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The map label for this gene is gph [C]

Identifier: 145296197

GI number: 145296197

Start: 2336975

End: 2337631

Strand: Direct

Name: gph [C]

Synonym: cgR_2115

Alternate gene names: 145296197

Gene position: 2336975-2337631 (Clockwise)

Preceding gene: 145296193

Following gene: 145296198

Centisome position: 70.51

GC content: 52.66

Gene sequence:

>657_bases
GTGACTACGCCTTCTAAGAAAACTCTGCTCTTTGATCTCGACGGAACCCTCGTCGATTCTTTCCCCGGTATCCGCACTTC
ATTCCTGCACACCCTGCACGAAAAGAACTGGGAAATCCCCTCTGAGGAACGCATCTCGCAAGTTCCAGGACCTCCCATGG
AATGGACGTTCCAGGATTTGGGCATGACTCCAGAGCAGGCACAAGACGCTCTGCAGACCTACCTTGAGCATTACGGCCAG
GTGGGTTGGGATCTTTCCGAAGCATTCCCCGGCATGCGAGATTTGCTGATCCGCTTGAAATACGAAGGTTTCCGCCTGTG
CACCGCCACCTCCAAGGGCGAGTTCTTTGCGGAGAAGGTACTTCGCAAATTCGAGATGTTCGATCTCTTCGAATTCATGG
GTGCCGCCACCGACAACGGCAACCGACGCAGCAAATCTGCCGTGATCAAACATGTCCTCGACAGCGTTGGGTTGGACGAA
CCAAATGATATTTTGATGATTGGTGATCGATCACACGATATTGAAGGTTCGAGTGAATTCGGCATCGATTGTGTTGCCGT
AACCTGGGGCTACGGCAGCAAAACTGAATGGGACGCTGCCCGCTACACCGTGAGCACCGCAGAAGAATTAGAAAGGATCA
TCCATGACTGGGCCTAA

Upstream 100 bases:

>100_bases
CAACGGTTACATCACTCACGCATTAACTCCCCTGGTTGGTACCGCATGTTCGGTTCCCAAGTCTAGCCAAGCCTTGAAAA
ATTCTGGCAAGGTTAATGGT

Downstream 100 bases:

>100_bases
AACTTCGCTACCTGTGGAAATTGTTTTCGTATGCACCGGAAACATTTGCCGATCCCCCATGGCGGAAGTCATCGCGAAGG
CAAAAGCGGAAGAAGCTGGC

Product: hypothetical protein

Products: glycolate; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTTPSKKTLLFDLDGTLVDSFPGIRTSFLHTLHEKNWEIPSEERISQVPGPPMEWTFQDLGMTPEQAQDALQTYLEHYGQ
VGWDLSEAFPGMRDLLIRLKYEGFRLCTATSKGEFFAEKVLRKFEMFDLFEFMGAATDNGNRRSKSAVIKHVLDSVGLDE
PNDILMIGDRSHDIEGSSEFGIDCVAVTWGYGSKTEWDAARYTVSTAEELERIIHDWA

Sequences:

>Translated_218_residues
MTTPSKKTLLFDLDGTLVDSFPGIRTSFLHTLHEKNWEIPSEERISQVPGPPMEWTFQDLGMTPEQAQDALQTYLEHYGQ
VGWDLSEAFPGMRDLLIRLKYEGFRLCTATSKGEFFAEKVLRKFEMFDLFEFMGAATDNGNRRSKSAVIKHVLDSVGLDE
PNDILMIGDRSHDIEGSSEFGIDCVAVTWGYGSKTEWDAARYTVSTAEELERIIHDWA
>Mature_217_residues
TTPSKKTLLFDLDGTLVDSFPGIRTSFLHTLHEKNWEIPSEERISQVPGPPMEWTFQDLGMTPEQAQDALQTYLEHYGQV
GWDLSEAFPGMRDLLIRLKYEGFRLCTATSKGEFFAEKVLRKFEMFDLFEFMGAATDNGNRRSKSAVIKHVLDSVGLDEP
NDILMIGDRSHDIEGSSEFGIDCVAVTWGYGSKTEWDAARYTVSTAEELERIIHDWA

Specific function: Unknown

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=208, Percent_Identity=25.4807692307692, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.18

Molecular weight: Translated: 24767; Mature: 24636

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPSKKTLLFDLDGTLVDSFPGIRTSFLHTLHEKNWEIPSEERISQVPGPPMEWTFQDL
CCCCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHC
GMTPEQAQDALQTYLEHYGQVGWDLSEAFPGMRDLLIRLKYEGFRLCTATSKGEFFAEKV
CCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHH
LRKFEMFDLFEFMGAATDNGNRRSKSAVIKHVLDSVGLDEPNDILMIGDRSHDIEGSSEF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCC
GIDCVAVTWGYGSKTEWDAARYTVSTAEELERIIHDWA
CCEEEEEEECCCCCCCCCHHHEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTPSKKTLLFDLDGTLVDSFPGIRTSFLHTLHEKNWEIPSEERISQVPGPPMEWTFQDL
CCCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHC
GMTPEQAQDALQTYLEHYGQVGWDLSEAFPGMRDLLIRLKYEGFRLCTATSKGEFFAEKV
CCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHH
LRKFEMFDLFEFMGAATDNGNRRSKSAVIKHVLDSVGLDEPNDILMIGDRSHDIEGSSEF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCC
GIDCVAVTWGYGSKTEWDAARYTVSTAEELERIIHDWA
CCEEEEEEECCCCCCCCCHHHEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.57 {phosphoglycolate}} [C]

Substrates: 2-phosphoglycolate; H2O

Specific reaction: 2-phosphoglycolate + H2O = glycolate + phosphate

General reaction: Additional information:

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12368430; 12218036 [H]