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The map label for this gene is cysK [H]

Identifier: 145296557

GI number: 145296557

Start: 2720925

End: 2721860

Strand: Direct

Name: cysK [H]

Synonym: cgR_2465

Alternate gene names: 145296557

Gene position: 2720925-2721860 (Clockwise)

Preceding gene: 145296554

Following gene: 145296558

Centisome position: 82.1

GC content: 57.69

Gene sequence:

>936_bases
ATGGGCAACGTGTACAACAACATCACCGAAACCATCGGTCGCACCCCACTGGTAAAGCTCAACAAGCTCACCGAAGGCCT
GGACGCCACCGTTCTGGTCAAGATTGAGTCATTCAACCCAGCAAACTCCGTGAAAGACCGCATCGGCCTGGCCATCATTG
AAGCAGCAGAGCAGTCCGGTGAACTGAAGCCGGGTGGCACCATCGTTGAAGCAACCTCCGGCAACACCGGTATCGCACTG
GCAATGGTCGGCGCTGCACGAGGCTACAACGTCGTCCTCACCATGCCAGAGACCATGTCCAACGAGCGTCGCGTCCTGCT
CCGCGCTTACGGTGCAGAAATTGTTCTCACCCCAGGTGCAGCTGGCATGCAGGGCGCAAAGGACAAGGCAGACGAGATCG
TCGCGGAGCGCGAAAACGCAATCCTTGCCCGCCAGTTCGAGAACGAGGCAAACCCAAAGGTTCACCGCGAAACCACTGCT
AAAGAAATCCTCGAAGACACCGACGGCAACGTGGACATCTTCGTCGCAGGTTTCGGTACCGGCGGAACCGTAACTGGCGT
TGGCCAGGTTCTGAAGGAAAAGAACTCAAACGTTGAGATCTACGCCGTTGAGCCAGAGGCTTCCCCACTACTTACCACCG
GCAAGGCTGGCCCACACAAGATCCAGGGCATCGGCGCAAACTTCATCCCCGAGGTCCTGGACCGCAAGGTTCTCGATGAC
GTGCTCACCGTCTCCAACGAAGACGCAATCGCTTACTCCCGCAAGCTTGCTGTCGACGAAGGCATCCTCGGCGGCATCTC
CACCGGCGCAAACATCAAGGCTGCGCTGGACTTGGCTGCAAAGCCAGAGAACGCTGGCAAGACCATCGTCACTGTCATCT
GTGACTTCGGTGAGCGCTACGTCTCCACCGTTTTGTACGAAGACATCCGCGACTAA

Upstream 100 bases:

>100_bases
CAGAGGCTGCTTCAAGTAAATGTTTCGTAATTGTTTACAGCGTTTACTCGAGCGGACAACCAACAAAAACAGCACTTCAA
CGATTGGAGCACACCCCACA

Downstream 100 bases:

>100_bases
TTTTTAGCAGCTGTGAACCACTCAAGCTCTGAGCTTGGGTGGTTTTTTTCATGTCTCAAGGTCGTGCCGGGTGCGATTCG
GGTCGGTTTTGAGTGTCTTT

Product: hypothetical protein

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MGNVYNNITETIGRTPLVKLNKLTEGLDATVLVKIESFNPANSVKDRIGLAIIEAAEQSGELKPGGTIVEATSGNTGIAL
AMVGAARGYNVVLTMPETMSNERRVLLRAYGAEIVLTPGAAGMQGAKDKADEIVAERENAILARQFENEANPKVHRETTA
KEILEDTDGNVDIFVAGFGTGGTVTGVGQVLKEKNSNVEIYAVEPEASPLLTTGKAGPHKIQGIGANFIPEVLDRKVLDD
VLTVSNEDAIAYSRKLAVDEGILGGISTGANIKAALDLAAKPENAGKTIVTVICDFGERYVSTVLYEDIRD

Sequences:

>Translated_311_residues
MGNVYNNITETIGRTPLVKLNKLTEGLDATVLVKIESFNPANSVKDRIGLAIIEAAEQSGELKPGGTIVEATSGNTGIAL
AMVGAARGYNVVLTMPETMSNERRVLLRAYGAEIVLTPGAAGMQGAKDKADEIVAERENAILARQFENEANPKVHRETTA
KEILEDTDGNVDIFVAGFGTGGTVTGVGQVLKEKNSNVEIYAVEPEASPLLTTGKAGPHKIQGIGANFIPEVLDRKVLDD
VLTVSNEDAIAYSRKLAVDEGILGGISTGANIKAALDLAAKPENAGKTIVTVICDFGERYVSTVLYEDIRD
>Mature_310_residues
GNVYNNITETIGRTPLVKLNKLTEGLDATVLVKIESFNPANSVKDRIGLAIIEAAEQSGELKPGGTIVEATSGNTGIALA
MVGAARGYNVVLTMPETMSNERRVLLRAYGAEIVLTPGAAGMQGAKDKADEIVAERENAILARQFENEANPKVHRETTAK
EILEDTDGNVDIFVAGFGTGGTVTGVGQVLKEKNSNVEIYAVEPEASPLLTTGKAGPHKIQGIGANFIPEVLDRKVLDDV
LTVSNEDAIAYSRKLAVDEGILGGISTGANIKAALDLAAKPENAGKTIVTVICDFGERYVSTVLYEDIRD

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=313, Percent_Identity=41.85303514377, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI295821200, Length=313, Percent_Identity=41.85303514377, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI4557415, Length=313, Percent_Identity=41.85303514377, Blast_Score=231, Evalue=8e-61,
Organism=Escherichia coli, GI1788754, Length=318, Percent_Identity=52.5157232704402, Blast_Score=283, Evalue=8e-78,
Organism=Escherichia coli, GI2367138, Length=301, Percent_Identity=41.8604651162791, Blast_Score=233, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17535051, Length=303, Percent_Identity=52.4752475247525, Blast_Score=315, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI115535073, Length=306, Percent_Identity=55.2287581699346, Blast_Score=310, Evalue=7e-85,
Organism=Caenorhabditis elegans, GI17562970, Length=301, Percent_Identity=51.4950166112957, Blast_Score=309, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI32566674, Length=293, Percent_Identity=51.5358361774744, Blast_Score=300, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17561720, Length=301, Percent_Identity=49.8338870431894, Blast_Score=268, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI32566672, Length=215, Percent_Identity=50.6976744186047, Blast_Score=221, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17534315, Length=322, Percent_Identity=37.5776397515528, Blast_Score=169, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI25147552, Length=323, Percent_Identity=34.984520123839, Blast_Score=162, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17561716, Length=121, Percent_Identity=48.7603305785124, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71996324, Length=326, Percent_Identity=26.6871165644172, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=37.1428571428571, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6321449, Length=332, Percent_Identity=36.4457831325301, Blast_Score=157, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24643623, Length=309, Percent_Identity=39.8058252427184, Blast_Score=200, Evalue=8e-52,
Organism=Drosophila melanogaster, GI20129101, Length=309, Percent_Identity=39.8058252427184, Blast_Score=200, Evalue=8e-52,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32866; Mature: 32734

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNVYNNITETIGRTPLVKLNKLTEGLDATVLVKIESFNPANSVKDRIGLAIIEAAEQSG
CCCHHHHHHHHHCCCCCEEHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHEEEEHHHCC
ELKPGGTIVEATSGNTGIALAMVGAARGYNVVLTMPETMSNERRVLLRAYGAEIVLTPGA
CCCCCCEEEEECCCCCCEEEEEEECCCCCEEEEECCHHCCCCCEEEEEECCCEEEECCCC
AGMQGAKDKADEIVAERENAILARQFENEANPKVHRETTAKEILEDTDGNVDIFVAGFGT
CCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCC
GGTVTGVGQVLKEKNSNVEIYAVEPEASPLLTTGKAGPHKIQGIGANFIPEVLDRKVLDD
CCCHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCCCEEEECCCHHHHHHHHHHHHHH
VLTVSNEDAIAYSRKLAVDEGILGGISTGANIKAALDLAAKPENAGKTIVTVICDFGERY
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEHHHHHH
VSTVLYEDIRD
HHHHHHHHHCC
>Mature Secondary Structure 
GNVYNNITETIGRTPLVKLNKLTEGLDATVLVKIESFNPANSVKDRIGLAIIEAAEQSG
CCHHHHHHHHHCCCCCEEHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHEEEEHHHCC
ELKPGGTIVEATSGNTGIALAMVGAARGYNVVLTMPETMSNERRVLLRAYGAEIVLTPGA
CCCCCCEEEEECCCCCCEEEEEEECCCCCEEEEECCHHCCCCCEEEEEECCCEEEECCCC
AGMQGAKDKADEIVAERENAILARQFENEANPKVHRETTAKEILEDTDGNVDIFVAGFGT
CCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCC
GGTVTGVGQVLKEKNSNVEIYAVEPEASPLLTTGKAGPHKIQGIGANFIPEVLDRKVLDD
CCCHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCCCEEEECCCHHHHHHHHHHHHHH
VLTVSNEDAIAYSRKLAVDEGILGGISTGANIKAALDLAAKPENAGKTIVTVICDFGERY
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEHHHHHH
VSTVLYEDIRD
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]