Click here to switch to the map view.

The map label for this gene is nrdE [H]

Identifier: 145296528

GI number: 145296528

Start: 2679574

End: 2681721

Strand: Reverse

Name: nrdE [H]

Synonym: cgR_2437

Alternate gene names: 145296528

Gene position: 2681721-2679574 (Counterclockwise)

Preceding gene: 145296529

Following gene: 145296526

Centisome position: 80.92

GC content: 54.14

Gene sequence:

>2148_bases
TTGACTGCATCGGGAACCACCGTCGTGCCACGCGGAGACCAGATGGACTTCCACGCTCTTAACGCGTTGCTCAACCTTTA
CGATGACAACGGCAAGATCCAGTTTGAGAAAGACCGTGAAGCTGCAAACCAGTACTTCCTGCAGCACGTCAACCAGAACA
CCGTCTTCTTCCACAACCTGCAGGAAAAGATCGACTACCTGGTTGAAAACAAGTACTACGACCCAATCGTTCTGGACAAG
TACGACTTCCAGTTCATCAAGGACCTCTTCAAGCGCGCATACGGATTCAAGTTCCGCTTCCAGTCCTTCCTCGGTGCATA
CAAGTACTACACTTCCTACACCCTGAAGACCTTCGACGGTCGCCGCTACCTCGAGCGTTTCGAAGACCGTGTCTGCATGG
TCGCCCTCACCCTCGCTGACGGCGACCGCCCATTGGCCGAGAACCTGGTCGATGAGATCATGTCTGGCCGTTTCCAACCA
GCAACCCCAACCTTCCTGAACTCCGGCAAGGCACAGCGCGGCGAGCCAGTATCCTGCTTCCTCCTGCGTATCGAAGACAA
CATGGAGTCCATCGGACGTTCCATCAACTCTGCTCTTCAGCTGTCCAAGCGTGGCGGTGGCGTAGCGTTGCTGCTGTCCA
ACCTTCGTGAAGCCGGCGCACCGATTAAGAAGATTGAAAACCAGTCTTCCGGTGTTATCCCAGTGATGAAACTTCTGGAA
GATGCTTTCTCCTACGCTAACCAGCTGGGTGCTCGTCAGGGTGCAGGTGCTGTGTACCTCAACGCTCACCACCCAGATAT
CCTGTCCTTCCTGGATACCAAGCGTGAGAACGCCGATGAGAAGATCCGTATCAAGACCCTGTCCCTGGGTGTTGTGATTC
CGGACATCACCTTCGAGCTCGCTAAGCGCAACGATGACATGTACCTGTTCTCCCCATACGATGTGGAGCGCATTTACGGC
AAGCCTTTCGCAGACATCTCAATCACCGAGCACTACGACGAGATGGTTGATGATGACCGCATCCGCAAGTCCAAGATCAA
CGCGCGTCAGTTCTTCCAGACCCTGGCAGAGATCCAGTTCGAGTCCGGTTACCCATACATCATGTATGAAGACACCGTGA
ATGCATCCAACCCAATCGAAGGCCGCATCACCCACTCAAACCTGTGCTCTGAGATCCTTCAGGTGTCCACCCCATCTGAA
TTCAACGATGACCTGACTTACGCAGAGGTCGGCGAAGACATTTCTTGTAACTTGGGTTCCCTCAACGTTGCAATGGCTAT
GGATTCACCAAACTTTGAGAAGACCATCGAAACCGCAATCCGCGGCTTAACTGCAGTGTCTGAGCAGACCAGCATCGATT
CCGTGCCTTCCATCCGTAAGGGCAACGAAGCAGCTCACGCCATCGGCCTTGGCCAGATGAACCTTCACGGCTACTTCGGT
CGCGAGCACATGCACTACGGCTCCGAGGAAGCCCTGGACTTCACCAACGCATACTTTGCTGCCGTGCTGTACCAGTGCCT
GCGTGCATCCAACAAGATCGCTACTGAGCGTGGAGAGCGTTTCAAGAACTTCGAAAACTCCAAGTATGCAACCGGTGAGT
ACTTCGATGATTTCGATGCAAACGACTTCGCACCAAAGTCCGACAAGGTCAAGGAACTCTTTGCCAAGTCGAACATCCAC
ACCCCAACCGTTGAGGACTGGGCTGCGCTGAAGGCCGACGTGATGGAGCACGGTCTGTTCAACCGCAACCTGCAAGCGGT
TCCACCAACCGGTTCGATCTCCTACATCAACAACTCCACCTCGTCGATCCACCCAATCGCATCCAAGATTGAGATCCGCA
AGGAAGGCAAGATCGGCCGCGTTTACTACCCAGCTCCACACATGGACAATGACAACCTTGAGTACTTCGAGGACGCCTAC
GAAATCGGCTACGAGAAGATCATTGACACCTACGCTGTGGCAACCAAGTACGTTGACCAGGGCCTGTCACTGACCTTGTT
CTTCAAGGACACTGCCACCACCCGTGACATCAACCGTGCGCAGATCTACGCATGGCGCAAGGGCATCAAGACCTTGTACT
ACATTCGCCTGCGCCAGGTTGCTCTGGAAGGCACCGAAGTTGACGGCTGCGTCAGCTGCATGCTGTAA

Upstream 100 bases:

>100_bases
GCTTTGGGGCTGGAACCACAAGAACCAGTTACCTCGCGATAAACCTTAAAACTTAATCAATCAATACAAAGTTCACACCT
CTTTTGAAAGGGACATTCCT

Downstream 100 bases:

>100_bases
AAGCACTTAAAAATATCCCCGACCTGCACAAGGGTTGGGGATATTTTTATGCCCAGAAACTTCCTCCACATTGTGTGACG
TGGTTGTTTGATCACTCAAA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 715; Mature: 714

Protein sequence:

>715_residues
MTASGTTVVPRGDQMDFHALNALLNLYDDNGKIQFEKDREAANQYFLQHVNQNTVFFHNLQEKIDYLVENKYYDPIVLDK
YDFQFIKDLFKRAYGFKFRFQSFLGAYKYYTSYTLKTFDGRRYLERFEDRVCMVALTLADGDRPLAENLVDEIMSGRFQP
ATPTFLNSGKAQRGEPVSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLSNLREAGAPIKKIENQSSGVIPVMKLLE
DAFSYANQLGARQGAGAVYLNAHHPDILSFLDTKRENADEKIRIKTLSLGVVIPDITFELAKRNDDMYLFSPYDVERIYG
KPFADISITEHYDEMVDDDRIRKSKINARQFFQTLAEIQFESGYPYIMYEDTVNASNPIEGRITHSNLCSEILQVSTPSE
FNDDLTYAEVGEDISCNLGSLNVAMAMDSPNFEKTIETAIRGLTAVSEQTSIDSVPSIRKGNEAAHAIGLGQMNLHGYFG
REHMHYGSEEALDFTNAYFAAVLYQCLRASNKIATERGERFKNFENSKYATGEYFDDFDANDFAPKSDKVKELFAKSNIH
TPTVEDWAALKADVMEHGLFNRNLQAVPPTGSISYINNSTSSIHPIASKIEIRKEGKIGRVYYPAPHMDNDNLEYFEDAY
EIGYEKIIDTYAVATKYVDQGLSLTLFFKDTATTRDINRAQIYAWRKGIKTLYYIRLRQVALEGTEVDGCVSCML

Sequences:

>Translated_715_residues
MTASGTTVVPRGDQMDFHALNALLNLYDDNGKIQFEKDREAANQYFLQHVNQNTVFFHNLQEKIDYLVENKYYDPIVLDK
YDFQFIKDLFKRAYGFKFRFQSFLGAYKYYTSYTLKTFDGRRYLERFEDRVCMVALTLADGDRPLAENLVDEIMSGRFQP
ATPTFLNSGKAQRGEPVSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLSNLREAGAPIKKIENQSSGVIPVMKLLE
DAFSYANQLGARQGAGAVYLNAHHPDILSFLDTKRENADEKIRIKTLSLGVVIPDITFELAKRNDDMYLFSPYDVERIYG
KPFADISITEHYDEMVDDDRIRKSKINARQFFQTLAEIQFESGYPYIMYEDTVNASNPIEGRITHSNLCSEILQVSTPSE
FNDDLTYAEVGEDISCNLGSLNVAMAMDSPNFEKTIETAIRGLTAVSEQTSIDSVPSIRKGNEAAHAIGLGQMNLHGYFG
REHMHYGSEEALDFTNAYFAAVLYQCLRASNKIATERGERFKNFENSKYATGEYFDDFDANDFAPKSDKVKELFAKSNIH
TPTVEDWAALKADVMEHGLFNRNLQAVPPTGSISYINNSTSSIHPIASKIEIRKEGKIGRVYYPAPHMDNDNLEYFEDAY
EIGYEKIIDTYAVATKYVDQGLSLTLFFKDTATTRDINRAQIYAWRKGIKTLYYIRLRQVALEGTEVDGCVSCML
>Mature_714_residues
TASGTTVVPRGDQMDFHALNALLNLYDDNGKIQFEKDREAANQYFLQHVNQNTVFFHNLQEKIDYLVENKYYDPIVLDKY
DFQFIKDLFKRAYGFKFRFQSFLGAYKYYTSYTLKTFDGRRYLERFEDRVCMVALTLADGDRPLAENLVDEIMSGRFQPA
TPTFLNSGKAQRGEPVSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLSNLREAGAPIKKIENQSSGVIPVMKLLED
AFSYANQLGARQGAGAVYLNAHHPDILSFLDTKRENADEKIRIKTLSLGVVIPDITFELAKRNDDMYLFSPYDVERIYGK
PFADISITEHYDEMVDDDRIRKSKINARQFFQTLAEIQFESGYPYIMYEDTVNASNPIEGRITHSNLCSEILQVSTPSEF
NDDLTYAEVGEDISCNLGSLNVAMAMDSPNFEKTIETAIRGLTAVSEQTSIDSVPSIRKGNEAAHAIGLGQMNLHGYFGR
EHMHYGSEEALDFTNAYFAAVLYQCLRASNKIATERGERFKNFENSKYATGEYFDDFDANDFAPKSDKVKELFAKSNIHT
PTVEDWAALKADVMEHGLFNRNLQAVPPTGSISYINNSTSSIHPIASKIEIRKEGKIGRVYYPAPHMDNDNLEYFEDAYE
IGYEKIIDTYAVATKYVDQGLSLTLFFKDTATTRDINRAQIYAWRKGIKTLYYIRLRQVALEGTEVDGCVSCML

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=592, Percent_Identity=26.0135135135135, Blast_Score=184, Evalue=3e-46,
Organism=Escherichia coli, GI1789030, Length=703, Percent_Identity=71.6927453769559, Blast_Score=1069, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=579, Percent_Identity=27.1157167530225, Blast_Score=190, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17555242, Length=643, Percent_Identity=26.5940902021773, Blast_Score=186, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6322123, Length=650, Percent_Identity=26.9230769230769, Blast_Score=190, Evalue=8e-49,
Organism=Saccharomyces cerevisiae, GI6320914, Length=661, Percent_Identity=26.4750378214826, Blast_Score=187, Evalue=6e-48,
Organism=Drosophila melanogaster, GI17352481, Length=660, Percent_Identity=23.4848484848485, Blast_Score=165, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 81105; Mature: 80974

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASGTTVVPRGDQMDFHALNALLNLYDDNGKIQFEKDREAANQYFLQHVNQNTVFFHNL
CCCCCCEEECCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEECH
QEKIDYLVENKYYDPIVLDKYDFQFIKDLFKRAYGFKFRFQSFLGAYKYYTSYTLKTFDG
HHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEECCC
RRYLERFEDRVCMVALTLADGDRPLAENLVDEIMSGRFQPATPTFLNSGKAQRGEPVSCF
HHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEEEE
LLRIEDNMESIGRSINSALQLSKRGGGVALLLSNLREAGAPIKKIENQSSGVIPVMKLLE
EEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHH
DAFSYANQLGARQGAGAVYLNAHHPDILSFLDTKRENADEKIRIKTLSLGVVIPDITFEL
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEEEEEEEECCHHHHH
AKRNDDMYLFSPYDVERIYGKPFADISITEHYDEMVDDDRIRKSKINARQFFQTLAEIQF
HHCCCCEEEECCCCHHHHCCCCCCCCCHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHEE
ESGYPYIMYEDTVNASNPIEGRITHSNLCSEILQVSTPSEFNDDLTYAEVGEDISCNLGS
CCCCCEEEEECCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCC
LNVAMAMDSPNFEKTIETAIRGLTAVSEQTSIDSVPSIRKGNEAAHAIGLGQMNLHGYFG
EEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEECCCEEECCCCC
REHMHYGSEEALDFTNAYFAAVLYQCLRASNKIATERGERFKNFENSKYATGEYFDDFDA
CHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCC
NDFAPKSDKVKELFAKSNIHTPTVEDWAALKADVMEHGLFNRNLQAVPPTGSISYINNST
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEECCCC
SSIHPIASKIEIRKEGKIGRVYYPAPHMDNDNLEYFEDAYEIGYEKIIDTYAVATKYVDQ
CCCCHHHHHHEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLSLTLFFKDTATTRDINRAQIYAWRKGIKTLYYIRLRQVALEGTEVDGCVSCML
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC
>Mature Secondary Structure 
TASGTTVVPRGDQMDFHALNALLNLYDDNGKIQFEKDREAANQYFLQHVNQNTVFFHNL
CCCCCEEECCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEECH
QEKIDYLVENKYYDPIVLDKYDFQFIKDLFKRAYGFKFRFQSFLGAYKYYTSYTLKTFDG
HHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEEECCC
RRYLERFEDRVCMVALTLADGDRPLAENLVDEIMSGRFQPATPTFLNSGKAQRGEPVSCF
HHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCEEEE
LLRIEDNMESIGRSINSALQLSKRGGGVALLLSNLREAGAPIKKIENQSSGVIPVMKLLE
EEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHH
DAFSYANQLGARQGAGAVYLNAHHPDILSFLDTKRENADEKIRIKTLSLGVVIPDITFEL
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEEEEEEEECCHHHHH
AKRNDDMYLFSPYDVERIYGKPFADISITEHYDEMVDDDRIRKSKINARQFFQTLAEIQF
HHCCCCEEEECCCCHHHHCCCCCCCCCHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHEE
ESGYPYIMYEDTVNASNPIEGRITHSNLCSEILQVSTPSEFNDDLTYAEVGEDISCNLGS
CCCCCEEEEECCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCC
LNVAMAMDSPNFEKTIETAIRGLTAVSEQTSIDSVPSIRKGNEAAHAIGLGQMNLHGYFG
EEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEECCCEEECCCCC
REHMHYGSEEALDFTNAYFAAVLYQCLRASNKIATERGERFKNFENSKYATGEYFDDFDA
CHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCC
NDFAPKSDKVKELFAKSNIHTPTVEDWAALKADVMEHGLFNRNLQAVPPTGSISYINNST
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEECCCC
SSIHPIASKIEIRKEGKIGRVYYPAPHMDNDNLEYFEDAYEIGYEKIIDTYAVATKYVDQ
CCCCHHHHHHEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLSLTLFFKDTATTRDINRAQIYAWRKGIKTLYYIRLRQVALEGTEVDGCVSCML
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]