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The map label for this gene is benA [H]

Identifier: 145296371

GI number: 145296371

Start: 2515541

End: 2517034

Strand: Direct

Name: benA [H]

Synonym: cgR_2285

Alternate gene names: 145296371

Gene position: 2515541-2517034 (Clockwise)

Preceding gene: 145296361

Following gene: 145296372

Centisome position: 75.9

GC content: 57.76

Gene sequence:

>1494_bases
ATGTCCACACCAGTTTCAAATTTGGCAAGCGTTCAGAAAACTCTGGACCATGCGCTTGAGGACCGCCCTGAAGAGGGAAT
CGTCCGCGTCAACCGCAACATCTTCACCGACCCTGAGATCTTCGAGCTGGAGATGCGCCACATCTTCGAAGGCAACTGGA
TCTACCTGGCTCACGAGTCCCAGATCCCTAACGCCGGCGACTACTTCACCACCTACATTGGCCGTCAGCCGATCATGATC
ACCCGTTCCAAGGACGGCACACTCAACTGCCTAATCAACGCGTGTTCTCACCGTGGCGCCATGCTCTGCCGTCGCAAGAC
TGACAACCGCACCACCTTGACCTGCCCATTCCACGGCTGGACATTCAGCAACGATGGCGCACTGCTCAAGGTCAAGGACG
AAAAAGAAGGCGCCTACCCAGAGAACTTCCGCACCGACGGCTCCCACGATCTGCGTCGCGTTCCTAAGTTCGAGTCCTAC
CGTGGCTTCCTCTTCGGCTCCCTCAACGATGATGTCGTTTCTTTGGAAGAGCACCTCGGCGACACCCGTACCGTCATTGA
CATGCTGGTTGACCAATCCCCAGAAGGCCTCGAAGTACTGCGCGGATCCTCCACCTACACCTACGACGGCAACTGGAAGC
TCCAGACCGAAAACGGTGCAGACGGCTACCACGTTTCCTCCACCCACTGGAACTACGCAGCAACCACCTCCCGCCGTGGC
ACCGGTGAATCCGCTAACGAAACCAAGGCAATGGACGCTGGTACCTGGGGCAAGCAGGGTGGCGGATACTTCTCCTACCC
TTACGGCCACATGCTGCTGTGGATGTGGTGGGGCAACCCAGAAGACCGCCCACTGTTCGAGCGCCGCGACGAGTTCAAGA
AGGAATTCGGCGAAGAAAAGGGCGAGTTCATGGTTGGTGCTTCCCGCAATCTGTGCCTCTACCCCAACGTTTACCTGATG
GATCAGTTCTCCTCACAGATCCGCCACATCCGCCCAATCTCTGTTGATCAGACCGAAGTCACCATCTACTGCATCGCACC
TAAGGGCGAGTCCGCAGAAGCACGTGCGAACCGCATCCGCCAGTACGAGGACTTCTTCAACGCAACCGGCATGGCAACCC
CAGATGACCTGGAGGAATTCCGCTCCTGCCAGAAGACCTACCAGGCATCTGCCTTCCCATGGAATGACATGACCCGCGGT
TTGGGCCACCAGGTACAGGGGCCAAACGAGGTTGCCAAGGGCCTAGGCATGAACGAAGTTCTTTCCTCCGGAGCACGCAC
CGAAGATGAAGGCCTCTACCCAATCCAGCACGGCTTCTGGCATGAACTCATGCAGGACGCTGTGAATAAGCAGAGCATCA
AGGAAAAGGAATTGGCTGACGATACCGCTTCTTCCCTTGCCACCGTAGCTGCAGCCAAAATCCGTGAGGAAGCAAAGGCA
GCGGCGAAGTCCGACGCTGGAGAGCCTCGCCGCCGTCGTCGCACCCGCGGTTAG

Upstream 100 bases:

>100_bases
TGCGGAATTGCTCGCAAATGTCACACACCGCTTCAAAGCAAAAACGAAAACGACATCGCGGTGGCAATACCAACTTCTTT
TCACTCTCTTGGAGGTTCAC

Downstream 100 bases:

>100_bases
TCGTCGAAAAGCAAAAAATCTTTTAAGGAGAACACGTAAATGTCTGAAATCACCCGCTCTGAGATCGAAGCTTTCCTCTA
CTACGAGGCTCGCCTGCTCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSTPVSNLASVQKTLDHALEDRPEEGIVRVNRNIFTDPEIFELEMRHIFEGNWIYLAHESQIPNAGDYFTTYIGRQPIMI
TRSKDGTLNCLINACSHRGAMLCRRKTDNRTTLTCPFHGWTFSNDGALLKVKDEKEGAYPENFRTDGSHDLRRVPKFESY
RGFLFGSLNDDVVSLEEHLGDTRTVIDMLVDQSPEGLEVLRGSSTYTYDGNWKLQTENGADGYHVSSTHWNYAATTSRRG
TGESANETKAMDAGTWGKQGGGYFSYPYGHMLLWMWWGNPEDRPLFERRDEFKKEFGEEKGEFMVGASRNLCLYPNVYLM
DQFSSQIRHIRPISVDQTEVTIYCIAPKGESAEARANRIRQYEDFFNATGMATPDDLEEFRSCQKTYQASAFPWNDMTRG
LGHQVQGPNEVAKGLGMNEVLSSGARTEDEGLYPIQHGFWHELMQDAVNKQSIKEKELADDTASSLATVAAAKIREEAKA
AAKSDAGEPRRRRRTRG

Sequences:

>Translated_497_residues
MSTPVSNLASVQKTLDHALEDRPEEGIVRVNRNIFTDPEIFELEMRHIFEGNWIYLAHESQIPNAGDYFTTYIGRQPIMI
TRSKDGTLNCLINACSHRGAMLCRRKTDNRTTLTCPFHGWTFSNDGALLKVKDEKEGAYPENFRTDGSHDLRRVPKFESY
RGFLFGSLNDDVVSLEEHLGDTRTVIDMLVDQSPEGLEVLRGSSTYTYDGNWKLQTENGADGYHVSSTHWNYAATTSRRG
TGESANETKAMDAGTWGKQGGGYFSYPYGHMLLWMWWGNPEDRPLFERRDEFKKEFGEEKGEFMVGASRNLCLYPNVYLM
DQFSSQIRHIRPISVDQTEVTIYCIAPKGESAEARANRIRQYEDFFNATGMATPDDLEEFRSCQKTYQASAFPWNDMTRG
LGHQVQGPNEVAKGLGMNEVLSSGARTEDEGLYPIQHGFWHELMQDAVNKQSIKEKELADDTASSLATVAAAKIREEAKA
AAKSDAGEPRRRRRTRG
>Mature_496_residues
STPVSNLASVQKTLDHALEDRPEEGIVRVNRNIFTDPEIFELEMRHIFEGNWIYLAHESQIPNAGDYFTTYIGRQPIMIT
RSKDGTLNCLINACSHRGAMLCRRKTDNRTTLTCPFHGWTFSNDGALLKVKDEKEGAYPENFRTDGSHDLRRVPKFESYR
GFLFGSLNDDVVSLEEHLGDTRTVIDMLVDQSPEGLEVLRGSSTYTYDGNWKLQTENGADGYHVSSTHWNYAATTSRRGT
GESANETKAMDAGTWGKQGGGYFSYPYGHMLLWMWWGNPEDRPLFERRDEFKKEFGEEKGEFMVGASRNLCLYPNVYLMD
QFSSQIRHIRPISVDQTEVTIYCIAPKGESAEARANRIRQYEDFFNATGMATPDDLEEFRSCQKTYQASAFPWNDMTRGL
GHQVQGPNEVAKGLGMNEVLSSGARTEDEGLYPIQHGFWHELMQDAVNKQSIKEKELADDTASSLATVAAAKIREEAKAA
AKSDAGEPRRRRRTRG

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB) [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=370, Percent_Identity=30.2702702702703, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1788103, Length=198, Percent_Identity=30.8080808080808, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.10 [H]

Molecular weight: Translated: 56278; Mature: 56147

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPVSNLASVQKTLDHALEDRPEEGIVRVNRNIFTDPEIFELEMRHIFEGNWIYLAHES
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECC
QIPNAGDYFTTYIGRQPIMITRSKDGTLNCLINACSHRGAMLCRRKTDNRTTLTCPFHGW
CCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCC
TFSNDGALLKVKDEKEGAYPENFRTDGSHDLRRVPKFESYRGFLFGSLNDDVVSLEEHLG
EECCCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCHHHHHHHHCC
DTRTVIDMLVDQSPEGLEVLRGSSTYTYDGNWKLQTENGADGYHVSSTHWNYAATTSRRG
HHHHHHHHHHCCCCCCHHHEECCCEEEECCCEEEEECCCCCCEEECCCCCCEEECCCCCC
TGESANETKAMDAGTWGKQGGGYFSYPYGHMLLWMWWGNPEDRPLFERRDEFKKEFGEEK
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCC
GEFMVGASRNLCLYPNVYLMDQFSSQIRHIRPISVDQTEVTIYCIAPKGESAEARANRIR
CCEEEECCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
QYEDFFNATGMATPDDLEEFRSCQKTYQASAFPWNDMTRGLGHQVQGPNEVAKGLGMNEV
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHCCCHHHH
LSSGARTEDEGLYPIQHGFWHELMQDAVNKQSIKEKELADDTASSLATVAAAKIREEAKA
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKSDAGEPRRRRRTRG
HHHCCCCCHHHHHCCCC
>Mature Secondary Structure 
STPVSNLASVQKTLDHALEDRPEEGIVRVNRNIFTDPEIFELEMRHIFEGNWIYLAHES
CCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECC
QIPNAGDYFTTYIGRQPIMITRSKDGTLNCLINACSHRGAMLCRRKTDNRTTLTCPFHGW
CCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCC
TFSNDGALLKVKDEKEGAYPENFRTDGSHDLRRVPKFESYRGFLFGSLNDDVVSLEEHLG
EECCCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCHHHHHHHHCC
DTRTVIDMLVDQSPEGLEVLRGSSTYTYDGNWKLQTENGADGYHVSSTHWNYAATTSRRG
HHHHHHHHHHCCCCCCHHHEECCCEEEECCCEEEEECCCCCCEEECCCCCCEEECCCCCC
TGESANETKAMDAGTWGKQGGGYFSYPYGHMLLWMWWGNPEDRPLFERRDEFKKEFGEEK
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCC
GEFMVGASRNLCLYPNVYLMDQFSSQIRHIRPISVDQTEVTIYCIAPKGESAEARANRIR
CCEEEECCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHH
QYEDFFNATGMATPDDLEEFRSCQKTYQASAFPWNDMTRGLGHQVQGPNEVAKGLGMNEV
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHCCCHHHH
LSSGARTEDEGLYPIQHGFWHELMQDAVNKQSIKEKELADDTASSLATVAAAKIREEAKA
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAKSDAGEPRRRRRTRG
HHHCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]