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The map label for this gene is metF [H]

Identifier: 145296133

GI number: 145296133

Start: 2262439

End: 2263488

Strand: Reverse

Name: metF [H]

Synonym: cgR_2053

Alternate gene names: 145296133

Gene position: 2263488-2262439 (Counterclockwise)

Preceding gene: 145296136

Following gene: 145296131

Centisome position: 68.3

GC content: 53.14

Gene sequence:

>1050_bases
TTGGTGGGGGTGAATAAATGCCAGAGGCAGTCCCAACAAAACACTTTCATCACACTAAGATACCCAGGCATGTCCCTAAC
GAACATCCCAGCCTCATCTCAATGGGCAATTAGCGACGTTTTGAAGCGTCCTTCACCCGGCCGAGTACCTTTTTCTGTCG
AGTTTATGCCACCCCGCGACGATGCAGCTGAAGAGCGTCTTTACCGCGCAGCAGAGGTCTTCCATGACCTCGGTGCATCG
TTTGTCTCCGTGACTTATGGTGCTGGCGGATCAACCCGTGAGAGAACCTCACGTATTGCTCGACGATTAGCGAAACAACC
GTTGACCACTCTGGTGCACCTGACCCTGGTTAACCACACTCGCGAAGAGATGAAGGCAATTCTTCGGGAATACCTAGAGC
TGGGATTAACAAACCTGTTGGCGCTTCGAGGAGATCCGCCTGGAGACCCATTAGGCGATTGGGTGAGCACCGATGGAGGA
CTGAACTATGCCTCCGAGCTCATCGATCTTATTAAGTCCACTCCTGAGTTCCGGGAATTCGACCTCGGTATCGCCTCCTT
CCCCGAAGGGCATTTCCGGGCGAAAACTCTAGAAGAAGACACCAAATACACTCTGGCGAAGCTGCGTGGAGGTGCAGAGT
ACTCCATCACGCAGATGTTCTTTGATGTGGAAGACTACCTGCGACTTCGTGATCGCCTTGTTGCTGCAGACCCCATTCAC
GGTGCGAAGCCAATCATTCCTGGCATCATGCCCATTACGAGCCTGCGGTCTGTGCGTCGACAGGTCGAACTCTCTGGTGC
TCAATTGCCGAGCCAACTAGAAGAATCACTTGTTCGAGCTGCAAACGGCAATGAAGAAGCGAACAAAGACGAGATCCGCA
AGGTGGGCATTGAATATTCCACCAATATGGCAGAGCGACTCATTGCCGAAGGAGCGGAAGATCTGCACTTCATGACGCTT
AACTTCACCCGTGCAACCCAAGAAGTGTTGTACAACCTTGGCATGGCGCCTGCTTGGGGAGCAGAGCACGGCCAAGACGC
GGTGCGTTAA

Upstream 100 bases:

>100_bases
TCTCGCACGACCTCCGGAAATTTGTCGAGGTCAAGGTCATGGGCATCGAAACTGCTCAAGGAGACGTCCTTCAATCGAAT
AGGGGGATGCGGGCTGAATT

Downstream 100 bases:

>100_bases
GCCCTCTTAGGAATCATGAAGGGGGAGGGCGGTGATCAATACGGCAAACGGCCGTTGATCACCGTCAAACCTAAACTGCC
TGAGCAAGTCAGTGAAGCCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MVGVNKCQRQSQQNTFITLRYPGMSLTNIPASSQWAISDVLKRPSPGRVPFSVEFMPPRDDAAEERLYRAAEVFHDLGAS
FVSVTYGAGGSTRERTSRIARRLAKQPLTTLVHLTLVNHTREEMKAILREYLELGLTNLLALRGDPPGDPLGDWVSTDGG
LNYASELIDLIKSTPEFREFDLGIASFPEGHFRAKTLEEDTKYTLAKLRGGAEYSITQMFFDVEDYLRLRDRLVAADPIH
GAKPIIPGIMPITSLRSVRRQVELSGAQLPSQLEESLVRAANGNEEANKDEIRKVGIEYSTNMAERLIAEGAEDLHFMTL
NFTRATQEVLYNLGMAPAWGAEHGQDAVR

Sequences:

>Translated_349_residues
MVGVNKCQRQSQQNTFITLRYPGMSLTNIPASSQWAISDVLKRPSPGRVPFSVEFMPPRDDAAEERLYRAAEVFHDLGAS
FVSVTYGAGGSTRERTSRIARRLAKQPLTTLVHLTLVNHTREEMKAILREYLELGLTNLLALRGDPPGDPLGDWVSTDGG
LNYASELIDLIKSTPEFREFDLGIASFPEGHFRAKTLEEDTKYTLAKLRGGAEYSITQMFFDVEDYLRLRDRLVAADPIH
GAKPIIPGIMPITSLRSVRRQVELSGAQLPSQLEESLVRAANGNEEANKDEIRKVGIEYSTNMAERLIAEGAEDLHFMTL
NFTRATQEVLYNLGMAPAWGAEHGQDAVR
>Mature_349_residues
MVGVNKCQRQSQQNTFITLRYPGMSLTNIPASSQWAISDVLKRPSPGRVPFSVEFMPPRDDAAEERLYRAAEVFHDLGAS
FVSVTYGAGGSTRERTSRIARRLAKQPLTTLVHLTLVNHTREEMKAILREYLELGLTNLLALRGDPPGDPLGDWVSTDGG
LNYASELIDLIKSTPEFREFDLGIASFPEGHFRAKTLEEDTKYTLAKLRGGAEYSITQMFFDVEDYLRLRDRLVAADPIH
GAKPIIPGIMPITSLRSVRRQVELSGAQLPSQLEESLVRAANGNEEANKDEIRKVGIEYSTNMAERLIAEGAEDLHFMTL
NFTRATQEVLYNLGMAPAWGAEHGQDAVR

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=301, Percent_Identity=35.2159468438538, Blast_Score=161, Evalue=9e-40,
Organism=Escherichia coli, GI1790377, Length=294, Percent_Identity=34.0136054421769, Blast_Score=165, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25147436, Length=296, Percent_Identity=30.4054054054054, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI25147441, Length=296, Percent_Identity=30.4054054054054, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6321313, Length=302, Percent_Identity=30.1324503311258, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=30.6122448979592, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 38879; Mature: 38879

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGVNKCQRQSQQNTFITLRYPGMSLTNIPASSQWAISDVLKRPSPGRVPFSVEFMPPRD
CCCCHHHHHHHCCCCEEEEEECCCEECCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCC
DAAEERLYRAAEVFHDLGASFVSVTYGAGGSTRERTSRIARRLAKQPLTTLVHLTLVNHT
CHHHHHHHHHHHHHHHHCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REEMKAILREYLELGLTNLLALRGDPPGDPLGDWVSTDGGLNYASELIDLIKSTPEFREF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHH
DLGIASFPEGHFRAKTLEEDTKYTLAKLRGGAEYSITQMFFDVEDYLRLRDRLVAADPIH
CCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
GAKPIIPGIMPITSLRSVRRQVELSGAQLPSQLEESLVRAANGNEEANKDEIRKVGIEYS
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHH
TNMAERLIAEGAEDLHFMTLNFTRATQEVLYNLGMAPAWGAEHGQDAVR
HHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVGVNKCQRQSQQNTFITLRYPGMSLTNIPASSQWAISDVLKRPSPGRVPFSVEFMPPRD
CCCCHHHHHHHCCCCEEEEEECCCEECCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCC
DAAEERLYRAAEVFHDLGASFVSVTYGAGGSTRERTSRIARRLAKQPLTTLVHLTLVNHT
CHHHHHHHHHHHHHHHHCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REEMKAILREYLELGLTNLLALRGDPPGDPLGDWVSTDGGLNYASELIDLIKSTPEFREF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHH
DLGIASFPEGHFRAKTLEEDTKYTLAKLRGGAEYSITQMFFDVEDYLRLRDRLVAADPIH
CCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
GAKPIIPGIMPITSLRSVRRQVELSGAQLPSQLEESLVRAANGNEEANKDEIRKVGIEYS
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHH
TNMAERLIAEGAEDLHFMTLNFTRATQEVLYNLGMAPAWGAEHGQDAVR
HHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515933 [H]