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The map label for this gene is rpoD [H]

Identifier: 145295815

GI number: 145295815

Start: 1930993

End: 1932477

Strand: Direct

Name: rpoD [H]

Synonym: cgR_1740

Alternate gene names: 145295815

Gene position: 1930993-1932477 (Clockwise)

Preceding gene: 145295814

Following gene: 145295816

Centisome position: 58.26

GC content: 57.85

Gene sequence:

>1485_bases
ATGGTAGAAAACAACGTAGCAAAAAAGACGGTCGCTAAAAAGACCGCACGCAAGACCGCACGCAAAGCAGCCCCGCGCGT
GGCAACCCCATTGGGAGTCGCATCTGAGTCTCCCATTTCGGCCACCCCTGCGCGCAGCATCGATGGAACCTCAGCCCCTG
TTGAAGCTGCTGACACCATAGAGACCACCGCCCCTGCAGCGAAGGCTCCTACGGCCAAGGCTCCCGCTAAAAAGGTTGCC
AAGAAGACAGCTCGCAAGGCACCTGCGAAAAAGACTGTCGCCAAGAAGGCCACAACCGCCAAGGCTGCACCTGCAACTGC
CAAGGACGAAAACGCACCTGTTGATGACGACGAGGAGAACCTCCCTCAGGATGAACAGGACTTCGACGGCGATGACTTCG
TAGACGGCATCGAAGACGAAGAAGATGAAGACGGCGTCGAAGCCCTCGGTGAAGAAGGCGAAGACGACGAAGAGGACGGC
TCATCCGTTTGGGATGAAGACGAATCCGCAACCCTGCGTCAGGCACGTAAAGATGCCGAGCTCACCGCTTCCGCCGACTC
TGTTCGCGCTTACCTGAAGCAAATCGGTAAAGTTGCCCTGCTGAACGCTGAACAGGAAGTCTCCCTGGCAAAGCGCATCG
AAGCAGGCCTTTACGCCACCCACCGCATGGAGGAAATGGAAGAAGCTTTCGCAGCCGGTGACAAGGACGCGAAACTCACC
CCAGCCGTCAAGCGTGACCTCCGCGCCATCGCTCGTGACGGCCGCAAGGCGAAAAACCACCTCCTGGAAGCCAACCTTCG
TCTGGTTGTCTCCCTGGCAAAGCGCTACACCGGCCGTGGCATGGCATTCCTGGACCTCATCCAGGAAGGCAACCTCGGTC
TGATTCGTGCCGTAGAGAAGTTCGACTACTCCAAGGGCTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCA
ATCACCCGCGCCATGGCCGACCAAGCACGAACCATCCGTATCCCAGTCCACATGGTTGAAGTGATCAACAAACTTGGTCG
CATCCAACGTGAACTCCTTCAGGAACTCGGCCGCGAACCAACCCCACAGGAACTGTCCAAAGAAATGGACATCTCCGAGG
AAAAGGTACTGGAAATCCAGCAGTACGCCCGCGAACCAATCTCCCTGGACCAAACCATCGGCGACGAAGGCGACAGCCAG
CTCGGCGACTTCATCGAAGACTCCGAAGCCGTCGTCGCAGTTGACGCCGTCTCCTTCACCCTGCTGCAAGACCAGCTACA
GGACGTCCTAGAGACCCTCTCCGAACGTGAAGCCGGCGTGGTTAAACTCCGCTTCGGACTCACCGACGGAATGCCACGCA
CTTTAGACGAAATCGGCCAAGTTTACGGTGTCACCCGTGAGCGCATCCGCCAGATTGAGTCCAAGACCATGTCTAAGCTG
CGCCACCCATCACGCTCCCAGGTCCTTCGCGACTACCTGGACTAA

Upstream 100 bases:

>100_bases
TAGGCGGAAAGGGCGTTAAGTACTTGCCATTTAATCCTCAGCATCACTCGGATCAGTCGGAGATGTCGATGAAAATGCAC
CAGGAGCCGTGGAGAGCAGC

Downstream 100 bases:

>100_bases
AACCCCAGTCGGGCTCAAGACCGGGCCGCCACTGTTTTCCTCTGCGGGGAACGGTGGTGGCCCGGTTTTTCTGTTGCTTT
GGTTCGGCTGGTCACAGTTC

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MVENNVAKKTVAKKTARKTARKAAPRVATPLGVASESPISATPARSIDGTSAPVEAADTIETTAPAAKAPTAKAPAKKVA
KKTARKAPAKKTVAKKATTAKAAPATAKDENAPVDDDEENLPQDEQDFDGDDFVDGIEDEEDEDGVEALGEEGEDDEEDG
SSVWDEDESATLRQARKDAELTASADSVRAYLKQIGKVALLNAEQEVSLAKRIEAGLYATHRMEEMEEAFAAGDKDAKLT
PAVKRDLRAIARDGRKAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYSKGYKFSTYATWWIRQA
ITRAMADQARTIRIPVHMVEVINKLGRIQRELLQELGREPTPQELSKEMDISEEKVLEIQQYAREPISLDQTIGDEGDSQ
LGDFIEDSEAVVAVDAVSFTLLQDQLQDVLETLSEREAGVVKLRFGLTDGMPRTLDEIGQVYGVTRERIRQIESKTMSKL
RHPSRSQVLRDYLD

Sequences:

>Translated_494_residues
MVENNVAKKTVAKKTARKTARKAAPRVATPLGVASESPISATPARSIDGTSAPVEAADTIETTAPAAKAPTAKAPAKKVA
KKTARKAPAKKTVAKKATTAKAAPATAKDENAPVDDDEENLPQDEQDFDGDDFVDGIEDEEDEDGVEALGEEGEDDEEDG
SSVWDEDESATLRQARKDAELTASADSVRAYLKQIGKVALLNAEQEVSLAKRIEAGLYATHRMEEMEEAFAAGDKDAKLT
PAVKRDLRAIARDGRKAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYSKGYKFSTYATWWIRQA
ITRAMADQARTIRIPVHMVEVINKLGRIQRELLQELGREPTPQELSKEMDISEEKVLEIQQYAREPISLDQTIGDEGDSQ
LGDFIEDSEAVVAVDAVSFTLLQDQLQDVLETLSEREAGVVKLRFGLTDGMPRTLDEIGQVYGVTRERIRQIESKTMSKL
RHPSRSQVLRDYLD
>Mature_494_residues
MVENNVAKKTVAKKTARKTARKAAPRVATPLGVASESPISATPARSIDGTSAPVEAADTIETTAPAAKAPTAKAPAKKVA
KKTARKAPAKKTVAKKATTAKAAPATAKDENAPVDDDEENLPQDEQDFDGDDFVDGIEDEEDEDGVEALGEEGEDDEEDG
SSVWDEDESATLRQARKDAELTASADSVRAYLKQIGKVALLNAEQEVSLAKRIEAGLYATHRMEEMEEAFAAGDKDAKLT
PAVKRDLRAIARDGRKAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEKFDYSKGYKFSTYATWWIRQA
ITRAMADQARTIRIPVHMVEVINKLGRIQRELLQELGREPTPQELSKEMDISEEKVLEIQQYAREPISLDQTIGDEGDSQ
LGDFIEDSEAVVAVDAVSFTLLQDQLQDVLETLSEREAGVVKLRFGLTDGMPRTLDEIGQVYGVTRERIRQIESKTMSKL
RHPSRSQVLRDYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=241, Percent_Identity=59.7510373443983, Blast_Score=315, Evalue=4e-87,
Organism=Escherichia coli, GI1789098, Length=355, Percent_Identity=34.9295774647887, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI1789871, Length=231, Percent_Identity=29.4372294372294, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1788231, Length=204, Percent_Identity=28.921568627451, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 54333; Mature: 54333

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVENNVAKKTVAKKTARKTARKAAPRVATPLGVASESPISATPARSIDGTSAPVEAADTI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
ETTAPAAKAPTAKAPAKKVAKKTARKAPAKKTVAKKATTAKAAPATAKDENAPVDDDEEN
HHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHC
LPQDEQDFDGDDFVDGIEDEEDEDGVEALGEEGEDDEEDGSSVWDEDESATLRQARKDAE
CCCCCCCCCCCHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LTASADSVRAYLKQIGKVALLNAEQEVSLAKRIEAGLYATHRMEEMEEAFAAGDKDAKLT
HHCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PAVKRDLRAIARDGRKAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHH
FDYSKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQELGREP
HCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCC
TPQELSKEMDISEEKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFT
CHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHCCCCCEEEEHHHHHH
LLQDQLQDVLETLSEREAGVVKLRFGLTDGMPRTLDEIGQVYGVTRERIRQIESKTMSKL
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHPSRSQVLRDYLD
CCCCHHHHHHHHCC
>Mature Secondary Structure
MVENNVAKKTVAKKTARKTARKAAPRVATPLGVASESPISATPARSIDGTSAPVEAADTI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
ETTAPAAKAPTAKAPAKKVAKKTARKAPAKKTVAKKATTAKAAPATAKDENAPVDDDEEN
HHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHC
LPQDEQDFDGDDFVDGIEDEEDEDGVEALGEEGEDDEEDGSSVWDEDESATLRQARKDAE
CCCCCCCCCCCHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LTASADSVRAYLKQIGKVALLNAEQEVSLAKRIEAGLYATHRMEEMEEAFAAGDKDAKLT
HHCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PAVKRDLRAIARDGRKAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVEK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHH
FDYSKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQELGREP
HCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCC
TPQELSKEMDISEEKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFT
CHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHCCCCCEEEEHHHHHH
LLQDQLQDVLETLSEREAGVVKLRFGLTDGMPRTLDEIGQVYGVTRERIRQIESKTMSKL
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHPSRSQVLRDYLD
CCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]