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The map label for this gene is purD [H]

Identifier: 145296596

GI number: 145296596

Start: 2765203

End: 2766468

Strand: Reverse

Name: purD [H]

Synonym: cgR_2504

Alternate gene names: 145296596

Gene position: 2766468-2765203 (Counterclockwise)

Preceding gene: 145296598

Following gene: 145296595

Centisome position: 83.47

GC content: 56.24

Gene sequence:

>1266_bases
ATGCGCATTCTGGTAATCGGCTCGGGCGCCCGTGAGCACGCCCTCCTCCGTGGACTGTCAACTGACCCTGCAACCACTGA
ACTCCACGTTGCCCCAGGTAACGCTGGTCTTGGATCGATCGCAACTGTCCACCCTGGCATCAAGGCTGATGATCCAGAGG
CTGTCACTGCGTTGGCTAAAGAACTGAACTCTGATCTGGTTGTTATCGGCCCAGAGATCCCTTTGGTTGCGGGTGTTGCT
GATGCACTTCGCGCGGCGGGTATCGCTGTGTTTGGCCCTAACAAGGACGCTGCTCGTATCGAGGGCTCCAAGGCTTTCGC
TAAGGATGTCATGGCTGCGCAGGGTGTTCGTACTGCTCATGCTGAGGCTATTACTCCTGGTGCGTCTTCTGAAGATATTG
ATGCTGCCATCGATCGTTTCGGCCCAACCTGGGTTGTGAAGGATGATGGTTTGGCTGCGGGCAAGGGTGTTGTGGTTACC
CCCGATCGTGCAGCAGCACGTGCTCACGTAGATGCAGTGCTTGAGGGCGGAAATCCAGTTTTGCTGGAGTCCTTCCTTGA
TGGCCCTGAGGTTTCCCTGTTCTGCCTGGTTGATGGCGAGACGGTAGTTCCTCTGCTGCCAGCGCAGGATCACAAGCGTG
CGTACGACAACGATGAGGGCCCAAATACTGGTGGCATGGGTGCTTATGCGCCGCTTCCTTGGCTGCCTGAAGATGGTGTG
CAGCGCATTGTCGATGAGGTCTGCGTTCCTGTTGCTCGTGAGATGGTGGCACGTGGTTGCGCGTACTCCGGTCTGCTTTA
TGCAGGTATCGCATGGGGTGCAGAAGGCCCTGCGGTAGTTGAGTTCAACTGCCGCTTCGGCGATCCTGAAACCCAGGCTG
TACTGGCACTACTGAAGACTCCTCTGGCAGTACTGCTCAATGCTGTTGCTACTGGAACTTTGGCAGAGCAGCCAGCACTG
GAGTGGGAGGATGCTTACGCCCTGACTGTGGTGTTGGCTTCTTACAATTACCCAGGGGCACCTCGTACTGGTGATGTCAT
CCGCAACGCTGATGCAGATAACGTTCTTCACGCTGGTACTGCACTTAACGCTGAGGGCGAATTGGTTTCTGCGGGCGGTC
GCGTGCTCAACGTGATCGGTGTTGGTGAGACCCTGGAGGCTGCACGTGATAACGCGTACACCACCATCAAGGACATTGAA
CTTGAGGGAAGCCACTACCGCAGCGATATCGCATTGGCTGCATTAGAGGGTCGTATCTCGATCTAA

Upstream 100 bases:

>100_bases
TGATTTTAAGACGCTTTTCGACGCCACTTTCAACCATTTCCGAACCGCCAAGAATACTGGAATAGCTTGGATCAAGTTTT
GCAGGATAAACTGTGCAACC

Downstream 100 bases:

>100_bases
AAGCAGTACGCAGATAGGCTTGTCTCTTATGAAGCCAAGCACTAGAAGCAATGTTCAGCCGTTTCGCGTCATGCAGATGT
TGGACCGAGTCCACCGTCGC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MRILVIGSGAREHALLRGLSTDPATTELHVAPGNAGLGSIATVHPGIKADDPEAVTALAKELNSDLVVIGPEIPLVAGVA
DALRAAGIAVFGPNKDAARIEGSKAFAKDVMAAQGVRTAHAEAITPGASSEDIDAAIDRFGPTWVVKDDGLAAGKGVVVT
PDRAAARAHVDAVLEGGNPVLLESFLDGPEVSLFCLVDGETVVPLLPAQDHKRAYDNDEGPNTGGMGAYAPLPWLPEDGV
QRIVDEVCVPVAREMVARGCAYSGLLYAGIAWGAEGPAVVEFNCRFGDPETQAVLALLKTPLAVLLNAVATGTLAEQPAL
EWEDAYALTVVLASYNYPGAPRTGDVIRNADADNVLHAGTALNAEGELVSAGGRVLNVIGVGETLEAARDNAYTTIKDIE
LEGSHYRSDIALAALEGRISI

Sequences:

>Translated_421_residues
MRILVIGSGAREHALLRGLSTDPATTELHVAPGNAGLGSIATVHPGIKADDPEAVTALAKELNSDLVVIGPEIPLVAGVA
DALRAAGIAVFGPNKDAARIEGSKAFAKDVMAAQGVRTAHAEAITPGASSEDIDAAIDRFGPTWVVKDDGLAAGKGVVVT
PDRAAARAHVDAVLEGGNPVLLESFLDGPEVSLFCLVDGETVVPLLPAQDHKRAYDNDEGPNTGGMGAYAPLPWLPEDGV
QRIVDEVCVPVAREMVARGCAYSGLLYAGIAWGAEGPAVVEFNCRFGDPETQAVLALLKTPLAVLLNAVATGTLAEQPAL
EWEDAYALTVVLASYNYPGAPRTGDVIRNADADNVLHAGTALNAEGELVSAGGRVLNVIGVGETLEAARDNAYTTIKDIE
LEGSHYRSDIALAALEGRISI
>Mature_421_residues
MRILVIGSGAREHALLRGLSTDPATTELHVAPGNAGLGSIATVHPGIKADDPEAVTALAKELNSDLVVIGPEIPLVAGVA
DALRAAGIAVFGPNKDAARIEGSKAFAKDVMAAQGVRTAHAEAITPGASSEDIDAAIDRFGPTWVVKDDGLAAGKGVVVT
PDRAAARAHVDAVLEGGNPVLLESFLDGPEVSLFCLVDGETVVPLLPAQDHKRAYDNDEGPNTGGMGAYAPLPWLPEDGV
QRIVDEVCVPVAREMVARGCAYSGLLYAGIAWGAEGPAVVEFNCRFGDPETQAVLALLKTPLAVLLNAVATGTLAEQPAL
EWEDAYALTVVLASYNYPGAPRTGDVIRNADADNVLHAGTALNAEGELVSAGGRVLNVIGVGETLEAARDNAYTTIKDIE
LEGSHYRSDIALAALEGRISI

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=41.5492957746479, Blast_Score=302, Evalue=4e-82,
Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=41.5492957746479, Blast_Score=301, Evalue=1e-81,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=41.5492957746479, Blast_Score=301, Evalue=1e-81,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=41.5492957746479, Blast_Score=301, Evalue=1e-81,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=42.6573426573427, Blast_Score=310, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=37.0892018779343, Blast_Score=239, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321203, Length=444, Percent_Identity=36.4864864864865, Blast_Score=245, Evalue=1e-65,
Organism=Drosophila melanogaster, GI62471619, Length=434, Percent_Identity=39.8617511520737, Blast_Score=264, Evalue=9e-71,
Organism=Drosophila melanogaster, GI24582400, Length=434, Percent_Identity=39.8617511520737, Blast_Score=263, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 43473; Mature: 43473

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVIGSGAREHALLRGLSTDPATTELHVAPGNAGLGSIATVHPGIKADDPEAVTALAK
CEEEEEECCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHH
ELNSDLVVIGPEIPLVAGVADALRAAGIAVFGPNKDAARIEGSKAFAKDVMAAQGVRTAH
HHCCCEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCCEECCCHHHHHHHHHHCCCCHHH
AEAITPGASSEDIDAAIDRFGPTWVVKDDGLAAGKGVVVTPDRAAARAHVDAVLEGGNPV
HHHCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCEEECCCHHHHHHHHHHEEECCCEE
LLESFLDGPEVSLFCLVDGETVVPLLPAQDHKRAYDNDEGPNTGGMGAYAPLPWLPEDGV
EEHHHCCCCCEEEEEEECCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
QRIVDEVCVPVAREMVARGCAYSGLLYAGIAWGAEGPAVVEFNCRFGDPETQAVLALLKT
HHHHHHHHHHHHHHHHHHCCHHCCHHEEHEEECCCCCEEEEEEECCCCCHHHHHHHHHHH
PLAVLLNAVATGTLAEQPALEWEDAYALTVVLASYNYPGAPRTGDVIRNADADNVLHAGT
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHCCCCCCCEEECCE
ALNAEGELVSAGGRVLNVIGVGETLEAARDNAYTTIKDIELEGSHYRSDIALAALEGRIS
EECCCCCEEECCCEEEEEEECCHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEECCCCC
I
C
>Mature Secondary Structure
MRILVIGSGAREHALLRGLSTDPATTELHVAPGNAGLGSIATVHPGIKADDPEAVTALAK
CEEEEEECCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHH
ELNSDLVVIGPEIPLVAGVADALRAAGIAVFGPNKDAARIEGSKAFAKDVMAAQGVRTAH
HHCCCEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCCEECCCHHHHHHHHHHCCCCHHH
AEAITPGASSEDIDAAIDRFGPTWVVKDDGLAAGKGVVVTPDRAAARAHVDAVLEGGNPV
HHHCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCEEECCCHHHHHHHHHHEEECCCEE
LLESFLDGPEVSLFCLVDGETVVPLLPAQDHKRAYDNDEGPNTGGMGAYAPLPWLPEDGV
EEHHHCCCCCEEEEEEECCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
QRIVDEVCVPVAREMVARGCAYSGLLYAGIAWGAEGPAVVEFNCRFGDPETQAVLALLKT
HHHHHHHHHHHHHHHHHHCCHHCCHHEEHEEECCCCCEEEEEEECCCCCHHHHHHHHHHH
PLAVLLNAVATGTLAEQPALEWEDAYALTVVLASYNYPGAPRTGDVIRNADADNVLHAGT
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHCCCCCCCEEECCE
ALNAEGELVSAGGRVLNVIGVGETLEAARDNAYTTIKDIELEGSHYRSDIALAALEGRIS
EECCCCCEEECCCEEEEEEECCHHHHHHCCCCEEEEEEEEECCCCCCCCEEEEEECCCCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]