Click here to switch to the map view.

The map label for this gene is pyrB [H]

Identifier: 145295734

GI number: 145295734

Start: 1848571

End: 1849509

Strand: Reverse

Name: pyrB [H]

Synonym: cgR_1661

Alternate gene names: 145295734

Gene position: 1849509-1848571 (Counterclockwise)

Preceding gene: 145295735

Following gene: 145295733

Centisome position: 55.81

GC content: 58.04

Gene sequence:

>939_bases
ATGAAGCACCTCCTATCCATTAGCGATCTTTCCAAAGATGAGATTGTTGGATTGCTGGATGAAGCGGATCGCTTTAAGGA
GGTGCTCGAAGGACGTGAAGTAAAGAAGCTGCCCACGCTGCGTGGTCGCACCATATTTACCTTGTTCTATGAGAACTCCA
CGCGCACCCGTTCGTCCTTTGAAACCGCAGGAAAATGGATGAGCGCCGATGTGATTAACATTTCGGCCTCATCATCCAGC
GTGAAGAAGGGCGAGTCGCTGAAAGATACCGGCTTGACTTTGTCGGCAATCGGCGCGGATGCGATCATTATGCGCCACCC
AGCCTCAGGCGCCGCGCAGCAGCTTGCGCAGTTCGTCGCACCAGGCGGCAACGGCCCCAGCGTGATCAACGCGGGTGACG
GTTCGCACCAGCACCCCACCCAGGCGCTTCTCGACGCTTTAACCATCCGGCAGCGCACCGGCCGCATTGAGGGACTCAAA
GTTGTCATCGTGGGCGACTGTTTGCACTCCCGCGTGGTGCGCTCCAATGTGGATCTGCTGTCCACCTTGGGCGCGGAGGT
GGTTCTGGTTGCTCCTCCAACACTGCTTCCCATGGGTGTGGAGAACTGGCCAGTCCGCTTCTCCTACGACATGGACGCAG
AAATTGCCGACGCCGACGTAGTGATGATGCTGCGCGTTCAACAAGAACGCATGCAGGGCGGATTCTTCCCCTCACACCGT
GAGTACGCAACGCTGTACGGCATGTCCAAAGAGCGCGAAGCTCGCCTCAAGGACTCCGCTATCATCATGCACCCCGGCCC
CATGCTTCGTGGCATGGAAATCAACTTCCAGGTGGCAGACGCACCACGCACCGCGGTACTGCAGCAGGTAAGCAACGGTG
TGCACATGCGCATGGCCATTTTGTTCGCCCTGGTCGCAGGCTCTGACGCGACTATCTGA

Upstream 100 bases:

>100_bases
CCCCACCGCACGCGCGGAAGATGTTTCCGTCATGCTTGCGGAAATCGACGGCCGCGACGCAGTCACGCTCACCCGAGAAG
ACTCTGAAGGGGATTCCTAG

Downstream 100 bases:

>100_bases
TCGCGACCATCTGATCGCGAAATTCTAGAAAAGGTAACGAGGGATTAAAGAAGTGGTTGACAGCAACACCCAGTATCCAG
AGACCGGCGCACTGGCACCG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKHLLSISDLSKDEIVGLLDEADRFKEVLEGREVKKLPTLRGRTIFTLFYENSTRTRSSFETAGKWMSADVINISASSSS
VKKGESLKDTGLTLSAIGADAIIMRHPASGAAQQLAQFVAPGGNGPSVINAGDGSHQHPTQALLDALTIRQRTGRIEGLK
VVIVGDCLHSRVVRSNVDLLSTLGAEVVLVAPPTLLPMGVENWPVRFSYDMDAEIADADVVMMLRVQQERMQGGFFPSHR
EYATLYGMSKEREARLKDSAIIMHPGPMLRGMEINFQVADAPRTAVLQQVSNGVHMRMAILFALVAGSDATI

Sequences:

>Translated_312_residues
MKHLLSISDLSKDEIVGLLDEADRFKEVLEGREVKKLPTLRGRTIFTLFYENSTRTRSSFETAGKWMSADVINISASSSS
VKKGESLKDTGLTLSAIGADAIIMRHPASGAAQQLAQFVAPGGNGPSVINAGDGSHQHPTQALLDALTIRQRTGRIEGLK
VVIVGDCLHSRVVRSNVDLLSTLGAEVVLVAPPTLLPMGVENWPVRFSYDMDAEIADADVVMMLRVQQERMQGGFFPSHR
EYATLYGMSKEREARLKDSAIIMHPGPMLRGMEINFQVADAPRTAVLQQVSNGVHMRMAILFALVAGSDATI
>Mature_312_residues
MKHLLSISDLSKDEIVGLLDEADRFKEVLEGREVKKLPTLRGRTIFTLFYENSTRTRSSFETAGKWMSADVINISASSSS
VKKGESLKDTGLTLSAIGADAIIMRHPASGAAQQLAQFVAPGGNGPSVINAGDGSHQHPTQALLDALTIRQRTGRIEGLK
VVIVGDCLHSRVVRSNVDLLSTLGAEVVLVAPPTLLPMGVENWPVRFSYDMDAEIADADVVMMLRVQQERMQGGFFPSHR
EYATLYGMSKEREARLKDSAIIMHPGPMLRGMEINFQVADAPRTAVLQQVSNGVHMRMAILFALVAGSDATI

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=36.0128617363344, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI2367364, Length=323, Percent_Identity=36.8421052631579, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1786469, Length=200, Percent_Identity=30.5, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790703, Length=200, Percent_Identity=31, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=333, Percent_Identity=35.4354354354354, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322331, Length=320, Percent_Identity=36.25, Blast_Score=156, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24642586, Length=324, Percent_Identity=34.5679012345679, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 33913; Mature: 33913

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLLSISDLSKDEIVGLLDEADRFKEVLEGREVKKLPTLRGRTIFTLFYENSTRTRSSF
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEEEECCCCCHHHH
ETAGKWMSADVINISASSSSVKKGESLKDTGLTLSAIGADAIIMRHPASGAAQQLAQFVA
HHHCCEEECEEEEEECCCCCHHCCCCCHHCCCEEEECCCCEEEEECCCCHHHHHHHHHHC
PGGNGPSVINAGDGSHQHPTQALLDALTIRQRTGRIEGLKVVIVGDCLHSRVVRSNVDLL
CCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHCCHHHH
STLGAEVVLVAPPTLLPMGVENWPVRFSYDMDAEIADADVVMMLRVQQERMQGGFFPSHR
HHCCCCEEEEECCCEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
EYATLYGMSKEREARLKDSAIIMHPGPMLRGMEINFQVADAPRTAVLQQVSNGVHMRMAI
CEEEEECCCCHHHHHCCCCEEEECCCCCEECEEEEEEECCCCHHHHHHHHHCCHHHHHHH
LFALVAGSDATI
HHHHHCCCCCCC
>Mature Secondary Structure
MKHLLSISDLSKDEIVGLLDEADRFKEVLEGREVKKLPTLRGRTIFTLFYENSTRTRSSF
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEEEECCCCCHHHH
ETAGKWMSADVINISASSSSVKKGESLKDTGLTLSAIGADAIIMRHPASGAAQQLAQFVA
HHHCCEEECEEEEEECCCCCHHCCCCCHHCCCEEEECCCCEEEEECCCCHHHHHHHHHHC
PGGNGPSVINAGDGSHQHPTQALLDALTIRQRTGRIEGLKVVIVGDCLHSRVVRSNVDLL
CCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHCCHHHH
STLGAEVVLVAPPTLLPMGVENWPVRFSYDMDAEIADADVVMMLRVQQERMQGGFFPSHR
HHCCCCEEEEECCCEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
EYATLYGMSKEREARLKDSAIIMHPGPMLRGMEINFQVADAPRTAVLQQVSNGVHMRMAI
CEEEEECCCCHHHHHCCCCEEEECCCCCEECEEEEEEECCCCHHHHHHHHHCCHHHHHHH
LFALVAGSDATI
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]