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The map label for this gene is trmB

Identifier: 145296853

GI number: 145296853

Start: 3049666

End: 3050433

Strand: Direct

Name: trmB

Synonym: cgR_2753

Alternate gene names: 145296853

Gene position: 3049666-3050433 (Clockwise)

Preceding gene: 145296852

Following gene: 145296854

Centisome position: 92.02

GC content: 53.91

Gene sequence:

>768_bases
ATGTCTATTTCTGATAATTCCCGCGATCAATTAGGAGAACTGCCAGCTGGTCGGCCTCTCCAATCCGATTTTGATAATGA
CCTCGACTACCCACGTCTAGGCAGTGTCACGTTTAGGCGTGGCACCCTCACTGAAAACCAGCAAACCATGTGGGATGAAA
AGTGGCCTGAACTGGGTCGCGTCCTCGAAGATGAGCTGATTGATGTTGATGCGTGGTTCGGGCGCGAAGGCGCAAAAACC
ATCGTAGAGATCGGCTCTGGCACTGGAACTTCTACTGCTGCCATGGCTCCACTTGAGGCTGATACCAACATTGTCGCCGT
CGAACTATACAAGCCGGGCTTGGCCAAGTTGATGGGCTCTGTTGTCCGTGGAGAGATCGACAACGTGCGCATGGTCCGCG
GAGACGGCATCGAGGTGCTCAACCGCATGTTTGCCGATGGGTCCCTGGACGGCATCCGCGTATACTTCCCGGACCCTTGG
CCAAAGGCGCGCCACAACAAGCGCCGCATCATCCAGTCTGGTCCGCTGAACCTGTTTGCAAAGAAGCTCAAGCCAGGTGG
AGTTCTGCACGTTGCTACCGACCACGCTGATTACGCAGAGTGGATCAATGAGCTAGTTGAGGTCGAACCACTGCTTGAGT
ACAAAGGCTGGCCATGGGAGGAATGCCCTCAGCTGACTGACCGTCAGGTCATCACCAAGTTTGAAGGCAAAGGCTTGGAA
AAAGATCACGTGATCAATGAGTACTTGTGGCAGAAGGTGCAAAACTAA

Upstream 100 bases:

>100_bases
GCCTAAATTGCAGCGAGAGGTCTAAAAGGTAGTGTTCGAGGGATTCATCCAAACTCACGAATATTGAAGTTTTAAAGTTG
AACAGGAAAAATAACAAGTA

Downstream 100 bases:

>100_bases
TGTCTGATGTGCATGAGGTCATTCATAGCTACAATCCCACTGACAAAGAAGGCCCTGAAAGTGTCCTTCTAGTGTGGGAC
GCTCCCAACCTAGATATGGG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSISDNSRDQLGELPAGRPLQSDFDNDLDYPRLGSVTFRRGTLTENQQTMWDEKWPELGRVLEDELIDVDAWFGREGAKT
IVEIGSGTGTSTAAMAPLEADTNIVAVELYKPGLAKLMGSVVRGEIDNVRMVRGDGIEVLNRMFADGSLDGIRVYFPDPW
PKARHNKRRIIQSGPLNLFAKKLKPGGVLHVATDHADYAEWINELVEVEPLLEYKGWPWEECPQLTDRQVITKFEGKGLE
KDHVINEYLWQKVQN

Sequences:

>Translated_255_residues
MSISDNSRDQLGELPAGRPLQSDFDNDLDYPRLGSVTFRRGTLTENQQTMWDEKWPELGRVLEDELIDVDAWFGREGAKT
IVEIGSGTGTSTAAMAPLEADTNIVAVELYKPGLAKLMGSVVRGEIDNVRMVRGDGIEVLNRMFADGSLDGIRVYFPDPW
PKARHNKRRIIQSGPLNLFAKKLKPGGVLHVATDHADYAEWINELVEVEPLLEYKGWPWEECPQLTDRQVITKFEGKGLE
KDHVINEYLWQKVQN
>Mature_254_residues
SISDNSRDQLGELPAGRPLQSDFDNDLDYPRLGSVTFRRGTLTENQQTMWDEKWPELGRVLEDELIDVDAWFGREGAKTI
VEIGSGTGTSTAAMAPLEADTNIVAVELYKPGLAKLMGSVVRGEIDNVRMVRGDGIEVLNRMFADGSLDGIRVYFPDPWP
KARHNKRRIIQSGPLNLFAKKLKPGGVLHVATDHADYAEWINELVEVEPLLEYKGWPWEECPQLTDRQVITKFEGKGLEK
DHVINEYLWQKVQN

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=238, Percent_Identity=34.8739495798319, Blast_Score=142, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320000, Length=170, Percent_Identity=29.4117647058824, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_CORGB (A4QHQ6)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139674.1
- ProteinModelPortal:   A4QHQ6
- STRING:   A4QHQ6
- GeneID:   4994270
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2753
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   YAEWIDE
- ProtClustDB:   PRK00121
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 28806; Mature: 28675

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: BINDING 83-83 BINDING 108-108 BINDING 135-135 BINDING 158-158 BINDING 162-162 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISDNSRDQLGELPAGRPLQSDFDNDLDYPRLGSVTFRRGTLTENQQTMWDEKWPELGR
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
VLEDELIDVDAWFGREGAKTIVEIGSGTGTSTAAMAPLEADTNIVAVELYKPGLAKLMGS
HHHHHHHCHHHHHCCCCCCEEEEECCCCCCCHHEECCCCCCCCEEEEEECCCCHHHHHHH
VVRGEIDNVRMVRGDGIEVLNRMFADGSLDGIRVYFPDPWPKARHNKRRIIQSGPLNLFA
HHHCCCCCEEEECCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCHHHHH
KKLKPGGVLHVATDHADYAEWINELVEVEPLLEYKGWPWEECPQLTDRQVITKFEGKGLE
HHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHCCCCCC
KDHVINEYLWQKVQN
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SISDNSRDQLGELPAGRPLQSDFDNDLDYPRLGSVTFRRGTLTENQQTMWDEKWPELGR
CCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
VLEDELIDVDAWFGREGAKTIVEIGSGTGTSTAAMAPLEADTNIVAVELYKPGLAKLMGS
HHHHHHHCHHHHHCCCCCCEEEEECCCCCCCHHEECCCCCCCCEEEEEECCCCHHHHHHH
VVRGEIDNVRMVRGDGIEVLNRMFADGSLDGIRVYFPDPWPKARHNKRRIIQSGPLNLFA
HHHCCCCCEEEECCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCHHHHH
KKLKPGGVLHVATDHADYAEWINELVEVEPLLEYKGWPWEECPQLTDRQVITKFEGKGLE
HHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHCCCCCC
KDHVINEYLWQKVQN
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA