Click here to switch to the map view.

The map label for this gene is vanB [H]

Identifier: 145296384

GI number: 145296384

Start: 2531423

End: 2532364

Strand: Direct

Name: vanB [H]

Synonym: cgR_2298

Alternate gene names: 145296384

Gene position: 2531423-2532364 (Clockwise)

Preceding gene: 145296383

Following gene: 145296387

Centisome position: 76.38

GC content: 47.88

Gene sequence:

>942_bases
ATGAAAGAAGTTATTGTCACAGATCTCGTCTATGAAACGCCGACGATCATCTCAATCCATCTAGCAAAACTAGACGGCAC
GCCCATTGGGCACTACGTTCCCGGCGCACACATCGATGTCGAAGGTCCAACTGCAGTAACGAGGCAATACTCCTTATGTG
GACGCCCAGATGGTGACGACGCCTATGTCGTTGCAGTAAAGCGAGAGCCAGCCTCCCGTGGTGGTTCTGAAGCTTTGCAC
CAGCTCAGCGTTGGTGACCACCTGAAGATTTCAGAACCTCGAAACCTCATCGGTATCGCAGATCAAGCTAGTCACCATAT
CCTCATCGCCGGTGGTATTGGAATTACACCAATGCACAGCATGGCAAGGTACATGGATGTTAGAGACATCAGCTTTGAGC
TTCATTACTTCGCCTCCAGCGAAGAAGAAGCCGCATTCCTCCCGCTTCTCCGAGAAAAGTGTCCTGAAAAACTACACGCT
CACCTCGGAATCTCTCGAGAAAAGCAATCTGAAGTCCTTAAAGATTTGGTCCGTAATGCTCCAGAAAGCAGCCACGCATA
TCTTTGTGGCCCCGATGGATTCATGAACAAAGTAACCGAGATTCTGTCTGAAAGATTTTCAAATGACGAGATTCATTTTG
AAAACTTCCACGCCGCAGAAATCGATGATTCTCAAAATAGTTCTTTCTCCGTCGAGCTGGACGGAGAAGAATATGAGATC
CCAGCTGATCGCAGCATCGTTGATGTCCTTAACGAAAATGGTGCTGGAATTGATACCTCCTGTGAGGAAGGAATCTGCGG
CACCTGCATCATGTCAGTACTAGAAGGTACCCCTGAGCACCGAGACAATGTGCTCACACCATCTGAACGTGAAGCGAATG
AAACCATGGCAATTTGTGTATCGCGCACAAAAGAACCAAAGCTTGTCCTTGATTATTTCTAG

Upstream 100 bases:

>100_bases
AGCAAGCTTTGTTACCGGCGCATGCTGGGACATCAATGGCGGCCTCTACCTGCGTTAACCCCCCGTACACCATCTGGAAA
GTGAGGAAACAGCAATGTCT

Downstream 100 bases:

>100_bases
CTTGATCAAGCATGGGAACCGGGCTCGCTGATTCAGTGGGCCCGGTTCTTTTTGCCATGATGAGTCCGTGTAGTTGAACC
CGACTCCTAATGAGGAAGTC

Product: hypothetical protein

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKEVIVTDLVYETPTIISIHLAKLDGTPIGHYVPGAHIDVEGPTAVTRQYSLCGRPDGDDAYVVAVKREPASRGGSEALH
QLSVGDHLKISEPRNLIGIADQASHHILIAGGIGITPMHSMARYMDVRDISFELHYFASSEEEAAFLPLLREKCPEKLHA
HLGISREKQSEVLKDLVRNAPESSHAYLCGPDGFMNKVTEILSERFSNDEIHFENFHAAEIDDSQNSSFSVELDGEEYEI
PADRSIVDVLNENGAGIDTSCEEGICGTCIMSVLEGTPEHRDNVLTPSEREANETMAICVSRTKEPKLVLDYF

Sequences:

>Translated_313_residues
MKEVIVTDLVYETPTIISIHLAKLDGTPIGHYVPGAHIDVEGPTAVTRQYSLCGRPDGDDAYVVAVKREPASRGGSEALH
QLSVGDHLKISEPRNLIGIADQASHHILIAGGIGITPMHSMARYMDVRDISFELHYFASSEEEAAFLPLLREKCPEKLHA
HLGISREKQSEVLKDLVRNAPESSHAYLCGPDGFMNKVTEILSERFSNDEIHFENFHAAEIDDSQNSSFSVELDGEEYEI
PADRSIVDVLNENGAGIDTSCEEGICGTCIMSVLEGTPEHRDNVLTPSEREANETMAICVSRTKEPKLVLDYF
>Mature_313_residues
MKEVIVTDLVYETPTIISIHLAKLDGTPIGHYVPGAHIDVEGPTAVTRQYSLCGRPDGDDAYVVAVKREPASRGGSEALH
QLSVGDHLKISEPRNLIGIADQASHHILIAGGIGITPMHSMARYMDVRDISFELHYFASSEEEAAFLPLLREKCPEKLHA
HLGISREKQSEVLKDLVRNAPESSHAYLCGPDGFMNKVTEILSERFSNDEIHFENFHAAEIDDSQNSSFSVELDGEEYEI
PADRSIVDVLNENGAGIDTSCEEGICGTCIMSVLEGTPEHRDNVLTPSEREANETMAICVSRTKEPKLVLDYF

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=320, Percent_Identity=35, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1788903, Length=236, Percent_Identity=28.8135593220339, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 34471; Mature: 34471

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEVIVTDLVYETPTIISIHLAKLDGTPIGHYVPGAHIDVEGPTAVTRQYSLCGRPDGDD
CCCEEEEHHHHCCCCEEEEEEEEECCCCCCCCCCCCEEECCCCHHHHHHHHCCCCCCCCC
AYVVAVKREPASRGGSEALHQLSVGDHLKISEPRNLIGIADQASHHILIAGGIGITPMHS
EEEEEEECCCCCCCHHHHHHHHCCCCCEEECCCCCEEEECCCCCCEEEEECCCCCCHHHH
MARYMDVRDISFELHYFASSEEEAAFLPLLREKCPEKLHAHLGISREKQSEVLKDLVRNA
HHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHCC
PESSHAYLCGPDGFMNKVTEILSERFSNDEIHFENFHAAEIDDSQNSSFSVELDGEEYEI
CCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCCCCEEEEEECCCEEEC
PADRSIVDVLNENGAGIDTSCEEGICGTCIMSVLEGTPEHRDNVLTPSEREANETMAICV
CCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
SRTKEPKLVLDYF
ECCCCCCEEEECC
>Mature Secondary Structure
MKEVIVTDLVYETPTIISIHLAKLDGTPIGHYVPGAHIDVEGPTAVTRQYSLCGRPDGDD
CCCEEEEHHHHCCCCEEEEEEEEECCCCCCCCCCCCEEECCCCHHHHHHHHCCCCCCCCC
AYVVAVKREPASRGGSEALHQLSVGDHLKISEPRNLIGIADQASHHILIAGGIGITPMHS
EEEEEEECCCCCCCHHHHHHHHCCCCCEEECCCCCEEEECCCCCCEEEEECCCCCCHHHH
MARYMDVRDISFELHYFASSEEEAAFLPLLREKCPEKLHAHLGISREKQSEVLKDLVRNA
HHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHCC
PESSHAYLCGPDGFMNKVTEILSERFSNDEIHFENFHAAEIDDSQNSSFSVELDGEEYEI
CCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCCCCEEEEEECCCEEEC
PADRSIVDVLNENGAGIDTSCEEGICGTCIMSVLEGTPEHRDNVLTPSEREANETMAICV
CCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
SRTKEPKLVLDYF
ECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10348863 [H]