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The map label for this gene is mraW

Identifier: 145296128

GI number: 145296128

Start: 2258106

End: 2259119

Strand: Reverse

Name: mraW

Synonym: cgR_2048

Alternate gene names: 145296128

Gene position: 2259119-2258106 (Counterclockwise)

Preceding gene: 145296129

Following gene: 145296127

Centisome position: 68.17

GC content: 54.83

Gene sequence:

>1014_bases
ATGGAAGATTTTTCTTTGGATGGCAACCACGGACACGTTCCCGTAATGCGTGATCGTATGGCGGCTTTGATCGCCGAAAA
CGTGGAAGCATTGGGAGAAAACGCTGTCATTGTTGACGCAACCCTTGGCGCAGGCGGGCATGCGGAGTTCTTCCTGAACA
CGTTCCCCAAAGCGCGCCTGATTGGCCTTGATCGTGACCAAAATGCGTTGCGGGATGCTCGCGCGCGACTTGCTCCTTTC
GGGGAGCGGTTCATCGGCGTCCAGACGCGTTTCGACGGACTCCGCGAGGTGTTGGAATCTGTCGAGGGCGACATCATTGA
TTTAGCTCGCGAGCACGGTATCGCTGGCGCTCTGTTTGATCTGGGTGTCTCCTCGATGCAGCTTGATCAGGTGGAGCGTG
GCTTTGCCTACCGCACGGACGCGCCTCTGGACATGCGCATGGATGCAACTCAGGGTATTACGGCTGCAGATATCCTCAAC
ACTTATTCGCATGGTGACATCGCTCGAATCTTGAAGACTTACGGCGATGAACGCTTCGCCGGCAAGATTGCTTCTGCGGT
GCTGAAAGAACGTGAAAAAGAGCCGTTCACTACCTCTGCTCGTTTGGTGGAGCTTCTGTACGACGCCATCCCTGCAGCGA
CCCGTCGAACCGGTGGACACCCCGCGAAACGTACTTTCCAGGCATTGCGCGTTGAGGTGAACAACGAGCTTGATTCCCTG
AAGAATGTGCTTCCTCAAATCACTGACGCCCTCAATGTTGGGGGACGTGCAGTGTTTATGAGCTACCAGTCTCATGAGGA
CAAGCTGGTGAAGAAGTTCTTCACGGATCTGACCACCTCTAAGACCCCTCCGGGCTTGCCTGTTGATCTTCCTGGAACTG
CACCACAGTTTAAGCAGGTTACTCGCGGTGCTGAAACGGCTTCGGAAGCTGAAATTGAAGAAAACCCACGTGCCGCACCT
GTGAAGGTGCGCGCAATCGAAAGAATCGCCAACAACTCAGGAGACCTCTCATGA

Upstream 100 bases:

>100_bases
TGATATGTGGCTATGACATTGTCGCGCAGTTTTTTTATCCACAAACATAAGTACAAGACATTGGACATCTAGGCCGGCAG
GAAAAGGGGGGAACACGCAC

Downstream 100 bases:

>100_bases
CCATGACAAATGGCTCCCGCACAGAGTCGGGAAAGATTCGCGATCGACGTGTCGCTGACGCTGATCCACGGTATAGCCGT
CGCGTCTCGGTGAGTAGGGA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MEDFSLDGNHGHVPVMRDRMAALIAENVEALGENAVIVDATLGAGGHAEFFLNTFPKARLIGLDRDQNALRDARARLAPF
GERFIGVQTRFDGLREVLESVEGDIIDLAREHGIAGALFDLGVSSMQLDQVERGFAYRTDAPLDMRMDATQGITAADILN
TYSHGDIARILKTYGDERFAGKIASAVLKEREKEPFTTSARLVELLYDAIPAATRRTGGHPAKRTFQALRVEVNNELDSL
KNVLPQITDALNVGGRAVFMSYQSHEDKLVKKFFTDLTTSKTPPGLPVDLPGTAPQFKQVTRGAETASEAEIEENPRAAP
VKVRAIERIANNSGDLS

Sequences:

>Translated_337_residues
MEDFSLDGNHGHVPVMRDRMAALIAENVEALGENAVIVDATLGAGGHAEFFLNTFPKARLIGLDRDQNALRDARARLAPF
GERFIGVQTRFDGLREVLESVEGDIIDLAREHGIAGALFDLGVSSMQLDQVERGFAYRTDAPLDMRMDATQGITAADILN
TYSHGDIARILKTYGDERFAGKIASAVLKEREKEPFTTSARLVELLYDAIPAATRRTGGHPAKRTFQALRVEVNNELDSL
KNVLPQITDALNVGGRAVFMSYQSHEDKLVKKFFTDLTTSKTPPGLPVDLPGTAPQFKQVTRGAETASEAEIEENPRAAP
VKVRAIERIANNSGDLS
>Mature_337_residues
MEDFSLDGNHGHVPVMRDRMAALIAENVEALGENAVIVDATLGAGGHAEFFLNTFPKARLIGLDRDQNALRDARARLAPF
GERFIGVQTRFDGLREVLESVEGDIIDLAREHGIAGALFDLGVSSMQLDQVERGFAYRTDAPLDMRMDATQGITAADILN
TYSHGDIARILKTYGDERFAGKIASAVLKEREKEPFTTSARLVELLYDAIPAATRRTGGHPAKRTFQALRVEVNNELDSL
KNVLPQITDALNVGGRAVFMSYQSHEDKLVKKFFTDLTTSKTPPGLPVDLPGTAPQFKQVTRGAETASEAEIEENPRAAP
VKVRAIERIANNSGDLS

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=282, Percent_Identity=35.4609929078014, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI165377202, Length=200, Percent_Identity=34.5, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI1786270, Length=327, Percent_Identity=37.6146788990826, Blast_Score=189, Evalue=2e-49,
Organism=Drosophila melanogaster, GI62472493, Length=343, Percent_Identity=33.2361516034985, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI28571637, Length=343, Percent_Identity=33.2361516034985, Blast_Score=154, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_CORGB (A4QFN1)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138949.1
- ProteinModelPortal:   A4QFN1
- STRING:   A4QFN1
- GeneID:   4992480
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2048
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 36800; Mature: 36800

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: BINDING 64-64 BINDING 98-98 BINDING 120-120 BINDING 127-127

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDFSLDGNHGHVPVMRDRMAALIAENVEALGENAVIVDATLGAGGHAEFFLNTFPKARL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCCCEE
IGLDRDQNALRDARARLAPFGERFIGVQTRFDGLREVLESVEGDIIDLAREHGIAGALFD
EECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHH
LGVSSMQLDQVERGFAYRTDAPLDMRMDATQGITAADILNTYSHGDIARILKTYGDERFA
HCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHH
GKIASAVLKEREKEPFTTSARLVELLYDAIPAATRRTGGHPAKRTFQALRVEVNNELDSL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KNVLPQITDALNVGGRAVFMSYQSHEDKLVKKFFTDLTTSKTPPGLPVDLPGTAPQFKQV
HHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHH
TRGAETASEAEIEENPRAAPVKVRAIERIANNSGDLS
HHCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEDFSLDGNHGHVPVMRDRMAALIAENVEALGENAVIVDATLGAGGHAEFFLNTFPKARL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCCCEE
IGLDRDQNALRDARARLAPFGERFIGVQTRFDGLREVLESVEGDIIDLAREHGIAGALFD
EECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHH
LGVSSMQLDQVERGFAYRTDAPLDMRMDATQGITAADILNTYSHGDIARILKTYGDERFA
HCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHH
GKIASAVLKEREKEPFTTSARLVELLYDAIPAATRRTGGHPAKRTFQALRVEVNNELDSL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KNVLPQITDALNVGGRAVFMSYQSHEDKLVKKFFTDLTTSKTPPGLPVDLPGTAPQFKQV
HHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHH
TRGAETASEAEIEENPRAAPVKVRAIERIANNSGDLS
HHCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA