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The map label for this gene is dkgA [H]

Identifier: 145296327

GI number: 145296327

Start: 2473448

End: 2474305

Strand: Reverse

Name: dkgA [H]

Synonym: cgR_2242

Alternate gene names: 145296327

Gene position: 2474305-2473448 (Counterclockwise)

Preceding gene: 145296331

Following gene: 145296325

Centisome position: 74.66

GC content: 51.52

Gene sequence:

>858_bases
ATGCAATCTTTGATGGATCAGAAGAATAAGCTTTCGAAGTCTGAAGGTATCCCATTGGTCACCTTGAATGATGGAAAAAC
CATTCCTCAGCTTGGTTTTGGTGTGTTCAAGGTAGATCCCGATGAAGCAGAGCGCGTAGTTACCGAAGCACTTGAGGTAG
GTTACCGCCACATCGATACTGCTGCGATTTACGGCAATGAGGAAGGTGTCGGCCGAGCTATTGCTAAGTCCGGCATTCCT
CGTGAAGAGCTGTTTATTACTACCAAGTTGTGGAACGATCGCCACCTGGATGTAGAAGCTGCTTTTGAGGAGTCTCTGCA
GAAGCTGGGCTTGGATTATGTAGATCTGTACTTGGTGCACTGGCCGGCACCGAAGAACGATAATTATGTTGCTGCATGGA
AGGGCTTGGAAAAGCTCGGTGACCGTGCTCGTTCCATCGGTGTGTGTAACTTCCTGCCAGAGCACCTAGAAAAGCTGCTG
GCAGAGGCAACCACTGTGCCTGCCATTAACCAGATTGAGCTGCACCCAGCTTTGCAGCAGCGCGATGCTGTTGAGGCATC
TCTTGCAGCAGGCATCACTGTGGAGTCGTGGGGTCCTCTGGGACAGGGGCGTTTTGATCTTGGCGCTGAGGAACCAATCG
CAGCTGCAGCGAAGAACCACGGAAAGACCCCAGCTCAGGTTGTTATCCGTTGGCACCTGCAGAACGGTTTCGTTGTGTTC
CCCAAGACTGTGACTAAGAGCCGCATGGCGGAAAACATCGACGTGTTTGATTTCGAACTCAGTGATGAGGAGATGGCTGC
GATCACTGCTCTTGAGCGCAATGATCGTGGTGGTTCACACCCGAATGATCTGAACTAG

Upstream 100 bases:

>100_bases
TCAACCTAAGGCGCATTTTGGTGCGCATCAAGGAGAAAATATGTCTGTTGTGGGTACCGGCCAATTCTTTGGATCCCCGG
AGGAAGAGCGGGATAAGTTG

Downstream 100 bases:

>100_bases
AAATAAGGTAAGGCCCTGCACTTCACATAAGTGCAGGGCCTTAATTTGTTTTTGGTGTCTTAGCCGAGGAAGTCAGTCTC
GGTAAACTTGCGATCGAAAG

Product: hypothetical protein

Products: NA

Alternate protein names: 2,5-DKG reductase A; 2,5-DKGR A; 25DKGR-A; AKR5C [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MQSLMDQKNKLSKSEGIPLVTLNDGKTIPQLGFGVFKVDPDEAERVVTEALEVGYRHIDTAAIYGNEEGVGRAIAKSGIP
REELFITTKLWNDRHLDVEAAFEESLQKLGLDYVDLYLVHWPAPKNDNYVAAWKGLEKLGDRARSIGVCNFLPEHLEKLL
AEATTVPAINQIELHPALQQRDAVEASLAAGITVESWGPLGQGRFDLGAEEPIAAAAKNHGKTPAQVVIRWHLQNGFVVF
PKTVTKSRMAENIDVFDFELSDEEMAAITALERNDRGGSHPNDLN

Sequences:

>Translated_285_residues
MQSLMDQKNKLSKSEGIPLVTLNDGKTIPQLGFGVFKVDPDEAERVVTEALEVGYRHIDTAAIYGNEEGVGRAIAKSGIP
REELFITTKLWNDRHLDVEAAFEESLQKLGLDYVDLYLVHWPAPKNDNYVAAWKGLEKLGDRARSIGVCNFLPEHLEKLL
AEATTVPAINQIELHPALQQRDAVEASLAAGITVESWGPLGQGRFDLGAEEPIAAAAKNHGKTPAQVVIRWHLQNGFVVF
PKTVTKSRMAENIDVFDFELSDEEMAAITALERNDRGGSHPNDLN
>Mature_285_residues
MQSLMDQKNKLSKSEGIPLVTLNDGKTIPQLGFGVFKVDPDEAERVVTEALEVGYRHIDTAAIYGNEEGVGRAIAKSGIP
REELFITTKLWNDRHLDVEAAFEESLQKLGLDYVDLYLVHWPAPKNDNYVAAWKGLEKLGDRARSIGVCNFLPEHLEKLL
AEATTVPAINQIELHPALQQRDAVEASLAAGITVESWGPLGQGRFDLGAEEPIAAAAKNHGKTPAQVVIRWHLQNGFVVF
PKTVTKSRMAENIDVFDFELSDEEMAAITALERNDRGGSHPNDLN

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG). 5-keto-D-fructose and dihydroxyacetone can also serve as substrates. 25DKGR-A exhibits a greater selectivity for the substrate and higher thermal stability than

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=293, Percent_Identity=38.5665529010239, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI5174391, Length=293, Percent_Identity=38.5665529010239, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI223468663, Length=298, Percent_Identity=38.9261744966443, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI310109920, Length=298, Percent_Identity=38.255033557047, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI5453543, Length=298, Percent_Identity=38.255033557047, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI45446745, Length=298, Percent_Identity=38.255033557047, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI4503285, Length=298, Percent_Identity=38.255033557047, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI310109922, Length=279, Percent_Identity=39.426523297491, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI24497585, Length=297, Percent_Identity=38.7205387205387, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI24497583, Length=310, Percent_Identity=37.0967741935484, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI93277124, Length=303, Percent_Identity=35.6435643564356, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI291291012, Length=273, Percent_Identity=38.0952380952381, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI4502049, Length=291, Percent_Identity=39.1752577319588, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI5174695, Length=298, Percent_Identity=34.2281879194631, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI300116271, Length=276, Percent_Identity=32.9710144927536, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=32.8519855595668, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI310109926, Length=125, Percent_Identity=45.6, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI207028673, Length=125, Percent_Identity=45.6, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI310109924, Length=154, Percent_Identity=36.3636363636364, Blast_Score=95, Evalue=8e-20,
Organism=Escherichia coli, GI87082198, Length=268, Percent_Identity=50.7462686567164, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1786400, Length=257, Percent_Identity=41.2451361867704, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1788081, Length=268, Percent_Identity=28.3582089552239, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17537077, Length=305, Percent_Identity=35.4098360655738, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17537075, Length=292, Percent_Identity=36.6438356164384, Blast_Score=178, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71998625, Length=269, Percent_Identity=34.5724907063197, Blast_Score=171, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17550248, Length=297, Percent_Identity=34.6801346801347, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17566692, Length=299, Percent_Identity=33.7792642140468, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17564128, Length=299, Percent_Identity=35.7859531772575, Blast_Score=151, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17538386, Length=274, Percent_Identity=35.036496350365, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17561298, Length=270, Percent_Identity=34.0740740740741, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17537079, Length=292, Percent_Identity=30.4794520547945, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17562292, Length=282, Percent_Identity=30.1418439716312, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17552492, Length=263, Percent_Identity=32.319391634981, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17561300, Length=268, Percent_Identity=29.8507462686567, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324694, Length=294, Percent_Identity=39.1156462585034, Blast_Score=215, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6320576, Length=282, Percent_Identity=40.4255319148936, Blast_Score=210, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6322556, Length=257, Percent_Identity=34.6303501945525, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6321896, Length=308, Percent_Identity=31.8181818181818, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319625, Length=302, Percent_Identity=33.112582781457, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6320079, Length=281, Percent_Identity=31.3167259786477, Blast_Score=113, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24644950, Length=300, Percent_Identity=38.6666666666667, Blast_Score=201, Evalue=6e-52,
Organism=Drosophila melanogaster, GI221378297, Length=320, Percent_Identity=36.25, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24657054, Length=287, Percent_Identity=40.418118466899, Blast_Score=192, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24663317, Length=292, Percent_Identity=38.013698630137, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24662789, Length=296, Percent_Identity=34.7972972972973, Blast_Score=180, Evalue=8e-46,
Organism=Drosophila melanogaster, GI20129731, Length=291, Percent_Identity=37.8006872852234, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI21356425, Length=298, Percent_Identity=36.9127516778523, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI45553081, Length=291, Percent_Identity=35.3951890034364, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24662781, Length=295, Percent_Identity=37.6271186440678, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24662785, Length=293, Percent_Identity=38.2252559726962, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI281366140, Length=256, Percent_Identity=34.765625, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 31431; Mature: 31431

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLMDQKNKLSKSEGIPLVTLNDGKTIPQLGFGVFKVDPDEAERVVTEALEVGYRHIDT
CCHHHHHHHHHHHHCCCCEEEECCCCCCHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCE
AAIYGNEEGVGRAIAKSGIPREELFITTKLWNDRHLDVEAAFEESLQKLGLDYVDLYLVH
EEEECCCCCHHHHHHHCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEE
WPAPKNDNYVAAWKGLEKLGDRARSIGVCNFLPEHLEKLLAEATTVPAINQIELHPALQQ
CCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHH
RDAVEASLAAGITVESWGPLGQGRFDLGAEEPIAAAAKNHGKTPAQVVIRWHLQNGFVVF
HHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHEEEEEEECCCEEEE
PKTVTKSRMAENIDVFDFELSDEEMAAITALERNDRGGSHPNDLN
CCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MQSLMDQKNKLSKSEGIPLVTLNDGKTIPQLGFGVFKVDPDEAERVVTEALEVGYRHIDT
CCHHHHHHHHHHHHCCCCEEEECCCCCCHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCE
AAIYGNEEGVGRAIAKSGIPREELFITTKLWNDRHLDVEAAFEESLQKLGLDYVDLYLVH
EEEECCCCCHHHHHHHCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEE
WPAPKNDNYVAAWKGLEKLGDRARSIGVCNFLPEHLEKLLAEATTVPAINQIELHPALQQ
CCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHH
RDAVEASLAAGITVESWGPLGQGRFDLGAEEPIAAAAKNHGKTPAQVVIRWHLQNGFVVF
HHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHEEEEEEECCCEEEE
PKTVTKSRMAENIDVFDFELSDEEMAAITALERNDRGGSHPNDLN
CCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3597405; 10737928; 9618487; 11399090 [H]