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The map label for this gene is nadE

Identifier: 145296532

GI number: 145296532

Start: 2683645

End: 2684478

Strand: Direct

Name: nadE

Synonym: cgR_2441

Alternate gene names: 145296532

Gene position: 2683645-2684478 (Clockwise)

Preceding gene: 145296527

Following gene: 145296538

Centisome position: 80.97

GC content: 54.2

Gene sequence:

>834_bases
ATGACAAACACTCAAACCGAGATCATTAATGAACTAAAGGTGAGCCCGGCAATCGACGTGGCCAAGGAAGTTGAATTCCG
CGTGCAGTTTCTCGTCGATTACCTACGGGCTTCCCACGCAAAAGGCTTTGTTCTTGGCATTTCCGGCGGCCAGGATTCCA
CCCTTGCGGGACGCCTCGCGCAGCTGGCAGTAGAGCGCATTCGTGCGGAAGAAAACAGCACGGATTATGTCTTCTACGCA
GTTCGCCTCCCCTACGCGATCCAGGCAGATGAGGACGATGCGCAAGTTGCATTGGAATTCATCGCACCGGATAAGAGCGT
GACCGTCAACGTTAAAGACGCAACGGACGCCACCGAAGCAACTGTTGCAGCTGCTTTGGAACTTCCTGAGCTGACCGACT
TCAATCGGGGCAATATTAAAGCTCGCCAACGCATGGTTGCCCAGTACGCGATTGCCGGCCAGTACGGTTTGCTGGTTATT
GGCACTGATCACGCGGCTGAAAATGTCACGGGATTCTTTACCAAATTCGGCGATGGCGCAGCCGACCTGCTTCCTTTGGC
AGGTTTGAGCAAGCGTCAAGGAGCTGCCATTCTGGAGCACCTGGGTGCACCTTCAAGCACATGGACAAAAATCCCTACTG
CTGATTTGGAAGAGGATCGTCCAGCGTTGCCAGATGAGGAAGCGCTTGGTGTGTCGTATGCGGATATCGATAATTATCTG
GAAAATAAGCCCGATGTCAGCGAAGGGGCCCAGCAGCGCATTGAGCACCTATGGAAGGTCGGCCAGCACAAGCGCCACCT
CCCTGTTACCCCGCAGGAAGATTGGTGGCGTTAA

Upstream 100 bases:

>100_bases
TAACCGACTCCAGCACTAAACTCCAAACCCTTGGCCCGCACCGCCAAAGTTTAGCGCGCCCCAAGACACCACCGCGCCAT
GTTTGCCTAGGATTAGGTAC

Downstream 100 bases:

>100_bases
TCCAACAGTTTGAGTGTCGCTTTGGCGGCGTGGAGTAGTTCCACGCCGTAGCTTGGCCCATGAGACGCCGCATGTACGGC
CAAAGGGTGGAGCTGGTGCA

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTNTQTEIINELKVSPAIDVAKEVEFRVQFLVDYLRASHAKGFVLGISGGQDSTLAGRLAQLAVERIRAEENSTDYVFYA
VRLPYAIQADEDDAQVALEFIAPDKSVTVNVKDATDATEATVAAALELPELTDFNRGNIKARQRMVAQYAIAGQYGLLVI
GTDHAAENVTGFFTKFGDGAADLLPLAGLSKRQGAAILEHLGAPSSTWTKIPTADLEEDRPALPDEEALGVSYADIDNYL
ENKPDVSEGAQQRIEHLWKVGQHKRHLPVTPQEDWWR

Sequences:

>Translated_277_residues
MTNTQTEIINELKVSPAIDVAKEVEFRVQFLVDYLRASHAKGFVLGISGGQDSTLAGRLAQLAVERIRAEENSTDYVFYA
VRLPYAIQADEDDAQVALEFIAPDKSVTVNVKDATDATEATVAAALELPELTDFNRGNIKARQRMVAQYAIAGQYGLLVI
GTDHAAENVTGFFTKFGDGAADLLPLAGLSKRQGAAILEHLGAPSSTWTKIPTADLEEDRPALPDEEALGVSYADIDNYL
ENKPDVSEGAQQRIEHLWKVGQHKRHLPVTPQEDWWR
>Mature_276_residues
TNTQTEIINELKVSPAIDVAKEVEFRVQFLVDYLRASHAKGFVLGISGGQDSTLAGRLAQLAVERIRAEENSTDYVFYAV
RLPYAIQADEDDAQVALEFIAPDKSVTVNVKDATDATEATVAAALELPELTDFNRGNIKARQRMVAQYAIAGQYGLLVIG
TDHAAENVTGFFTKFGDGAADLLPLAGLSKRQGAAILEHLGAPSSTWTKIPTADLEEDRPALPDEEALGVSYADIDNYLE
NKPDVSEGAQQRIEHLWKVGQHKRHLPVTPQEDWWR

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=274, Percent_Identity=50.7299270072993, Blast_Score=265, Evalue=3e-72,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_CORGB (A4QGT5)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139353.1
- ProteinModelPortal:   A4QGT5
- SMR:   A4QGT5
- STRING:   A4QGT5
- GeneID:   4991967
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2441
- eggNOG:   COG0171
- HOGENOM:   HBG351567
- OMA:   GLNKRRV
- ProtClustDB:   PRK00768
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30388; Mature: 30257

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: ACT_SITE 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTQTEIINELKVSPAIDVAKEVEFRVQFLVDYLRASHAKGFVLGISGGQDSTLAGRLA
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
QLAVERIRAEENSTDYVFYAVRLPYAIQADEDDAQVALEFIAPDKSVTVNVKDATDATEA
HHHHHHHHHCCCCCCEEEEEEECCEEEECCCCHHHEEEEEECCCCEEEEEECCCCCHHHH
TVAAALELPELTDFNRGNIKARQRMVAQYAIAGQYGLLVIGTDHAAENVTGFFTKFGDGA
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCH
ADLLPLAGLSKRQGAAILEHLGAPSSTWTKIPTADLEEDRPALPDEEALGVSYADIDNYL
HHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHH
ENKPDVSEGAQQRIEHLWKVGQHKRHLPVTPQEDWWR
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCC
>Mature Secondary Structure 
TNTQTEIINELKVSPAIDVAKEVEFRVQFLVDYLRASHAKGFVLGISGGQDSTLAGRLA
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
QLAVERIRAEENSTDYVFYAVRLPYAIQADEDDAQVALEFIAPDKSVTVNVKDATDATEA
HHHHHHHHHCCCCCCEEEEEEECCEEEECCCCHHHEEEEEECCCCEEEEEECCCCCHHHH
TVAAALELPELTDFNRGNIKARQRMVAQYAIAGQYGLLVIGTDHAAENVTGFFTKFGDGA
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCH
ADLLPLAGLSKRQGAAILEHLGAPSSTWTKIPTADLEEDRPALPDEEALGVSYADIDNYL
HHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHH
ENKPDVSEGAQQRIEHLWKVGQHKRHLPVTPQEDWWR
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA