRPLISYFDRDALAENRVEL
VEKSNNKAVAFVSNGQVAAPQQLVIVDSETGTELADGQIGEIWTHGENTAAGYLDREEDTAETFRNRLTTRLEENSRAEG
AVDDNYWMATGDLGVIVDNELYITGRLKDLIVVAGRNHYPQDIEYTVQAASAHIRADSVAAFAVPGDDIEKLIILAERDT
TANEADDAAAEEAIRSAVGTAHGVVPEEIRILAPDEIARSSSGKIARRVNQRNYIQEQAN

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=530, Percent_Identity=22.6415094339623, Blast_Score=112, Evalue=2e-24,
Organism=Homo sapiens, GI225735629, Length=534, Percent_Identity=22.2846441947566, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI45827692, Length=534, Percent_Identity=22.2846441947566, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI55749758, Length=458, Percent_Identity=21.8340611353712, Blast_Score=101, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI193202679, Length=594, Percent_Identity=22.5589225589226, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=603, Percent_Identity=23.3830845771144, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24654656, Length=453, Percent_Identity=21.4128035320088, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 67960; Mature: 67960

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLDKAIGSFFDENGEINLPPFLTLAAMGEFMYQADIAEGGGDKPRMHFWDFSEDRDGKL
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCE
IQYTRNEIDTRIKAVAGRLQQVATLGDRAAILANNSPEYIFSFLGAIYAGMVPVPLYDPN
EEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCC
EPGHADHLNAVFADSEPVVVLTNSKSAGAVRKHFSSLPAAERPRILSVDSLPDSLADSYE
CCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHC
NPMLTEAGRRLAALRQSAPIDLTAFLQYTSGSTRTPAGVVLTNRSILTNVLQIFSAAQLK
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHC
TPLRLVSWLPLHHDMGIILAAFVTMLGLDNEFMNPRDFVQQPSRWIKQLNRRESDVDVNV
CHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEE
YTVVPNFALELAARYAKPAEGETLDLSALDAIIIGSEPVTENALTTFREAFEPYGLPVQT
EEECCHHHHHHHHHHCCCCCCCEEEHHHHCEEEECCCCCCHHHHHHHHHHHCCCCCCHHH
LRPSYGLAEASLLVTTPQTENRPLISYFDRDALAENRVELVEKSNNKAVAFVSNGQVAAP
CCCCCCCCCCEEEEECCCCCCCCHHHHHCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
QQLVIVDSETGTELADGQIGEIWTHGENTAAGYLDREEDTAETFRNRLTTRLEENSRAEG
CEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AVDDNYWMATGDLGVIVDNELYITGRLKDLIVVAGRNHYPQDIEYTVQAASAHIRADSVA
CCCCCEEEEECCEEEEECCEEEEEECCEEEEEEECCCCCCCCHHHHEEHHHHEEECCCEE
AFAVPGDDIEKLIILAERDTTANEADDAAAEEAIRSAVGTAHGVVPEEIRILAPDEIARS
EEECCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHEEEECCHHHHCC
SSGKIARRVNQRNYIQEQAN
CCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLDKAIGSFFDENGEINLPPFLTLAAMGEFMYQADIAEGGGDKPRMHFWDFSEDRDGKL
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCE
IQYTRNEIDTRIKAVAGRLQQVATLGDRAAILANNSPEYIFSFLGAIYAGMVPVPLYDPN
EEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCC
EPGHADHLNAVFADSEPVVVLTNSKSAGAVRKHFSSLPAAERPRILSVDSLPDSLADSYE
CCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHC
NPMLTEAGRRLAALRQSAPIDLTAFLQYTSGSTRTPAGVVLTNRSILTNVLQIFSAAQLK
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHC
TPLRLVSWLPLHHDMGIILAAFVTMLGLDNEFMNPRDFVQQPSRWIKQLNRRESDVDVNV
CHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEE
YTVVPNFALELAARYAKPAEGETLDLSALDAIIIGSEPVTENALTTFREAFEPYGLPVQT
EEECCHHHHHHHHHHCCCCCCCEEEHHHHCEEEECCCCCCHHHHHHHHHHHCCCCCCHHH
LRPSYGLAEASLLVTTPQTENRPLISYFDRDALAENRVELVEKSNNKAVAFVSNGQVAAP
CCCCCCCCCCEEEEECCCCCCCCHHHHHCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
QQLVIVDSETGTELADGQIGEIWTHGENTAAGYLDREEDTAETFRNRLTTRLEENSRAEG
CEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AVDDNYWMATGDLGVIVDNELYITGRLKDLIVVAGRNHYPQDIEYTVQAASAHIRADSVA
CCCCCEEEEECCEEEEECCEEEEEECCEEEEEEECCCCCCCCHHHHEEHHHHEEECCCEE
AFAVPGDDIEKLIILAERDTTANEADDAAAEEAIRSAVGTAHGVVPEEIRILAPDEIARS
EEECCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHEEEECCHHHHCC
SSGKIARRVNQRNYIQEQAN
CCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]