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The map label for this gene is def2 [H]

Identifier: 145296738

GI number: 145296738

Start: 2923434

End: 2924015

Strand: Direct

Name: def2 [H]

Synonym: cgR_2642

Alternate gene names: 145296738

Gene position: 2923434-2924015 (Clockwise)

Preceding gene: 145296733

Following gene: 145296739

Centisome position: 88.21

GC content: 51.89

Gene sequence:

>582_bases
ATGACTGTCCGACCAATCGTTATTCATGGAGATCCTGTTCTCCACAACCCTACCCAGCTTGTTACTGAGGATGTCTCTGA
ACTGCAGGAACTAATTGCAGATATGTACGAGACGATGGATGTCGCCAATGGTGTGGGTCTTGCGGCCAACCAGATTGGTG
TGTCCAAGCGCATTTTTGTTTATGACTGTCCTGATGATGAGGGCGTGATGCACAAGGGTTGTTTCATCAATCCTGTGTTG
GAAACCTCTGAAATCCCAGAGACCATGCCTGCCGATGATGGCTCCGACGAGGAAGGCTGCCTGTCTGTTCCTGGCGAGGG
CTTCCCCACTGGCCGTGCTCATTGGGCGAAGGTTACTGGACTGAATGAAAAGGGCGAGGAAGTTTCTGTTGAGGCTGAGG
GTTTCTTGGCTCGTTGCTTCCAGCATGAGGTTGGCCACCTTGATGGTTTCTTGTACACCGATGTGTTGATTGGTCGGTGG
AAGCGCATGGCTAAGAAAGCTATTAAGGCCAATGGGTGGACTGAGCCTGGTTTGACCTGGATGCCGGGTGAAGATGAGGA
TCCTTTCGGGCATGACACCTAG

Upstream 100 bases:

>100_bases
CGCGAATGGCTTCCTCTTTAGCCCCGATTGCGTGGGGTGCGTGGGCCTCAAACTCGAGGAGGGTTAAATCATCTGCGGAA
AGCATGCTTAGAATGTTGCC

Downstream 100 bases:

>100_bases
TCTTCCCCGTTTCCGCAGCCAGAAACCTGCCGTCGGCGATCGTGTTGTTGCACGTCGCCGGATTCCTGGTGCCAATGTGC
ATTGGACAGATGTCATTGGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MTVRPIVIHGDPVLHNPTQLVTEDVSELQELIADMYETMDVANGVGLAANQIGVSKRIFVYDCPDDEGVMHKGCFINPVL
ETSEIPETMPADDGSDEEGCLSVPGEGFPTGRAHWAKVTGLNEKGEEVSVEAEGFLARCFQHEVGHLDGFLYTDVLIGRW
KRMAKKAIKANGWTEPGLTWMPGEDEDPFGHDT

Sequences:

>Translated_193_residues
MTVRPIVIHGDPVLHNPTQLVTEDVSELQELIADMYETMDVANGVGLAANQIGVSKRIFVYDCPDDEGVMHKGCFINPVL
ETSEIPETMPADDGSDEEGCLSVPGEGFPTGRAHWAKVTGLNEKGEEVSVEAEGFLARCFQHEVGHLDGFLYTDVLIGRW
KRMAKKAIKANGWTEPGLTWMPGEDEDPFGHDT
>Mature_192_residues
TVRPIVIHGDPVLHNPTQLVTEDVSELQELIADMYETMDVANGVGLAANQIGVSKRIFVYDCPDDEGVMHKGCFINPVLE
TSEIPETMPADDGSDEEGCLSVPGEGFPTGRAHWAKVTGLNEKGEEVSVEAEGFLARCFQHEVGHLDGFLYTDVLIGRWK
RMAKKAIKANGWTEPGLTWMPGEDEDPFGHDT

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=157, Percent_Identity=35.6687898089172, Blast_Score=92, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24645726, Length=162, Percent_Identity=32.7160493827161, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24645728, Length=164, Percent_Identity=29.2682926829268, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 21169; Mature: 21038

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRPIVIHGDPVLHNPTQLVTEDVSELQELIADMYETMDVANGVGLAANQIGVSKRIFV
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEEE
YDCPDDEGVMHKGCFINPVLETSEIPETMPADDGSDEEGCLSVPGEGFPTGRAHWAKVTG
EECCCCCCCEECCCEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEECC
LNEKGEEVSVEAEGFLARCFQHEVGHLDGFLYTDVLIGRWKRMAKKAIKANGWTEPGLTW
CCCCCCEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
MPGEDEDPFGHDT
CCCCCCCCCCCCC
>Mature Secondary Structure 
TVRPIVIHGDPVLHNPTQLVTEDVSELQELIADMYETMDVANGVGLAANQIGVSKRIFV
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEEE
YDCPDDEGVMHKGCFINPVLETSEIPETMPADDGSDEEGCLSVPGEGFPTGRAHWAKVTG
EECCCCCCCEECCCEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEECC
LNEKGEEVSVEAEGFLARCFQHEVGHLDGFLYTDVLIGRWKRMAKKAIKANGWTEPGLTW
CCCCCCEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
MPGEDEDPFGHDT
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]