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The map label for this gene is thiC

Identifier: 145295445

GI number: 145295445

Start: 1513572

End: 1515335

Strand: Reverse

Name: thiC

Synonym: cgR_1381

Alternate gene names: 145295445

Gene position: 1515335-1513572 (Counterclockwise)

Preceding gene: 145295447

Following gene: 145295440

Centisome position: 45.72

GC content: 56.92

Gene sequence:

>1764_bases
ATGACGCCTACCCAAAATGAGATCCACCCGAAACACAGCTACTCCCCCATCCGCAAGGACGGTCTCGAGGTCCCGGAGAC
CGAAATCCGCCTCGATGACTCGCCAAGCGGCCCCAACGAACCCTTCCGCATCTACCGCACCCGTGGCCCAGAAACCGACC
CCAAGCAGGGACTTCCGCGCCTGCGCGAGTCATGGATCACCGCCCGCGGCGACGTTGCCACCTATCAGGGGCGCGAGCGT
TTGCTTATCGACGACGGCCGCTCGGCAATGCGTCGAGGTCAAGCTTCGGCAGAGTGGAAAGGCCAAAAACCAGCTCCTTT
GAAGGCGCTACCTGGCAAAAGAGTCACCCAAATGGCCTATGCGCGTGCTGGCGTGATTACTCGTGAAATGGAGTTTGTGG
CGCTGCGCGAACACGTTGATGCGGAGTTTGTGCGCTCTGAGGTGGCGCGCGGTCGGGCCATTATTCCCAACAACGTCAAC
CACCCCGAATCTGAACCGATGATTATTGGTCGCAAATTTTTGACCAAAATCAACGCAAATATTGGCAATTCTGCGGTCAC
CTCTTCAATCGAGGAAGAGGTGTCCAAGCTGCAGTGGGCCACGCGCTGGGGTGCCGATACCGTGATGGATCTATCCACCG
GCGATGATATTCACACCACCCGCGAATGGATTATCCGCAACTCCCCCGTTCCCATCGGCACCGTACCGATCTACCAAGCG
CTGGAAAAAGTAAATGGCGTGGCTGCCGATCTCACCTGGGAAGTATTCCGCGATACCGTCATTGAGCAGTGCGAACAAGG
CGTGGACTATATGACCATCCACGCCGGCGTCCTGCTGGCTTATATCCCACTGACTACCCGTCGTGTCACCGGCATTGTCT
CCCGCGGCGGATCCATTATGGCCGGTTGGTGTCTGGCGCACCACCGCGAATCATTTCTCTACGAGCATTTCGACGAGCTG
TGCAAAATCTTTGCACAATATGACGTCGCATTCTCCCTTGGTGATGGTCTACGCCCCGGCTCGCTTGCCGACGCCAACGA
TGCCGCGCAATTCGCCGAGCTGAAAACCATTGGTGAACTCACCCAACGCGCCTGGGAATACGATGTGCAAGTAATGGTCG
AAGGACCTGGACACGTGCCACTAAACATGATCCAAGAAAACAACGAGCTGGAACAAAAGTGGGCAGCGGACGCACCTTTT
TACACTCTTGGACCACTAGTTACCGACATCGCTCCAGGCTATGACCACATCACTTCTGCCATTGGTGCGGCTCACATCGC
CATGGGTGGCACCGCCATGCTGTGTTATGTCACCCCGAAAGAACACCTCGGTCTGCCCAACCGTGACGACGTCAAAACCG
GCGTAATCACCTACAAGCTCGCAGCCCACGCAGCCGATGTAGCCAAGGGTCATCCCGGCGCGCGTGCCTGGGACGACGCC
ATGAGCAAAGCGCGATTTGAATTCCGTTGGAATGATCAGTTTGCGCTCTCCCTCGACCCCGACACTGCAATCGCTTACCA
CGACGAAACCCTGCCGGCAGAGCCTGCCAAAACCGCGCACTTCTGTTCAATGTGTGGCCCTAAGTTCTGCTCCATGCGAA
TTAGCCAAGACATCCGCGATATGTTTGGCGATCAAATCGCGGAATTGGGGATGCCTGGGGTTGGGGATTCTTCTAGTGCT
GTTGCTTCTAGTGGGGCCCAGGAGGGGATGGCTGAGAAATCCCGGGAGTTTATTGCTGGTGGTGCGGAGGTTTATCGGCG
TTAG

Upstream 100 bases:

>100_bases
CACGGGCGCCCGAGCACGGGCTGAGATCGCGCTGATTGCTGCGCGAGCACCGTTTGAACCTGTCCGGTTAGCACCGGCGA
AGGAAGAGAGGAATGGTGCA

Downstream 100 bases:

>100_bases
ACAGAGCGATCCCAGATAGCCTCATATCGTTTTTCGACCAATGGCCAAATAATTTCCTGAACATCCTCCAATGTGGTGAT
GCCTTCCGCGAACTGCCCCC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MTPTQNEIHPKHSYSPIRKDGLEVPETEIRLDDSPSGPNEPFRIYRTRGPETDPKQGLPRLRESWITARGDVATYQGRER
LLIDDGRSAMRRGQASAEWKGQKPAPLKALPGKRVTQMAYARAGVITREMEFVALREHVDAEFVRSEVARGRAIIPNNVN
HPESEPMIIGRKFLTKINANIGNSAVTSSIEEEVSKLQWATRWGADTVMDLSTGDDIHTTREWIIRNSPVPIGTVPIYQA
LEKVNGVAADLTWEVFRDTVIEQCEQGVDYMTIHAGVLLAYIPLTTRRVTGIVSRGGSIMAGWCLAHHRESFLYEHFDEL
CKIFAQYDVAFSLGDGLRPGSLADANDAAQFAELKTIGELTQRAWEYDVQVMVEGPGHVPLNMIQENNELEQKWAADAPF
YTLGPLVTDIAPGYDHITSAIGAAHIAMGGTAMLCYVTPKEHLGLPNRDDVKTGVITYKLAAHAADVAKGHPGARAWDDA
MSKARFEFRWNDQFALSLDPDTAIAYHDETLPAEPAKTAHFCSMCGPKFCSMRISQDIRDMFGDQIAELGMPGVGDSSSA
VASSGAQEGMAEKSREFIAGGAEVYRR

Sequences:

>Translated_587_residues
MTPTQNEIHPKHSYSPIRKDGLEVPETEIRLDDSPSGPNEPFRIYRTRGPETDPKQGLPRLRESWITARGDVATYQGRER
LLIDDGRSAMRRGQASAEWKGQKPAPLKALPGKRVTQMAYARAGVITREMEFVALREHVDAEFVRSEVARGRAIIPNNVN
HPESEPMIIGRKFLTKINANIGNSAVTSSIEEEVSKLQWATRWGADTVMDLSTGDDIHTTREWIIRNSPVPIGTVPIYQA
LEKVNGVAADLTWEVFRDTVIEQCEQGVDYMTIHAGVLLAYIPLTTRRVTGIVSRGGSIMAGWCLAHHRESFLYEHFDEL
CKIFAQYDVAFSLGDGLRPGSLADANDAAQFAELKTIGELTQRAWEYDVQVMVEGPGHVPLNMIQENNELEQKWAADAPF
YTLGPLVTDIAPGYDHITSAIGAAHIAMGGTAMLCYVTPKEHLGLPNRDDVKTGVITYKLAAHAADVAKGHPGARAWDDA
MSKARFEFRWNDQFALSLDPDTAIAYHDETLPAEPAKTAHFCSMCGPKFCSMRISQDIRDMFGDQIAELGMPGVGDSSSA
VASSGAQEGMAEKSREFIAGGAEVYRR
>Mature_586_residues
TPTQNEIHPKHSYSPIRKDGLEVPETEIRLDDSPSGPNEPFRIYRTRGPETDPKQGLPRLRESWITARGDVATYQGRERL
LIDDGRSAMRRGQASAEWKGQKPAPLKALPGKRVTQMAYARAGVITREMEFVALREHVDAEFVRSEVARGRAIIPNNVNH
PESEPMIIGRKFLTKINANIGNSAVTSSIEEEVSKLQWATRWGADTVMDLSTGDDIHTTREWIIRNSPVPIGTVPIYQAL
EKVNGVAADLTWEVFRDTVIEQCEQGVDYMTIHAGVLLAYIPLTTRRVTGIVSRGGSIMAGWCLAHHRESFLYEHFDELC
KIFAQYDVAFSLGDGLRPGSLADANDAAQFAELKTIGELTQRAWEYDVQVMVEGPGHVPLNMIQENNELEQKWAADAPFY
TLGPLVTDIAPGYDHITSAIGAAHIAMGGTAMLCYVTPKEHLGLPNRDDVKTGVITYKLAAHAADVAKGHPGARAWDDAM
SKARFEFRWNDQFALSLDPDTAIAYHDETLPAEPAKTAHFCSMCGPKFCSMRISQDIRDMFGDQIAELGMPGVGDSSSAV
ASSGAQEGMAEKSREFIAGGAEVYRR

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=614, Percent_Identity=60.9120521172638, Blast_Score=733, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_CORGB (A4QDR7)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138266.1
- STRING:   A4QDR7
- GeneID:   4993169
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1381
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 64766; Mature: 64635

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: BINDING 180-180 BINDING 209-209 BINDING 238-238 BINDING 274-274 BINDING 374-374 BINDING 401-401

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTQNEIHPKHSYSPIRKDGLEVPETEIRLDDSPSGPNEPFRIYRTRGPETDPKQGLPR
CCCCCCCCCCCCCCCCHHHCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCCHHHCCHH
LRESWITARGDVATYQGRERLLIDDGRSAMRRGQASAEWKGQKPAPLKALPGKRVTQMAY
HHHHHHCCCCCEEEECCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ARAGVITREMEFVALREHVDAEFVRSEVARGRAIIPNNVNHPESEPMIIGRKFLTKINAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEHHHHHHHHCCC
IGNSAVTSSIEEEVSKLQWATRWGADTVMDLSTGDDIHTTREWIIRNSPVPIGTVPIYQA
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHEECCCCCCCCCHHHHH
LEKVNGVAADLTWEVFRDTVIEQCEQGVDYMTIHAGVLLAYIPLTTRRVTGIVSRGGSIM
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEE
AGWCLAHHRESFLYEHFDELCKIFAQYDVAFSLGDGLRPGSLADANDAAQFAELKTIGEL
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
TQRAWEYDVQVMVEGPGHVPLNMIQENNELEQKWAADAPFYTLGPLVTDIAPGYDHITSA
HHHHHCCCEEEEEECCCCCCHHHHHCCCHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHH
IGAAHIAMGGTAMLCYVTPKEHLGLPNRDDVKTGVITYKLAAHAADVAKGHPGARAWDDA
HHHHHHCCCCCEEEEEECCHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHH
MSKARFEFRWNDQFALSLDPDTAIAYHDETLPAEPAKTAHFCSMCGPKFCSMRISQDIRD
HHHCEEEEEECCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
MFGDQIAELGMPGVGDSSSAVASSGAQEGMAEKSREFIAGGAEVYRR
HHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
TPTQNEIHPKHSYSPIRKDGLEVPETEIRLDDSPSGPNEPFRIYRTRGPETDPKQGLPR
CCCCCCCCCCCCCCCHHHCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCCHHHCCHH
LRESWITARGDVATYQGRERLLIDDGRSAMRRGQASAEWKGQKPAPLKALPGKRVTQMAY
HHHHHHCCCCCEEEECCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ARAGVITREMEFVALREHVDAEFVRSEVARGRAIIPNNVNHPESEPMIIGRKFLTKINAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEHHHHHHHHCCC
IGNSAVTSSIEEEVSKLQWATRWGADTVMDLSTGDDIHTTREWIIRNSPVPIGTVPIYQA
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHEECCCCCCCCCHHHHH
LEKVNGVAADLTWEVFRDTVIEQCEQGVDYMTIHAGVLLAYIPLTTRRVTGIVSRGGSIM
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEE
AGWCLAHHRESFLYEHFDELCKIFAQYDVAFSLGDGLRPGSLADANDAAQFAELKTIGEL
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
TQRAWEYDVQVMVEGPGHVPLNMIQENNELEQKWAADAPFYTLGPLVTDIAPGYDHITSA
HHHHHCCCEEEEEECCCCCCHHHHHCCCHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHH
IGAAHIAMGGTAMLCYVTPKEHLGLPNRDDVKTGVITYKLAAHAADVAKGHPGARAWDDA
HHHHHHCCCCCEEEEEECCHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCHHHHHH
MSKARFEFRWNDQFALSLDPDTAIAYHDETLPAEPAKTAHFCSMCGPKFCSMRISQDIRD
HHHCEEEEEECCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
MFGDQIAELGMPGVGDSSSAVASSGAQEGMAEKSREFIAGGAEVYRR
HHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA