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The map label for this gene is gltX

Identifier: 145295434

GI number: 145295434

Start: 1505056

End: 1506546

Strand: Direct

Name: gltX

Synonym: cgR_1371

Alternate gene names: 145295434

Gene position: 1505056-1506546 (Clockwise)

Preceding gene: 145295432

Following gene: 145295439

Centisome position: 45.41

GC content: 56.81

Gene sequence:

>1491_bases
ATGGCCTGCATGACTGATGTTCGTGTTCGTTTCTGCCCATCGCCCACCGGCACCCCACACGTTGGACTTGTGCGCACCGC
GCTGTTCAACTGGGCATATGCTCGCCACACTGGAGGCAAGTTAGTTTTCCGCATTGAGGACACTGACGCTGCCCGCGATT
CCGAGGAGTCTTACTCCGCCATCATCGATTCCCTGCGCTGGTTGGGAATGGACTGGGATGAGGGTGTGGAAAAGGGCGGC
CCGCATGAGCCCTACCGCCAGTCGCAGCGCAAGGACATCTATCAGGATGTGCTGAAGCAGCTTATCGACGCCGGCGAGGT
CTATCCTGCTTACTCCACTGCCGAAGAGGTTGAGGAGCGACACAAGGCTGCTGGCCGCGATCCTAAGCTTGGCTACGACA
ACTTTGACCGTGATCTCACCGAAGAGCAGGTCGCAGCATTTGAGGCTGAGGGGCGTAAGCCTGTGTGGCGTCTGCGCATG
CCAGAGCAGGATTGGAAGTGGACTGACCTGGTCCGCGGTGAAGTTGAGTTCAAGTCGTTCACCCAGCCTGACTTCGTGGT
TGCTCGTTCCAACGGTGAGCCTTTGTACACCCTGGTCAACCCTGTTGATGATGCGTTGATGGAAGTCACCCATGTGCTGC
GTGGTGAGGATCTTTTGCCATCGACTCCTCGTCAGCTTGCTCTGTATGAGGCGCTCAAGCGCATTGGCGTGGCAAAGGTC
ACCCCAGCCTTTGGTCACCTGCCATTCGTGATGGGTGAGGGCAACAAGAAGCTGTCTAAGCGTGATCCACAGTCCAGCCT
GTTCAACCACCGTGACAACGGCATCATCCCAGAGGGCATGCTCAACTACCTGGCGCTGCTGGGTTGGTCGCTGTCTGCTG
ATCAGGACATCTTCGGTGTTGATGAGTTGATCGCTAACTTTGATGTCGCTGACGTATTGGGCAACCCAGCGCGCTTTGAC
CAGAAGAAGCTCGAGGCTATCAATGCGGATCACATCCGACTGCTGGAGCCTAAGGATTTCGAGGCTCGCCTGCGCGCGTA
CATGACTGAGTACACCGAGTTCCCTGCGGATTACCCAGCTGAGAAGTTTGCCATTGCTGCGGAGCTGGTTCAGACCCGCA
TCAAGGTGCTCAGCGAGGCGTGGGATCTGCTGAAGTTCCTGGTCACCGCTGACGAAGATCTGGTGCTCAATGAGAAGGCT
GCCAAGAAGAACCTCAAGGAGACCGCTGTTGAGCCTCTCAACGCAGGTATCGCAGCGCTGGAGGCAGTGGAGGAGTGGAC
CACTCCAAACATTGAAGCAGCGTTGAACAAGGCTCTCATTGAGGATCTTGGTCTGAAGCCTCGCGTGGCGTTCGGTGCGT
TGCGCATTGGTATCTCCGGTGAGGCTGTATCCCCACCACTGTTTGAGTCCATGGAGCTTTTGGGCAAGGAATCCACGTTG
GTTCGTCTGAAGGCTACTCGTGAGCAGACCCCATTCGTGGTCGCTGAGTAA

Upstream 100 bases:

>100_bases
GTGTTGGGGGCAGAGGGGTTGCGGTGGGCAGACATGGTTAGCCATTCTAATCCCAGTAGAACTGGGGTAAACGCGCGGGC
GGTGGGTGCGGGCTATTAAC

Downstream 100 bases:

>100_bases
AACCTGTAGATGAACAAAAGCCCGCCTCCGTTCAGAATTTCTGAGGGAGGTAGGCTCTTTGCGTTTCGGATTTAGTTGTG
GAGGGAGACGTCGTAACGCA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MACMTDVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGKLVFRIEDTDAARDSEESYSAIIDSLRWLGMDWDEGVEKGG
PHEPYRQSQRKDIYQDVLKQLIDAGEVYPAYSTAEEVEERHKAAGRDPKLGYDNFDRDLTEEQVAAFEAEGRKPVWRLRM
PEQDWKWTDLVRGEVEFKSFTQPDFVVARSNGEPLYTLVNPVDDALMEVTHVLRGEDLLPSTPRQLALYEALKRIGVAKV
TPAFGHLPFVMGEGNKKLSKRDPQSSLFNHRDNGIIPEGMLNYLALLGWSLSADQDIFGVDELIANFDVADVLGNPARFD
QKKLEAINADHIRLLEPKDFEARLRAYMTEYTEFPADYPAEKFAIAAELVQTRIKVLSEAWDLLKFLVTADEDLVLNEKA
AKKNLKETAVEPLNAGIAALEAVEEWTTPNIEAALNKALIEDLGLKPRVAFGALRIGISGEAVSPPLFESMELLGKESTL
VRLKATREQTPFVVAE

Sequences:

>Translated_496_residues
MACMTDVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGKLVFRIEDTDAARDSEESYSAIIDSLRWLGMDWDEGVEKGG
PHEPYRQSQRKDIYQDVLKQLIDAGEVYPAYSTAEEVEERHKAAGRDPKLGYDNFDRDLTEEQVAAFEAEGRKPVWRLRM
PEQDWKWTDLVRGEVEFKSFTQPDFVVARSNGEPLYTLVNPVDDALMEVTHVLRGEDLLPSTPRQLALYEALKRIGVAKV
TPAFGHLPFVMGEGNKKLSKRDPQSSLFNHRDNGIIPEGMLNYLALLGWSLSADQDIFGVDELIANFDVADVLGNPARFD
QKKLEAINADHIRLLEPKDFEARLRAYMTEYTEFPADYPAEKFAIAAELVQTRIKVLSEAWDLLKFLVTADEDLVLNEKA
AKKNLKETAVEPLNAGIAALEAVEEWTTPNIEAALNKALIEDLGLKPRVAFGALRIGISGEAVSPPLFESMELLGKESTL
VRLKATREQTPFVVAE
>Mature_495_residues
ACMTDVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGKLVFRIEDTDAARDSEESYSAIIDSLRWLGMDWDEGVEKGGP
HEPYRQSQRKDIYQDVLKQLIDAGEVYPAYSTAEEVEERHKAAGRDPKLGYDNFDRDLTEEQVAAFEAEGRKPVWRLRMP
EQDWKWTDLVRGEVEFKSFTQPDFVVARSNGEPLYTLVNPVDDALMEVTHVLRGEDLLPSTPRQLALYEALKRIGVAKVT
PAFGHLPFVMGEGNKKLSKRDPQSSLFNHRDNGIIPEGMLNYLALLGWSLSADQDIFGVDELIANFDVADVLGNPARFDQ
KKLEAINADHIRLLEPKDFEARLRAYMTEYTEFPADYPAEKFAIAAELVQTRIKVLSEAWDLLKFLVTADEDLVLNEKAA
KKNLKETAVEPLNAGIAALEAVEEWTTPNIEAALNKALIEDLGLKPRVAFGALRIGISGEAVSPPLFESMELLGKESTLV
RLKATREQTPFVVAE

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=488, Percent_Identity=31.3524590163934, Blast_Score=199, Evalue=4e-51,
Organism=Escherichia coli, GI1788743, Length=483, Percent_Identity=33.9544513457557, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI226510925, Length=314, Percent_Identity=28.343949044586, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71993394, Length=500, Percent_Identity=28.8, Blast_Score=192, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6324540, Length=347, Percent_Identity=36.5994236311239, Blast_Score=213, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6321192, Length=230, Percent_Identity=27.8260869565217, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24665321, Length=487, Percent_Identity=32.0328542094456, Blast_Score=218, Evalue=7e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_CORGB (A4QDQ6)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138255.1
- ProteinModelPortal:   A4QDQ6
- SMR:   A4QDQ6
- STRING:   A4QDQ6
- GeneID:   4993573
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1371
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   WINNQYV
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 55663; Mature: 55531

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 260-260

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACMTDVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGKLVFRIEDTDAARDSEESYSA
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
IIDSLRWLGMDWDEGVEKGGPHEPYRQSQRKDIYQDVLKQLIDAGEVYPAYSTAEEVEER
HHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
HKAAGRDPKLGYDNFDRDLTEEQVAAFEAEGRKPVWRLRMPEQDWKWTDLVRGEVEFKSF
HHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHCCCCHHCCC
TQPDFVVARSNGEPLYTLVNPVDDALMEVTHVLRGEDLLPSTPRQLALYEALKRIGVAKV
CCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHC
TPAFGHLPFVMGEGNKKLSKRDPQSSLFNHRDNGIIPEGMLNYLALLGWSLSADQDIFGV
CCCCCCCCEEECCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
DELIANFDVADVLGNPARFDQKKLEAINADHIRLLEPKDFEARLRAYMTEYTEFPADYPA
HHHHHCCCHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCH
EKFAIAAELVQTRIKVLSEAWDLLKFLVTADEDLVLNEKAAKKNLKETAVEPLNAGIAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHH
EAVEEWTTPNIEAALNKALIEDLGLKPRVAFGALRIGISGEAVSPPLFESMELLGKESTL
HHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCEE
VRLKATREQTPFVVAE
EEEEECCCCCCEEEEC
>Mature Secondary Structure 
ACMTDVRVRFCPSPTGTPHVGLVRTALFNWAYARHTGGKLVFRIEDTDAARDSEESYSA
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
IIDSLRWLGMDWDEGVEKGGPHEPYRQSQRKDIYQDVLKQLIDAGEVYPAYSTAEEVEER
HHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
HKAAGRDPKLGYDNFDRDLTEEQVAAFEAEGRKPVWRLRMPEQDWKWTDLVRGEVEFKSF
HHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHCCCCHHCCC
TQPDFVVARSNGEPLYTLVNPVDDALMEVTHVLRGEDLLPSTPRQLALYEALKRIGVAKV
CCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHC
TPAFGHLPFVMGEGNKKLSKRDPQSSLFNHRDNGIIPEGMLNYLALLGWSLSADQDIFGV
CCCCCCCCEEECCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
DELIANFDVADVLGNPARFDQKKLEAINADHIRLLEPKDFEARLRAYMTEYTEFPADYPA
HHHHHCCCHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCH
EKFAIAAELVQTRIKVLSEAWDLLKFLVTADEDLVLNEKAAKKNLKETAVEPLNAGIAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHH
EAVEEWTTPNIEAALNKALIEDLGLKPRVAFGALRIGISGEAVSPPLFESMELLGKESTL
HHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCEE
VRLKATREQTPFVVAE
EEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA