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The map label for this gene is benA [H]

Identifier: 145296381

GI number: 145296381

Start: 2528812

End: 2530089

Strand: Direct

Name: benA [H]

Synonym: cgR_2295

Alternate gene names: 145296381

Gene position: 2528812-2530089 (Clockwise)

Preceding gene: 145296380

Following gene: 145296382

Centisome position: 76.3

GC content: 51.33

Gene sequence:

>1278_bases
ATGACCACCACAACTAGTAACCAGACCCGATTCTCTGACCTGGTCGAAGACGACCGTGTCGCTGGCCAGCTGTACACCGA
TCCTGAAATCTTCAAAGAAGAAATGGAGAAGATTTTCTATAAGACCTGGGTATGGGTCGCTCACGAAAGTGAAATCCCAG
AGGCCGGATCATTTAAGACCACATGGGTAGGCGATCAGCCTGTCATTGTAAACCGAGATCGCAAGGGCAACTTCAACACT
CTGCTCAACCGCTGCCGCCACCGTGGAGCAACAGTCTGTGACCAGCGAAAAGGCAAAGCAAACGGATTTACTTGCCCTTA
CCACAACTGGTCCTACACCCACGATGGTCGTCTACGCGGAGTTCCTTACCCTGACGGATACGAAGGTGTGTTGAAAAAGG
AAGAGCTTGGCCTTCAAACTCTCCGAACCGAGAGCTACCTCGGTATGATTTTTGCCACCTTCAATGAAGACATCGAGCCT
CTAGAAGATCACCTAGGTGACGCCAAGATTTGGATGGAACGATTCCTCAAGCAGACCAATGGATACCCTTGCAAGGTCAT
TGGCGCACACAAGTTCCGCTTCAACGGCAACTGGAAGATCCAGCTGGAAAATACAACCGACGGCTACCACTTCCCTATGG
TTCACAAATCATGGATGGCCTCCGTCGACGCAGAAACCGCCGACATGATGTCCTTCATGGATGATCCTGAAGCAGTTACT
CACGCACTTGGAAATGGTCACAGTGTTGGAATTATGGCCACTGCTCATATCGATTTGGATGAAGACGACGGAACTGAAGA
AATCCAGCCACGCTTCAATCACCTCGTTAAAGAACTTGAAGAAGCCGGCGAAAGCCCAGAGCGAATCCGACGCTTCATGC
GATCCATGCACGGTTGTGGCTTCAACCTCAACATGTTCCCCAACATCGCAATGTCTTCTTCATTCTTCCGCGTTCTAATT
CCAATCTCTGTGAACGAGACCGAAATTTGGCACATGGCTATCGGCATGGACGGCGGTCCAGAAAGCATCAACCAGGAAAG
GCTCCGCATTCACGAGCACTTCCAAGGACCATTCGGATTCGGAAGCCCCGACGATGCTGAAGGTTGGGACCGAGTTCAGA
TCGGTGCCGGCGGCAACCCCAAGATGCCCATCCTGATCAACCGCGGACTTGAGCGCGAATACACAACCGAAGAAAACTGG
CCAACCTCCCATGTCACCGATGAAACCGGCATGCGCGAGGCTTACTACAAGTGGAAGAAGCTGATGAGCAATGACTAA

Upstream 100 bases:

>100_bases
ACGCTCTATGGCGCTACTTCAAGATCGAAGCTCGCATGGGCAGAGAACTGACCATCCGTGACGACGTTGTCGTCTGATCA
AAAAACTGAAAGGACTCACC

Downstream 100 bases:

>100_bases
CACCACCCTCCAGACTGAACTGACCAACACCAGCTTCCTAAACGATGAGCGAGTTTTACGCGCCATCCAGCTGATCTGGC
ACGAAGCTGCCCTGCTGGAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTTTTSNQTRFSDLVEDDRVAGQLYTDPEIFKEEMEKIFYKTWVWVAHESEIPEAGSFKTTWVGDQPVIVNRDRKGNFNT
LLNRCRHRGATVCDQRKGKANGFTCPYHNWSYTHDGRLRGVPYPDGYEGVLKKEELGLQTLRTESYLGMIFATFNEDIEP
LEDHLGDAKIWMERFLKQTNGYPCKVIGAHKFRFNGNWKIQLENTTDGYHFPMVHKSWMASVDAETADMMSFMDDPEAVT
HALGNGHSVGIMATAHIDLDEDDGTEEIQPRFNHLVKELEEAGESPERIRRFMRSMHGCGFNLNMFPNIAMSSSFFRVLI
PISVNETEIWHMAIGMDGGPESINQERLRIHEHFQGPFGFGSPDDAEGWDRVQIGAGGNPKMPILINRGLEREYTTEENW
PTSHVTDETGMREAYYKWKKLMSND

Sequences:

>Translated_425_residues
MTTTTSNQTRFSDLVEDDRVAGQLYTDPEIFKEEMEKIFYKTWVWVAHESEIPEAGSFKTTWVGDQPVIVNRDRKGNFNT
LLNRCRHRGATVCDQRKGKANGFTCPYHNWSYTHDGRLRGVPYPDGYEGVLKKEELGLQTLRTESYLGMIFATFNEDIEP
LEDHLGDAKIWMERFLKQTNGYPCKVIGAHKFRFNGNWKIQLENTTDGYHFPMVHKSWMASVDAETADMMSFMDDPEAVT
HALGNGHSVGIMATAHIDLDEDDGTEEIQPRFNHLVKELEEAGESPERIRRFMRSMHGCGFNLNMFPNIAMSSSFFRVLI
PISVNETEIWHMAIGMDGGPESINQERLRIHEHFQGPFGFGSPDDAEGWDRVQIGAGGNPKMPILINRGLEREYTTEENW
PTSHVTDETGMREAYYKWKKLMSND
>Mature_424_residues
TTTTSNQTRFSDLVEDDRVAGQLYTDPEIFKEEMEKIFYKTWVWVAHESEIPEAGSFKTTWVGDQPVIVNRDRKGNFNTL
LNRCRHRGATVCDQRKGKANGFTCPYHNWSYTHDGRLRGVPYPDGYEGVLKKEELGLQTLRTESYLGMIFATFNEDIEPL
EDHLGDAKIWMERFLKQTNGYPCKVIGAHKFRFNGNWKIQLENTTDGYHFPMVHKSWMASVDAETADMMSFMDDPEAVTH
ALGNGHSVGIMATAHIDLDEDDGTEEIQPRFNHLVKELEEAGESPERIRRFMRSMHGCGFNLNMFPNIAMSSSFFRVLIP
ISVNETEIWHMAIGMDGGPESINQERLRIHEHFQGPFGFGSPDDAEGWDRVQIGAGGNPKMPILINRGLEREYTTEENWP
TSHVTDETGMREAYYKWKKLMSND

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB) [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=431, Percent_Identity=29.9303944315545, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788103, Length=208, Percent_Identity=26.4423076923077, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.10 [H]

Molecular weight: Translated: 48768; Mature: 48637

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTTSNQTRFSDLVEDDRVAGQLYTDPEIFKEEMEKIFYKTWVWVAHESEIPEAGSFKT
CCCCCCCCHHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEE
TWVGDQPVIVNRDRKGNFNTLLNRCRHRGATVCDQRKGKANGFTCPYHNWSYTHDGRLRG
EEECCCCEEEECCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCEECCCCCCCCCCCCEEEC
VPYPDGYEGVLKKEELGLQTLRTESYLGMIFATFNEDIEPLEDHLGDAKIWMERFLKQTN
CCCCCCCHHHHHHHHHCHHHHHHHHHHEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHCC
GYPCKVIGAHKFRFNGNWKIQLENTTDGYHFPMVHKSWMASVDAETADMMSFMDDPEAVT
CCCEEEEEEEEEEECCCEEEEEECCCCCEECCHHHHHHHHHCCCHHHHHHHHCCCHHHHH
HALGNGHSVGIMATAHIDLDEDDGTEEIQPRFNHLVKELEEAGESPERIRRFMRSMHGCG
HHHCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
FNLNMFPNIAMSSSFFRVLIPISVNETEIWHMAIGMDGGPESINQERLRIHEHFQGPFGF
CCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCC
GSPDDAEGWDRVQIGAGGNPKMPILINRGLEREYTTEENWPTSHVTDETGMREAYYKWKK
CCCCCCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LMSND
HHCCC
>Mature Secondary Structure 
TTTTSNQTRFSDLVEDDRVAGQLYTDPEIFKEEMEKIFYKTWVWVAHESEIPEAGSFKT
CCCCCCCHHHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEE
TWVGDQPVIVNRDRKGNFNTLLNRCRHRGATVCDQRKGKANGFTCPYHNWSYTHDGRLRG
EEECCCCEEEECCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCEECCCCCCCCCCCCEEEC
VPYPDGYEGVLKKEELGLQTLRTESYLGMIFATFNEDIEPLEDHLGDAKIWMERFLKQTN
CCCCCCCHHHHHHHHHCHHHHHHHHHHEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHCC
GYPCKVIGAHKFRFNGNWKIQLENTTDGYHFPMVHKSWMASVDAETADMMSFMDDPEAVT
CCCEEEEEEEEEEECCCEEEEEECCCCCEECCHHHHHHHHHCCCHHHHHHHHCCCHHHHH
HALGNGHSVGIMATAHIDLDEDDGTEEIQPRFNHLVKELEEAGESPERIRRFMRSMHGCG
HHHCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
FNLNMFPNIAMSSSFFRVLIPISVNETEIWHMAIGMDGGPESINQERLRIHEHFQGPFGF
CCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCC
GSPDDAEGWDRVQIGAGGNPKMPILINRGLEREYTTEENWPTSHVTDETGMREAYYKWKK
CCCCCCCCCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LMSND
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]