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The map label for this gene is truB

Identifier: 145295883

GI number: 145295883

Start: 2006999

End: 2007892

Strand: Direct

Name: truB

Synonym: cgR_1808

Alternate gene names: 145295883

Gene position: 2006999-2007892 (Clockwise)

Preceding gene: 145295878

Following gene: 145295893

Centisome position: 60.56

GC content: 59.51

Gene sequence:

>894_bases
ATGAATGCTCCTGCCCCTAAACCTGGACTCGTGATCGTCGACAAGCCCGCCGGAATGACATCCCATGACGTGGTGTCCAA
ATTGCGCCGCGCATTTTCCACCCGAAAAGTTGGCCACGCAGGCACCCTCGACCCCATGGCAACCGGCGTGTTAGTCGTCG
GAATTGAGCGCGGAACCCGCTTCCTGGCACACATGGTGGCCTCCACCAAAGCCTACGACGCCACCATTCGACTCGGCGCC
GCCACCAGCACCGATGATGCAGAAGGCGAGGTTATCTCCACAACAGACGCATCCGGCCTCGACCACAGGGCCATCCTTGC
TGAAATCGCCAACCTCACCGGCGACATCATGCAAAAACCCACCAAAGTCTCCGCCATCAAAATCGACGGCAAACGCGCCC
ACGAACGCGTCCGCGACGGCGAAGAAGTAGACATTCCCGCACGTCCCGTCACCGTCAGCGTCTTTGACGTGCTCGACTAC
CGCGTCGACAGTGAATTTTATGACTTAGATGTGCGCGTCCACTGCTCCTCCGGCACCTACATCCGCGCGCTCGCCCGCGA
CCTCGGCAACGCTTTGCAGGTCGGCGGCCACCTGACCGCGCTTAGGCGCACAGAGGTCGGCCCTTTTACGCTTAACGACG
CGACCCCCCTCTCCAAACTCCAAGAGAATCCAGAACTCTCCCTCAACCTCGACCAGGCACTCACCCGCAGTTACCCAGTC
CTTGACATCACCGAAGACGAAGGCGTTGACCTGTCCATGGGCAAATGGTTGGAACCTCGCGGACTGAAAGGCGTCCACGC
TGCAGTAACACCATCAGGAAAAGCCGTGGCGCTCATCGAAGAAAAGGGCAAACGCCTGGCCACCGTGTTTGTTGCTCACC
CCAACACTCTTTAG

Upstream 100 bases:

>100_bases
TACACCAATGGTGACTACCGATCCTTGAAGATCAGCCGGAACGCTGTCTAGTCCACTCCAAATATCCACTGTTTTAGACT
ACGGCATAGACTCAACAGAC

Downstream 100 bases:

>100_bases
TTGGTCTGCCAGAAGCCGATTCAAGAGGTCGAATTTCGCTAGATAGGTGTGAGTGTGGAAAGAGCGGTTTGGAGGGCTTA
AACGCGATTCTGAGAGGGCA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNAPAPKPGLVIVDKPAGMTSHDVVSKLRRAFSTRKVGHAGTLDPMATGVLVVGIERGTRFLAHMVASTKAYDATIRLGA
ATSTDDAEGEVISTTDASGLDHRAILAEIANLTGDIMQKPTKVSAIKIDGKRAHERVRDGEEVDIPARPVTVSVFDVLDY
RVDSEFYDLDVRVHCSSGTYIRALARDLGNALQVGGHLTALRRTEVGPFTLNDATPLSKLQENPELSLNLDQALTRSYPV
LDITEDEGVDLSMGKWLEPRGLKGVHAAVTPSGKAVALIEEKGKRLATVFVAHPNTL

Sequences:

>Translated_297_residues
MNAPAPKPGLVIVDKPAGMTSHDVVSKLRRAFSTRKVGHAGTLDPMATGVLVVGIERGTRFLAHMVASTKAYDATIRLGA
ATSTDDAEGEVISTTDASGLDHRAILAEIANLTGDIMQKPTKVSAIKIDGKRAHERVRDGEEVDIPARPVTVSVFDVLDY
RVDSEFYDLDVRVHCSSGTYIRALARDLGNALQVGGHLTALRRTEVGPFTLNDATPLSKLQENPELSLNLDQALTRSYPV
LDITEDEGVDLSMGKWLEPRGLKGVHAAVTPSGKAVALIEEKGKRLATVFVAHPNTL
>Mature_297_residues
MNAPAPKPGLVIVDKPAGMTSHDVVSKLRRAFSTRKVGHAGTLDPMATGVLVVGIERGTRFLAHMVASTKAYDATIRLGA
ATSTDDAEGEVISTTDASGLDHRAILAEIANLTGDIMQKPTKVSAIKIDGKRAHERVRDGEEVDIPARPVTVSVFDVLDY
RVDSEFYDLDVRVHCSSGTYIRALARDLGNALQVGGHLTALRRTEVGPFTLNDATPLSKLQENPELSLNLDQALTRSYPV
LDITEDEGVDLSMGKWLEPRGLKGVHAAVTPSGKAVALIEEKGKRLATVFVAHPNTL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=222, Percent_Identity=37.8378378378378, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI215599015, Length=207, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI4503337, Length=207, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI2367200, Length=214, Percent_Identity=36.9158878504673, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17553978, Length=209, Percent_Identity=28.7081339712919, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323204, Length=205, Percent_Identity=32.6829268292683, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324037, Length=187, Percent_Identity=29.4117647058824, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281364189, Length=203, Percent_Identity=33.0049261083744, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364187, Length=203, Percent_Identity=33.0049261083744, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364185, Length=203, Percent_Identity=33.0049261083744, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364183, Length=203, Percent_Identity=33.0049261083744, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI62471759, Length=203, Percent_Identity=33.0049261083744, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17975520, Length=203, Percent_Identity=33.0049261083744, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_CORGB (A4QEY6)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138704.1
- ProteinModelPortal:   A4QEY6
- SMR:   A4QEY6
- STRING:   A4QEY6
- GeneID:   4994252
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1808
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   ASTKAYD
- ProtClustDB:   PRK03287
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR015225
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09142 TruB_C; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 31847; Mature: 31847

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: ACT_SITE 44-44

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPAPKPGLVIVDKPAGMTSHDVVSKLRRAFSTRKVGHAGTLDPMATGVLVVGIERGTR
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEEECHHHH
FLAHMVASTKAYDATIRLGAATSTDDAEGEVISTTDASGLDHRAILAEIANLTGDIMQKP
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
TKVSAIKIDGKRAHERVRDGEEVDIPARPVTVSVFDVLDYRVDSEFYDLDVRVHCSSGTY
CEEEEEEECCHHHHHHHCCCCCCCCCCCCEEEEHEEHHHHHCCCEEEEEEEEEEECCCCH
IRALARDLGNALQVGGHLTALRRTEVGPFTLNDATPLSKLQENPELSLNLDQALTRSYPV
HHHHHHHHHHHHHHCCEEEEEEECCCCCEEECCCCCHHHHHCCCCEEEEHHHHHHCCCCE
LDITEDEGVDLSMGKWLEPRGLKGVHAAVTPSGKAVALIEEKGKRLATVFVAHPNTL
EEECCCCCCEECCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEECCCCC
>Mature Secondary Structure
MNAPAPKPGLVIVDKPAGMTSHDVVSKLRRAFSTRKVGHAGTLDPMATGVLVVGIERGTR
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEEECHHHH
FLAHMVASTKAYDATIRLGAATSTDDAEGEVISTTDASGLDHRAILAEIANLTGDIMQKP
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
TKVSAIKIDGKRAHERVRDGEEVDIPARPVTVSVFDVLDYRVDSEFYDLDVRVHCSSGTY
CEEEEEEECCHHHHHHHCCCCCCCCCCCCEEEEHEEHHHHHCCCEEEEEEEEEEECCCCH
IRALARDLGNALQVGGHLTALRRTEVGPFTLNDATPLSKLQENPELSLNLDQALTRSYPV
HHHHHHHHHHHHHHCCEEEEEEECCCCCEEECCCCCHHHHHCCCCEEEEHHHHHHCCCCE
LDITEDEGVDLSMGKWLEPRGLKGVHAAVTPSGKAVALIEEKGKRLATVFVAHPNTL
EEECCCCCCEECCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA