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The map label for this gene is sigB [H]

Identifier: 145295824

GI number: 145295824

Start: 1939958

End: 1940953

Strand: Direct

Name: sigB [H]

Synonym: cgR_1749

Alternate gene names: 145295824

Gene position: 1939958-1940953 (Clockwise)

Preceding gene: 145295823

Following gene: 145295825

Centisome position: 58.54

GC content: 57.73

Gene sequence:

>996_bases
ATGACAGCACCGTCCACGCAGGATCTCGCAACGACTGAACGTGAGGTAGATCCCGGCAGCAGAAGGGGCCAAACCAACGA
CAATCCCTCGCAGGACCTTGTTCGCGTTTACCTCAACGGCATCGGCAAAACTGCCCTCCTTACCGCGGAGGATGAAGTTG
AGCTCGCACAGACCATTGAGGTTGGTCTTTATGCAGAGCACCTTCTGAAAAACTCTGAAGAGCCACTCACCCGCGCCATG
AAGCGCGATCTAAAGGTTCTTGCCAAGGACGGCAAGAAGGCTCGTTCCCACCTCCTCGAGGCAAACCTGCGCCTGGTGGT
GTCCCTTGCTAAGCGCTACACCGGCCGCGGCATGCCACTGCTGGATCTTATCCAGGAGGGCAACCTCGGACTGATCCGCG
CGATGGAAAAGTTTGATTACTCCAAGGGCTTTAAGTTCTCCACCTACGCAACCTGGTGGATCCGCCAGGCAATCACCCGC
GGCATGGCAGATCAGTCCCGCACCATCCGCCTCCCAGTCCACCTTGTGGAGCAGGTGAACAAGCTTTCCCGCATCAAGCG
CGAGATGTACCAGCACTTGGGCCGTGAAGCCACCAATGAGGAACTCGCAGAAGAGTCCGGCATTGAAGAGTCCAAGATTG
AAATGCTGCTTCGTCAGTCTCGCGATCCAGTGAGCTTGGACATGCCAGTCGGCGCCGATGAAGAAGCTCCTTTGGGTGAC
TTCATTGAGGATTCTGAGGCCACCGATGCAGAATCCGCAGTGGTTGCTTCCATGCGCCACTCCGATATCCGCGCGGTCCT
TAACACTCTCGAGCCACGCGAGCAGGATGTCATCCGTTTGCGCTACGGCCTCGATGACGGTGTGCCACGCACCCTTGATC
AGATTGGTCGCCGCTTCGGACTGTCCCGTGAGCGCGTCCGCCAGATCGAGCGTGAAGTCATGAGTAAGCTCCGCGATGGA
GAGCGCGCATCACGTCTTCGTGAGTACGCCCAGTAA

Upstream 100 bases:

>100_bases
CCCCAATGTGGGGGTGTTAAGAACTCCACAAAAGCGCTTGGGAACTTTTTGTGGAAGCAGTCCGTTGAACCTCTTGAACC
GCGAATTTAGGAGGCCAGTT

Downstream 100 bases:

>100_bases
AAGCGTGAACTAACAATCGTAGCGTCGGGGCCCACCTACCGTGGCCTCGGCGTTTTAGTGGTTCCTGGACCATATTTTAC
CGGCGAGTCCCAAGGCGTGA

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTAPSTQDLATTEREVDPGSRRGQTNDNPSQDLVRVYLNGIGKTALLTAEDEVELAQTIEVGLYAEHLLKNSEEPLTRAM
KRDLKVLAKDGKKARSHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYSKGFKFSTYATWWIRQAITR
GMADQSRTIRLPVHLVEQVNKLSRIKREMYQHLGREATNEELAEESGIEESKIEMLLRQSRDPVSLDMPVGADEEAPLGD
FIEDSEATDAESAVVASMRHSDIRAVLNTLEPREQDVIRLRYGLDDGVPRTLDQIGRRFGLSRERVRQIEREVMSKLRDG
ERASRLREYAQ

Sequences:

>Translated_331_residues
MTAPSTQDLATTEREVDPGSRRGQTNDNPSQDLVRVYLNGIGKTALLTAEDEVELAQTIEVGLYAEHLLKNSEEPLTRAM
KRDLKVLAKDGKKARSHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYSKGFKFSTYATWWIRQAITR
GMADQSRTIRLPVHLVEQVNKLSRIKREMYQHLGREATNEELAEESGIEESKIEMLLRQSRDPVSLDMPVGADEEAPLGD
FIEDSEATDAESAVVASMRHSDIRAVLNTLEPREQDVIRLRYGLDDGVPRTLDQIGRRFGLSRERVRQIEREVMSKLRDG
ERASRLREYAQ
>Mature_330_residues
TAPSTQDLATTEREVDPGSRRGQTNDNPSQDLVRVYLNGIGKTALLTAEDEVELAQTIEVGLYAEHLLKNSEEPLTRAMK
RDLKVLAKDGKKARSHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYSKGFKFSTYATWWIRQAITRG
MADQSRTIRLPVHLVEQVNKLSRIKREMYQHLGREATNEELAEESGIEESKIEMLLRQSRDPVSLDMPVGADEEAPLGDF
IEDSEATDAESAVVASMRHSDIRAVLNTLEPREQDVIRLRYGLDDGVPRTLDQIGRRFGLSRERVRQIEREVMSKLRDGE
RASRLREYAQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=251, Percent_Identity=49.003984063745, Blast_Score=260, Evalue=8e-71,
Organism=Escherichia coli, GI1789098, Length=316, Percent_Identity=38.9240506329114, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1789871, Length=238, Percent_Identity=31.0924369747899, Blast_Score=98, Evalue=7e-22,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 37574; Mature: 37443

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPSTQDLATTEREVDPGSRRGQTNDNPSQDLVRVYLNGIGKTALLTAEDEVELAQTIE
CCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
VGLYAEHLLKNSEEPLTRAMKRDLKVLAKDGKKARSHLLEANLRLVVSLAKRYTGRGMPL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LDLIQEGNLGLIRAMEKFDYSKGFKFSTYATWWIRQAITRGMADQSRTIRLPVHLVEQVN
HHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHH
KLSRIKREMYQHLGREATNEELAEESGIEESKIEMLLRQSRDPVSLDMPVGADEEAPLGD
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCH
FIEDSEATDAESAVVASMRHSDIRAVLNTLEPREQDVIRLRYGLDDGVPRTLDQIGRRFG
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHC
LSRERVRQIEREVMSKLRDGERASRLREYAQ
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
TAPSTQDLATTEREVDPGSRRGQTNDNPSQDLVRVYLNGIGKTALLTAEDEVELAQTIE
CCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
VGLYAEHLLKNSEEPLTRAMKRDLKVLAKDGKKARSHLLEANLRLVVSLAKRYTGRGMPL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
LDLIQEGNLGLIRAMEKFDYSKGFKFSTYATWWIRQAITRGMADQSRTIRLPVHLVEQVN
HHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHH
KLSRIKREMYQHLGREATNEELAEESGIEESKIEMLLRQSRDPVSLDMPVGADEEAPLGD
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCH
FIEDSEATDAESAVVASMRHSDIRAVLNTLEPREQDVIRLRYGLDDGVPRTLDQIGRRFG
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHC
LSRERVRQIEREVMSKLRDGERASRLREYAQ
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910; 2116013 [H]