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The map label for this gene is ruvC [H]

Identifier: 145295782

GI number: 145295782

Start: 1901581

End: 1902309

Strand: Reverse

Name: ruvC [H]

Synonym: cgR_1707

Alternate gene names: 145295782

Gene position: 1902309-1901581 (Counterclockwise)

Preceding gene: 145295783

Following gene: 145295781

Centisome position: 57.4

GC content: 53.22

Gene sequence:

>729_bases
TTGTGTGCTAAAAATGAGTATGGAATAGCAACTGCACAGCGACGAATCAGTGAGGACACGAATATGAACCACGAAGGGCT
TCGGGTAATGGGCATTGACCCAGGTCTTACCCGTTGTGGCTTATCAGTGGTTCAGGCAGGTCGTGGTCGTACCGTGTATC
CAGTGTCGGTAGGTGTGGTGCGCACCCCACCTGATGCGGAGTTGGCGGAGCGGTTGCTTCGGCTCAGCAAAGCAGTGGGT
GAGTGGATGGATGAGTACACCCCAGATGTCGTTGCTATTGAGCGTGTCTTTGAGCGCGGAAATGTTTCCACCGTGATGAA
CACTGCGCATGCGGTGGGTGTGTTGATCTTGGCTGCTGCTGAACGCGGATTGCCAGTTCACATGTACACCCCCAGTGAGG
TGAAAAAGGCTATCTCCGGTAATGGTCGCGCTGATAAGAAACAGATGACGGTCATGATCACTCGAATTCTGGGCCTTGGT
GAGCCACCCAAACCTGCTGACGCCGCTGATGCTTTATCGTTGGCGGTGTGTCACTGCTGGCGGGCGCCAATGCTTATGCG
GGCGCAATCGCAGTACTCCGAGCAAGAGTTGGAAAAGCGACGGCGTGTGCAGCAAGGAAAATTGGGTAAAGCGAAATCAA
CTTACAATGCGGAACAAGCTCAATCCCATGCATCCGATCCTGCTAAAGTCGCTCATCCCAGTCAGTTTCAACGAACTGAC
ACCAATTAG

Upstream 100 bases:

>100_bases
GTTTTGGCAGAGCTGGAAAGCGACTAGTTCGTATTTTCCGCACTCCGTTCACCCCGCTATCCCTGCAGCTAAAACGCAGA
GGATAGCGGGGTGATCGCAA

Downstream 100 bases:

>100_bases
TTTTTAAAAAGTTCTAGTAGATTTTTCAGGAGTGTCTTCATGATTGCCTCACTTCGTGGCACTGTTATCAACATTGGTCT
GAGCTCTGCTGTCATTGAAT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MCAKNEYGIATAQRRISEDTNMNHEGLRVMGIDPGLTRCGLSVVQAGRGRTVYPVSVGVVRTPPDAELAERLLRLSKAVG
EWMDEYTPDVVAIERVFERGNVSTVMNTAHAVGVLILAAAERGLPVHMYTPSEVKKAISGNGRADKKQMTVMITRILGLG
EPPKPADAADALSLAVCHCWRAPMLMRAQSQYSEQELEKRRRVQQGKLGKAKSTYNAEQAQSHASDPAKVAHPSQFQRTD
TN

Sequences:

>Translated_242_residues
MCAKNEYGIATAQRRISEDTNMNHEGLRVMGIDPGLTRCGLSVVQAGRGRTVYPVSVGVVRTPPDAELAERLLRLSKAVG
EWMDEYTPDVVAIERVFERGNVSTVMNTAHAVGVLILAAAERGLPVHMYTPSEVKKAISGNGRADKKQMTVMITRILGLG
EPPKPADAADALSLAVCHCWRAPMLMRAQSQYSEQELEKRRRVQQGKLGKAKSTYNAEQAQSHASDPAKVAHPSQFQRTD
TN
>Mature_242_residues
MCAKNEYGIATAQRRISEDTNMNHEGLRVMGIDPGLTRCGLSVVQAGRGRTVYPVSVGVVRTPPDAELAERLLRLSKAVG
EWMDEYTPDVVAIERVFERGNVSTVMNTAHAVGVLILAAAERGLPVHMYTPSEVKKAISGNGRADKKQMTVMITRILGLG
EPPKPADAADALSLAVCHCWRAPMLMRAQSQYSEQELEKRRRVQQGKLGKAKSTYNAEQAQSHASDPAKVAHPSQFQRTD
TN

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=159, Percent_Identity=32.7044025157233, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 26465; Mature: 26465

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAKNEYGIATAQRRISEDTNMNHEGLRVMGIDPGLTRCGLSVVQAGRGRTVYPVSVGVV
CCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEEE
RTPPDAELAERLLRLSKAVGEWMDEYTPDVVAIERVFERGNVSTVMNTAHAVGVLILAAA
ECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ERGLPVHMYTPSEVKKAISGNGRADKKQMTVMITRILGLGEPPKPADAADALSLAVCHCW
CCCCEEEEECHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
RAPMLMRAQSQYSEQELEKRRRVQQGKLGKAKSTYNAEQAQSHASDPAKVAHPSQFQRTD
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHCCCCCCCCCC
TN
CC
>Mature Secondary Structure
MCAKNEYGIATAQRRISEDTNMNHEGLRVMGIDPGLTRCGLSVVQAGRGRTVYPVSVGVV
CCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEEE
RTPPDAELAERLLRLSKAVGEWMDEYTPDVVAIERVFERGNVSTVMNTAHAVGVLILAAA
ECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ERGLPVHMYTPSEVKKAISGNGRADKKQMTVMITRILGLGEPPKPADAADALSLAVCHCW
CCCCEEEEECHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
RAPMLMRAQSQYSEQELEKRRRVQQGKLGKAKSTYNAEQAQSHASDPAKVAHPSQFQRTD
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHCCCCCCCCCC
TN
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]