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The map label for this gene is lmrB [H]

Identifier: 145296494

GI number: 145296494

Start: 2640403

End: 2641860

Strand: Reverse

Name: lmrB [H]

Synonym: cgR_2405

Alternate gene names: 145296494

Gene position: 2641860-2640403 (Counterclockwise)

Preceding gene: 145296495

Following gene: 145296493

Centisome position: 79.71

GC content: 54.94

Gene sequence:

>1458_bases
TTGTCTTCCTCCAGCAATCCCACCTCCAGCGATTCCACCTCGAGCAATCCCACAACGGAGCAGGTTGGGATCCCGAGAAA
TGTTGTCATCATCTTGGCTGTATTAGTTTTTACAGCCTTTGTCATGATGTTGAATGAGACCACTCTGGCAGTCGCGTTGC
CGTCGATCATGGCGGACTTTGGCATTGAGGCAAATACTGCGCAGTGGTTGCTCACTGGTTTTATGTTGACCATGGCTGTC
GTTCTTCCAGCTACTGGTTGGATGTTGGAACGCTTTACCACTCGTAGTGTGTTTATTTTCGCCACGATGGTCTTCCTGAT
CGGTACTGTGACGGCTGCGTTGTCTCCTACTTTTGCGATTATGCTGGCTGCTCGCGTCGCTCAGGCGATTGGTACCGCTG
TGATTATGCCGCTGCTGATGACTGTCGCGATGACCGTTGTTCCTCCAGAGCGCCGTGGCGCTGTCATGGGTTTGATTGCG
GTCGTGATGGCTGTTGGTCCTGCGCTTGGCCCTAGTGTTGCCGGTTTAGTTCTTAGTTTTTCTTCCTGGCACGCGATTTT
CTGGGTCATGGTTCCGTTGGTGTTTGTGGCAAGCCTGATCGGTACCCTGCGTCTGACCAACGTCAGTGAGCCTAAAAAGA
CTCCTTTGGATGTCATTTCCTTCCTGATTTCCGCAGTGGCTTTCGGTGGCCTTGTCTACGCTTTGAGCTCGATTGGCATC
ATTTTGGAAGGTGATAGAAGCGCGTTGGTCGTGTTGGCTGTCGGCATCATTGCGTTGGTGGTGTTTGTGTGGCGCCAGAT
TGCCATGGGTAAGCAGGATAAGGCGCTGTTGGATCTGCGTCCGTTGGCGATTCGTGAGTACACCATTGCGCTGGTCGTGC
TTCTGACACTGTTCGGTGCGCTGCTCGGTGTCATGAATACGCTGCCGCTTTACCTGCAGGGATCCTTGATGGTCACCGCC
TTGGTCGCGGGTCTAGTGCTGTTGCCAGGTGGTCTTTTGGAAGGTGTGCTGTCGCCATTTGTGGGTCGAATTTATGATCG
TCATGGTCCACGCGGACTCGTGATCGGCGGTATGTCACTCGTTGTGATCTCCCTGTTTGCACTGTCCACCGTCGATGAGT
TCGCCAACGTGTGGTTCATCATCGGCGTACACATCGTGTTCTCCATCGGCCTTGCGCTGCTGTTCACCCCACTGATGACA
GTCGCGCTCGCATCCGTCCCCGACAACATGTACGGCCACGGCTCCGCGATCCTCAACACCCTCCAACAGCTCGCCGGCGC
CGCAGGCACCGCGGTCATGATTGCGGTTTATTCCACCGTCAGCAACAACGCGCTTATCGACGGCGCAACCCAACAAACCG
CCCTCGCCGACGGCGCCAACTCTGCATTCTTCGCCTCAGCGTGCGTGGCAGTGTTTGCATTGATCGTGGGCTTCTTTGTA
AAGAGGCCAGCCCACTAA

Upstream 100 bases:

>100_bases
TAAGCAAGTATCCGCCCCGTTTTCTGCTCGATGCAGGAACTGGGGCGGTATGCATTTACCTCGCGATAGCGCCCTTTGAT
TCGAGAAAGGAGCTGGAGCC

Downstream 100 bases:

>100_bases
GCTAGGTCGCATGATCAGCATTGGAACCGACCTCGTTCACATTTCGGCGTTCGCAGAGCAGCTTGCACAGCCGGGAAGTT
CCTTCATGGAGGTGTTTTCC

Product: hypothetical protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSSSSNPTSSDSTSSNPTTEQVGIPRNVVIILAVLVFTAFVMMLNETTLAVALPSIMADFGIEANTAQWLLTGFMLTMAV
VLPATGWMLERFTTRSVFIFATMVFLIGTVTAALSPTFAIMLAARVAQAIGTAVIMPLLMTVAMTVVPPERRGAVMGLIA
VVMAVGPALGPSVAGLVLSFSSWHAIFWVMVPLVFVASLIGTLRLTNVSEPKKTPLDVISFLISAVAFGGLVYALSSIGI
ILEGDRSALVVLAVGIIALVVFVWRQIAMGKQDKALLDLRPLAIREYTIALVVLLTLFGALLGVMNTLPLYLQGSLMVTA
LVAGLVLLPGGLLEGVLSPFVGRIYDRHGPRGLVIGGMSLVVISLFALSTVDEFANVWFIIGVHIVFSIGLALLFTPLMT
VALASVPDNMYGHGSAILNTLQQLAGAAGTAVMIAVYSTVSNNALIDGATQQTALADGANSAFFASACVAVFALIVGFFV
KRPAH

Sequences:

>Translated_485_residues
MSSSSNPTSSDSTSSNPTTEQVGIPRNVVIILAVLVFTAFVMMLNETTLAVALPSIMADFGIEANTAQWLLTGFMLTMAV
VLPATGWMLERFTTRSVFIFATMVFLIGTVTAALSPTFAIMLAARVAQAIGTAVIMPLLMTVAMTVVPPERRGAVMGLIA
VVMAVGPALGPSVAGLVLSFSSWHAIFWVMVPLVFVASLIGTLRLTNVSEPKKTPLDVISFLISAVAFGGLVYALSSIGI
ILEGDRSALVVLAVGIIALVVFVWRQIAMGKQDKALLDLRPLAIREYTIALVVLLTLFGALLGVMNTLPLYLQGSLMVTA
LVAGLVLLPGGLLEGVLSPFVGRIYDRHGPRGLVIGGMSLVVISLFALSTVDEFANVWFIIGVHIVFSIGLALLFTPLMT
VALASVPDNMYGHGSAILNTLQQLAGAAGTAVMIAVYSTVSNNALIDGATQQTALADGANSAFFASACVAVFALIVGFFV
KRPAH
>Mature_484_residues
SSSSNPTSSDSTSSNPTTEQVGIPRNVVIILAVLVFTAFVMMLNETTLAVALPSIMADFGIEANTAQWLLTGFMLTMAVV
LPATGWMLERFTTRSVFIFATMVFLIGTVTAALSPTFAIMLAARVAQAIGTAVIMPLLMTVAMTVVPPERRGAVMGLIAV
VMAVGPALGPSVAGLVLSFSSWHAIFWVMVPLVFVASLIGTLRLTNVSEPKKTPLDVISFLISAVAFGGLVYALSSIGII
LEGDRSALVVLAVGIIALVVFVWRQIAMGKQDKALLDLRPLAIREYTIALVVLLTLFGALLGVMNTLPLYLQGSLMVTAL
VAGLVLLPGGLLEGVLSPFVGRIYDRHGPRGLVIGGMSLVVISLFALSTVDEFANVWFIIGVHIVFSIGLALLFTPLMTV
ALASVPDNMYGHGSAILNTLQQLAGAAGTAVMIAVYSTVSNNALIDGATQQTALADGANSAFFASACVAVFALIVGFFVK
RPAH

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=435, Percent_Identity=24.8275862068966, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1790195, Length=404, Percent_Identity=25.990099009901, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1789042, Length=425, Percent_Identity=25.1764705882353, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87081983, Length=399, Percent_Identity=27.3182957393484, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1788392, Length=448, Percent_Identity=25.4464285714286, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6325455, Length=157, Percent_Identity=28.6624203821656, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50829; Mature: 50698

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSSNPTSSDSTSSNPTTEQVGIPRNVVIILAVLVFTAFVMMLNETTLAVALPSIMADF
CCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GIEANTAQWLLTGFMLTMAVVLPATGWMLERFTTRSVFIFATMVFLIGTVTAALSPTFAI
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLAARVAQAIGTAVIMPLLMTVAMTVVPPERRGAVMGLIAVVMAVGPALGPSVAGLVLSF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
SSWHAIFWVMVPLVFVASLIGTLRLTNVSEPKKTPLDVISFLISAVAFGGLVYALSSIGI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCE
ILEGDRSALVVLAVGIIALVVFVWRQIAMGKQDKALLDLRPLAIREYTIALVVLLTLFGA
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
LLGVMNTLPLYLQGSLMVTALVAGLVLLPGGLLEGVLSPFVGRIYDRHGPRGLVIGGMSL
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCHHH
VVISLFALSTVDEFANVWFIIGVHIVFSIGLALLFTPLMTVALASVPDNMYGHGSAILNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
LQQLAGAAGTAVMIAVYSTVSNNALIDGATQQTALADGANSAFFASACVAVFALIVGFFV
HHHHHCCCCHHHHHHHHHHHCCCCEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
KRPAH
HCCCC
>Mature Secondary Structure 
SSSSNPTSSDSTSSNPTTEQVGIPRNVVIILAVLVFTAFVMMLNETTLAVALPSIMADF
CCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC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HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]