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The map label for this gene is thcD [H]

Identifier: 145296706

GI number: 145296706

Start: 2882221

End: 2883456

Strand: Reverse

Name: thcD [H]

Synonym: cgR_2611

Alternate gene names: 145296706

Gene position: 2883456-2882221 (Counterclockwise)

Preceding gene: 145296710

Following gene: 145296705

Centisome position: 87.0

GC content: 53.88

Gene sequence:

>1236_bases
ATGAGCACACCACAAAGCATCGTCATCATCGGCGGCGGTTTAGCCGGAGCGAAAACCGCAGAGGCACTGCGTGTAAACGG
GCATGAAGGCTCCATCACGCTCATCGCAGCAGAAAATCATCTGCCATATGAACGCCCACCGCTGTCAAAGGAGTACATGG
CGGGAAAAGTAGGCTTTGAAAAGGCGATTGTTCACCCGGCGGGGTGGTACAAAGAAAACAATGTCACGCTGCGTCAAGGT
GTGCGTGCAACGGCAATTGATGCGGGTTCACGCCAAGTCACCGTTGATGATGGCGGAAACACTGAGACCATTAACTACGA
CAAACTAGTTCTTGCTACTGGCTCAGCAGTGCGCAAACTTCCAATTCCGGGAGCCGACGCCTCTAATGTGCACTACCTGC
GCGCCGTGGAAGACTCTGACGCGATCAAGGCAACCTTCGGTGAAGGTAAAAAGCTGGTCCTCATCGGTGGTGGCTGGATC
GGACTCGAAGTCGCATCAGCGGCACGAGGAGCTGGCACTGACGTCACTGTTTTGGAAGGTGGAAAGCTCCCACTTTTGAA
CGTCCTTGGTGAAACGGTCGCGCAAGTCTTTGCCGATCTGCATGTGGCAAACGGCGTTGACCTGCGCACCGAAGTGAAAA
TTACGGACATCGTCACCGAAGATGGACGTGCAGTTGGCGTGCGACTTGATGACGGCGAAGTGGTTCCCGCAGACGCAGTA
GTCATTGGTATCGGTGTCACCCCAGTGATTGACCTGGCAGAATCTGCTGGCCTAGAAATCGACAATGGTGTTTTGGTGGA
CGCAGCACTGCGTACCAGCGACCCGGATATCTACGCAGTTGGAGACATCGCGAACCACGATCACCCAGTTCTAGGACACC
GCATCCGCGTGGAGCACTGGGCCACCGCGTTGAATCAACCTGCGGCTGCGGTGAAATCCCTACTTGGCAAAGACGCCGAG
TTCACCAACCTTCCGTACTTCTTTACAGATCAATTCGATCTGGGTTGTGAATACGTCGGCCACGCCACCGGTTCGCAGGA
GAAGGTATTCATCCGTGGAAACCTTGAAACACGAGAATTTGTCGCCTTCTGGGTTGATGCTGAAAACCGAATTCTCGCCG
CAATGAACGTGAATGTGTGGGATGTTCCTGATCAAATCAAGCCTCTCATCGCATCAGGAAAGAGCGTTGACACCGAGAGG
CTAGTGGATCCAGAAGTTCCGTATTCAGAGCTCTAA

Upstream 100 bases:

>100_bases
CGCTCGCCGAGGGTTTCGGCCTTTTAACTAGCATGGCGTATAGATCCATGCGGTCGACCTTGCGCCTGACCGTCCACTTT
TTAGGGACTAGGAGTACAGC

Downstream 100 bases:

>100_bases
GCAGTGTGTTTGATTGCCGCGCTGCCCTTTTGCAAAGAGGGGAGGCGTGGCCTTTTCTTGTCTGGGGCGGAGTGGGTTCG
AAATCACTCTTGCATTTTTC

Product: hypothetical protein

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MSTPQSIVIIGGGLAGAKTAEALRVNGHEGSITLIAAENHLPYERPPLSKEYMAGKVGFEKAIVHPAGWYKENNVTLRQG
VRATAIDAGSRQVTVDDGGNTETINYDKLVLATGSAVRKLPIPGADASNVHYLRAVEDSDAIKATFGEGKKLVLIGGGWI
GLEVASAARGAGTDVTVLEGGKLPLLNVLGETVAQVFADLHVANGVDLRTEVKITDIVTEDGRAVGVRLDDGEVVPADAV
VIGIGVTPVIDLAESAGLEIDNGVLVDAALRTSDPDIYAVGDIANHDHPVLGHRIRVEHWATALNQPAAAVKSLLGKDAE
FTNLPYFFTDQFDLGCEYVGHATGSQEKVFIRGNLETREFVAFWVDAENRILAAMNVNVWDVPDQIKPLIASGKSVDTER
LVDPEVPYSEL

Sequences:

>Translated_411_residues
MSTPQSIVIIGGGLAGAKTAEALRVNGHEGSITLIAAENHLPYERPPLSKEYMAGKVGFEKAIVHPAGWYKENNVTLRQG
VRATAIDAGSRQVTVDDGGNTETINYDKLVLATGSAVRKLPIPGADASNVHYLRAVEDSDAIKATFGEGKKLVLIGGGWI
GLEVASAARGAGTDVTVLEGGKLPLLNVLGETVAQVFADLHVANGVDLRTEVKITDIVTEDGRAVGVRLDDGEVVPADAV
VIGIGVTPVIDLAESAGLEIDNGVLVDAALRTSDPDIYAVGDIANHDHPVLGHRIRVEHWATALNQPAAAVKSLLGKDAE
FTNLPYFFTDQFDLGCEYVGHATGSQEKVFIRGNLETREFVAFWVDAENRILAAMNVNVWDVPDQIKPLIASGKSVDTER
LVDPEVPYSEL
>Mature_410_residues
STPQSIVIIGGGLAGAKTAEALRVNGHEGSITLIAAENHLPYERPPLSKEYMAGKVGFEKAIVHPAGWYKENNVTLRQGV
RATAIDAGSRQVTVDDGGNTETINYDKLVLATGSAVRKLPIPGADASNVHYLRAVEDSDAIKATFGEGKKLVLIGGGWIG
LEVASAARGAGTDVTVLEGGKLPLLNVLGETVAQVFADLHVANGVDLRTEVKITDIVTEDGRAVGVRLDDGEVVPADAVV
IGIGVTPVIDLAESAGLEIDNGVLVDAALRTSDPDIYAVGDIANHDHPVLGHRIRVEHWATALNQPAAAVKSLLGKDAEF
TNLPYFFTDQFDLGCEYVGHATGSQEKVFIRGNLETREFVAFWVDAENRILAAMNVNVWDVPDQIKPLIASGKSVDTERL
VDPEVPYSEL

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=398, Percent_Identity=27.8894472361809, Blast_Score=185, Evalue=9e-47,
Organism=Homo sapiens, GI226437568, Length=398, Percent_Identity=27.8894472361809, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI65787454, Length=398, Percent_Identity=27.8894472361809, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI4757732, Length=347, Percent_Identity=28.5302593659942, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI22202629, Length=347, Percent_Identity=28.5302593659942, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1788892, Length=410, Percent_Identity=33.4146341463415, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1789065, Length=323, Percent_Identity=30.030959752322, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1789765, Length=291, Percent_Identity=30.2405498281787, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87082354, Length=221, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786307, Length=218, Percent_Identity=30.2752293577982, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=375, Percent_Identity=32.2666666666667, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI32564386, Length=338, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24639257, Length=402, Percent_Identity=30.3482587064677, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI281359715, Length=402, Percent_Identity=30.3482587064677, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI281359713, Length=402, Percent_Identity=30.3482587064677, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24639250, Length=402, Percent_Identity=30.3482587064677, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI18543267, Length=402, Percent_Identity=30.3482587064677, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24639252, Length=402, Percent_Identity=30.3482587064677, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24585130, Length=408, Percent_Identity=28.1862745098039, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24581020, Length=360, Percent_Identity=26.3888888888889, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI28573993, Length=360, Percent_Identity=26.3888888888889, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43648; Mature: 43517

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPQSIVIIGGGLAGAKTAEALRVNGHEGSITLIAAENHLPYERPPLSKEYMAGKVGFE
CCCCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCH
KAIVHPAGWYKENNVTLRQGVRATAIDAGSRQVTVDDGGNTETINYDKLVLATGSAVRKL
HEEECCCCCEECCCEEHHCCCEEEEECCCCEEEEECCCCCEEEEECCEEEEEECCCEEEC
PIPGADASNVHYLRAVEDSDAIKATFGEGKKLVLIGGGWIGLEVASAARGAGTDVTVLEG
CCCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEECCCEEEEEEHHHCCCCCCEEEEEEC
GKLPLLNVLGETVAQVFADLHVANGVDLRTEVKITDIVTEDGRAVGVRLDDGEVVPADAV
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCCEE
VIGIGVTPVIDLAESAGLEIDNGVLVDAALRTSDPDIYAVGDIANHDHPVLGHRIRVEHW
EEECCCCHHHHHHHHCCCEECCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEHHHH
ATALNQPAAAVKSLLGKDAEFTNLPYFFTDQFDLGCEYVGHATGSQEKVFIRGNLETREF
HHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCEEEEEECCCCEEE
VAFWVDAENRILAAMNVNVWDVPDQIKPLIASGKSVDTERLVDPEVPYSEL
EEEEECCCCCEEEEEECEEECCCHHHHHHHHCCCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
STPQSIVIIGGGLAGAKTAEALRVNGHEGSITLIAAENHLPYERPPLSKEYMAGKVGFE
CCCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHCCCCCH
KAIVHPAGWYKENNVTLRQGVRATAIDAGSRQVTVDDGGNTETINYDKLVLATGSAVRKL
HEEECCCCCEECCCEEHHCCCEEEEECCCCEEEEECCCCCEEEEECCEEEEEECCCEEEC
PIPGADASNVHYLRAVEDSDAIKATFGEGKKLVLIGGGWIGLEVASAARGAGTDVTVLEG
CCCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEECCCEEEEEEHHHCCCCCCEEEEEEC
GKLPLLNVLGETVAQVFADLHVANGVDLRTEVKITDIVTEDGRAVGVRLDDGEVVPADAV
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCCEE
VIGIGVTPVIDLAESAGLEIDNGVLVDAALRTSDPDIYAVGDIANHDHPVLGHRIRVEHW
EEECCCCHHHHHHHHCCCEECCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEHHHH
ATALNQPAAAVKSLLGKDAEFTNLPYFFTDQFDLGCEYVGHATGSQEKVFIRGNLETREF
HHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCEEEEEECCCCEEE
VAFWVDAENRILAAMNVNVWDVPDQIKPLIASGKSVDTERLVDPEVPYSEL
EEEEECCCCCEEEEEECEEECCCHHHHHHHHCCCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]