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The map label for this gene is mdh

Identifier: 145296347

GI number: 145296347

Start: 2492508

End: 2493494

Strand: Reverse

Name: mdh

Synonym: cgR_2262

Alternate gene names: 145296347

Gene position: 2493494-2492508 (Counterclockwise)

Preceding gene: 145296349

Following gene: 145296346

Centisome position: 75.24

GC content: 55.72

Gene sequence:

>987_bases
ATGAATTCCCCGCAGAACGTCTCCACCAAGAAGGTCACCGTCACCGGCGCAGCTGGTCAAATCTCTTATTCACTGTTGTG
GCGCATCGCTAACGGTGAAGTATTCGGCACTGAAACCCCTGTTGAGCTGAAGCTGCTGGAAATCCCTCAGGCACTTGGTG
GGGCAGAGGGCGTGGCCATGGAACTTCTTGATTCAGCCTTCCCACTCCTGCGAAACATCACCATCACCGCCGATGCCAAT
GAGGCATTCGACGGCGCTAATGCGGCGTTTTTGGTCGGTGCGAAGCCTCGCGGAAAAGGCGAAGAGCGCGCAGATCTGCT
GGCTAACAACGGCAAGATTTTCGGACCTCAAGGTAAAGCTATCAATGACAACGCCGCAGATGACATTCGTGTCCTAGTTG
TTGGAAACCCAGCGAACACCAACGCGTTGATTGCTTCAGCTGCGGCCCCAGATGTTCCAGCATCCCGCTTCAACGCAATG
ATGCGCCTTGATCACAACCGTGCGATCTCCCAGCTGGCCACCAAGCTTGGCCGTGGATCTGCGGAATTTAACAACATTGT
GGTCTGGGGAAATCACTCCGCAACCCAGTTCCCAGACATCACCTACGCAACCGTTGGTGGAGAAAAGGTCACCGACCTGG
TTGATCACGATTGGTATGTGGAGGAGTTCATTCCTCGCGTGGCTAACCGTGGCGCTGAAATCATTGAGGTCCGTGGAAAG
TCTTCTGCAGCTTCTGCAGCATCCTCTGCGATTGATCACATGCGCGATTGGGTACAGGGCACCGAGGCGTGGTCCTCTGC
GGCAATTCCTTCCACCGGTGCATACGGCATTCCTGAGGGCATTTTTGTCGGTCTGCCAACCGTATCCCGCAACGGTGAGT
GGGAAATCGTTGAAGGCCTGGAGATTTCCGATTTCCAGCGCGCCCGCATCGACGCGAATGCTCAGGAATTGCAGGCCGAG
CGCGAGGCAGTGCGCGACTTGCTCTAA

Upstream 100 bases:

>100_bases
TGCAGTATCGTCAAGATCACCCAAAACTGGTGGCTGTTCTCTTTTAAGCGGGATAGCATGGGTTCTTAGAGGACCCCCTA
CAAGGATTGAGGATTGTTTA

Downstream 100 bases:

>100_bases
TCTTTAACGCATGACTTCGCTTTTCGACGCCCCAACCCTCCAACGCGTCACCGTTTTCACGGGCTCGGCGCTCGGCAGTT
CCTCGCTGTACACGCAAGCG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MNSPQNVSTKKVTVTGAAGQISYSLLWRIANGEVFGTETPVELKLLEIPQALGGAEGVAMELLDSAFPLLRNITITADAN
EAFDGANAAFLVGAKPRGKGEERADLLANNGKIFGPQGKAINDNAADDIRVLVVGNPANTNALIASAAAPDVPASRFNAM
MRLDHNRAISQLATKLGRGSAEFNNIVVWGNHSATQFPDITYATVGGEKVTDLVDHDWYVEEFIPRVANRGAEIIEVRGK
SSAASAASSAIDHMRDWVQGTEAWSSAAIPSTGAYGIPEGIFVGLPTVSRNGEWEIVEGLEISDFQRARIDANAQELQAE
REAVRDLL

Sequences:

>Translated_328_residues
MNSPQNVSTKKVTVTGAAGQISYSLLWRIANGEVFGTETPVELKLLEIPQALGGAEGVAMELLDSAFPLLRNITITADAN
EAFDGANAAFLVGAKPRGKGEERADLLANNGKIFGPQGKAINDNAADDIRVLVVGNPANTNALIASAAAPDVPASRFNAM
MRLDHNRAISQLATKLGRGSAEFNNIVVWGNHSATQFPDITYATVGGEKVTDLVDHDWYVEEFIPRVANRGAEIIEVRGK
SSAASAASSAIDHMRDWVQGTEAWSSAAIPSTGAYGIPEGIFVGLPTVSRNGEWEIVEGLEISDFQRARIDANAQELQAE
REAVRDLL
>Mature_328_residues
MNSPQNVSTKKVTVTGAAGQISYSLLWRIANGEVFGTETPVELKLLEIPQALGGAEGVAMELLDSAFPLLRNITITADAN
EAFDGANAAFLVGAKPRGKGEERADLLANNGKIFGPQGKAINDNAADDIRVLVVGNPANTNALIASAAAPDVPASRFNAM
MRLDHNRAISQLATKLGRGSAEFNNIVVWGNHSATQFPDITYATVGGEKVTDLVDHDWYVEEFIPRVANRGAEIIEVRGK
SSAASAASSAIDHMRDWVQGTEAWSSAAIPSTGAYGIPEGIFVGLPTVSRNGEWEIVEGLEISDFQRARIDANAQELQAE
REAVRDLL

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=326, Percent_Identity=45.7055214723926, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI89886456, Length=322, Percent_Identity=24.8447204968944, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17561064, Length=320, Percent_Identity=50.9375, Blast_Score=292, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI32566798, Length=261, Percent_Identity=52.8735632183908, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=47.0588235294118, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24583394, Length=325, Percent_Identity=47.3846153846154, Blast_Score=286, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MDH_CORGB (A4QGA0)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139168.1
- ProteinModelPortal:   A4QGA0
- SMR:   A4QGA0
- STRING:   A4QGA0
- GeneID:   4993075
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2262
- eggNOG:   COG0039
- HOGENOM:   HBG289884
- OMA:   RAKCQLA
- ProtClustDB:   PRK05442
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 34922; Mature: 34922

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 192-192 BINDING 97-97 BINDING 103-103 BINDING 110-110 BINDING 117-117 BINDING 136-136 BINDING 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPQNVSTKKVTVTGAAGQISYSLLWRIANGEVFGTETPVELKLLEIPQALGGAEGVAM
CCCCCCCCCEEEEEECCCCCEEEEEEEEEECCEEECCCCCCEEEEEECHHHHCCCCHHHH
ELLDSAFPLLRNITITADANEAFDGANAAFLVGAKPRGKGEERADLLANNGKIFGPQGKA
HHHHHHHHHHHCEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCEEECCCCCC
INDNAADDIRVLVVGNPANTNALIASAAAPDVPASRFNAMMRLDHNRAISQLATKLGRGS
CCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
AEFNNIVVWGNHSATQFPDITYATVGGEKVTDLVDHDWYVEEFIPRVANRGAEIIEVRGK
CCCCCEEEECCCCCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCC
SSAASAASSAIDHMRDWVQGTEAWSSAAIPSTGAYGIPEGIFVGLPTVSRNGEWEIVEGL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEECC
EISDFQRARIDANAQELQAEREAVRDLL
CCCCCHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSPQNVSTKKVTVTGAAGQISYSLLWRIANGEVFGTETPVELKLLEIPQALGGAEGVAM
CCCCCCCCCEEEEEECCCCCEEEEEEEEEECCEEECCCCCCEEEEEECHHHHCCCCHHHH
ELLDSAFPLLRNITITADANEAFDGANAAFLVGAKPRGKGEERADLLANNGKIFGPQGKA
HHHHHHHHHHHCEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCEEECCCCCC
INDNAADDIRVLVVGNPANTNALIASAAAPDVPASRFNAMMRLDHNRAISQLATKLGRGS
CCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
AEFNNIVVWGNHSATQFPDITYATVGGEKVTDLVDHDWYVEEFIPRVANRGAEIIEVRGK
CCCCCEEEECCCCCCCCCCEEEEECCCCHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCC
SSAASAASSAIDHMRDWVQGTEAWSSAAIPSTGAYGIPEGIFVGLPTVSRNGEWEIVEGL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEECC
EISDFQRARIDANAQELQAEREAVRDLL
CCCCCHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA