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The map label for this gene is kstD [H]

Identifier: 145296612

GI number: 145296612

Start: 2785510

End: 2787183

Strand: Reverse

Name: kstD [H]

Synonym: cgR_2520

Alternate gene names: 145296612

Gene position: 2787183-2785510 (Counterclockwise)

Preceding gene: 145296613

Following gene: 145296611

Centisome position: 84.1

GC content: 59.38

Gene sequence:

>1674_bases
ATGTCTAAGCCACTTCTTTCCTCAGTTGCCGATTGGCCTGAAGAATTAGATGTCCTCGTGCTTGGGTCTGGTTGTGCTGG
AATGACCGCCGCACTTGTCGCGGCGGATGGAGGCGCGCTGGTGGGCGTCGTCGAAAAGCATGACAAGCTTGGTGGCACGA
CGGCCGCAGGTGGCGGAGTGATGTGGGCGCCGAACAACCATCTCATGGGGCAGCGGGGTTTCACCGACTCCGAGGAAGCT
GCTGCGGAGTACATTCGTGCGGCGACCAAGGGCAAGTTGTCCGACGAGGAAATTGCCTGGTACATCGAGAATTCCGCTGA
GGCAGTGCTGTTTTTGGATGAGAAAACCCGCGTGGATTACGCGCCACTGAATCGCCCGGATTACCACCGCGAGTGGCCAG
GCTCCACCGAAGGCGGCCGCGGTTTGGACCACAACCCGTTTGACCCCTCCGTGGTTCCCGGCCTGGTTGAGGCCGTGCGC
CAGCCGACCTACCTGCCGCTGATCACCATGGATGAGCGCGACCACCTCCACGGCGCCGCACCTGATCCGCAGCTGCTTAA
AGACCGCGCGAACCAGGGCGTACGCACCATGGGTGGCGCATTATCCTCCGCCATGATCGCGTCCGCCTGGGACGCCGGCA
TCCGCATCGCAGCTGGCACCGCAGCCAGCTCCCTCCAGCGCACCGAGTCCGGCTGGGAAGTTTTGCTTATCGACGCCTCC
CTCCAGCGCACCATCACCGCAAAGCACGTGGTCATCGCCTCAGGTGGCTTCGAATGGTCCAAGGAGCTCACCTCGACCCT
CCTGAAGTTCCCGATCACTCCAATTTCTGCACCATCTAACACCGGCGATGGCCTTAAGCTGGGCATCGGCGTGGGCGCTG
CGTTCACAGAGACAACCGATATCTGGGGCGTTCCTGTTATCACCGCACACGGCGCGGAATACGATGGCCAGCAATCTGGA
CGCATGGGCAACGTGGAGGCAACACTGCCTGGCTCCATCGTGGTGAACAAGCTGGGTAAACGCTTTGTTAATGAAGCTTT
GAACTACCACGATTTCTCTCGAGTCTTTGCCAACATCGACCCAGAGGAATCAGATTTTGCCAACATCCCGGCGTACCTAG
TGATGGATTCCTCCTTCACCTCTAAGTACCCAGTGGCAGGTAACTCAACTTTTACCTCCGGCGCGGAAGCACCAGCATGG
ATGGTTCAGGCAGATACTCTGGAAGAACTCGCTGCGAAACTCGGCGTGGATGCTGCAGGCCTTGCAAGCACCGTTTCACG
ATTCAATGACGATGCCCGCACTGGTGTCGACTCCGAATTCGGCCGCGGCGCAACAGAGCAGGACCGCCACCTTGGCGATC
CAGCCAACACCCCAAACCCATGTTTGGCACCTTTAGAAAGTGGCCCTTTCTACGCCATCGAACTCCACCCCGGAGTCCTG
GGCACAGCTGGTGGATTGCAGACCGACCTAGACGGCGAAGTCCTCGGCTGGGATGGCCTTCCACTCAGCGGACTGTATGC
GGCAGGTAACTGCTCTGCAACCGTATTCAAGGACGCCTACCCAGGTGGCGGCGCAACCATCGGATCCGCCATCACCCGCG
CATACGCAGTGGGTAAGCACATCAGCCAATCCTTTCAGATGGAAACCACTGAAGCACTGAGCTTGACTCACTAA

Upstream 100 bases:

>100_bases
CACCGTTTTGATTTTGACAATCTCGATGAGGCGTTCACTACTTTGACCACACCAAACGCTGGTGTGGGAAAGATCGCCAT
TGATTTCCCAGGAGCTCAAA

Downstream 100 bases:

>100_bases
GAAACAAAAGTAGAAGGAACAGAGAAGAACAATGGTGGATAAAGCTTCCGAAGCGAAGTGGGACAAAGAAGTTGACCTGC
TTGTTCTAGGATCAGGCGCA

Product: hypothetical protein

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MSKPLLSSVADWPEELDVLVLGSGCAGMTAALVAADGGALVGVVEKHDKLGGTTAAGGGVMWAPNNHLMGQRGFTDSEEA
AAEYIRAATKGKLSDEEIAWYIENSAEAVLFLDEKTRVDYAPLNRPDYHREWPGSTEGGRGLDHNPFDPSVVPGLVEAVR
QPTYLPLITMDERDHLHGAAPDPQLLKDRANQGVRTMGGALSSAMIASAWDAGIRIAAGTAASSLQRTESGWEVLLIDAS
LQRTITAKHVVIASGGFEWSKELTSTLLKFPITPISAPSNTGDGLKLGIGVGAAFTETTDIWGVPVITAHGAEYDGQQSG
RMGNVEATLPGSIVVNKLGKRFVNEALNYHDFSRVFANIDPEESDFANIPAYLVMDSSFTSKYPVAGNSTFTSGAEAPAW
MVQADTLEELAAKLGVDAAGLASTVSRFNDDARTGVDSEFGRGATEQDRHLGDPANTPNPCLAPLESGPFYAIELHPGVL
GTAGGLQTDLDGEVLGWDGLPLSGLYAAGNCSATVFKDAYPGGGATIGSAITRAYAVGKHISQSFQMETTEALSLTH

Sequences:

>Translated_557_residues
MSKPLLSSVADWPEELDVLVLGSGCAGMTAALVAADGGALVGVVEKHDKLGGTTAAGGGVMWAPNNHLMGQRGFTDSEEA
AAEYIRAATKGKLSDEEIAWYIENSAEAVLFLDEKTRVDYAPLNRPDYHREWPGSTEGGRGLDHNPFDPSVVPGLVEAVR
QPTYLPLITMDERDHLHGAAPDPQLLKDRANQGVRTMGGALSSAMIASAWDAGIRIAAGTAASSLQRTESGWEVLLIDAS
LQRTITAKHVVIASGGFEWSKELTSTLLKFPITPISAPSNTGDGLKLGIGVGAAFTETTDIWGVPVITAHGAEYDGQQSG
RMGNVEATLPGSIVVNKLGKRFVNEALNYHDFSRVFANIDPEESDFANIPAYLVMDSSFTSKYPVAGNSTFTSGAEAPAW
MVQADTLEELAAKLGVDAAGLASTVSRFNDDARTGVDSEFGRGATEQDRHLGDPANTPNPCLAPLESGPFYAIELHPGVL
GTAGGLQTDLDGEVLGWDGLPLSGLYAAGNCSATVFKDAYPGGGATIGSAITRAYAVGKHISQSFQMETTEALSLTH
>Mature_556_residues
SKPLLSSVADWPEELDVLVLGSGCAGMTAALVAADGGALVGVVEKHDKLGGTTAAGGGVMWAPNNHLMGQRGFTDSEEAA
AEYIRAATKGKLSDEEIAWYIENSAEAVLFLDEKTRVDYAPLNRPDYHREWPGSTEGGRGLDHNPFDPSVVPGLVEAVRQ
PTYLPLITMDERDHLHGAAPDPQLLKDRANQGVRTMGGALSSAMIASAWDAGIRIAAGTAASSLQRTESGWEVLLIDASL
QRTITAKHVVIASGGFEWSKELTSTLLKFPITPISAPSNTGDGLKLGIGVGAAFTETTDIWGVPVITAHGAEYDGQQSGR
MGNVEATLPGSIVVNKLGKRFVNEALNYHDFSRVFANIDPEESDFANIPAYLVMDSSFTSKYPVAGNSTFTSGAEAPAWM
VQADTLEELAAKLGVDAAGLASTVSRFNDDARTGVDSEFGRGATEQDRHLGDPANTPNPCLAPLESGPFYAIELHPGVLG
TAGGLQTDLDGEVLGWDGLPLSGLYAAGNCSATVFKDAYPGGGATIGSAITRAYAVGKHISQSFQMETTEALSLTH

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 58504; Mature: 58373

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPLLSSVADWPEELDVLVLGSGCAGMTAALVAADGGALVGVVEKHDKLGGTTAAGGGV
CCCCHHHHHHCCHHHCCEEEECCCCCCHHEEEEECCCCEEEEEEHHHHHCCCCCCCCCCE
MWAPNNHLMGQRGFTDSEEAAAEYIRAATKGKLSDEEIAWYIENSAEAVLFLDEKTRVDY
EECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCC
APLNRPDYHREWPGSTEGGRGLDHNPFDPSVVPGLVEAVRQPTYLPLITMDERDHLHGAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
PDPQLLKDRANQGVRTMGGALSSAMIASAWDAGIRIAAGTAASSLQRTESGWEVLLIDAS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCEEEEEECC
LQRTITAKHVVIASGGFEWSKELTSTLLKFPITPISAPSNTGDGLKLGIGVGAAFTETTD
HHHHEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEECCCCC
IWGVPVITAHGAEYDGQQSGRMGNVEATLPGSIVVNKLGKRFVNEALNYHDFSRVFANID
CCCCCEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCC
PEESDFANIPAYLVMDSSFTSKYPVAGNSTFTSGAEAPAWMVQADTLEELAAKLGVDAAG
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCHHH
LASTVSRFNDDARTGVDSEFGRGATEQDRHLGDPANTPNPCLAPLESGPFYAIELHPGVL
HHHHHHHHCCHHHCCCCHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCE
GTAGGLQTDLDGEVLGWDGLPLSGLYAAGNCSATVFKDAYPGGGATIGSAITRAYAVGKH
ECCCCCCCCCCCCEECCCCCCCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
ISQSFQMETTEALSLTH
HHHHHCCCHHHHHCCCC
>Mature Secondary Structure 
SKPLLSSVADWPEELDVLVLGSGCAGMTAALVAADGGALVGVVEKHDKLGGTTAAGGGV
CCCHHHHHHCCHHHCCEEEECCCCCCHHEEEEECCCCEEEEEEHHHHHCCCCCCCCCCE
MWAPNNHLMGQRGFTDSEEAAAEYIRAATKGKLSDEEIAWYIENSAEAVLFLDEKTRVDY
EECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCC
APLNRPDYHREWPGSTEGGRGLDHNPFDPSVVPGLVEAVRQPTYLPLITMDERDHLHGAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
PDPQLLKDRANQGVRTMGGALSSAMIASAWDAGIRIAAGTAASSLQRTESGWEVLLIDAS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCEEEEEECC
LQRTITAKHVVIASGGFEWSKELTSTLLKFPITPISAPSNTGDGLKLGIGVGAAFTETTD
HHHHEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEECCCCC
IWGVPVITAHGAEYDGQQSGRMGNVEATLPGSIVVNKLGKRFVNEALNYHDFSRVFANID
CCCCCEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCC
PEESDFANIPAYLVMDSSFTSKYPVAGNSTFTSGAEAPAWMVQADTLEELAAKLGVDAAG
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCHHH
LASTVSRFNDDARTGVDSEFGRGATEQDRHLGDPANTPNPCLAPLESGPFYAIELHPGVL
HHHHHHHHCCHHHCCCCHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCE
GTAGGLQTDLDGEVLGWDGLPLSGLYAAGNCSATVFKDAYPGGGATIGSAITRAYAVGKH
ECCCCCCCCCCCCEECCCCCCCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
ISQSFQMETTEALSLTH
HHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]