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The map label for this gene is rph [H]

Identifier: 145296503

GI number: 145296503

Start: 2656345

End: 2657082

Strand: Reverse

Name: rph [H]

Synonym: cgR_2414

Alternate gene names: 145296503

Gene position: 2657082-2656345 (Counterclockwise)

Preceding gene: 145296504

Following gene: 145296502

Centisome position: 80.17

GC content: 54.47

Gene sequence:

>738_bases
ATGACTTCTTCTTCCAGCTTTTCTCGTTTTGATGGCCGCGCACAGGATCAGATGCGTGCCGTCAAAATCACCCGTGGATT
TACTTCCAACCCTGCAGGCAGCGTGCTTGTAGAATTCGGCAATACTCGTGTCATGTGCACCGCTTCTGTGGAATTGGGTG
TGCCGCGTTTCAAGCGTGATTCAGGTGAAGGCTGGTTGACCGCAGAGTACGCGATGCTTCCTGCTGCGACTGCGGAGCGT
AACCGTCGTGAATCCATGGCCGGCAAGGTCAAGGGACGCACTCATGAAATTTCTCGTCTGATTGGTCGTTCTTTGCGTGC
AGCTGTGGATCTTTCCCAGCTGGGCGAGAACACCATTGCGATTGACTGCGATGTTCTGCAAGCTGACGGCGGTACTCGTA
CTGCATCGATCACCGGTGCGTATGTGGCGCTGGCTGATGCCATCAAGGTTCTGCAGGAGCGCGGCGTTGTTCCAGGCAGC
CCACTTCTTGCACCAGTTGCTGCTGTTTCCGTTGGCCTGGTCGACGGTAATGTATGCCTTGACCTGCCATATGAAGAAGA
TTCCCGCGCCGATGTTGACCTCAACGTTGTTATGACCGAACACGGTGAATTCGTGGAAATTCAGGGTACCGGCGAAGAAA
CTACCTTCACCCGCGCGCAGCTCAACGACATGCTTGACTATGCCGAAAAGGGCTGCCGCGAATTGGTTGCTGCCCAAAAA
GCTGCACTGGGAATCTAA

Upstream 100 bases:

>100_bases
GGCCCCTAGCGCGTGGCGCTGGGGGCCGAGCTGGTGGAGGTGGGCGTCGAAAAGCATCTTGGTGCGCGGGGGTGTTGGGC
TGGAAGGTACCCTGTATTTC

Downstream 100 bases:

>100_bases
GGGAATCTAAAACCACAACAGAGTTAAGGACACCGGATGAAACTTCTTCTTGCATCCAACAACGCAAAGAAACTCAAAGA
ACTCCAGCGGATTTTGGATC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTSSSSFSRFDGRAQDQMRAVKITRGFTSNPAGSVLVEFGNTRVMCTASVELGVPRFKRDSGEGWLTAEYAMLPAATAER
NRRESMAGKVKGRTHEISRLIGRSLRAAVDLSQLGENTIAIDCDVLQADGGTRTASITGAYVALADAIKVLQERGVVPGS
PLLAPVAAVSVGLVDGNVCLDLPYEEDSRADVDLNVVMTEHGEFVEIQGTGEETTFTRAQLNDMLDYAEKGCRELVAAQK
AALGI

Sequences:

>Translated_245_residues
MTSSSSFSRFDGRAQDQMRAVKITRGFTSNPAGSVLVEFGNTRVMCTASVELGVPRFKRDSGEGWLTAEYAMLPAATAER
NRRESMAGKVKGRTHEISRLIGRSLRAAVDLSQLGENTIAIDCDVLQADGGTRTASITGAYVALADAIKVLQERGVVPGS
PLLAPVAAVSVGLVDGNVCLDLPYEEDSRADVDLNVVMTEHGEFVEIQGTGEETTFTRAQLNDMLDYAEKGCRELVAAQK
AALGI
>Mature_244_residues
TSSSSFSRFDGRAQDQMRAVKITRGFTSNPAGSVLVEFGNTRVMCTASVELGVPRFKRDSGEGWLTAEYAMLPAATAERN
RRESMAGKVKGRTHEISRLIGRSLRAAVDLSQLGENTIAIDCDVLQADGGTRTASITGAYVALADAIKVLQERGVVPGSP
LLAPVAAVSVGLVDGNVCLDLPYEEDSRADVDLNVVMTEHGEFVEIQGTGEETTFTRAQLNDMLDYAEKGCRELVAAQKA
ALGI

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=60, Blast_Score=250, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI71981632, Length=180, Percent_Identity=27.2222222222222, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 26159; Mature: 26028

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSSSFSRFDGRAQDQMRAVKITRGFTSNPAGSVLVEFGNTRVMCTASVELGVPRFKRD
CCCCCCCHHCCCCCHHHHEEEEEECCCCCCCCCEEEEEECCEEEEEEEEEECCCCCCCCC
SGEGWLTAEYAMLPAATAERNRRESMAGKVKGRTHEISRLIGRSLRAAVDLSQLGENTIA
CCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
IDCDVLQADGGTRTASITGAYVALADAIKVLQERGVVPGSPLLAPVAAVSVGLVDGNVCL
EEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEEECCCEEE
DLPYEEDSRADVDLNVVMTEHGEFVEIQGTGEETTFTRAQLNDMLDYAEKGCRELVAAQK
ECCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AALGI
HHCCC
>Mature Secondary Structure 
TSSSSFSRFDGRAQDQMRAVKITRGFTSNPAGSVLVEFGNTRVMCTASVELGVPRFKRD
CCCCCCHHCCCCCHHHHEEEEEECCCCCCCCCEEEEEECCEEEEEEEEEECCCCCCCCC
SGEGWLTAEYAMLPAATAERNRRESMAGKVKGRTHEISRLIGRSLRAAVDLSQLGENTIA
CCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
IDCDVLQADGGTRTASITGAYVALADAIKVLQERGVVPGSPLLAPVAAVSVGLVDGNVCL
EEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEEECCCEEE
DLPYEEDSRADVDLNVVMTEHGEFVEIQGTGEETTFTRAQLNDMLDYAEKGCRELVAAQK
ECCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AALGI
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]