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The map label for this gene is phoB [H]

Identifier: 145296447

GI number: 145296447

Start: 2596521

End: 2597942

Strand: Direct

Name: phoB [H]

Synonym: cgR_2360

Alternate gene names: 145296447

Gene position: 2596521-2597942 (Clockwise)

Preceding gene: 145296441

Following gene: 145296449

Centisome position: 78.35

GC content: 55.77

Gene sequence:

>1422_bases
ATGTCTTTCAAGCGTCGTTCGCTTGCAATCACTTGCGCCATCACCTCCAGCATTGTTCTCGCTGGAATCTCCGGCACCGC
TGCCGCCCAGTCCTCCTACGTTTCTGGATCTTCAGGACTACTGTCCTCCAGCGAGCTCGATCCTGAAACTCCAGCACCTC
TGGATGCTCCAAAGAACATCATCTACATGATCGGTGACGGCATGGGTTACAACCATGTTTCCGCAACCAACCTGTTTGAA
ACCGGCCAGACCATGTACCAGGTCGAAGGCGAGCCAGGTTCCGTCACCCCAGTTGAGGGTGGCACTCCAGTTCAGGCATT
TGAAGGCACTGAGTGGACCCAGCTTGCACAGTCCACCTTCCAGGACGGAAACTCCTACGACCCTGAGCGCGCGTGGGCTG
ATCACAACTATGTCAATGAGAACTTCACCGACTCCGCTGCTGCAGGAACCGCAATGGCAACTGGTGTGAAGACCACCAAC
GGCATGATTGGCGTTAACCCAGCTAACGAGCCTGCGAAGAACACCTCTGAGTACGCAATTGAAAAGGGCAAGGCAGCTGG
CGTTGTTTCCTCTGTTCCTTTCAACCACGCAACTCCTGCGGCTTGGGCAGCTCACAACTCCAACCGCAATGATCTGCACG
CGATGGCCGAAGAGATGATCAACAGTGATCTCAACGTCATCATGGGTGCTGGCCACCCGTTCTTCGACAACAACGGCAAC
CCAATCACAGAAGCCGACGAAGACTACATGCAGGCTTCCCAGTACGAGCGCCTCGCAAGTGGCGAAACCGACTTCACCTT
CGTCGAAGAGGACGCAGACTTCGAGGCTCTCGCAAACGGCCAAGTTGAAAGTGACAAGTACTTCGGTCTCGCGCAGGTCG
AAGACACCCTCCAGCACAACCGCGACGGCGACTCCGTCACTCCTTACGACGTTCCGCTCAACGACGTCGTCGACCTTTCC
ACCATGTCCAAGGCAGCCCTCAACGTTCTTAACCAGGACGAAGACGGCTTCCACATCATGATCGAAGGTGGCGCAATCGA
CTGGGCAGGCCACGCAAACGACATGGCTCGCGACATCGAAGAAGTCCAAGAGTTCAACAAGGCCGTTGAAACTGTCATCG
AGTGGGTTGAGACCAACTCCTCTTGGGATGAAACCCTCGTGATCGTCACCGCCGACCACGAAACCGGCTACATGACCGGT
CCTAACGATGATCCAAACTGGTCCGCAATGACCGGCGCTGCAGGCATCGTTCCAAACCACGGCTGGCACTCCGGCAACCA
CACCAACCAGCTCGTCCCAGTGTTCGTTCGTGGCGCAGGTGTCTCCGATATCGTTGCTGCTGCCGATCAAGTTGACCCAA
TTCGCGGCAACTACATCGACAACATTGAGATCGCGAACCTGACTTTCAACAAGTGGTGGTAA

Upstream 100 bases:

>100_bases
AACTCTTTGGTTGTCATACCTTTCTTGCTTCATATTCATGAAAAACACATTCCCAGTGAAGCCCTGAAGCACACTCTCGA
GCCCAACATAAGGAATAGAA

Downstream 100 bases:

>100_bases
CCCCCGCTTGACCAGCCGTTTTGGTTAATGCAGCACGGTGAGTTAAAGTTATCCGTGCTGCTTAACGCAGTAATGGTGGC
TGTAGTTCAGCTGGTAGAGC

Product: hypothetical protein

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSFKRRSLAITCAITSSIVLAGISGTAAAQSSYVSGSSGLLSSSELDPETPAPLDAPKNIIYMIGDGMGYNHVSATNLFE
TGQTMYQVEGEPGSVTPVEGGTPVQAFEGTEWTQLAQSTFQDGNSYDPERAWADHNYVNENFTDSAAAGTAMATGVKTTN
GMIGVNPANEPAKNTSEYAIEKGKAAGVVSSVPFNHATPAAWAAHNSNRNDLHAMAEEMINSDLNVIMGAGHPFFDNNGN
PITEADEDYMQASQYERLASGETDFTFVEEDADFEALANGQVESDKYFGLAQVEDTLQHNRDGDSVTPYDVPLNDVVDLS
TMSKAALNVLNQDEDGFHIMIEGGAIDWAGHANDMARDIEEVQEFNKAVETVIEWVETNSSWDETLVIVTADHETGYMTG
PNDDPNWSAMTGAAGIVPNHGWHSGNHTNQLVPVFVRGAGVSDIVAAADQVDPIRGNYIDNIEIANLTFNKWW

Sequences:

>Translated_473_residues
MSFKRRSLAITCAITSSIVLAGISGTAAAQSSYVSGSSGLLSSSELDPETPAPLDAPKNIIYMIGDGMGYNHVSATNLFE
TGQTMYQVEGEPGSVTPVEGGTPVQAFEGTEWTQLAQSTFQDGNSYDPERAWADHNYVNENFTDSAAAGTAMATGVKTTN
GMIGVNPANEPAKNTSEYAIEKGKAAGVVSSVPFNHATPAAWAAHNSNRNDLHAMAEEMINSDLNVIMGAGHPFFDNNGN
PITEADEDYMQASQYERLASGETDFTFVEEDADFEALANGQVESDKYFGLAQVEDTLQHNRDGDSVTPYDVPLNDVVDLS
TMSKAALNVLNQDEDGFHIMIEGGAIDWAGHANDMARDIEEVQEFNKAVETVIEWVETNSSWDETLVIVTADHETGYMTG
PNDDPNWSAMTGAAGIVPNHGWHSGNHTNQLVPVFVRGAGVSDIVAAADQVDPIRGNYIDNIEIANLTFNKWW
>Mature_472_residues
SFKRRSLAITCAITSSIVLAGISGTAAAQSSYVSGSSGLLSSSELDPETPAPLDAPKNIIYMIGDGMGYNHVSATNLFET
GQTMYQVEGEPGSVTPVEGGTPVQAFEGTEWTQLAQSTFQDGNSYDPERAWADHNYVNENFTDSAAAGTAMATGVKTTNG
MIGVNPANEPAKNTSEYAIEKGKAAGVVSSVPFNHATPAAWAAHNSNRNDLHAMAEEMINSDLNVIMGAGHPFFDNNGNP
ITEADEDYMQASQYERLASGETDFTFVEEDADFEALANGQVESDKYFGLAQVEDTLQHNRDGDSVTPYDVPLNDVVDLST
MSKAALNVLNQDEDGFHIMIEGGAIDWAGHANDMARDIEEVQEFNKAVETVIEWVETNSSWDETLVIVTADHETGYMTGP
NDDPNWSAMTGAAGIVPNHGWHSGNHTNQLVPVFVRGAGVSDIVAAADQVDPIRGNYIDNIEIANLTFNKWW

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=379, Percent_Identity=25.5936675461741, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI294660770, Length=374, Percent_Identity=25.1336898395722, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI294660772, Length=298, Percent_Identity=26.1744966442953, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI48994877, Length=437, Percent_Identity=27.6887871853547, Blast_Score=95, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6320689, Length=410, Percent_Identity=26.0975609756098, Blast_Score=93, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19921912, Length=391, Percent_Identity=26.8542199488491, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI21358067, Length=382, Percent_Identity=25.9162303664921, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21355981, Length=367, Percent_Identity=26.9754768392371, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24585213, Length=403, Percent_Identity=26.5508684863524, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355151, Length=396, Percent_Identity=25.5050505050505, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI85725200, Length=412, Percent_Identity=25.2427184466019, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI85815833, Length=412, Percent_Identity=25.2427184466019, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 50837; Mature: 50706

Theoretical pI: Translated: 3.93; Mature: 3.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFKRRSLAITCAITSSIVLAGISGTAAAQSSYVSGSSGLLSSSELDPETPAPLDAPKNI
CCCCCCEEEEEEEEECEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
IYMIGDGMGYNHVSATNLFETGQTMYQVEGEPGSVTPVEGGTPVQAFEGTEWTQLAQSTF
EEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEECCCCHHHHHHHHHH
QDGNSYDPERAWADHNYVNENFTDSAAAGTAMATGVKTTNGMIGVNPANEPAKNTSEYAI
CCCCCCCCHHHHCCCCCCCCCCCCHHHCCCHHHCCCCCCCCEEEECCCCCCCCCCHHHHH
EKGKAAGVVSSVPFNHATPAAWAAHNSNRNDLHAMAEEMINSDLNVIMGAGHPFFDNNGN
HCCCCCCEEECCCCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
PITEADEDYMQASQYERLASGETDFTFVEEDADFEALANGQVESDKYFGLAQVEDTLQHN
CCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCCCCCCCCCEEHHHHHHHHHHC
RDGDSVTPYDVPLNDVVDLSTMSKAALNVLNQDEDGFHIMIEGGAIDWAGHANDMARDIE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCHHHHHHHH
EVQEFNKAVETVIEWVETNSSWDETLVIVTADHETGYMTGPNDDPNWSAMTGAAGIVPNH
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GWHSGNHTNQLVPVFVRGAGVSDIVAAADQVDPIRGNYIDNIEIANLTFNKWW
CCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCC
>Mature Secondary Structure 
SFKRRSLAITCAITSSIVLAGISGTAAAQSSYVSGSSGLLSSSELDPETPAPLDAPKNI
CCCCCEEEEEEEEECEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
IYMIGDGMGYNHVSATNLFETGQTMYQVEGEPGSVTPVEGGTPVQAFEGTEWTQLAQSTF
EEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEECCCCHHHHHHHHHH
QDGNSYDPERAWADHNYVNENFTDSAAAGTAMATGVKTTNGMIGVNPANEPAKNTSEYAI
CCCCCCCCHHHHCCCCCCCCCCCCHHHCCCHHHCCCCCCCCEEEECCCCCCCCCCHHHHH
EKGKAAGVVSSVPFNHATPAAWAAHNSNRNDLHAMAEEMINSDLNVIMGAGHPFFDNNGN
HCCCCCCEEECCCCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
PITEADEDYMQASQYERLASGETDFTFVEEDADFEALANGQVESDKYFGLAQVEDTLQHN
CCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCCCCCCCCCEEHHHHHHHHHHC
RDGDSVTPYDVPLNDVVDLSTMSKAALNVLNQDEDGFHIMIEGGAIDWAGHANDMARDIE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCHHHHHHHH
EVQEFNKAVETVIEWVETNSSWDETLVIVTADHETGYMTGPNDDPNWSAMTGAAGIVPNH
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GWHSGNHTNQLVPVFVRGAGVSDIVAAADQVDPIRGNYIDNIEIANLTFNKWW
CCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]