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The map label for this gene is poxB [H]

Identifier: 145296606

GI number: 145296606

Start: 2775812

End: 2777551

Strand: Reverse

Name: poxB [H]

Synonym: cgR_2514

Alternate gene names: 145296606

Gene position: 2777551-2775812 (Counterclockwise)

Preceding gene: 145296610

Following gene: 145296605

Centisome position: 83.81

GC content: 52.87

Gene sequence:

>1740_bases
ATGGCACGCAGCTACGCAGAACAATTAATTGACACTTTGGAAGCTCAAGGTGTGAAGCGAATTTATGGTTTGGTGGGTGA
CAGTCTCAATCCGATCGTGGATGCTGTCCGCCAATCAGGTATTGAGTGGGTGCACGTTCGAAATGAGGAAGCGGCGGCGT
TTGCAGCTGGTGCGGAATCGTTGATCACTGGGGAGCTGGCAGTATGTGCTGCTTCTTGTGGTCCTGGAAACACACACCTG
ATTCAGGGGCTTTATGATTCGCATCGAAATGGTGCGAAGGTGTTGGCCATCGCTAGCCATATTCCGAGTGCCCAGATTGG
TTCGACGTTCTTCCAGGAAACGCATCCGGAGATTTTGTTTAAGGAATGCTCTGGTTACTGCGAGATGGTGAATGGTGGTG
AGCAGGGTGAACGCATTTTGCATCACGCGATTCAGTCCACCATGGCGGGTAAAGGTGTGTCGGTGGTAGTGATTCCTGGT
GATATCGCTAAGGAAGACGCAGGTGACGGCACTTATTCCAATTCCACTATTTCCTCGGGTACTCCTGTGGTGTTCCCGGA
TCCTACTGAGGCTGCAGCGTTAGTGGAGGCGATTAACAACGCTAAGACTGTCACGCTGTTCTGCGGTGCGGGCGTGAAGA
ATGCTCGCGCGCAGGTGTTGGAGTTGGCGGAGAAGATTAAGTCACCGATCGGACATGCGTTGGGTGGTAAGCAGTACATT
CAGCATGAGAATCCTTTTGAGGTTGGCATGTCTGGTCTGCTTGGTTACGGTGCCTGCGTGGATGCGTCCAATGAGGCAGA
CCTGCTGATTCTGCTGGGCACGGATTTTCCTTATTCCGATTTCCTTCCCAAAGACAACGTCGCTCAGGTGGATATCAACG
GCGCACACATTGGTCGACGCACCACGGTGAAATACCCGGTGACCGGTGATGTTGCTGCAACGATCGAGAATATTTTGCCT
CATGTGAAGGAAAAAACGGATCGTTCCTTCCTGGATCGCATGCTCAAGGCACACGAGCGCAAGTTGAGCTCGGTGGTGGA
GACGTACACACATAACGTCGAGAAGCATGTGCCTATTCACCCTGAATACGTTGCCTCTATTTTGAATGAGCTGGCGGATA
AGGACGCGGTGTTTACCGTGGACACCGGAATGTGCAATGTGTGGCATGCGAGGTACATCGAAAATCCGGAAGGAACGCGC
GACTTTGTGGGTTCCTTCCGCCACGGCACGATGGCTAATGCGTTGCCTCATGCGATTGGTGCGCAAAGTGTCGATCGGAA
CCGCCAGGTGATCGCGATGTGTGGCGATGGTGGTTTGGGCATGCTGCTGGGTGAGCTTCTGACCGTTAAGCTGCACCAAC
TTCCGCTGAAGGCTGTGGTGTTTAACAACAGTTCTTTGGGCATGGTGAAGTTGGAGATGCTCGTGGAGGGGCAGCCGGAA
TTTGGCACGGATCATGAGGAAGTGAATTTCGCAGAGATTGCGGCGGCTGCGGGTATCAAGTCGGTACGCATCACCGATCC
GAAGAAAGTTCGCGAGCAGCTAGCTGAGGCATTGGCATATCCTGGACCTGTACTGATCGATATCGTTACGGATCCTAATG
CGCTGTCGATCCCACCAACTATCACGTGGGAACAAATCATGGGATTCAGCAAGGCAGCCACCCGAACCGTCTTTGGTGGA
GGAGTGGGAGCGATGATCGATCTCGCCCGTTCGAACATAAGGAATATTCCTACTCCATGA

Upstream 100 bases:

>100_bases
CTGGCAGGCGGGCGAAGCGTGGCAACAACTCGAATTGAAGAGCACAATTGAAGTCGCACCAAGTTAGGCAACACAATAGC
CATAACGTTGAGGAGTTCAG

Downstream 100 bases:

>100_bases
TGATTGATACACCTGCTGTTCTCATTGACCGCGAGCGCTTGACCGCCAACATTTTCAAAATGGCGGCCCACGCCGCAGCC
AATGGGATTGCTCTGCGTCC

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MARSYAEQLIDTLEAQGVKRIYGLVGDSLNPIVDAVRQSGIEWVHVRNEEAAAFAAGAESLITGELAVCAASCGPGNTHL
IQGLYDSHRNGAKVLAIASHIPSAQIGSTFFQETHPEILFKECSGYCEMVNGGEQGERILHHAIQSTMAGKGVSVVVIPG
DIAKEDAGDGTYSNSTISSGTPVVFPDPTEAAALVEAINNAKTVTLFCGAGVKNARAQVLELAEKIKSPIGHALGGKQYI
QHENPFEVGMSGLLGYGACVDASNEADLLILLGTDFPYSDFLPKDNVAQVDINGAHIGRRTTVKYPVTGDVAATIENILP
HVKEKTDRSFLDRMLKAHERKLSSVVETYTHNVEKHVPIHPEYVASILNELADKDAVFTVDTGMCNVWHARYIENPEGTR
DFVGSFRHGTMANALPHAIGAQSVDRNRQVIAMCGDGGLGMLLGELLTVKLHQLPLKAVVFNNSSLGMVKLEMLVEGQPE
FGTDHEEVNFAEIAAAAGIKSVRITDPKKVREQLAEALAYPGPVLIDIVTDPNALSIPPTITWEQIMGFSKAATRTVFGG
GVGAMIDLARSNIRNIPTP

Sequences:

>Translated_579_residues
MARSYAEQLIDTLEAQGVKRIYGLVGDSLNPIVDAVRQSGIEWVHVRNEEAAAFAAGAESLITGELAVCAASCGPGNTHL
IQGLYDSHRNGAKVLAIASHIPSAQIGSTFFQETHPEILFKECSGYCEMVNGGEQGERILHHAIQSTMAGKGVSVVVIPG
DIAKEDAGDGTYSNSTISSGTPVVFPDPTEAAALVEAINNAKTVTLFCGAGVKNARAQVLELAEKIKSPIGHALGGKQYI
QHENPFEVGMSGLLGYGACVDASNEADLLILLGTDFPYSDFLPKDNVAQVDINGAHIGRRTTVKYPVTGDVAATIENILP
HVKEKTDRSFLDRMLKAHERKLSSVVETYTHNVEKHVPIHPEYVASILNELADKDAVFTVDTGMCNVWHARYIENPEGTR
DFVGSFRHGTMANALPHAIGAQSVDRNRQVIAMCGDGGLGMLLGELLTVKLHQLPLKAVVFNNSSLGMVKLEMLVEGQPE
FGTDHEEVNFAEIAAAAGIKSVRITDPKKVREQLAEALAYPGPVLIDIVTDPNALSIPPTITWEQIMGFSKAATRTVFGG
GVGAMIDLARSNIRNIPTP
>Mature_578_residues
ARSYAEQLIDTLEAQGVKRIYGLVGDSLNPIVDAVRQSGIEWVHVRNEEAAAFAAGAESLITGELAVCAASCGPGNTHLI
QGLYDSHRNGAKVLAIASHIPSAQIGSTFFQETHPEILFKECSGYCEMVNGGEQGERILHHAIQSTMAGKGVSVVVIPGD
IAKEDAGDGTYSNSTISSGTPVVFPDPTEAAALVEAINNAKTVTLFCGAGVKNARAQVLELAEKIKSPIGHALGGKQYIQ
HENPFEVGMSGLLGYGACVDASNEADLLILLGTDFPYSDFLPKDNVAQVDINGAHIGRRTTVKYPVTGDVAATIENILPH
VKEKTDRSFLDRMLKAHERKLSSVVETYTHNVEKHVPIHPEYVASILNELADKDAVFTVDTGMCNVWHARYIENPEGTRD
FVGSFRHGTMANALPHAIGAQSVDRNRQVIAMCGDGGLGMLLGELLTVKLHQLPLKAVVFNNSSLGMVKLEMLVEGQPEF
GTDHEEVNFAEIAAAAGIKSVRITDPKKVREQLAEALAYPGPVLIDIVTDPNALSIPPTITWEQIMGFSKAATRTVFGGG
VGAMIDLARSNIRNIPTP

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=569, Percent_Identity=23.725834797891, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1787096, Length=574, Percent_Identity=46.3414634146341, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1790104, Length=540, Percent_Identity=28.5185185185185, Blast_Score=190, Evalue=3e-49,
Organism=Escherichia coli, GI87081685, Length=536, Percent_Identity=26.6791044776119, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1786717, Length=561, Percent_Identity=23.3511586452763, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788716, Length=551, Percent_Identity=20.508166969147, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17531299, Length=475, Percent_Identity=23.5789473684211, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17531301, Length=475, Percent_Identity=23.5789473684211, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323755, Length=564, Percent_Identity=22.6950354609929, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320816, Length=487, Percent_Identity=23.2032854209446, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922626, Length=581, Percent_Identity=23.0636833046472, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 61940; Mature: 61809

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSYAEQLIDTLEAQGVKRIYGLVGDSLNPIVDAVRQSGIEWVHVRNEEAAAFAAGAES
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHCCHH
LITGELAVCAASCGPGNTHLIQGLYDSHRNGAKVLAIASHIPSAQIGSTFFQETHPEILF
HHHHHHHHEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHH
KECSGYCEMVNGGEQGERILHHAIQSTMAGKGVSVVVIPGDIAKEDAGDGTYSNSTISSG
HHHCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
TPVVFPDPTEAAALVEAINNAKTVTLFCGAGVKNARAQVLELAEKIKSPIGHALGGKQYI
CCEECCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
QHENPFEVGMSGLLGYGACVDASNEADLLILLGTDFPYSDFLPKDNVAQVDINGAHIGRR
CCCCCHHHHHHHHHHCCEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCE
TTVKYPVTGDVAATIENILPHVKEKTDRSFLDRMLKAHERKLSSVVETYTHNVEKHVPIH
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PEYVASILNELADKDAVFTVDTGMCNVWHARYIENPEGTRDFVGSFRHGTMANALPHAIG
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHC
AQSVDRNRQVIAMCGDGGLGMLLGELLTVKLHQLPLKAVVFNNSSLGMVKLEMLVEGQPE
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEECCCCC
FGTDHEEVNFAEIAAAAGIKSVRITDPKKVREQLAEALAYPGPVLIDIVTDPNALSIPPT
CCCCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
ITWEQIMGFSKAATRTVFGGGVGAMIDLARSNIRNIPTP
CCHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARSYAEQLIDTLEAQGVKRIYGLVGDSLNPIVDAVRQSGIEWVHVRNEEAAAFAAGAES
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHCCHH
LITGELAVCAASCGPGNTHLIQGLYDSHRNGAKVLAIASHIPSAQIGSTFFQETHPEILF
HHHHHHHHEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHH
KECSGYCEMVNGGEQGERILHHAIQSTMAGKGVSVVVIPGDIAKEDAGDGTYSNSTISSG
HHHCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
TPVVFPDPTEAAALVEAINNAKTVTLFCGAGVKNARAQVLELAEKIKSPIGHALGGKQYI
CCEECCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
QHENPFEVGMSGLLGYGACVDASNEADLLILLGTDFPYSDFLPKDNVAQVDINGAHIGRR
CCCCCHHHHHHHHHHCCEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCE
TTVKYPVTGDVAATIENILPHVKEKTDRSFLDRMLKAHERKLSSVVETYTHNVEKHVPIH
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PEYVASILNELADKDAVFTVDTGMCNVWHARYIENPEGTRDFVGSFRHGTMANALPHAIG
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHC
AQSVDRNRQVIAMCGDGGLGMLLGELLTVKLHQLPLKAVVFNNSSLGMVKLEMLVEGQPE
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEECCCCC
FGTDHEEVNFAEIAAAAGIKSVRITDPKKVREQLAEALAYPGPVLIDIVTDPNALSIPPT
CCCCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
ITWEQIMGFSKAATRTVFGGGVGAMIDLARSNIRNIPTP
CCHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]